In our previous studies, substance P-like immunoreactive varicose nerve fibers have been demonstrated in the pars distalis of the adenohypophysis of the dog. They were found, at light microscopical level, to be closely related to gland cells. In the present study, the ultrastructure of substance P-like immunoreactive nerve fibers and their relationship with the gland cells of the pars distalis in the dog were investigated by use of pre-embedding immuno-electron microscopy. Direct contacts could be ascertained on every cell type of the gland, including folliculo-stellate cells. Typical synapses were identified on somatotropes and corticotropes, more on the latter. Most of them were of asymmetrical type with round to oval small clear vesicles and scattered large dense cored vesicles. It is considered morphologically proved that the substance P-like immunoreactive nerve fibers have effector role in the pars distalis of the dog.

Objective:To investigate 7-11-years-old children's emotional responses, emotional attributions, coping styles to success and failure situation in learning. Methods: With clinical interview method ,90 pupils from seven-years-old to elevelen-years-old were asked to inference to the dramatis personae's affect reaction, emotional attribution in the given situation.Results:Children's emotional response and emotional attribution in learning context is self-regarded, coping style also decline to self effort .There were no age and sex differences in emotional responses to successful situation,there were age differences to failure situation,and there were no sex difference in emotional attribution.Conclusion:Children's emotional responses and emotional attribution to success and failure in learning have different features.

Single nucleotide polymorphism(SNP)is the single base pair variation in genomic DNA that occurs in more than 1% of the general population.SNPs sometimes cause structural and functional changes in protein encoded by the gene.In the treatment of malignant tumors,SNPs may be key factors in determining responses and prognosis of the treatment.Main genes that have been studied on the correlation of SNP with response and prognosis after the treatment of non-small cell lung cancer include:DNA excision and repair gene,matrix metalloproteinase gene,apoptosis related gene,drug metabolism related gene,cell cycle control gene,etc.This review will focus on the advances in correlation of single nucleotide polymorphism with response and prognosis of lung cancer after chemotherapy.

Objective To observe the distribution of the activated cerebral regions after acupuncture stimulation of Zhongzhu(SJ3) and Waiguan(SJ5) by functional magnetic resonance imaging(fMRI) technique.Methods A total of 8 cases of volunteer healthy subjects were recruited for this study.Before and during manipulation of the needle,cerebral images were sampled separately by fMRI scanner and analyzed by AFNI software.Results During manipulation of the needle inserted in the unilateral Zhongzhu(SJ3) and Wauguan(SJ5),the highly-activated cerebral structures were cingulated gyrus,temporal gyrus,cerebellum,occipital gyrus,etc.Conclusion The effect of acupuncture analgesia was probably produces by the interaction of multiple brain structures of functional connectivity rather than through the activation of a single brain region.

Objective To investigate the clinical efficacy of CRRT in the treatment of severe pancreatitis.Methods 120 patients with severe pancreatitis were divided into two groups according to the treatment.All the patients were given basic treatment in all groups.The Pa arterial oxygen pressure (PaO2),oxygenation index (PaO2/FiO2),and C-reactive protein (CRP) in the two groups after 72 hours of treatment were observed.The levels of blood lactate (Lac),APACHEII,tumor necrosis factor α(TNF-α),interleukin-6 (IL-6) and interleukin-1β (IL-1β) were measured before and after treatment 72 hours.A comparison of mortality and hospitalization time was observed in 28 days of treatment.Results After treatment,Lac [the control group (3.32±0.85)mmol/L,the observation group (2.55±0.65)mmol/L],APACHEII score [the control group (13.30±2.80)points,the observation group (12.01±2.60)points],CRP [the control group (24.30±2.80)mg/L,the observation group (12.33±1.60)mg/L] were significantly lower than before treatment [Lac:the control group (4.85±1.05)mmol/L,the observation group (4.90±1.02)mmol/L;APACHEII score:the control group (16.62±2.95)points,the observation group (16.90±3.01)points;CRP:the control group (40.32±3.10)mg/L,the observation group (40.40±3.51)mg/L;the control group:tLac=1.67,P=0.004,tAPACHEII=6.32,P=0.000,tCRP=29.71,P=0.000;the observation group:tLac=15.05,P=0.005,tAPACHEII=9.52,P=0.000,tCRP=56.36,P=0.000].PaO2 [the control group (75.30±4.80)mmHg,the observation group (84.31±4.60)mmHg], PaO2/FiO2 [the control group (225.30±14.83)mmHg,the observation group (256.31±14.65)mmHg] were significantly higher than before treatment [PaO2:the control group (60.32±4.15)mmHg,the observation group (60.40±4.01)mmHg;PaO2/FiO2:the control group (130.39±11.15)mmHg,the observation group (130.90±11.01)mmHg;the control group:tPaO2=18.29,P=0.000,tPaO2/FiO2=39.62,P=0.000;the observation group:tPaO2=30.35,P=0.000,tPaO2/FiO2=53.01,P=0.000].Those in the observation group were significantly improved than the control group (tLac=5.574,P=0.00,tAPACHEII score=2.615,P=0.005,tPaO2=3.646,P=0.0002,tPaO2/FiO2=11.523,P=0.00,tCRP=28.751,P=0.000).After treatment,the TNF-α,IL-6 and IL-1β levels in the two groups [the control group:IL-1β (70.32±6.85)ng/mL,IL-6 (103.30±8.80)ng/mL,TNF-α (89.30±8.80) ng/mL;the observation group:IL-1β(48.55±6.62)ng/mL,IL-6(92.01±8.60)ng/mL,TNF-α(57.31±7.60)ng/mL] were significantly improved than before treatment [the control group:IL-1β(82.85±7.05)ng/mL,IL-6(173.62±9.95)ng/mL,TNF-α (105.32±9.15)ng/mL;the observation group:IL-1β(83.90±7.32)ng/mL,IL-6 (175.90±10.01)ng/mL,TNF-α (106.40±9.01)ng/mL;the control group:tIL-1β=9.66,P=0.000,tIL-6=41.01,P=0.000,tTNF-α=9.77,P=0.000;the observation group:tIL-1β=27.74,P=0.000,tIL-6=49.23,P=0.000,tTNF-α=32.26,P=0.000].And those of the observation group improved more significantly than the control group (tIL-1β=17.702,P=0.00,tIL-6=7.107,P=0.00,tTNF-α=21.311,P=0.000).The mortality rate and hospitalization time of the observation group were 18.33% and (10.97±2.92)days,which were significantly lower than those of the control group [36.67%,(13.63±3.26)days;χ2=5.058,P=0.025;t=4.708,P=0.000).Conclusion The use of CRRT in the treatment of severe pancreatitis can improve the vital signs,reduce the inflammation index,improve the serum levels of inflammatory factors and lactic acid,reduce the mortality and hospital stay.

Objective To study the effects of B cells from lupus prone Triple congenic (TC) mouse model on the differentiation and development of T cell subsets. Methods The spleen size and B cell numbers were measured, and surface CD40, CD86 and Ⅰ-Ab molecules on B cells as well as CD4+T cell subsets were detected using flow cytometry when the spontaneous systemic lupus erythematosus (SLE) model TC mice and control B6 mice were 6 months old. In addition, the chimera of TC B cells and B6 CD4+T cells or chimera of B6 B cells and B6 CD4+ T cells were transferred into B6.Rag-/- mice via intravenous injection. Then, T cell subsets in the spleen of recipient B6.Rag-/-mice were observed 7 days after cell transplantation. Results TC mice had significantly bigger spleen [(5337±934) mg] and more CD19+B cell number [(276.0±48.7)×107] than control B6 mice [spleen weight: (91±4) mg; B cell number: (6.4±0.3)×107](P<0.01). TC mice showed markedly increased CD40 [MFI (63.6±3.1)], CD86 [(MFI (18.96±0.44)] and Ⅰ-Ab [MFI (637±41) on spleen B cells compared with that of B6 mice [CD40 MFI: (36.6 ±2.0); CD86 MFI: (14.26 ±0.19); Ⅰ-Ab MFI: (307 ±23)] (P<0.01). In addition, TC mice revealed notably more Th1 subset [(36.54 ±4.22)%] in spleen than B6 mice [Th1 subset: (19.90±0.10)%] [P<0.01], but both strains had equivalent percentages of Th17 and IL-21+CD4+T cell populations (P>0.05). The recipient B6.Rag-/-mice transplanted with TC B cells had significantly more Th1 subset [(54.1±2.8)%] and IL-21+CD4+T cell population [(14.3±1.0)%], but less Th17 subset [(2.05±0.09)%] in spleen than the recipient B6.Rag-/-mice administered by B6 B cells [Th1 subset: (39.5±1.1)%; IL-21+CD4+T cell population:(7.5±1.2)%;Th17 subset:(6.45±1.10)%](P<0.01). Conclusion The B cells of lupus-prone TC mice exhibit a markedly hyper-activation in spleen, and promote CD4+T cells differentiation preferentially into Th1 subset and IL-21+CD4+T cell population, which may further contribute to SLE pathogenesis.

Aged ; Female ; Humans ; Neoplasms, Multiple Primary ; pathology ; physiopathology ; Neoplasms, Second Primary ; pathology ; physiopathology

ObjectiveTo investigate the inhibition of telomerase activity of colorectal cancer by chemotherapeutic drugs.MethodsBy using telomerase repeat amplification protocol (TRAP) combined with PAGE silver staining, we detected the telomerase activity of human colorectal cancer cell line HT-29 under the effect of cisplatin, doxorubicin, pirarubicin, mitomycin C, and 5-FU.ResultsIn high doses,no inhibition of tolemerase activity was found when cells were collected after only 4 hours of drug treatment, but the telomerase activity was completely inhibited by cisplatin when the drug was removed and cells were reculured for 20 hours. However, doxorubicin, pirarubicin, mitomycin C, 5-FU had no such effect. ConclusionCisplatin inhibits telomerase activity of human colon cancer cell HT-29, while other drugs had no such effect.

Objective To investigate the clinical and pathological characteristics as well as the surgical treatment of perforated gastric carcinoma (PGC). Methods The clinical and pathological data, surgical treatment and survival time of 48 patients with PGC were retrospectively analyzed. Results The mean age was 58 years in the 48 patients with PGC. There were 3 tumors in stage I, 11 in stage II, 21 in stage III , and 13 in stage IV. Emergency gastrectomy was done in 29 patients, with the mortality was 6.9%. Six patients underwent local repair at first, gastrectomy was done 3 weeks later without postoperative death. Perforation repair with or without gastroenterostomy was performed in 13 patients, with the mortality was 23.1% .The median survival time in gastrectomy group was 28 months (ranged 13 to 72 months) and in repair group was 7 months (ranged 3 to 10 months). Conclusions Emergency gastrectomy is the choice of treatment for PGC. If the conditions is not suitable for emergent gastrectomy ,staging operation should be adoptted to reduce operative mortality.

Calcium blockers verapamil ( VER ) and nifedipine ( NIF ) appa-rentely inhibited the spontaneous activity of isolated rat oviduct. The 5 min contractile curve aera determined by integrator,after eithet VER or NIF effected, was smaller than that before,with dose dependence.In rats pregnanant for 3d VER and NIF accelerated ovum transport through oviduct. The number of ovum recovered from uterus in test group was more than that in control group. Paired experiments showed VER and NIF having no anti-implantation action.