Crystal HRP was applied to the cut ends of the left infraorbital nerve and branches of the facial nerve of 34 adult mice. Then the animals were killed and perfused, and HRP solution was applied to the cut ends of the root of the right facial nerve and the right maxillary nerve. Frozen sections of the brain stem and the trigerminal ganglia were made and reacted with BDHC, TMB or DAB method for light and electrom microscopic examination.Under light microscopy, the neurons labeled when alive in the left trigerminal ganglion and the nucleus of the left facial nerve showed that more cell bodise but less nerve fibers were labeled. The reaction products with all the 3 methods in thess cells were granular. The neurons labeled after death in the right trigerminal ganglion and the nucleus of the right facial nerve showed that more fibers but less cell bodies were labeled. The reaction products in these cells were also granular with BDHC and TMB methods but diffuse with DAB method.Under electron microscopy, there were electron-dense positive products in all the cells labeled either when alive or after death. In those cells labeled when alive, the products were found in the round or oval secondary lysosomes or multive-sicular bodies, while in those labeled after death, they were mostly found on one side of vacuoles without membrane boundary. In the fibers labeled after death, many large or small positive granules were found in the axons and many minute positive granules in the myelin lamellae especially on their inner surface in contact with the axons.

Objective To investigate the value of laparoscopic treatment for post-laparoscopic cholecystectomy complications.Methods From October 1996 to October 2007,totally 754 patients suffering from gallbladder diseases underwent laparoscopic cholecystectomy in our hospital;21 of them developed postoperative complications and received laparoscopic intra-abdominal exploration.Results By laparoscopy,bile leakage was diagnosed in 11 cases,intra-abdominal bleeding in 6,intraperitoneal fluid in 2,duodenal bulb perforation in 1,and obstructive jaundice in 1.The patients who developed obstructive jaundice was converted to open surgery because of hepatic duct transection.The other 20 cases underwent laparoscopic treatment.All the patients were followed up for a mean of 6 years(1 to 11),during which none of them had biliary complications.Conclusion Laparoscopic treatment is a microinvasive,safe and effective method for post-laparoscopic cholecystectomy complications.

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chemotherapy, Adjuvant ; Genomics ; Humans ; Neoadjuvant Therapy ; Neoplasm Staging ; Proteomics ; Quality of Life ; Salvage Therapy ; Stomach Neoplasms ; drug therapy ; pathology ; surgery ; Survival Rate

Communicable Diseases ; Disease Outbreaks ; Humans ; Infection Control ; Science

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Colonic Neoplasms ; drug therapy ; pathology ; Disease Progression ; Drug Administration Schedule ; Guidelines as Topic ; Humans ; Neoplasm Staging ; Survival Analysis

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chemotherapy, Adjuvant ; Colorectal Neoplasms ; radiotherapy ; surgery ; therapy ; Combined Modality Therapy ; Humans

Leukoaraiosis (LA) complicating stroke is more common.Studies have shown that LA is closely associated with the occurrence and prognosis of intracerebral hemorrhage.This article reviews the relationship between LA and primary intracerebral hemorrhage,intracerebral hemorrhage after anticoagulant and thrombolytic therapies as well as cerebral microbleeds.

T-DM1 is a novel antibody-drug conjugate that has similar biological activity with that of trastuzumab. T-DM1 specifically delivers DM1, the effective anti-microtubule drug, into the cytoplasm of tumor cells with HER2 overexpression. The efficacy of T-DM1 monotherapy is better than lapatinib in combination with capecitabine and T-DM and is expected to become the standard second-line treatment for HER2-positive advanced breast cancer drugs. Clinical trials that compare T-DM1 with trastuzumab joint taxane as the first-line of treatment for advanced breast cancer trials are currently being performed. T-DM1 is a brand new anti-HER2 drug after trastuzumab. U.S. FDA already approved T-DM1 as a drug for the treatment of HER2-positive advanced breast cancer patients.

Cholesterol is an essential nutrient for human body.Dietary cholesterol comes from animal sources,including eggs,pork and others.A number of epidemiological and experimental studies showed that too much intake of cholesterol or consumption of more than 1 egg per day would slightly elevate blood cholesterol level.Results of studies in different areas and populations suggested that there is a lack of sufficient evidence to support the association between cholesterol intakes or egg consumption and cardiovascular disease incidence or deaths.

98%. Conclusion The developed method is simple, reproducible and easy to operate. The solvent is cheap, with low boiling point and easy to recovery property.