Objective:To pediatric patients with scalp venous indwelling needle infusion therapy, and special care for children, and analyze its clinical effect. Methods:from 2011 February to 2012 August in our hospital outpatient department of pediatrics infusion in 180 cases, All the children were using scalp venous indwelling needle infusion therapy, and special care for children, and observe its clinical index. Results:after the treatment, the disposable puncture success rate was 93.3%, the blood pollution rate is 5.6%, the injection time (P<0.01) and before use, contrast, statistically significant differences. Conclusion:infusion of pediatric patients with scalp superficial vein indwelling, in clinical application is safe and reliable, convenient and feasible, but also can reduce the pain of patients, reduce the workload of nursing staff, is worthy of clinical use.

In light of the idea of realizing management by capital, the authors expound the theoretical basis of implementing the integration of the capital of funds and the capital of knowledge and give an account of their hospital's experience in this aspect. By introducing capital and talented personnel, viz. by liquidating remnant assets through the capital of knowledge and by seeking benefits through capital integration, the hospital has succeeded in inventing a new way of utilizing talented personnel resources and stimulating talented personnel to greater efforts, thus enhancing the speed of its accumulation of capital and laying a solid foundation for sustainable development.

In light of their hospital's practice in adopting new operational ideas and management models for the past three years, the authors expound the content of the new operational ideas, the necessity of applying these new ideas to hospital management, and the four basic principles that must be followed in constructing new management models. They also discuss some key issues involved in the process of the hospital's construction of new management models.

Problem-based learning(PBL) teaching mode was applied in Urology practice of the medical students.The externs were organized to analyze and discuss a real case,having grasped enough knowledge of relative diseases,and to provide their advice about the diagnosis and treatment,changing question mark into a full stop.And then their conclusions were verified by post-operational follow-up.PBL teaching mode can increase the students'study motivations and interests and the ability to resolve practical problems,master medical knowledge,train their clinical thinking and enhance the quality of teaching and thus it is an effective teaching mode.

Objective:To investigate the inhibitory effect of antisense oligodeoxynucleotide (ASODN) targeting survivin gene on gastric cancer cell line SGC-7901. Methods: Cultured SGC-7901 cells were divided into 6 groups: control group, lipofectin group, oligodeoxynucleotide(ODN) group, 1?10-5mol/L ASODN group, 3?10-5mol/L ASODN group and 5?10-5mol/L ASODN group. After incubated with ASODN, the expression of survivin mRNA in the SGC-7901 cells was examinated by PCR; the apoptosis of SGC-7901 cells was studied by the morphological observation using inverted microscope, electron microscope, flow cytometry and DNA agarose electrophoresis; telomerase activity was determined using PCR-based TRAP (telomere repeat amplification protocol) assay; MTT assay was used to study the inhibitory effect of ASODN on the proliferation of SGC-7901 cells. Results: ASODN obviously inhibited the expression of survivin mRNA in the SGC-7901 cells. The apoptotic rate of gastric cell was significantly increased after incubated with ASODN ([7.01?0.21]% vs [25.0?0.52]%, P

A great progress has been achieved in research on repair of nerve functional recovery following spinal cord injury because of the development of nerve tissue engineering.As an important part of nerve tissue engineering,cell scaffold is divided into natural biomaterials,synthetic materials and composite materials.Besides,nanotechnology has been applied to study scaffolds.However,there are many problems in the application of cell scaffold and further study is needed.With the continued development of biotechnique and cross-correlated technique,the cell transplantation scaffold will make more effect on repair of spinal cord injury.

Objective To investigate the influence of heat and moisture exchanger filter(HMEF)on the temperature of inhaled and endotracheal gas during mechanical ventilation. Methods Thirty patients (ASAI-II) were selected and divided randomly into 2 groups: control group and HMEF group. The temperature of inhaled gas was monitored with hygrothermograph and the temperature of endotracheal gas was monitored with temperature probe. Baseline values were set at the moment of intubation and then the values were recorded at the first hour after intubation and the second hour after intubation. Results With the increase of the mechanical ventilation time, the temperature of inhaled and endotracheal gas decreased significantly in control group and increased significantly in HMEF group; The temperature of both inhaled and endotracheal gas in HMEF group were significantly higher than that in control group at the moment of the first hour after intuba- tion and the second hour after intubation; There was correlation between the temperature of inhaled gas and the temperature of endotracheal gas at the moment of intubation, but it was not the same at the moment of the first hour after intubation or the second hour after intubation. Conclusion HMEF can make the cold inhaled gas warmer when it reached the trachea; Temperature probe may be better than hygrothermograph for monitoring the temperature of trachea.

Erythrocyte aggregation and related parameters were observed in this study.Erythrocyte aggregation index, hematocrit and fibrin fibrinogen degradation products in-creased significantly at 5 minutes after oleic acid injection. Erythrocyte electrophoretic timein native plasma delayed obviously at 5 minutes after oleic acid injection, whereas that inPBS altered very little. The concentration of plasma calcium and erythrocyte intracellularmagnesium decreased at 5 minutes, while plasma magnesium increased. The stepwise reg-ression analysis suggested that during lung injury the parameters that have greatest effect onerythrocyte aggregation were the change of hematocrit, erythrocyte intracellular magnesiumand negative charge of erythrocyte surface.

Objective To investigate the technique and effectiveness of CT-guided collagenase injection into prominence of herniated lumbar disc and the surface of prominence in the treatment of lumbar disc herniation. Methods One hundred and fifty-five patients with lumbar disc herniation diagnosed by clinic and CT received CT-guided collagenase injection, with the approaches via intervertebral foramen in L3-4 and L4-5, and the approaches via margo medialis of joint processus articularis in L5-S1. Results Injection was done successfully in all patients (100%), the clinical rate of excellent and good results were 94.2%. The herniation disappeared or significantly reduced as demonstrated by CT scan in 32 of 34 patients (94.1%), the rate of excellent and good results were 100%. No changes existed in 2 cases. There were 2 cases of disc inflammation in our group. Conclusions CT-guided collagenase injection into the prominence of herniated lumbar disc and its surface is a safe and efficient method in the treatment of herniated lumbar disc.

Peripheral nerve block technique is one of the basic skills necessary to residents and it is also one of the difficult points in the resident standardized training in the department of anesthesiology. In order to maximize the learning effect of the anesthesia professional residents in the case of limited number of cases, we need to constantly enrich the teaching technologies and teaching methods. Human 3D anatomy is used for theory teaching to maintain comprehension of nerve anatomy; Ultrasound-guided nerve block technique and ultrasound simulation training are used for nerve block practice to improve nerve block skills;Problem-based learning teaching method is introduced in nerve block cases to increase the residents' ability of clinical cases management.