1.Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis adult worms by Western blot
Xu, D.M. ; Wen, H. ; Wang ; L.A., Hu ; C.X., Qi ; X., Sun ; G.G., Liu, R.D. ; Wang, Z.Q. ; Cui, J.
Tropical Biomedicine 2017;34(1):191-198
Previous studies showed that crude antigens from Trichinella spiralis adult worms
(AW) can be recognized by mouse infection sera at 8 days post infection. The aim of this study
was to identify the early diagnostic antigenic bands in soluble proteins from T. spiralis AW by
Western blot using early infection sera. The affecting factors of adult recovery were firstly
observed in this study, and the results showed that the maximum number of adults was
collected from small intestine when the female BALB/c mice were orally infected with 4000
ML and sacrificed at 3 days post infection. The results of Western blot analysis showed that
seven protein bands (31, 35.1, 39, 40.6, 41.9, 47 and 50.6 kDa) could be recognized by early
infection sera as early as at 8-10 days post infection, and were strongly reacted with mouse
infection sera at 11-12 days post infection. Our results suggested that the seven protein
bands of T. spiralis AW soluble proteins might be the early expressed antigens during the
intestinal stage of Trichinella infection and therefore have potential value for the early
diagnosis of trichinellosis.
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