Objective To study the expression of SARS-associated coronavirus nucleocapsid(N) gene in Pichia pastoris and to obtain recombinant N protein with good biological activity.Methods The gene encoding the full N protein of SARS-CoV was amplified by PCR and cloned into Pichia pastoris expression vector pPICZA.The recombinant expression plasmid pPICZA/N was transformed into X-33,KM71H and GS115. The positive insert transformants were screened,cultured and induced by methanol.The recombinant protein was further purified with Ni affinity chromatography.Antigen activity was detected with anti-N monoclonal antibody,polyclonal antibody and sera from SRAS patients.Results SDS-PAGE and immunological analysis demonstrated that only Pichia pastoris transformants KM71H/pPICZA/N and X-33/pPICZA/N expressed Mr 70 000 fusion protein with special antigenicity.Conclusion SARS-CoV N protein expression in Pichia pastoris has been achieved,establishing the basis for further study of biological and immunological function of N antigen.

Objective:To explore improving effect of Danqi soft capsule on hemorheology in patients with coronary heart disease (CHD) and its myocardial protection effect .Methods:A total of 84 CHD patients treated in our hos‐pital from Dec 2013 to Dec 2014 were enrolled .They were randomly divided into routine treatment group (n=42) and combined treatment group (n=42 ,received Danqi soft capsule therapy based on routine treatment group ) .He‐morheological indexes ,ECG and inflammatory factor levels [C reactive protein (CRP) ,tumor necrosis factor α(TNF‐α)] were measured and compared between two groups before and after treatment .Results:Compared with before treatment , above hemorheological indexes significantly reduced in both groups after treatment ( P< 0.05 all) ,compared with routine treatment group ,there were significant reductions in high shear whole blood viscosity [ (5.12 ± 0.73) mPa/s vs .(4.47 ± 0.86) mPa/s] ,low shear whole blood viscosity [ (7.36 ± 1.32) mPa/s vs .(6.21 ± 1.03) mPa/s] ,plasma viscosity [ (1.47 ± 0.31) mPa/s vs .(1.31 ± 0.25) mPa/s] ,erythrocyte aggregation index [ (3.53 ± 0.45) vs .(2.93 ± 0.26)] and fibrinogen level [ (2.95 ± 0.28) g/L vs .(2.08 ± 0.24) g/L] in combined treatment group , P< 0.05 all .Total effective rate on ECG in combined treatment group was significantly higher than that of routine treatment group (85.71% vs .57.14% ,P<0.05) .Compared with before treatment ,CRP and TNF‐αlevels significantly reduced in both groups after treatment ( P<0.05 all) ,compared with routine treatment group ,there were significant reductions in levels of CRP [ (6.52 ± 1.61) mg/L vs .(5.18 ± 1.25) mg/L] and TNF‐α [ (283.28 ± 18.28) pg/ml vs .(245.08 ± 12.24) pg/ml] in combined treatment group , P<0.05 all .Conclusion:Danqi soft capsule combined routine treatment can significantly improve hemorheology and reduce related inflamma‐tory factor levels ,possess myocardial protection effect in CHD patients ,which is worth extending .

Communicable Diseases ; Disease Outbreaks ; Humans ; Infection Control ; Science

Objective To study the nursing methods of gastrointestinal cancer patients undergoing continuous hyperthermic peritoneal peffusion chemotherapy (CHPPC) in the peri-operative period.Methods A total of 126 patients with gastrointestinal cancer underwent continuous hyperthermic peritoneal perfusion chemotherapy,and nursing care was implemented continuously,comprehensively and individually.Results All of the patients underwent continuous hyperthermic peritoneal perfusion chemotherapy successfully,without any complication occurred.Conclusions Intensive and effective nursing care is helpful to carry out continuous hyperthermic peritoneal perfusion chemotherapy safely,and avoid the complications.

Objective To explore the technique of laparoscopic reoperation in the treatment of common bile duct stones.Methods A retrospective analysis of the clinical data of 54 cases of common bile duct stones undergoing laparoscopic common bile duct stone surgery in our hospital from January 2011 to December 2015 was conducted.Through the choice of puncture hole, the exposure of the liver surface and the confirmation of the common bile duct, T-tube drainage and primary suture of common bile duct were carried out for the treatment of bile duct stone.Results The laparoscopic operations were successful in 52 cases, while conversions to open surgery were required in 2 cases.During the operation, the amount of bleeding was about 40-150 ml (average, 70 ml), the operation time was 75-180 min (average, 120 min), and the postoperative gastrointestinal function recovery time was 24-48 h (average, 27±13 h).There were 1 case of duodenal perforation injury, 2 cases of bile leakage, and 2 cases of residual stones after operation, which were cured with laparoscopic repair, prolonged drainage time, and choledochoscope treatment via abdominal sinus.Postoperative hospital stay was 6-9 d (average, 7±1.5 d).A total of 54 cases were followed up for 3-24 months (mean, 14.8 months).All the patients recovered well, without bile duct stricture or stone recurrence.Conclusion As long as the application of skilled laparoscopic surgery methods and separation of surgical area skills, laparoscopic bile duct surgery for the treatment of common bile duct stones is safe and feasible.

Objective To observe MSCT features of partially or completely low enhancement of pancreas neuroendocrine tumors (PNENs).Methods The clinical data and MSCT features before pancreatectomy in 44 patients with confirmed PNENs were retrospectively reviewed.The MSCT findings were evaluated including tumor size, location, margin, density, intratumoral structure, bile duct and pancreatic ductal dilation and distant metastasis.Tumors were classified into complete enhancement type, partially or completely low enhancement type for further comparative analysis based on MSCT enhancement during pancreatic stage.Results A total of 56 PNENs in 44 patients were found, and there were 31 partially or completely low enhanced PNENs and 25 completely enhanced PNENs.The former were larger than the latter [mean tumor size, (3.3±2.2)cm vs (1.4±0.9) cm], and irregular shape and cystic components within tumors were more often observed (all P<0.05).There were no significant differences between the two types of PNENs in terms of gender, the presence of functional tumor, tumor location, clear tumor margin, intratumoral calcification, bile and pancreatic duct dilation and metastasis.76.0%(19/25) of completely enhanced PNENs reached peak enhancement in arterial phase, and 71.0%(22/31) of low enhancement PNENs reached peak in pancreatic phase.Enhanced intratumoral blood vessels in the arterial phase were more frequent in low enhancement PNENs, and the difference was statistically significant (P<0.05).There were significant differences on pathological grade between the two types of PNENs (G1=21,G2=4,G3=0 vs G1=18,G2=5, G3=8), and the difference was statistically significant (P<0.05).Conclusions Compared with complete enhancement PNENs, partially or completely low enhancement PNENs had bigger size, irregular shape, and cystic component.Intratumoral blood vessels in the arterial phase were observed, peak enhancement arrived later and the pathological grade was higher.

General Policies of Nomenclature for Biological Products in China were studied,aimed at establishing and perfecting the system of Chinese approved biologic name.By means of analysis of the current situation and existing problems of nomenclature for biological products and the development trend of new biotherapeutics.It is sufficiently studied on the existing leading international drug generic naming system,and the specific suggestions on revision of the General Policies of Nomenclature for Biological Products in China based on the naming principles of WHO INN and Chinese language recognition were put forward.

Objective To provide an information monitoring software for therapy drug,which can automatically construct safe concentration scope,statistically analyses data,query and input data.Methods TDMIS is developed by Powerbuilder 9.0 and is run in WIN 98 or the copy over it.2062 cases are analyzed through TDMIS and a safe concentration scope is set.Conclusion TDMIS is a practical software.It can make drug prescription standardized,computerized and easy to be statistically analyzed.The working efficiency and quality of clinical apothecary are greatly improved.

Objective To evaluate the difference between gallbladder polys,gallbladder adenomas and gallbladder cancer by sonography.Methods The imaging features were analysed including 571 patients with gallbladder polys,30 with gallbladder adenomas,109 with gallbladder cancer.Results The lesion diameter,echogram,color signals,maximum velocity(Vmax) and the resistive index(RI) were difference between Polys,Adenomas and cancers of gallbladder.When RI withinlesions lower than 0.65 was adopted as diagnostic criteria of gallbladder cancer,the specificity of the ultra-sound in diagnosis of gallbladder cancer were respectively.Conclusion Color Doppler flow imaging was useful in the differential diagnosis of gallbladder polypoid lesions in combination with gray-scale sonography.

Objective To observe the effects of urine on TGF-?1 and Smad3 expression in cultured human urethral fibroblasts and explore the mechanism of urethral scar fibrosis.Methods The human urethral fibroblasts were cultured and stimulated by urine or 350 ?mol/L uric acid.The mRNA and protein expression of TGF-?1 and Smad3 were measured by PT-PCR and immunofluorescent staining,respectively.Collagen Ⅰlevels in the supernatants in different groups were detected by ELISA.Results TGF-?1 and Smad3 were detected in human urethral fibroblasts by immunofluorescent staining.After being presented with urine or uric acid at physiological concentration,both TGF-?1 and Smad3 in human urethral fibroblasts were increased significantly at both the protein and mRNA levels(P