BACKGROUND:Raloxifene is the third generation of selective estrogen receptor modulators, which can decrease bone loss, increase bone mineral content, and reduce fracture risk. OBJECTIVE: To study the effects of raloxifene combined with self-setting calcium phosphate cement on the repair of rabbit mandibular defects. METHODS:Totaly 36 New Zealand white rabbits were selected to prepare 8 mm×4 mm×3 mm mandibular defect models, and then randomized equaly into experimental group (raloxifene, 7.5 mg/kg per day, combined with self-setting calcium phosphate cement), drug group (raloxifene, 7.5 mg/kg per day), artificial bone group (self-setting calcium phosphate cement). Rabbits were sacrificed 4, 8 and 12 weeks later, respectively, for measurement of bone morphogenetic protein 2 using immunohistochemistry method and transforming growth factor β using a laser scanning confocal microscope. RESULTS AND CONCLUSION:After 4 and 8 weeks, the expression of bone morphogenetic protein 2 was obviously higher in the experimental group than the drug and artificial bone groups; after 12 weeks, bone remodeling was basicaly complete in the experimental group, and the expression of bone morphogenetic protein 2 became lower than that in the other two groups. The expression of transforming growth factor β in the experimental group was gradualy increased and reached the peak at 8 weeks, while in the drug and artificial bone groups, the expression of transforming growth factor β exhibited an increasing trend within 4-12 weeks, which was close to the peak. These findings suggest that raloxifene can promote early expression of bone morphogenetic proteins and early calus formation as wel as accelerate the repair of bone defects with calcium phosphate cement.

Objective To compare the surgical effects of laparoscopy and open mini-incision surgery in cholelithotomy with the gallbladder being preserved.MethodsFrom April 2006 to May 2008,63 cases of cholelithiasis received cholelithotomy with the gallbladder being preserved in our hospital by laparoscopy(n=33) or mini-incision open surgery(n=30).The operation time,recovery time of gastrointestinal function,rates of postoperative analgesics use and complications,inpatient charge,and drug expense after the operation were analyzed.ResultsThe mean operation time in laparoscopy group was significantly longer than that in open group [(66.4?10.6) min vs(60.6?9.8) min,t=2.248,P=0.020)].And the inpatient charge in laparoscopy group was significantly higher than that in the open group [(7865?992) yuan vs(6995?825) yuan,t=3.765,P=0.000)].Whereas,the recovery time of the gastrointestinal tract and postoperative hospital stay in the laparoscopy group was significantly shorter than those in the other [(22.7?6.9) h vs(36.9?17.2) h,t=-4.374,P=0.000);(4.8?1.5)d vs(10.0?7.4) d,t=-3.951,P=0.000)].In the laparoscopy group,only one patient showed complication(periumbilical infection) after the treatment,while in the open group,2 patients developed incisional hematoma,2 showed incisional infection,3 had pelvic effusion,and 1 suffered from adhesive intestinal obstruction(?2=5.369,P=0.020).ConclusionsLaparoscopy is a good choice for cholelithotomy with the gallbladder being preserved,for it is minimally invasive,and thus leads to less surgical trauma,quicker recovery,fewer complications,and shorter hospital stay after the procedure.

Objective To investigate the efficacy of microwave ablation for big liver cancer by using laparoscopy.MethodsFrom August 2000 to October 2006,28 patients with large big liver cancer(over 5 cm in diameter)was treated by microwave ablation under the guidance of a laparoscope.On each tumor nodule,3 to 5 electrode insertions was applied.And for each lesion,the treatment was performed for 1 to 3 times.Results The complete ablation rate of this series was 78.6%(22/28),and the rate of local recurrence was 35.7%(10/28).1-,3-,and 5-year survival rates were 75.0%(21/28),57.1%(12/21),and 36.4%(4/11),respectively.Conclusions Laparoscopic microwave ablation is safe and effective for liver cancer.The procedure can increased the efficacy of the microwave by enlarging the extent of the ablation.

Objective To explore the evaluation of three-dimensional fast imaging employing steady-state acquisition(3D-FIESTA) sequence in the diagnosis of non-tumorous midbrain aqueduct stenosis.Methods 48 patients with non-tumorous midbrain aqueduct stenosis were performed by conventional SE sequence and 3D-FIESTA sequence on a 1.5T superconductive MR unit.Then the advantage on showing aqueduct of midbrain foramen was retrospectively analyzed.Results The detecting rate of midbrain aqueduct stenosis was 98% (47/48) on 3D-FIESTA sequence and 79% (38/48) on conventional SE sequence,3D-FIESTA was more advanced on showing the aqueduct of midbrain(P <0.01).Conclusion The combination of 3D-FIESTA with MPR can clearly show the midbrain aqueduct stenosis,and be a new MRI examination way of obstructive hydrocephalus.

Objective To explore the change and clinical significance of serum Cystain-C in children with Henoch-Schonlein purpura(HSP).Methods Thirty-three children diagnosed HSP according to the domestic criteria hospitalized in department of pediatrics were enrolled in patient group and 31 children outpatients during the corresponding time period as controls.Venous blood of each child in 2 groups was collected in the day of admission,serum Cystain-C was determined by enzyme-linked immunosorbent assay,plasma D-dimer was measured by gold standard double antibody sandwich method,and blood urea nitrogen(BUN) and serum creatinine(Scr) were detected by automatic biochemistry analyzer.At the same time each child undertook routine urine examination.Data was analyzed by Stata 8.0 software.Results 1.BUN and Scr of 2 groups were both normal.2.Proteinuria or hematuria was found in 15 of all 33 patients through routine urianlysis,and the detection rate was 45%.3.The pre-and post-therapy level of serum cystain-C[(3.83?0.83) mg/L,(3.76?1.02) mg/L] and plasma D-dimer [(1.93?2.05) mg/L,(0.77?0.79) mg/L] in HSP group were higher than that in controls [(1.21?0.43) mg/L,(0.13?0.07) mg/L] significantly (P0.05).5.In HSP group,the level of plasma D-dimer pretherapy was significantly higher than that of post-therapy(P0.05).Conclusion The level of serum Cystain-C can act as the significant indicator of early diagnosis and continuous observation of HSP nephritis.

Objective To calculate the social economic burden of mental illness,and to analyze the economic impact of different patients with mental illness in Shandong Province.Methods Direct method was used to calculate the direct economic burden,and human capital method was employed to estimate the indirect economic burden.Results The social economic burden increased from 10.076 billion in 2005 to 31.277 billion in 2013,and the proportion of the social economic burden accounting for GDP of Shandong province was between 0.5 ％ ～ 0.7％.The economic burden caused by women,18-39 years old patients,rural patients and mood disorders was higher than that of men,more than 55 years-old patients,urban patients and other diagnoses,respectively.Conclusions The economic burden brought by mental illness was heavy and increasing during 2005-2013.The economic burden caused by different people was heterogeneous.

Objective To explore the efficacy and safety of the treatment of Xuezhikang and fluvastatin in elderly angina patients with mildly abnormal liver function .Methods 84 cases of ALT in 40-80u/L very elderly patients with coronary heart disease were randomly selected and divided into the two groups , the Xuezhikang group received Xuezhikang (0.6g,bid,orally),the fluvastatin group received fluvastatin (40mg,1time/night,oral),the total course was 12weeks,the TC,TG,LDL-C,HDL -C were measured before and after treatment ,liver function was measured once 2 -4times.If ALT was 3 times higher than before , Xuezhikang was instead of statins , if ALT was 5 times higher than before ,both of the two drags were withdrawaled .Results The TC,LDL,TG,HDL-C and ALT of the two groups were no significant difference before treatment (P >0.05),the TC,LDL,TG were decreased after treatment in the both group .There was no case of increased of ALT to three times in Xuezhikang group ,however ,ALT of 6 patients were increased more than three times in fluvastatin group ,four weeks after exchange of Xuezhikang ,the ALT were not continued to rise .Conclusion Both the two medicine can significantly lowered the cholesterol in very elderly patients with angina ,but the Xuezhikang were more safety in the patient with mild increased of liver function .

OBJECTIVETo systematically assess the efficacy and safety of Rhodiola in treating chronic stable angina pectoris.

METHODSOur group searched the Cochrane library, PubMed, Embase, Chinese biomedical literature database (CBM), VIP database (VIP), Chinese Journal Full-text Database (CNKI) for the literature published in English and Chinese till April 2013. Randomized controlled trials (RCTs) were included on the therapeutic effect of Rhodiola or Rhodiola plus conventional Western medicine in comparison with the conventional Western medicine treatment on stable angina. Data were extracted according the data extraction form. The literature methodological quality was assessed by using the Cochrane handbook, and data analyzed by Rev-Man 5.2 Software for Meta-analysis. The effect indicators of outcomes was expressed by odds ratio (OR) and 95% CI.

RESULTSA total of 7 randomized controlled trials, 662 cases of stable angina pectoris patients met the inclusion criteria and all published in Chinese, without one scientific design and high quality literature. Compared with the conventional Western medicine treatment, combined with oral administration of Rhodiola could improve the efficiency of anti-angina (OR = 2.49, 95% CI: 1.02 - 6.09). Combined with intravenous infusion of Rhodiola could also improve the efficacy of angina pectoris (OR = 4.86, 95% CI: 2.4 - 9.82). Oral administration of Rhodiola couldn't improve ECG efficacy (OR = 1.25, 95% CI: 0.67 - 2.34). Intravenous infusion of Rhodiola could improve the clinical efficacy (OR = 2.94, 95% CI: 1.61 - 5.35). Combined with the conventional treatment, intravenous infusion of Rhodiola could improve the whole blood viscosity (low and high shear rates) and inverse variance (IV) (-1.36 and -0.99, 95% CI: -1.65 - 1.07 and -1.26 - 0.71), but could not reduce serum fibrinogen and D-dimer level. The incidence rate of adverse reactions was higher than that of the conventional treatment combined with Rhodiola (OR = 0.1, 95% CI: 0.02 - 0.51).

CONCLUSIONSOn the basis of routine treatment, Rhodiola could further improve patients' symptoms. Combined with intravenous medication, Rhodiola could increase the ECG improvement rate, and reduce adverse reactions. But the methodological quality of included studies was poor, the number of samples was small, and influence factors such as the intervention period was short. This conclusion needs scientific and rational design in a larger sample, multicenter clinical trial to verify.

Human herpesvirus 7 (HHV-7) infection is dependent on the functions of structural glycoproteins at multiple stages of the viral life cycle. These proteins mediate the initial attachment and fusion events that occur between the viral envelope and a host cell membrane, as well as cell to cell spread of the virus. To characterize the HHV-7 glycoproteins that can mediate cell fusion, a cell-based fusion assay was used. 293T cells expressing the HHV-7 glycoproteins of interest along with a luciferase reporter gene under the control of the T7 promoter were cocultivated with SupT1 cells transfected with T7 RNA polymerase. HHV-7 glycoproteins gB, gH, gL and gO can mediate the fusion of 293T cells with SupT1 cells, and the fusion can be inhibited by anti-CD4 mAbs. Thus, the coexpression of HHV-7 gB, gO, gH and gL is sufficient and necessary for HHV-7 induced membrane fusion, and one of these glycoproteins or protein complex formed by these glycoproteins might be the ligand(s) of CD4 molecule.

Objective To observe and analyse the therapeutic effect of pentoxifylline in treating traumatic cerebral infarction.Methods Besides routine treatment received in control group,PTX 0.2～0.6 injection plus 500ml normal saline was intravenously dripped once a day,in the treatment group.Duration ten days as a treatment course in both groups and three courses were carried out.N-acetylaspartate(NAA),choline(Cho),Creatine(Cr) and lactate(Lac) in infarct part of brain were measured with proton magnetic resonance spectroscopy(1HMRS).Results Compared with control group,ability of daily life were better,N-acetyl aspartate(NAA) and creatine(Cr) were obvious higher.Lactate(Lac) were obvious lower,in the treatment group.Conclusion PTX has a better therapeutic effect in traumatic cerebral infarction.