1. Isolation, screening and identification of Bacillus spp. As direct-fed microbial candidates for aflatoxin B
Rosario GALARZA-SEEBER ; Juan David LATORRE ; Amanda Drake WOLFENDEN ; Lisa Renee BIELKE ; Anita MENCONI ; Billy Marshall HARGIS ; Guillermo TELLEZ ; Xochitl HERNANDEZ-VELASCO
Asian Pacific Journal of Tropical Biomedicine 2015;5(9):702-706
OBJECTIVE: To evaluate the ability of Bacillus spp. as direct-fed microbials (DFM) to biodegrade aflatoxin B
2. Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens
Rubén MERINO-GUZMÁN ; Xochitl HERNANDEZ-VELASCO ; Juan David LATORRE ; Amanda Desha WOLFENDEN ; Kyle Dean TEAGUE ; Lucas Elzie GRAHAM ; Brittany Danielle MAHAFFEY ; Mikayla Francis Ann BAXTER ; Billy Marshall HARGIS ; Guillermo TELLEZ ; Ramiro DELGADO
Asian Pacific Journal of Tropical Biomedicine 2017;7(2):116-120
Objective To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time points from Days 30 to 89 in white Leghorn chickens. Experiment 2 was conducted to evaluate the effect of 50 or 500 ppb of aflatoxin B