Objective To understand the cognitive and behavioral characteristics of AIDS between male-male in college students, and to provide evidence for the prevention and control strategies. Methods Using cluster random sampling method, the questionnaire survey including basic situation, the perception of HIV/AIDS of male male actors, sexual behavior and condom use and HIV/AIDS counseling detection, was used to investigate in male students of 8 universities at Beijing fangshan distric. Results A total of 2444 male college students were surveyed, 138 cases with male-male behavior were detected, and the detection rate was 5.65％. The detection rate (18.31％) of the junior college students was statistically higher than that of first-year college students (4.28％) and sophomores (6.52％, P<0.017). The awareness rates of four relevant knowledge about HIV/AIDS for 138 students were 25.36％, 15.22％, 9.42％and 13.77％respectively. The 44.93％male-male in college students had first sexual intercourse were younger than 18 years old. The proportion of students with first time male-male behavior and age <18 years was higher in the first-year college students (58.9％) than that of sophomores and junior college students (30.77％, 26.92％). The incidence rate of bisexuality was 43.48％ in male-male behavior, and 73.91％ was polysexual partners. The correct usage rate of condom was 31.16％. AIDS counseling detection rate was 27.54％. Conclusion The detection rate is higher in students with male-male behavior, and the awareness rate of AIDS-related knowledge is lower. A variety of high risk sexual behaviors are prevalent, so it is necessary to strengthen HIV/AIDS education and HIV/AIDS related knowledge for college students.

There are limited reports available regarding the treatment of amputated noses. This article presented a case of an incompletely amputated nose in a 47-year-old male who was admitted to Dalian University Affiliated Xinhua Hospital in July 2023. He was characterized by an amputated right nasal tip and alar region, with an avulsion area of approximately 4 cm×4 cm. The broad pedicle was connected to the nasal base, approximately 5 mm below the remaining nasal column, and only the nasal column retained a satisfactory arterial blood supply. During the operation, the amputated tissue was implanted in situ, and clindamycin was employed to prevent infection after the operation by intravenous infusion. Bloodletting with flaps, wet application of heparin saline, and massage were employed and all the amputated tissue survived. The appearance of the nose was deemed acceptable. By reviewing relevant literature, the author discussed and summarized the methods of nasal blood supply and treatment for nasal amputated injuries, thereby providing a reference for the management of similar cases.

There are limited reports available regarding the treatment of amputated noses. This article presented a case of an incompletely amputated nose in a 47-year-old male who was admitted to Dalian University Affiliated Xinhua Hospital in July 2023. He was characterized by an amputated right nasal tip and alar region, with an avulsion area of approximately 4 cm×4 cm. The broad pedicle was connected to the nasal base, approximately 5 mm below the remaining nasal column, and only the nasal column retained a satisfactory arterial blood supply. During the operation, the amputated tissue was implanted in situ, and clindamycin was employed to prevent infection after the operation by intravenous infusion. Bloodletting with flaps, wet application of heparin saline, and massage were employed and all the amputated tissue survived. The appearance of the nose was deemed acceptable. By reviewing relevant literature, the author discussed and summarized the methods of nasal blood supply and treatment for nasal amputated injuries, thereby providing a reference for the management of similar cases.

Objective To explore the research status,hot topics and trends in the construction of integrity in public hos-pitals in China,provide references for the research on integrity construction in public hospitals and promote their high-quality de-velopment.Methods The relevant literature on the construction of integrity in public hospitals from 2009 to 2022 was collected from the China National Knowledge Infrastructure(CNKI)database.CiteSpace software was used to analyze the selected litera-ture in terms of publication timeline,author co-citation,institution co-citation,keyword co-occurrence,keyword clustering and keyword burst analysis,and visualize the knowledge graph.Results The research on the construction of integrity in public hos-pitals in China showed an upward trend from 2009 to 2022.The hot topics in the research mainly focused on incorruptible medical practices,integrity risks and integrity culture.The keyword burst analysis revealed that discipline inspection and supervision,in-formation platforms and institutional development were the future research directions.Conclusion The research on the construc-tion of integrity in public hospitals in China has a clear policy orientation,and various subfields are being explored.Internal inde-pendent research is predominant,while collaboration among multiple scholars and institutions needs to be strengthened.The con-struction of integrity in public hospitals in China mainly focuses on three areas.

Objective To investigate the relationship of age-related macular degeneration (AMD) with blood-lipid levels. Methods Individuals 40 years old or older who had undergone a physical-health examination in our hospital between January and December 2017 were enrolled in this study. Information regarding medical history and the results of essential ophthalmological and physical-health examinations were examined to exclude individuals with serious chronic diseases such as malignant tumors, stroke, myocardial infarction, pulmonary heart disease, hypertension, diabetes, and kidney disease. One thousand nine individuals with AMD (all at the early stage) were included in the AMD group, and 3489 individuals without AMD were included in the non-AMD group. Data of all participants, including total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels, were collected and analyzed. Results The average age in the AMD group was higher than that in the non-AMD group, and the male to female ratio was significantly higher in the AMD group (P<0.05). After adjusting for age, gender, and BMI confounders, multiple linear stepwise regression analysis revealed that HDL-C was associated with AMD (β=-0.026, 95% CI: 0.045-0.006, P=0.011); there was no correlation between TC, TG, LDL-C, and AMD (all P>0.05). Conclusion Early stage AMD was related to a decrease in HDL-C, which may be a protective factor against AMD. Further study is warranted to validate this finding.

Objective:To study the level of neutralizing antibodies produced in the population at different time intervals after completion of the inactivated 2019-nCoV vaccine immunization, and to evaluate the protective effect produced by the vaccine in the population.Methods:Fifty volunteers were included in this study, the neutralizing antibodies were detected by ELISA, and the inhibition rate and positive rate were calculated from the absorbance OD value.Results:The positive rates were 76% (38/50), 68% (34/50), 30% (15/50) and 100% (50/50) in the groups of 6, 8, 10 months’ intervals and intensive immunization, respectively; while the mean suppression rates were 58.80%, 49.86%, 34.60 and 95.55%, respectively. The result of the time main effects test showed that there was a statistically significant difference in the mean inhibition rate between the different time periods (F = 326.00, P<0.001). The paired t-test showed a gradual decrease in the mean inhibition rate in the 6th, 8th and 10th month, with a significant increase ( P<0.001) after 7-day in intensive immunization. Conclusions:This study showed after two doses of the 2019-nCoV vaccine, the level of neutralizing antibodies gradually decreased and weakened over time, but there was immune memory in the body, and when the body intensively stimulated by vaccine again, the level of neutralizing antibodies would rise significantly.

Objective:To investigate the relationship between carotid atherosclerosis(CAS)and subclinical left ventricular(LV)dysfunction in type 2 diabetes mellitus patients with preserved LV ejection fraction(LVEF).Methods:A total of 120 patients with type 2 diabetes mellitus who had LVEF≥50% were selected in the Department of Endocrinology, the First Affiliated Hospital of Air Force Medical University from June 2021 to October 2021. The global longitudinal strain(GLS)was obtained by two-dimensional speckle tracking echocardiography(STE)to assess subclinical LV systolic function. The mitral ratio of peak early to late diastolic filling velocity(E/A), and mitral velocity to early diastolic velocity of the mitral annulus(E/E′)ratio were obtained by pulsed tissue Doppler echocardiography to assess LV diastolic function. Acrroding to bilateral carotid ultrasound examination, the subjects were divided into normal carotid arteries group( n=46) and CAS group( n=74). Demographics and biochemical parameters were compared between two groups. Binary logistic regression and Pearson correlation analysis were used to evaluate the relationship between CAS and subclinical LV dysfunction. Results:The CAS group had a higher proportion of men, older age, and a longer duration of diabetes than the normal carotid arteries group(all P<0.05). There was no difference in LVEF and GLS between the two groups [normal carotid arteries group vs CAS group, LVEF: (60.72±4.73)% vs(60.07±4.28)%; GLS: (18.24±3.72)% vs(17.81±3.47)%, respectively; both P>0.05]. However, compared with normal carotid arteries group, E/A ratio was decreased and E/E′ ratio was significantly increased in CAS group(both P<0.01). Pearson correlation analysis showed that GLS was not correlated with carotid plaque thickness and carotid intima-media thickness(CIMT; both P>0.05). By contrast, E/E′ ratio was positively correlated with carotid plaque thickness and CIMT(both P<0.05). Binary logistic regression analysis showed that GLS and E/E′ ratio were not associated with CAS( both P>0.05). However, decreased E/A ratio was significantly associated with the existence of CAS( OR=0.09, 95% CI 0.01-0.67, P=0.018). Conclusions:In type 2 diabetes mellitus patients without overt heart failure and with preserved LVEF, the occurrence of CAS is not associated with subclinical LV systolic impairment assessed by GLS, but is significantly associated with LV diastolic dysfunction, and is independent of traditional cardiovascular risk factors.

Objective To investigate the effect of glycine N-methyl transferase (GNMT)on intrauterine adhesion (IUA)fibrosis and its related mechanism.Methods In vivo experiment:A total of 36 healthy female SD rats (SPF grade,6～8 weeks old and weighing from 180～220 g)were subjected in this study.IUA model of SD rats and IUA model of GNMT overexpressed rats were established.RT-qPCR and immunofluorescence assay were applied to detect GNMT expression level in normal uterus and model group.RT-qPCR and Western blotting were used to detect the mRNA and protein levels of fibrosis-related molecules and the activation of TGF-β1/Smad3 signaling pathway in each group.The number of endometrial glands in each group was observed by HE staining.Masson staining was used to analyze the severity of endometrial fibrosis in each group.In vitro experiment:transformed human endometrial stromal cells (THESCs)fibrotic phenotype model was constructed using TGF-β1,and THESCs stably transfected with GNMT overexpression lentvirus were treated with TGF-β1.RT-qPCR and Western blotting were used to detect the mRNA and protein expression of fibrosis-related molecules.The expression of TGF-β1/Smad3 signaling pathway was detected by Western blotting.TGF-β1/Smad3 signaling pathway was activated by TGF-β1/Smad signaling pathway activator (SRI-011381),and the expression of TGF-β1/Smad3 signaling pathway and key molecular proteins of fibrosis phenotype was measured with Western blotting.Results In vivo experiment,the mRNA and protein expression levels of GNMT were significantly decreased in the IUA rats than the control rats (P<0.05).Overexpression of GNMT decreased the mRNA and protein levels of fibrosis related molecules,Collagen Ⅰ,Collagen Ⅲ and FN in the IUA rats (P<0.05),and decreased the phosphorylation levels of TGF-β1 and its downstream Smad3 protein (P<0.05).HE and Masson staining showed that overexpression of GNMT could increase the number of endometrial glands and reduce the severity of fibrosis in the IUA rats (P<0.05).In vitro experiments:overexpression of GNMT decreased the mRNA and protein levels of Collagen Ⅰ,Collagen Ⅲ and FN associated with fibrotic phenotype of THESCs (P<0.05),and reduced the phosphorylation level of Smad3 protein,downstream of TGF-β1 (P<0.05).After activation of TGF-β1/Smad3 signaling pathway,the protein levels of TGF-β1/Smad3 signaling pathway and downstream fibrosis phenotype molecules,Collagen Ⅲ and FN,were significantly decreased in the LV-GNMT+SRI-011381 group.Conclusion Overexpression of GNMT can inhibit endometrial fibrosis by regulating TGF-β1/Smad3 signaling pathway,thus achieving therapeutic effect on IUA.

Objective: To investigate the status of depression and anxiety symptoms in mothers with autistic child, and explore the association between coping styles and emotional problems.To provide theoretical evidence of mental health intervention for mothers with autistic children. Methods: A cross-section study was conducted in three autism rehabilitation centres of Changsha city.A total of 135 mothers of autistic child were recruited from March to November 2018.The self-designed questionnaires, patient health questionnaire-9 (PHQ-9), generalized anxiety disorder scale-7 (GAD-7), simple coping style questionnaire(SCSQ) and autism behaviour checklist(ABC) were used in the study.Spearman analysis was used to explore the correlation between emotional problems and copying styles.The ordinal logistic regression was conducted to explore the impact of the coping styles for emotional problems. Results: The scores of anxiety and depression symptoms were 9.0 (5.0, 13.0) and 7.0 (3.0, 11.0), respectively.The occurrence rate of anxiety and depression symptoms was 44.5% and 28.1%, respectively.The scores of positive and negative coping were (1.75±0.90) and (1.05±1.03), respectively.There were 72 (53.3%) and 63 (46.7%) of participants adopted positive and negative coping styles, respectively.There was a positive correlation between negative coping and anxiety symptoms (r=0.182, P<0.05). There was a negative correlation between positive coping and depression symptoms (r=-0.184, P<0.05). The ordinal logistic regression showed positive coping was a protective factor for anxiety symptoms (OR=0.444, 95%CI: 0.205-0.965). Conclusion The anxiety and depression symptoms are common problems in mothers of children with ASD.It is necessary to take suitable psychological interventions to encourage them to adopt positive copying styles.