Double negative T cells,namely DNT cells,are specific regulatory cells with TCR+ CD3 + CD4-CD8-in cell phenotype,divide into αβ T cell and γδ T cell,play a very important role in regulating immunoreaction.Previous research restricted to the treatment of autoimmune disease and organ transplantation immunity.Recently dnt cells adoptive immunotherapy,as a new anti-tumor method,gradually come into public view.This review mainly focuses on the source of DNT cells,their anti-tumor effect,as well as the possible mechanism.

Endometriosis is a common gynecologic disorder defined by the presence,growth,and invasion of endometrial tissue outside the uterine cavity.Although many hypotheses have been proposed,the etiology of the disease remains an enigma.Recent studies have suggested that factors within the peritoneal fluid contribute to endometriotic implant invasion,neovascularization,and proliferation.Chemokines,especially ENA-78,similar to IL-8,are postulated to play an essential role in the local inflammatory reactions seen in endometriosis.

Objective To investigate the human leucocyte antigen (HLA) expression of ectopic endometrial epithelial cells in cases with endometriosis and its role in pathogenesis of endometriosis Methods By immunohistochemical analysis,HLA expression was observed in ectopic endometrial epithelial cells of 25 endometriosis patients Ectopic endometrial cells were cultured in vitro successfully and the expression of HLA were analyzed by flow cytometry in 10 out of the 25 patients Eutopic endometrium of 15 normal women were taken as control Results (1)Expression of HLA class Ⅰ molecule on ectopic endometrial epithelial cells of endometriosis measured by the immunohistochemical analysis was lower than those of controls, (4 0?0 5) score and (1 2?0 8) score respectively So was the expression rate by flow cytometry in vitro culture (63 38?11 88)% and (5 27? 2 88)% respectively ( P

Recent studies have demonstrated that hyperhomocysteinemia is an independent risk factor for stroke,which may cause vascular endothelial injury by multiple mechanisms,destroy blood coagulation and fibrinolytic systems,and influence on lipid metabolism.Methylenetetra hydrofolate reductase(MTHFR)is a key enzyme of homocysteine metabolic pathway in the human body.If it is insufficient or its activity decreases,it will directly result in the accumula tion of homocysteine in the human body,and callse hyperhomocysteinemia.Although a number of studies have found that MTHFR gene mutation is the main cause of MTHFR insufficient and its activity decrease,the correlation between MTHFR gene mutation and ischemic cerebral vascular disease is quite controversial now.This article reviews the relationship between MTHFR gene polymorphisms and ischemic stroke.

ObjectiveTo study the characteristics of wedge factor of enhanced dynamic wedge (EDW) on Varian 600C linear accelerator . MethodsEnhanced dynamic wedge factors (EDWFs) of different jaw settings were measured on a Varian 600C linear accelerator with FARMER 2570 dosimeter and 2571 0.6 ml Thimble ion chamber. At the same time,equations were used to compute EDWFs of symmetric and asymmetric fields and off axis points. The same measurements were carried out on 30 degree physical wedge in contrast to EDW. The accelerator's jaw position was described according to IEC radiotherapy equipment coordinate standard. Results The EDWFs of symmetric field decreased smoothly while the field size increased. Under the same field,EDWFs decreased as the wedge angle increased and the increasing extent became apparent when the field became larger. In off axis fields formed by the upper independent jaws,only the EDWFs of 60 degree had the tendency to increase when the field center moved toward the toe of the wedge,the rest gave the opposite results. The EDWFs of fixed points varied only by 0.5 percent when the fixed jaw remained at the same place and the moving jaw and lower jaws stayed at different positions. But the WF of 30 degree physical wedge increased by 2.9 percent when the lower jaw settings increased from 4 cm to 40 cm. The EDWFs derived from analytic equations for symmetric and asymmetric fields coincided well with measured results. But at off-axis points,the differences between calculated values and measured data were apparent. ConclusionsThe curve of EDWF versus field size is very smooth. The EDWF of fixed point only depends on the position of the stationary upper jaw,and is affected little by the moving upper jaw and lower jaws. The EDWFs of off axis fields are much more complex than those of physical wedge; it shouldn't be confused in clinical application. The conversion method of rectangular field to square field is of no use when EDW field is confronted. Analytic methods are highly conformal with the calculation of EDWFs of symmetric and asymmetric fields.

Dyslipidemia is a well-established risk factor for atherosclerosis. Treating dyslipidemia in elderly patients requires specific knowledge and understanding of common dyslipidemias and the relative safety of various pharmacologic agents in the presence of possible multiple comorbidities. Lifestyle modification remains the first step in the treatment of dyslipidemia; however, it can be difficult to sustain and achieve acceptable compliance in the elderly and it is best used in combination with drug therapy. Statins are widely accepted as the first-line therapy. Several recent studies have demonstrated that statins are safe and effective in the elderly. However, it is important to note that there is very limited data regarding the effects of dyslipidemia treatment on morbidity and mortality in patients over 85 years of age. In summary, the clinicians must recognize that the presence of dyslipidemia in the elderly poses substantial risk of coronary events and stroke. The available evidence has demonstrated that in most elderly patients who are at increased risk for cardiovascular morbidity and mortality, treatment of dyslipidemia with appropriate therapy reduces the risk, and when used carefully with close monitoring for safety, the treatment is generally well tolerated. With increasing life expectancy, it is critical for physicians to recognize the importance of detection and treatment of dyslipidemia in the elderly.

Factors in uterus may be important in the aetiology of type 2 diabetes.Malnutrition during early life can affect insulin secretion and result in insulin resistance,and such effects are markedly exaggerated when facing over-nutrition in later life.This paper gives a review about the population evidence and epigenetic mechanism in the generational susceptibility to type 2 diabetes.

Objective:To observe the expression of Clusterin in colorectal cancer tissues and study its relationship with the development and progression of colorectal cancer.Methods:Fifty-eight colorectal cancer tissues samples(32 rectal cancer tissues,26 colon carcinoma tissues) of patients with completed clinic data were obtained after surgery from the Affiliated Hospital of Guangdong Medical College.Immunohistochemical staining(SP method) was used to detect the expression of Clusterin in colorectal cancer tissue,adjacent normal tissue of colorectal cancer and normal tissue.Flow cytometry was used to examine the apoptosis of colorectal cancer cells.Results:The expression of Clusterin in colorectal cancer tissues was significantly higher than those in the adjacent tissues of colorectal cancer and normal colorectal tissue(P

After the health information service for patients in hospitals was described,its characteristics,service process and management model were elaborated,which showed that the clear and reliable sources of hospital forum-provided medical and health information could serve the patients more effectively.

On the model of isoprenaline (ISO)-perinjured rat heart, the protctive effect of ischemic preconditioning (IPC) on ischemia reperfusion (I/R) damage was observed.It was found that compared with alone I/R group,IPC ameliorated I/R-induced reduced reduction of coronary blood flow (CBF) (8. 8 + 0. 6 vs 6. 6 + 0. 4mL/min,P