Livin,a novel member of human inhibitor of apoptosis protein family,is highly expressed in various malignant tumors. It plays an important role in the initiation,development,treatment and prognosis of tumors.Many studies have proved that down-regulation of Livin expression using RNA interference technique can reduce tumor growth,increase the apoptotic rate,and sensitize tumor cells to radiotherapy and chemo therapy.Full-length Livin is cleaved by the effector caspases to produce a truncated form (tLivin) with apoptosis-promotion activity.tLivin provides a new direction for the treatment of the malignant tumors.

To observe the effect of PCEA on the serum endotheline and the renal function after operation in kidney transplantation patients. Forty patients with chronic renal failure were randomly divided into two groups: group P( n =20) and group N ( n =20) .Venous blood samples were drawn before anesthesia at the end of operation and 24, 48, 72h after operation. Mean arterial pressure (MAP), serum endothelin,creatinine, urea nitrogen, uric acid were measured with radioimmunoassay. The results showed that the MAP, serum endothelin, creatinine, urea nitrogen and uric acid were decreased significantly at 24h after operation.All of them reached the nadir at 72h after operation. But those of group N were significantly higher than group P ( P

OBJECTIVE:To study the bioequivalences of both domestic nevirapine capsules and imported nevirapine tablets.METHODS:24healthy male volunteers were administered orally with single dose of either the domestic nevirapine trial capsules or the imported nevirapine reference tablets by randomized crossover way,the blood concentration of nevirapine was determined by HPLC,and the pharmacokinetics parameters and the relative bioavailability of whom were calculated.RE?SULTS:The C max of the trial preparation and the reference preparation were(2.516?0.446)?g/ml and(2.798?0.394)?g/ml,respectively t max were(7.5?10.7)and(3.6?2.1)h,t 1/2 were(51.4?25.3)h and(46.4?9.8)h;AUC 0～168 were(160.540?38.007)(?g?n)/ml and(167.459?30.629)(?g?n)/ml;AUC 0～∞ were(180.064?51.005)(?g?n)/ml and(183.052?36.828)(?g?n)/ml;The relative bioavailability of the trial preparation was(98.368?22.99)%.CONCLUSION:The results shows that the trial preparation and the reference preparation of nevirapine were bioequivalent.

Objective: To analysis the medical expenses of six diseases and put forward the efficient methods to control their unreasonable rise. Methods: 22582 patients with six diseases in two largest hospitals in Xi'an were chosen and analyzed from 1/1/ 2003 to 10/1/2004. Results: Six diseases were the main reason of hospitalization and the malignancy was most important; drug expense of the uninsured patients, which was the key factor to induce the medical expenses rising, was higher than insured patients. Conclusion: The medical insurance program could control the drug expense efficiently; different control strategies to different diseases were important to distribute the medical resource and lighten the life burdens of the inpatients.

Objective　To investigate the repairing effect of transplantation of allogeneic fetal bone in combination with a covering cryopreserved periosteal allograft to bone defect. Methods　Twenty Long-eared white male rabbits were chosen as experimental model of bilateral 12 mm combined bony and periosteal radial defect. Cryopreserved allograft periosteum with allogeneic fetal bone were implanted in the left defect as experimental side and fetal bone was simply transplanted in the right defect as control side. Bone repair process in the two groups were compared by macroscopy, microscopy, roentgenograms and the contents of calcium and phosphate in the defect area at 2、4、8 and 12 weeks after transplantation. Results　There was significant statistic difference in the contents of calcium and phosphate between the experimental and control sides at 4、8 and 12 weeks after transplantation (P<0.05). With time passing by, the contents of calcium and phosphate have the increasing trends. In the experimental group, lamella bone was seen and medullary canal recanalized at 8 weeks postoperatively. The histological section showed the bone lacuna and lamella bone were formed. Conclusion　It suggests that allogeneic fetal bone in combination with a covering cryopreserved periosteal allograft can promote bone repair, and allogeneic fetal bone is excellent bone substitute.

Objective To observe the distribution and drug resistance of pathogens cultured from the sputum of hospitalized patients with lower respiratory infection in acute exacerbation of chronic obstructive pulmonary disease (AECOPD) .Methods To i‐dentify the germiculture and test the drug susceptibility of the sputum or respiratory secretion isolated from the bronchial brush of 262 hospitalized AECOPD patients in People′s Hospital of Jiangxi Province from Janurary 2013 to December 2014 and analyze the results .Results Among all the AECOPD patients ,215 cases with positive sputum culture ,281 sputum pathogens were isolated . Gram‐negative bacilli were found in 190(67 .6% ) .Gram‐positive aureus were detected in 76(27 .1% ) .Fungus pathogens occurred in 15(5 .3% ) .The top six pathogenic bacteria were acinetobacter baumannii ,escherichia coli ,klebsiella pneumonia ,pseudomonas aeruginosa ,staphylococcus aureus ,streptococcus pneumonia .Drug susceptibility results showed that the drug resistance of acineto‐bacter baumannii was the strongest .Except that the drug resistance rate of cefoperazone/sulbactam and levofloxacin were less than 50 .0% ,the others were no less than 75 .0% .The drug resistance rate of escherichia coli and klebsiella pneumoniae to ampicillin , ampicillin sulbactam ,cefazolin ,ceftriaxone ,cefotetan ,gentamycin ,ofloxacin ,ciprofloxacin ,and compound sulfamethoxazole trime‐thoprim were no less than 70 .0% .The drug resistance rate of staphylococcus aureus to penicillin G ,oxacillin ,erythromycin ,clinda‐mycin were 100% .The drug resistance rate of streptococcus pneumoniae to erythromycin ,clindamycin ,tetracycline ,sulfamethox‐azole trimethoprim were greater than 75 .0% .Conclusion Gram‐negative bacilli are the main pathogenic bacterium in the AECOPD patients with lower respiratory infection .The key of treatment is to pay more attention to the bacterial culture and drug sensitive test ,use antibiotics reasonably according to the results of drug sensitive experiment .

BACKGROUND:To maintain the long-term effect of ful crown largely depends on the health of periodontal tissues. OBJECTIVE:To explore the effect of CAD/CAM zirconia al-ceramic crown restoration on the state of periodontal health. METHODS:Sixty-four abutments of 55 patients were randomly divided into two groups:the experimental group included 32 abutments of 29 patients which would be restored by CAD/CAM zirconia al-ceramic crowns;the control ed group included 32 abutments of 26 patients which would be restored by Ni-Cr al oy porcelain-fused-to-metal restorations. The volume of gingival crevicular fluid and the levels of interleukin-6 and tumor necrosis factor-αin the two groups were examined at the pre-restoration and post-restoration stages. Meanwhile, periodontal clinical indicators, including sulcus bleeding index, probing depth, plaque index and attachment loss were recorded. RESULTS AND CONCLUSION:No difference in various indexes was found in the experimental group before and after restoration (P>0.05). At 12 months after restoration, in the control group, the volume of gingival crevicular fluid, the levels of interleukin-6 and tumor necrosis factor-α, sulcus bleeding index, probing depth, and plaque index were al increased (P>0.05);meanwhile, these indexes in the experimental group were significantly lower than those in the control group (P<0.05). Experimental findings suggest that the CAD/CAM zirconia al-ceramic crown restoration is more favorable to the health of periodontal tissues.

Objective To study the safety of laparoscopic cholecystectomy (LC) with low pressure pneumoperitoneum for high risk patients with gallstone. Methods 72 high risk patients with gallstones were undergone LC with low pressure pneumoperitoneum and their clinical data were analysed in our hospital during last three years. Results one of 72 patients was converted into open cholecystectomy due to bleeding, and two of them were converted due to severe adhesion. The others were performed successfully and no serious complications occurred. Conclusions LC is a safe method for high risk patients with gallstone. The key point is to use low pressure pneumoperitoneum during LC.

Objective To investigate the expression level of Ezrin and its clinical significance in nontraumatic os-teonecrosis of femoral head. Methods Expression level of Ezrin in 38 patients with nontraumatic osteonecrosis of femoral head ( experimental group) and 16 patients with femoral neck fractures ( control group) were detected by immunohistochemistry and RT-PCR analysis. Results The expression level of Ezrin was significantly lower in ex-perimental group than that in control group (P<0. 05). It was correlated with the Ficat Stage, and had no rela-tionship with gender, age, BMI, profession and etiological factor. Conclusion Ezrin may involve in the develop-ment of nontraumatic osteonecrosis of femoral head, and play an important role in regulation.