The characteristics of knowledge exchange in medical field of China were analyzed by social network analysis with the medical data covered in CNKI Citation Database as its study object, which showed that both the inflow and outflow volumes of clinical medicine are the largest and form the core in knowledge exchange network, preclinical medicine is the theoretical base of other medical subjects and the important knowledge source in medical networks, the interchange is close between traditional Chinese medicine, surgery and other clinical subjects, oncology is rather active in interdisciplinary studies, the interchange between stomatology and psychology is rather rare with other clinical subjects in knowledge networks and stomatology and psychology are two independent subjects.Carrying out information push service for these subjects by making use of their characteristics can improve the targeted information service, step up the knowledge exchange between different medical subjects, and promote the cooperative interdis-ciplinary studies.

Objective To identify the mediators of CDC42 signaling pathway involved in hepatitis B virus X protein (HBx)-mediated cellular transformation.Methods The mass defect-based pseudo-isobaric dimethyl labeling method (pIDL)was used to detect the differentially expressed proteins with a deficiency of CDC42.Furthermore,we conducted a gene ontology (GO)of differentially expressed proteins.Results and Conclusion We totally qualified 3409 proteins and found 220 differentially expressed proteins.Palladin,formin-like 1 (FMNL1)and keratin-19,which were implicated in cytoskeleton organization,were down-regulated with the deficiency of CDC42.Our results have provided candidate genes and proteins that may play an important role in HBx-mediated cellular transformation.

Cell division cycle 42 (CDC42)is a member of Rho guanosine triphosphatase (GTPase) family,which plays an important role in cell proliferation , cell migration and cell transformation .The activity of CDC42 can be regulated by guanine nucleotide exchange factors (GEFs),GTPase activating proteins (GAPs) and guanine nucleotide-dissociation inhib-itors (GDIs).Recently,CDC42 has been reported as abnormally expressed in many human cancers ,suggesting that CDC42 performs complex functions in tumorigenesis .Therefore,this paper aims to shed light on the functions of CDC 42 in cancers in terms of the alteration of CDC42 activity,CDC42 regulators,as well as its effectors.

Apnea ; epidemiology ; pathology ; therapy ; Birth Weight ; China ; epidemiology ; Female ; Gestational Age ; Humans ; Infant, Newborn ; Infant, Very Low Birth Weight ; Male ; Risk Factors ; Time Factors

In the modern biotechnology era,the important disease genetic resources become the most dependent factor.We expound the actuality of the collection,storage and use of the important disease genetic resources in China,and discuss the problems and challenges we meet.So the chief mission we should do is establishing the database and collecting,storing and using these valuable resources under standard procedure with aim,plan and organization.

Psoriasis is a kind of immune-mediated chronic inflammatory disease, characterized with skin and joints. This paper reviewed the effect of traditional Chinese medicine on thepsoriasis related cytokines, such as interleukin, interferong, tumor necrosis factorα, colony stimulating factor, chemokines and transforming factor, in order to provide theoretical reference for TCM treatment of psoriasis.

0.05).The addition of sIL-6R to group SFT6 restored UCB cells expansion to a higher extent than in group SFT,SFT6 and SFTs(P0.05).Conclusion:The IL-6/sIl-6R in combination with SCF,FL and TPO could enhance the expansion of cord blood CD34+ cells and their expression of CD49d,CD62L and CXCR4.

To observe the protective effect of somatostatin (SS) on gastric mucosa of rats.Methods: Before gastric mucosal lesion were made in rats by pure alcohol, SS was intravenously droppedand anti-SS serum was injected into the vein, and the change of ulcer index and the degree of gastric mu-cosal lesion were observed. Results: Ulcer index of the SS group was much lower than that of the controlgroup (P

Objective To elucidate the effect of ex vivo expansion of umbilical cord blood (UCB) mononuclear cells (MNCs) on their implantation capability and the hematopoietic reconstitution, and to find a feasible way of applying ex vivo expanded UCB MNCs to clinical transplantation.Methods UCB MNCs were cultured in short-term in serum-free medium with different early acting cytokines combinations in order to observe the amplification result and cells apoptotic difference. The 6-day expanded cells were transplanted into sublethally irradiated NOD/SCID mice to assess the implantation and the hematopoietic reconstitution of the survival mice.Results UCB MNCs reached the best amplification result between day 6 and day 10 with the contribution of SCF, FL, IL-6 and IL-3 in common and the presence of Annxin V on the surface of cells obviously decreased. Six weeks after transplantation, CD45, CD34, CD33, CD3 and CD19 antigen could be detected by FCM on BM, spleen and thymus cells of the alive mice and the human specific Alu and Cart-Ⅰ repetitive sequence could be detected in the DNA obtained from peripheral blood by PCR.Conclusion After 6-day effective expanding with SCF+FL+IL-6+IL-3 UCB MNCs can be implanted into NOD/SCID mice successfully and contribute to reconstitute the multiple hematopoiesis.

Objective To investigate the diagnostic value of 18F-fluorodeoxyglucose (FDG) PET/CT in the localization of ectopic adrenocorticotrophic hormone ( ACTH)-secreting tumors. Methods Seven patients with ectopic ACTH syndrome (confirmed by clinical symptom, biochemical data or bilateral simultaneous inferior petrosal sinus sampling) were included, 4 males and 3 females with age ranged 27-63 years old.All patients were examined with head MR, chest and abdomen CT, abdomen ultrasonography, and whole body 18F-FDG PET/CT. Location, size, and the maximum standardized uptake value (SUVmax) of the tumor were measured. Results 18 F-FDG PET/CT detected three ectopic ACTH-secreting tumors with the sizes 1. 1 cm×1.0 cm (SUVmax =9.4), 1.5 cm×1.1cm (SUVmax =6.4), and 1.6 cm ×1.5 cm (SUVmax =12.0). The pathological findings of the three tumors were thymoma, lung carcinoid, and mediastinal carcinoid respectively. Conclusion 18 F-FDG PET/CT may help the localization of ecto pic ACTH-secreting tumors .