From January 1977 to December 1988, 279 cases of esophageal carcinoma were proved to be stage Ⅰ andⅡ A after operation. Within 5 years of the operation, 103 cases have failed. The failure was closely related to TNM stages. The regional and esophageal bed failure were obviously related to the site of the original lesion. Twelve of these 279 patients recieved postoperative radiotherapy. The incidence of intra-portal recurrence was lower in those who received postoperative radiotherapy than those who did not, although hematogenous metastasis was similar in these two groups. The 5-year survival rate with no evidence of cancer was higher in patients who received postoperative irradiation.

Objective To investigate the mechanism of Deinococcus radiodurans pprI gene in enhancing mice radioresistance to γ-rays by transfecting it in vivo.Methods The male Kunming mice were randomly divided into control group,irradiated group,pCMV-HA transfected group and pCMV-HA-pprI transfected group.The pCMV-HA-pprI plasmid contained pprI gene was injected into the muscle of mice which were exposed to total 6 Gy of γ-ray irradiation.After injection,the in vivo gene electroporation technology was used to transfect the pprI gene into the cells,and Western blot was used to identify the PprI protein,mammalian homolog protein Rad51 corresponding to recA gene downstream of pprI,and protein Rad52.Results In the muscle of the mice of transfected pCMV-HA-pprI group,the protein PprI expressed significantly at 1 d post-irradiation,but there was no expression of pprI gene 7 d post-irradiation and in other groups.In the mice of transfected with pCMV-HA-pprI,the expression of Rad51 protein was significantly increased in the lungs at 1,7 and 14 d post-irradiation,and significantly increased in the liver at 1 and 28 d post-irradiation and increased in the kidneys at 1 and 14 d post-irradition.However,there was no obvious change of Rad52 protein expression in the lungs and livers of mice in all groups.Conlusions The prokaryotic gene pprI could act on the mammalian homologisation analogues rad51 gene downstream of recA gene and then increase the expression level of protein Rad51 which results in the enhancement of radioresistance.

OBJECTIVE: To evaluate the efficacy of Ethosomes encapsulated with 5-FU in treatment of laryngotracheal stenosis in rabbit models. METHOD: The 5-FU ethosome was prepared by the thin film hydration method, and the size distribution and the encapsulation efficiency was investigated. The tracheal mucosa was scraped about 0.5 cm in width with a nylon brush to induce the scar formation in the airway,then animals were divided into three groups: 5-FU ethosome group,5-FU group and saline group. Drugs were injected into scar by paracentesis under endoscope in each group respectively. The severity of stenosis was observed under laryngofiberoscope immediately, 7, 14, 21 days after administration. RESULT: Airway stenosis of 5-FU ethosome group was not significantly different compared with 5-FU group at 7 days after administration, but 5-FU ethosome significantly reduced the airway stenosis at 21 days after administration when compared with 5-FU group and no restenosis was noticed during the observation period. CONCLUSION Ethosomes encapsulated with 5-FU was effective for laryngotracheal stenosis. It is a potentially new method for ameliorating airway stenosis originated from granulation tissue.
Animals ; Constriction, Pathologic ; Fluorouracil ; therapeutic use ; Liposomes ; therapeutic use ; Rabbits ; Tracheal Stenosis ; pathology ; surgery