Objective To study the expression of SARS-associated coronavirus nucleocapsid(N) gene in Pichia pastoris and to obtain recombinant N protein with good biological activity.Methods The gene encoding the full N protein of SARS-CoV was amplified by PCR and cloned into Pichia pastoris expression vector pPICZA.The recombinant expression plasmid pPICZA/N was transformed into X-33,KM71H and GS115. The positive insert transformants were screened,cultured and induced by methanol.The recombinant protein was further purified with Ni affinity chromatography.Antigen activity was detected with anti-N monoclonal antibody,polyclonal antibody and sera from SRAS patients.Results SDS-PAGE and immunological analysis demonstrated that only Pichia pastoris transformants KM71H/pPICZA/N and X-33/pPICZA/N expressed Mr 70 000 fusion protein with special antigenicity.Conclusion SARS-CoV N protein expression in Pichia pastoris has been achieved,establishing the basis for further study of biological and immunological function of N antigen.

Objective:To explore improving effect of Danqi soft capsule on hemorheology in patients with coronary heart disease (CHD) and its myocardial protection effect .Methods:A total of 84 CHD patients treated in our hos‐pital from Dec 2013 to Dec 2014 were enrolled .They were randomly divided into routine treatment group (n=42) and combined treatment group (n=42 ,received Danqi soft capsule therapy based on routine treatment group ) .He‐morheological indexes ,ECG and inflammatory factor levels [C reactive protein (CRP) ,tumor necrosis factor α(TNF‐α)] were measured and compared between two groups before and after treatment .Results:Compared with before treatment , above hemorheological indexes significantly reduced in both groups after treatment ( P< 0.05 all) ,compared with routine treatment group ,there were significant reductions in high shear whole blood viscosity [ (5.12 ± 0.73) mPa/s vs .(4.47 ± 0.86) mPa/s] ,low shear whole blood viscosity [ (7.36 ± 1.32) mPa/s vs .(6.21 ± 1.03) mPa/s] ,plasma viscosity [ (1.47 ± 0.31) mPa/s vs .(1.31 ± 0.25) mPa/s] ,erythrocyte aggregation index [ (3.53 ± 0.45) vs .(2.93 ± 0.26)] and fibrinogen level [ (2.95 ± 0.28) g/L vs .(2.08 ± 0.24) g/L] in combined treatment group , P< 0.05 all .Total effective rate on ECG in combined treatment group was significantly higher than that of routine treatment group (85.71% vs .57.14% ,P<0.05) .Compared with before treatment ,CRP and TNF‐αlevels significantly reduced in both groups after treatment ( P<0.05 all) ,compared with routine treatment group ,there were significant reductions in levels of CRP [ (6.52 ± 1.61) mg/L vs .(5.18 ± 1.25) mg/L] and TNF‐α [ (283.28 ± 18.28) pg/ml vs .(245.08 ± 12.24) pg/ml] in combined treatment group , P<0.05 all .Conclusion:Danqi soft capsule combined routine treatment can significantly improve hemorheology and reduce related inflamma‐tory factor levels ,possess myocardial protection effect in CHD patients ,which is worth extending .

Objective To provide an information monitoring software for therapy drug,which can automatically construct safe concentration scope,statistically analyses data,query and input data.Methods TDMIS is developed by Powerbuilder 9.0 and is run in WIN 98 or the copy over it.2062 cases are analyzed through TDMIS and a safe concentration scope is set.Conclusion TDMIS is a practical software.It can make drug prescription standardized,computerized and easy to be statistically analyzed.The working efficiency and quality of clinical apothecary are greatly improved.

Objective To evaluate the difference between gallbladder polys,gallbladder adenomas and gallbladder cancer by sonography.Methods The imaging features were analysed including 571 patients with gallbladder polys,30 with gallbladder adenomas,109 with gallbladder cancer.Results The lesion diameter,echogram,color signals,maximum velocity(Vmax) and the resistive index(RI) were difference between Polys,Adenomas and cancers of gallbladder.When RI withinlesions lower than 0.65 was adopted as diagnostic criteria of gallbladder cancer,the specificity of the ultra-sound in diagnosis of gallbladder cancer were respectively.Conclusion Color Doppler flow imaging was useful in the differential diagnosis of gallbladder polypoid lesions in combination with gray-scale sonography.

Objective To observe the effects of urine on TGF-?1 and Smad3 expression in cultured human urethral fibroblasts and explore the mechanism of urethral scar fibrosis.Methods The human urethral fibroblasts were cultured and stimulated by urine or 350 ?mol/L uric acid.The mRNA and protein expression of TGF-?1 and Smad3 were measured by PT-PCR and immunofluorescent staining,respectively.Collagen Ⅰlevels in the supernatants in different groups were detected by ELISA.Results TGF-?1 and Smad3 were detected in human urethral fibroblasts by immunofluorescent staining.After being presented with urine or uric acid at physiological concentration,both TGF-?1 and Smad3 in human urethral fibroblasts were increased significantly at both the protein and mRNA levels(P

Objective To explore the treatment approach to severe l ower extremity trauma. Methods A retrospective study was ca rried out in 42 cases with severe lower extremity trauma from 1989 to 1999. Mang led extremity severity score (MESS) was 6.24?1.45 in all the cases, among which were 34 cases with MESS

OBJECTIVE:To investigate the hemostatic effect and safety of hemocoagulase in radical gastrectomy.METHODS:60 cases with gastric cancer were divided on average into 2 groups.Therapy group received hemocoagulase 2 KU during and after operation,and control group received dicynone 0.5 g and aminomethylbenzoic acid 0.2 g during and after operation.Intraoperative blood loss and amount of blood drainage at 6 h and 24 h after operation were observed.The value of D-dimer was monitored after operation.RESULTS:Intraoperative blood loss and amount of blood drainage at 6 h and 24 h after operation in therapy group were less than in control group (P

Long non-coding RNAs ( lncRNAs) are a class of RNA molecules which are unable to codify proteins. They express abnormally in tumor cells including prostate cancer cells and play a significant role in development of tumor. Some lncRNAs have been proven to affect physiological processes of prostate cells and act as tumor suppressor or activator and could be regulated through several methods. Thus, lncRNAs have the potential to be therapeutic targets of prostate cancer. This review introduces expressed abnormally ln-cRNAs in prostate cancer and their functions and mechanisms in prostate cancer.

Pancreatic cancer is a common malignant neoplasm of the pancreas with an extremely high mortality.Currently,the early diagno-sis of pancreatic cancer is still not ideal.Attention should be paid to some clinical warning symptoms,such as unexplained abdominal and back pain,jaundice,and unexpected diabetes.Additionally,the combined use of CA19-9,CEA,and other tumor markers,the attention to biochemical indicators,the detection of mutation in KAI1 or p53 gene,and the exploration of the value of miRNA in clinical diagnosis are of great significance.On the other hand,ultrasound,CT,MRCP,ERCP,PET-CT,and other imaging methods,as well as effective col-lection of cytology specimens,should be performed.Thus,there is hope for the early diagnosis of pancreatic cancer.

Left atrial volume index (LAVI) is related to systolic and diastolic function of heart .It can reflect the se‐verity of left atrial dilation and heart failure (HF) ,and is a powerful index predicting poor prognosis of HF .This article made a review on the relationship among cardiac function ,prognosis and LAVI in HF patients ,and the diag‐nostic value of LAVI for HF patients .