Vascular remodeling is an important pathological link of maintaining and developing high blood pressure. TCM has the advantage of comprehensive regulation effects with multiple channels, multiple links and multiple targets. Applying TCM treatment in vascular remodeling is a good point cut in the prevention and treatment of hypertention. This article reviewed the research status of TCM treatment of vascular remodeling in hypertention, with a purpose to provide ideas for clinical application and further research.

Myocardial fibrosis is the pathological basis of hypertensive heart disease, which is the key to control cardiovascular complications. Traditional Chinese medicine (TCM) has the advantage of targeting many components of a system and providing more integrated regulation than modern medicine. While, modern medicine tends to target a single pathological link. This study was aimed to investigate effects and mechanisms in the treatment of myocardial fibrosis in hypertension with TCM from a different perspective, which provided references for clinical treatment.

Moon tidal flow Naja-Law is a calculating method for choosing acupoint according to time, is a model of chronotherapeutics of TCM. But because the Najia method of calculation is complex, the clinical application is limited. The author based on years of teaching experience, summed up the calculation procedure of three steps: the first step, calculating the heavenly stems, determining the relationship between duty in accordance with the heavenly stems and the meridians; the second step, according to the rules of acupoints, deducing five transporting points; the third step, calculating the acupoint hour.

The connotation and extension of the acupuncture changes along with the development of science and technology. Working out the principle on the connotation and extension of acupuncture at present will contribute to better inherit and development of acupuncture. In this paper, through literature analysis, the study on the process of formation and development of acupuncture, we believe connotation of acupuncture and moxibustion mainly include the basic theory of TCM, meridians theory, diagnosis, techniques, clinics, and literatures of acupuncture and moxibustion;and its extension include basic integrated medicine of acupuncture and moxibustion, physiology, pathology, and theories, techniques and clinics derived from modern science.

Objective To investigate the predictive value of platelet parameters on distant metastasis of different clinical stages of non‐small cell lung cancer (NSCLC) .Methods A retrospective analysis was performed on the inpatients with lung cancer in our hospital from April 2013 to January 2014 .The parameters of platelet count (PLT ) ,mean platelet volume (MPV) ,platelet distribu‐tion width (PDW) ,platelet ‐large cell ratio(P‐LCR) ,platelet hematocrit (PCT ) ,etc .were detected respectively .Results The re‐sults showed that PLT ,MPV ,P‐LCR and PCT in the lung cancer group were higher than those in the benign lung tumor group and the healthy control group ,the differences were statistically significant (P< 0 .01) ,while PDW had no statistically significant differ‐ence among these groups(P> 0 .05) .With the TNM stage increase ,the increased platelet proportion in the stage Ⅲ and Ⅳ was sig‐nificantly higher than that in the stage Ⅰ and Ⅱ (32 .35% ,42 .85% vs .19 .35% ,22 .85% ,P< 0 .01) .The platelet five parameters in the stage Ⅳ had statistical difference compared with those in the stage Ⅰ and Ⅱ (P< 0 .05) ;but PDW and P‐LCR in the stage Ⅲhad no statistical difference compared with those in the stage Ⅰ and Ⅱ (P> 0 .05) ;PLT ,MPV and PCT in the stage Ⅳ had statisti‐cal difference compared with those in the stage Ⅲ (P< 0 .05) .Conclusion The patients with lung cancer often stimulate the bone marrow megakaryocytes to produce platelets by a particular mechanism .As the staging increase ,the proportion of increased platelet is more obvious .Continuously and dynamically observing platelet parameters is conducive to judge the development and prognosis of the disease .

Objective To prepare targeted microbubbles which load anti-VEGFR-3 and observe the targeting effect in vitro.Methods Targeted microbubbles loaded VEGFR-3 antibody were prepared and the targeting effect in vitro were tested by direct and indirect methods.Direct method took off-line analysis by image analysis software IPP and statistical analysed with SPSS software package.Indirect method took direct observation the target binding effect after incubation and centrifugal sedimentation.Results Image software IPP results showed that the quantity of microbubbles in targeted-group was obviously more than ordinary group.The average microbubbles per field in targeted-group was (151 ±20).The average microbubbles per field in ordinary group was ( 12 ±4 ) microbubbles, the numbers of combined microbubbles between targeted-group and ordinary-group had significant differences ( t=19.19,P<0.01).The indirect method results showed that, compared with ordinary group, the microbubbles in targeted-group could combined with cell gradually.Conclusion Targeted microbubbles load anti-VEGFR-3 have a better contrast effect than common microbubbles.

Objective To discuss whether mild hyperuricemia can lead to kidney damage and the protection of decreased uric acid,through observing that hyperuricemia did damage to glomerulus endothelial function and cell proliferation of vascular smooth muscle in rats. Methods Fifty-four male SD rats were divided into four groups,the control group,model group (Oxonate),allopurinol group and Oxonate+allopurinol group.Rats were administered on a low sodium diet and their systolic blood pressure (SBP) were measured each 10 days.ELISA was used to detect rat plasma markers of endothelial function damage [nitric oxide (NO),type-1 plasminogen activator inhibitor (PAI-1),endothelin 1 (ET-1)] and cell proliferation of vascular smooth muscle[plateletderived growth factor (PDGF),cycloxygenase 2 (COX2),monocyte chemotactic protein-1 (MCP-1)],and the markers of inflammatory reaction[interleukin-18 (IL-18),tumor necrosis factor α(TNF-α)].PDGF and nitric oxide synthase (NOS) levels of rats were detected by immunohistochemical method.Renal tissue pathology of rats was observed. Results Compared to the control group,the plasmic concentration of COX2,ET-1,IL-18,PAI-1,PDGF,TNF-o,MCP-1 increased,and NO decreased significantly in rats of model group (all P＜0.05),expression of NOS significantly reduced and PDGF increased (all P＜0.05).Under light microscope,vascular wall thickening,intimal proliferation and lumen slight stricture without uric acid crystals in renal tissue were found in model group,which were obviously improved by using allopurinol. Conclusion Mild hyperuricemia can do damage to endothelial function of glomerulus and lead to vascular cell proliferation,which can be improved through decreasing uric acid.

OBJECTIVETo explore the technique of temperature control on the abdomen in penetrating moxibustion through observing moxibustion time on the abdomen, abdominal skin temperature and effect after moxibustion so as to provide the safe, effective and easily applicable method for penetrating moxibustion.

METHODSThirty-two patients were selected in an observation group, 32 healthy persons in a control group. In the observation group, the penetrating moxibustion was applied to the corresponding acupoint locations according to different symptoms. In the control group, moxibustion was used on the abdomen around the umbilicus. The skin temperature was recorded once every minute. The skin temperature of known heat sensation, the time of known heat sensation, the known reduced temperature, the time of temperature reducing, the skin temperature difference, the duration of penetrating moxibustion and the reaction of moxibustion from participants were recorded.

RESULTSThe differences in the skin temperature of known heat sensation, the time of known heat sensation and the duration of penetrating moxibustion were significant statistically in comparison between the observation group and the control group (all P<0.01). The differences in the known reduced temperature, the time of temperature reducing and the skin tem- perature difference were not significant (all P>0.05). The differences were significant statistically in skin rashes and moxibustion reaction (gastrointestinal peristalsis, chills, ant climbing feeling and hunger, etc.) between the two groups (P<0.01). The differences were not significant statistically in flushing, sweating and blisters (all P>0.05).

CONCLUSION(1) The level of temperature sensitivity in the observation group is lower than that in the control group. During penetrating moxibustion, the sensations such as gastrointestinal peristalsis, chills, ant climbing feeling and hunger appear easily, suggesting the positive self-adjustment in the body. (2) During penetrating moxibustion, the warm feeling is penetrated not just from the epidermis to the abdominal cavity and lumbar region, but also up to thehead and down to the knee. (3) The flushing, sweating and skin rashes are the important indices for the effectiveness of penetrating moxibustion. (4) The temperature control is the core technique of penetrating moxibustion. The penetrating moxibustion in 28 min to 32 min and the temperature controlled in 43 degrees C to 45 degrees C can solve the moxibustion smoky impact to the environment, but also relieve pains of the patients.

Abdomen ; physiology ; Acupuncture Points ; Adult ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; Skin Temperature ; Thermosensing ; Young Adult

Objectives To observe the effect ofGuizhi-Fuling capsule on the expression of AngⅡand eNOS.Methods 72 male Sprague-Dawley(SD) rats, aged three months, were randomly divided into three groups: a sham-operated group(n=24),a model group (n=24) and a treatment group (n=24). Renal tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO). Rats in the treatment group were treated withGuizhi-Fulingcapsule (125mg/kg),and the same volume of normal saline were given to the rats in the sham -operated group and the model group at the same time. Rats were put to death at day 7, 14, 21 Immunohisto-chemistry staining was performed to investigate both the expression of AngⅡ, eNOS in TIF. Results 1 Renal interstitium injury was seen in the model group and the treatment group rats at day 7. The degree of renal interstitium fibrosis in rats from the treatment group was lighter than that of the model group at day 7, 14, 21 (P< 0.01).2 At day 7,14 and 21, the expression of AngⅡin rats from the model group and the treatment group were higher than that of the sham-operated group (P<0.01), but those of the treatment group was lower than those of the model group (P< 0.01). The expression of AngⅡ in rats from the model and the treatment groups were increasing,the highest in the 21th day (P<0.01).3 At day 7, 14 and 21, the expression of eNOS in rats from the model group and the treatment group were lower than that of the sham-operated group  (P<0.01).ConclusionsGuizhi-Fuling capsule can alleviate the lesion of renal tissue in obstructive kidney and delay the development of TIF.