By means of polyacry1amide gel slab electrophoresis,the peroxide isoenzyme and the so1uble protein ofvarious types of Gastrodia elata in different growing periods were studied. The results revealed that the appar-ent varieties of G. elata are not stable in heredity.

98%. Conclusion The developed method is simple, reproducible and easy to operate. The solvent is cheap, with low boiling point and easy to recovery property.

MinK-related peptides is a unique family of ion channel ancillary subunits,that modify voltage-gating,conductance and pharmacology of ion channel subunits.This article reviews MinK-related peptides on the gene and protein structure,subunits from a variety of subfamilies,and the potential role in physiology and pathology.

Colorectal cancer is a common malignancy in the digestive tract,open and laparoscopic surgery are the main treatment methods.Anastomotic fistula,anastomotic bleeding,ileus,voiding and sexual dysfunction are the common postoperative complications.Chylous fistula,incision infection,pulmonary infection and incision metastasis occasionally occurred.Compared with open surgery,laparoscopic surgery does not increase the postoperative complications,and it can be safely applied in the radical resection of colorectal cancer.

On the basis of the theories of air sterilization, the Virobuster steritubes are very effective in the removal and inactivation of airborne bacteria species, viral particles, fungi and spore by using the high-intensity UV lamp and close-in UVC treatment room with high reflecting sputtering aluminum, the sterilization efficiency were above 99.999%～99.999 9% when the atmosphere passed through the germicidal tubes. As a technical breakthrough, the Virobuster steritubes could overcome the problems of traditional air sterilization and decontamination technology, the air sterilization could reach LOG-6 sterilizing effect. By analyzing of the workings principles and structure characteristics of Virobuster steritube, the optimization design of air sterilization and decontamination is set up for our public buildings air and hospital sterile room.

AlM:To evaluate the clinical application of the domestic rebound tonometer ( RBT ) in measuring intraocular pressure ( lOP) in healthy children.
METHODS:lOP measurement was measured in bilateral eyes of 108 cases with domestic RBT. Then the lOP for the left eyes of the children older than 13 years was obtained with Goldmann applanation tonometry ( GAT) .
RESULTS: Of the 108 children, 100 ( 92. 6%) had lOP measurement both eyes successfully. Of the 100 children, 24 were older than 13 years. The mean lOP values for the left eyes of the 24 children of RBT and GAT were (16. 0± 3. 0) mmHg (1kPa=7. 5mmHg) and (15. 7±2. 8) mmHg, respectively. There was no significant difference between the lOP of the two tonometers (P>0. 05). The lOP of RBT had a good liner relationship with that of GAT ( r=0. 849, P<0. 05). The lOP values were normally distributed for the both eyes. Mean lOP values were (16. 0±2. 8) mmHg and (16. 2 ± 2. 6 ) mmHg for the left eyes and right eyes, respectively. There was no statistically significant difference in mean lOP readings between them, which had a good liner relationship (r=0. 863, P<0. 05).
CONCLUSlON: Domestic RBT is a good instrument in detecting lOP in children and it seems to be very comfortable and easy when performing lOP measurement in children without an anesthetic.

Objective To investigate the discussion of risk factors of severe head injury with multiple organ dysfunction syndrome.Methods The clinical data of 149 cases with severe traumatic brain injury patients were retro-spectively analyzed,and the risk factors in patients with severe traumatic brain injury were collected,which may affect MODS,such as gender,age,GCS score,associated injuries,chronic underlying diseases,infections and other details, statistically analyzed were combined with incidence of MODS.Results (1 )Univariate analysis results could be seen, age,chronic underlying diseases,admission GCS score,extensive brain parenchymal damage,merge large organ dam-age,brain herniation,concurrent infection,massive blood transfusion were risk factors of MODS severe traumatic brain injury(χ2 =12.962,11.725,11.021,13.894,11.486,12.031,12.967,13.078,P=0.023,0.025,0.027,0.017, 0.023,0.018,0.021,0.019).(2)MODS rate and the number of risk factors were positive correlation(r=0.528,P=0.021).(3)With the increase of the number of mortality in patients with MODS organ dysfunction occurred signifi-cantly increased,MODS patients in this group,the two organs mortality was 17.4%,the three organs were 46.7%, four or more mortality was 87.5%(χ2 =11.735,13.933,12.748,P=0.028,0.019,0.025).Conclusion Age, chronic underlying diseases,GCS low,extensive parenchymal injury,combined injury,the occurrence of hernia,com-plicated by infection,massive blood transfusion were independent risk factors for brain injury,which should be payed attention.

OBJECTIVE:To observe the distribution of fluorescent material in the respiratory tract of animals with the use of small animal imaging technology to quickly evaluate the effects of respiratory drug delivery. METHODS: The Kunming mice in the experimental group were administered with Cy5.5 fluorescent-labeled aerosol,meanwhile,a positive control group was set up by injecting same amount of compound anhydrous diethyl ether solution via tracheal incubation,with negative control group inhaled the same amount of non-Cy5.5 fluorescently-labeled aerosol. The fluorescent distribution of the aerosolized particles in the respiratory tract was observed. RESULTS: Fluorescently-labeled aerosol was observed in both the experimental group and the positive control group but not in negative control group. CONCLUSION: The small animal imaging technology is expected to be used as a rapid and effective method for evaluating the efficacy of respiratory drug delivery.

Objective To investigate the incidence, cause,prevention and treatment of early postoperative inflammatory intestinal obstruction(EPIIO)in patients undergoing laparoscopic and open radical resection of colorectal cancer. Methods From September 2000 to September 2006,483 cases of colorectal cancer were divided into two groups according the procedures received: laparoscopic surgery(232 cases)and open surgery(251 cases).The incidence of early postoperative intestinal obstruction was compared between the two groups. Results 1.In laparoscopic group,14 cases(5.69%,14/246)were converted to open surgery;2.The incidences of EPIIO in LS and OS group were respectively 3.02%(7/232)and 7.97%(20/251)(P＜0.05,x2=5.60＝.In LS group,6 EPIIO caseswere cured conservatively, one underwent surgery, while in open surgery group,18 cases recovered conservatively, two received surgery. Conclusions 1.Significantly less cases suffered from EPIIO in laparoscopic group.2.Most EPIIO cases will recover conservatively, in cases with repeated symptoms or when strangulation of bowel is suspected open surgery should be attempted.

Objective To explore the clinical value of tumor marker squamous cell carcinoma antigen(SCCA) ,tissue polypeptide specific antigen(TPS) ,neuron‐specific enolase(NSE) ,cytokeratin fragment 19(CYFRA21‐1) detections in the patient with lung cancer .Methods 70 patient with newly diagnosed lung cancer(including 26 cases of squamous cell carcinoma ,23 cases of adenocar‐cinoma and 21 cases of small cell carcinoma) ,40 cases of lung benign diseases and contemporaneous 30 individuals undergoing the physical examination were randomly selected as the lung cancer group ,lung benign disease group and healthy control group respec‐tively .Serum tumor markers of SCCA ,TPS ,NSE and CYFERA21‐1 were detected in all cases .Then the detection results were per‐formed the statistical analysis .Results The levels of SCCA ,TPS ,NSE and CYFERA21‐1 in the lung cancer group were significant‐ly higher than those in the lung benign diseases group and healthy control group ,the differences were statistically significant (P<0 .05) .The expression levels of serum SCCA ,TPS ,NSE and CYFERA21‐1 were different in different histological types of lung cancer .SCCA and CYFERA21‐1 were highly expressed in squamous cell carcinoma ,which showed the statistical difference com‐pared with other two pathological types of lung cancer(P<0 .05);the expression level of NSE was highest in small cell lung cancer , which showed the statistical difference compared with other two pathological types of lung cancer(PP<0 .05);the TPS expression level had no statistical difference among different pathological types of lung cancer(P>0 .05) .The combination detection of SCCA , TPS ,NSE and CYFERA21‐1 greatly increased the sensitivity and accuracy for diagnosing lung cancer .Conclusion The combina‐tion detection of SCCA ,TPS ,NSE and CYFERA21‐1 has very high clinical value for the diagnosis ,pathological typing and assisted clinical therapy of lung cancer .