Objective:To explore improving effect of Danqi soft capsule on hemorheology in patients with coronary heart disease (CHD) and its myocardial protection effect .Methods:A total of 84 CHD patients treated in our hos‐pital from Dec 2013 to Dec 2014 were enrolled .They were randomly divided into routine treatment group (n=42) and combined treatment group (n=42 ,received Danqi soft capsule therapy based on routine treatment group ) .He‐morheological indexes ,ECG and inflammatory factor levels [C reactive protein (CRP) ,tumor necrosis factor α(TNF‐α)] were measured and compared between two groups before and after treatment .Results:Compared with before treatment , above hemorheological indexes significantly reduced in both groups after treatment ( P< 0.05 all) ,compared with routine treatment group ,there were significant reductions in high shear whole blood viscosity [ (5.12 ± 0.73) mPa/s vs .(4.47 ± 0.86) mPa/s] ,low shear whole blood viscosity [ (7.36 ± 1.32) mPa/s vs .(6.21 ± 1.03) mPa/s] ,plasma viscosity [ (1.47 ± 0.31) mPa/s vs .(1.31 ± 0.25) mPa/s] ,erythrocyte aggregation index [ (3.53 ± 0.45) vs .(2.93 ± 0.26)] and fibrinogen level [ (2.95 ± 0.28) g/L vs .(2.08 ± 0.24) g/L] in combined treatment group , P< 0.05 all .Total effective rate on ECG in combined treatment group was significantly higher than that of routine treatment group (85.71% vs .57.14% ,P<0.05) .Compared with before treatment ,CRP and TNF‐αlevels significantly reduced in both groups after treatment ( P<0.05 all) ,compared with routine treatment group ,there were significant reductions in levels of CRP [ (6.52 ± 1.61) mg/L vs .(5.18 ± 1.25) mg/L] and TNF‐α [ (283.28 ± 18.28) pg/ml vs .(245.08 ± 12.24) pg/ml] in combined treatment group , P<0.05 all .Conclusion:Danqi soft capsule combined routine treatment can significantly improve hemorheology and reduce related inflamma‐tory factor levels ,possess myocardial protection effect in CHD patients ,which is worth extending .

Objective To study the expression of SARS-associated coronavirus nucleocapsid(N) gene in Pichia pastoris and to obtain recombinant N protein with good biological activity.Methods The gene encoding the full N protein of SARS-CoV was amplified by PCR and cloned into Pichia pastoris expression vector pPICZA.The recombinant expression plasmid pPICZA/N was transformed into X-33,KM71H and GS115. The positive insert transformants were screened,cultured and induced by methanol.The recombinant protein was further purified with Ni affinity chromatography.Antigen activity was detected with anti-N monoclonal antibody,polyclonal antibody and sera from SRAS patients.Results SDS-PAGE and immunological analysis demonstrated that only Pichia pastoris transformants KM71H/pPICZA/N and X-33/pPICZA/N expressed Mr 70 000 fusion protein with special antigenicity.Conclusion SARS-CoV N protein expression in Pichia pastoris has been achieved,establishing the basis for further study of biological and immunological function of N antigen.

Communicable Diseases ; Disease Outbreaks ; Humans ; Infection Control ; Science

Objective To study the nursing methods of gastrointestinal cancer patients undergoing continuous hyperthermic peritoneal peffusion chemotherapy (CHPPC) in the peri-operative period.Methods A total of 126 patients with gastrointestinal cancer underwent continuous hyperthermic peritoneal perfusion chemotherapy,and nursing care was implemented continuously,comprehensively and individually.Results All of the patients underwent continuous hyperthermic peritoneal perfusion chemotherapy successfully,without any complication occurred.Conclusions Intensive and effective nursing care is helpful to carry out continuous hyperthermic peritoneal perfusion chemotherapy safely,and avoid the complications.

Objective To explore the decision-making processing changes of patients with cerebral infarction in different regions.Methods The patients with cerebral infarction were divided into 21 cases of frontal lobe infarction,11 cases of temporal lobe infarction,19 cases of the medial temporal lobe infarction,25 cases of basal ganglia infarction;25 cases of the anterior infarction,51 cases of the posterior infarction,62 cases of depression,64 cases of anxiety ;and there were 125 cases in normal control group.All subjects completed the test of the six kinds of choice situational problems,and used MMSE to evaluate cognitive function,the Hamilton Depression and anxiety Scale Evaluation to evaluate emotion.Results All cerebral infarction patients (CI group) and normal control group in low-risk and no-risk of loss situation(choice scenario 2),high-risk and the no-risk of gain or loss situation(choice scenario 3 and 4),high-risk and low-risk of gain or loss situation(choice scenario 5 and 6) tended to choose conservative scheme,and the selection probability of conservative scheme were obviously higher than that of the normal group,and the differences were statistically significant (P ＜ 0.05).In choice scenario 3,the basal ganglia infarction group with frontal lobe infarction group,the medial temporal lobe infarction group comparison,the conservative income scheme selection probability increased significantly,all difference were statistically significant (P ＜ 0.01,P ＜ 0.05).In choice scenario 5 of the posterior infarction group,a higher probability of conservative income scheme was selected than the anterior infarction,and the difference was statistically significant (P ＜0.05).Accompanied with depression and anxiety,the basal ganglia infarction tended to select more conservative income program than the frontal lobe infarction group and the medial temporal lobe infarction group in choice scenario 3,and the program infarction group selected a higher probability comparative differences were statistically significant (P＜ 0.01,P＜ 0.05).The posterior infarction group selected a higher probability of conservative income scheme than the anterior infarction in choice scenario 5,and the difference was statistically significant (P ＜ 0.05).Conclusion Cerebral infarction in patients exist decision-making processing abnormally,and perform as income conservative and loss risk averse.Whether associated with depression,anxiety,the basal ganglia infarction and posterior cerebral infarction patients are inclined to income conservative.

This paper studied the usage and impact of the theory in hospital development.Based on such,special manifestations behind the marginal diminishing effect are taken into consideration to grasp particular demands and requirements of health,and to look for a positive role and a progressive increase of the marginal utility.Furthermore,the explorations in hospitals,such as technical innovations,structure optimizations and management breakthroughs,as well as the experimental and practical construction of micro-economic administrative mechanism in clinical departments were discussed in the text.It has been demonstrated in practice that the practice has a significant effect on the prevention of marginal diminishing utility and an essential reference on hospital transformational development.

Objective In order to explore the mechanism of repetitive transcranial magnetic stimulation rTMS) on neuropathic pain,we observed the effect of different frequencies of rTMS on neuropathic pain and the expression of neuronal nitric oxide synthase (nNOS) in the dorsal root ganglion (DRG).Methods A total of 28 male Sprague-Dawley rats were used and divided into a control group,in which sham-operation was performed,and an experimental group which was further divided into a sham-rTMS group,a 1 Hz group and a 20 Hz group after successful neuropathic pain model was established by operation to ligate the left sciatic nerves,with 7 rats in each group.Three days after the operation,rTMS was applied to the primary motor cortex (M1) contralateral to the pain once dai ly for 10 consecutive days.The pain behavior and nNOS expression in the DRG were measured before and after 10 days of rTMS intervention.Results All the neuropathic pain model rats demonstrated pain-related behaviors 3 days postoperation,the mechanical pain thresholds were significantly lower than those in the control group (P ＜ 0.05).After rTMS treatment,the mechanical hyperalgsia was significantly relieved in 20 Hz group but not 1 Hz group as demonstrated by a comparison with the sham-rTMS group (P ＜ 0.05).The expression of nNOS in DRG ipsilateral to the neuropathic pain was significantly increased in sham-rTMS group and 1 Hz group (P ＜ 0.05) when compared with the control group.Meanwhile,it was shown that expression of nNOS was down-regulated in 20 Hz group but not 1 Hz group (P ＜ 0.05).The degree of pain relief in 20 Hz group was negatively correlated with the expression of nNOS in DRG (P ＜0.05).Conclusions Neuropathic pain induced by peripheral nerve injury is associated with elevated expression of nNOS in the DRG.High-frequency rTMS can relieve neuropathic pain through down-regulating the overexpression of nNOS in the DRG,but the low-frequency rTMS has no such effect.

Objective To investigate the value of multi-slice spiral CT(MSCT)in diagnosis of the causes of intestinal obstruction.Methods 49 cases of intestinal obstruction confirmed by surgery or clinical diagnosis underwent abdominal MSCT plain and enhanced scanning,three-dimensional post-processing reconstruction was done in 5 cases.CT features were analysed blindly by two senior radiologists,including the thickness,density and degree of enhancement of intestinal walls at the "transition zone" area of intestine and surrounding mesenteric structure.The causes of intestinal obstruction were analysed in combination with clinical history of the patients.Results The causes of intestinal obstruction were suggested by MSCT in 46 cases(94%),including intestinal adhesion in 13 cases,tumor in 11,hernia in 4,ischemic bowel disease in 4,gallstone in 2,gastrolith in 2,Bezoar in 4.paralytic ileus in 3 and appendiceal abscess in 3.Three cases were misdiagnosed.Conclusion MSCT is of significant clinical value in diagnosis of intestinal obstruction,which should be used as the first means in clinic practice.

Pancreatic cancer is a common malignant neoplasm of the pancreas with an extremely high mortality.Currently,the early diagno-sis of pancreatic cancer is still not ideal.Attention should be paid to some clinical warning symptoms,such as unexplained abdominal and back pain,jaundice,and unexpected diabetes.Additionally,the combined use of CA19-9,CEA,and other tumor markers,the attention to biochemical indicators,the detection of mutation in KAI1 or p53 gene,and the exploration of the value of miRNA in clinical diagnosis are of great significance.On the other hand,ultrasound,CT,MRCP,ERCP,PET-CT,and other imaging methods,as well as effective col-lection of cytology specimens,should be performed.Thus,there is hope for the early diagnosis of pancreatic cancer.

Objective To observe the effects of repeated low and high-frequency transcranial magnetic stimulation (rTMS) on neuropathic pain and the expression of specific activation markers of astrocytes and glial fibrillary acidic protein (GFAP) in the lumbar spinal cord.Methods Twenty-eight male Sprague-Dawley rats were randomly divided into a sham-operated group,a sham-rTMS group,a 1 Hz group and a 20 Hz group,with 7 in each group.The rats in the sham-operated group had their sciatic nerve exposed without ligation,while the other groups underwent sciatic nerve ligation to induce neuropathic pain.Three days after the operation,rTMS was applied to the primary motor cortex (M1) contralateral to the pain once a day for 10 consecutive days.Pain-related behavior and thermal pain response were tested before the operation and before and after the course of rTMS therapy.The expression of GFAP in the lumbar spinal cord was examined.Results All of the rats which underwent sciatic nerve ligation showed pain-related behavior and significantly decreasing thermal pain latency compared with the sham-operated group.After the rTMS therapy the thermal hyperalgia was significantly attenuated in the 20 Hz group but not in the 1 Hz group compared with the sham-rTMS group.The expression of GFAP in the dorsal horn of the lumbar spinal cord ipsilateral to the neuropathic pain was significantly increased in the sham-rTMS group and the 1 Hz group compared with the sham-operated group.Compared with the sham-rTMS group,GFAP levels were significantly lower in the 20 Hz group but not in the 1 Hz group.The pain relief in the 20 Hz group was negatively related to the expression of GFAP.Conclusions Neuropathic pain induced by peripheral nerve injury is associated with increased activity and proliferation of astrocytes in the dorsal horns of the spinal cord.High-frequency rTMS can relieve neuropathic pain through inhibiting the activity and proliferation of astrocytes in the dorsal horns,but low-frequency rTMS has no clinically significant effect.