Objective: To evaluate the effect of standard management for hypertension in community population of Suzhou city. Methods: We conducted a prospective study of standard management for hypertension in community population of Suzhou city. The project included 3 groups: Standard group, the patients received standardized management,n=81 711, Control group, the patients received general management, n=38 944, and No-management group,n=1 644 249. The incidence rate of stroke and coronary artery disease (CAD) were compared among different groups according to 2010 chronic disease report of Suzhou city. Results: Compared with initial condition, the final average blood pressure drop in Standard group was (13.78/7.06) mmHg, which was better than that in Control group, X2=1852.91,P<0.01. The incidence rate of stroke in Standard group, Control group and No-management group were 1.72%, 2.41% and 3.59%, the incidence rate of CAD were 0.43%, 0.66% and 1.29% respectively. Conclusion: Standard management for hypertension was better than general management for preventing and controlling the incidence of stroke and CAD in community population.

OBJECTIVE To investigate the variation of in vitro activity,the ?-lactamases,type diversity and the homology of multiple resistances in Acinetobacter isolated.METHODS The multiple resistant Acinetobacter were selected to detect susceptibility test by K-B antimicrobial agents.The resistant rates were analyzed by WHONET 5.4,the isolates ?-lactamases phenotype was detected by three-dimensional test,genomic types were measured by PFGE.The ?-lactamases genotype was determined by PCR assay with specific-primer,and DNA sequencing was also used to analyze resistance-related gene.RESULTS Twenty-eight of 45 strains were OXA producing strains(68.3%),10 strains were IMP producing strains(24.4%),13 strains were TEM producing strains(31.7%),18 strains were CTX-M producing strains(43.9%),6 strains were PER producing strains(14.6%),and 7 strains were AmpC producing strains(17.1%).None produced SHV ?-lactamases.Twenty-four strains were produced 2 or more than 2 kinds of ?-lactamases.CONCLUSIONS The multiple resistance of Acinetobacter can produce kinds of ?-lactamases,but producing ?-lactamases are not the only one mechanism.

Obiective To develop a new medical image processing platform based on ITK and OpenGL. Methods This platform was designed by VC ++ 6.0 and MFC class library. ITK class library was wsed to fulfill reading, writing,segmentation of images and iso-surface extraction. OpenGL was wsed to realize 3D surface rendering. Results The new 3D-medical imaging reconstruction system was designed. Conclusion This system not only can provide graphic interface which can not be realized by using ITK class library, but also enhance the ability of three-dimension visualization by using OpenGL.

Objective　To select suitable media for increasing isolation rate of haemophilus.Methods　Haemophilus was inoculated on seven types of media.The average growth index(GI) of Haemophilus was calculated and Haemophilus was isolated from 325 specimens of the respiratory tract and its isolation rate was compared with that media of brchoc and brchoc-V agare.Results　The GI of media with 2% fresh yeast infusion and 50% of meat infusion was higher than that of those with no yeast and meat infusion.The GI of Haemophilus on BRchoc was higher than that of the BSchoc(P<0.001).The isolation rate of Haemophilus from 325 respiratory tract specimens on BRchoc-V was 77.2%,which was significantly higher than that on the medium BRchoc (32.0%).Conclusions　BRchoc-V is a better medium for isolation of Haemophilus.This medium is effective to improve the isolation rate of Haemophilus.

Objectives To evaluate mini/VITAL and BacT/Alert, two fully automated blood culture systems. Methods Standard mini/VITAL AER and ANA bottles (bioM′erieux, Marcy 1′Etoile, France) and BacT/Alert FAN aerobic and FAN anerobic bottles (organon Netherlands) were used. The bottles were analysed over a period of 7 days. A total of 600 samples of blood, 108 samples of body fluid, and 100 strains of imitation were detected by mini/VITAL and BacT/Alert parallelly. The bottles of flagged positive were smeared and Gram stained before being examined under a microscope. Subcultures were performed in parallel on fresh blood agar and chocolate agar incubated in microaerophilic Conditions with 5% CO 2. All the bottles flagged negative were examined in the same conditions as the positive bottles. Results mini/VITAL: The positive rate for aerobic and anaerobic blood cultures was 10% and 5% respectively, for aerobic body fluid culture was 26 9%. No contaminants were detected. 0.5% false positive and 0.8% false negative were detected. 90% of positive sample were detected in 48 h. The shortest time of report of positive bottles was 1.5 h for blood culture. BacT/Alert: The rate of positive of aerobic and anaerobic blood culture was 9.8% and 5% respectively. The positive rate of body fluid culturs was 26.9%. There were used 3 bottles contaminated. The false positive and false negative rate were 0.5% and 0 98% respective. 95.5% of positive bottles were detected in 48 h. The shortest time of flagged positive was 2.5 h. Conclusion mini/VITAL and BacT/Alert were accurate automated blood culture systems for the detection of bacteremia. There was no significant difference on the speed of detection between BacT/Alert FAN bottles and mini/VITAL standard bottles.

Objective To understand the distribution of anaerobes in gingivitis infection, and anaerobes sensitivity to ? lastams Methods 44 specimens were taken from the gingival crevicular fluid of gingivitis. The anaerobic bacteria were cultured and identified Minimal inhibitory concentrations (MIC) were measured by using agar dilution method Results Among all samples, the isolation of anaerobes was 100%, the isolation of P.melaninogenica was 86.4%, P.intermedia was 56.8%, B.forsythus was 52.3%, P.gingivalis was 65.9%, F.mucleatum was 63.6%, P.micros was 52.3%, Actinomyces ssp was 38.6% and Capnocytophaga ssp was 9.1% respectively. The anaerobes are sensitive to ? lastams. Conclusion In gingival crevicular fluid, the infection rates of P.melaninogenica, P.intermedia, B.forsythus, P.gingivalis, F.mucleatum, P.micros, and Actinomyces ssp were significantly higher than those of the other anaerobes. ? lastams can be used for the treatment of anaerobes infected gingivitis.

OBJECTIVE The in vitro activity of home-made rifamycin was investigated.METHODS Minimal inhibitory concentrations(MICs) were determined by agar dilution method using multipoint inoculator.RESULTS The results indicated that MIC50 and MIC90 of rifamycin against MRSA and MSSA were similar to vancomycin. The values were 1 and 2 mg／L, respectively. Rifamycin against MRSE was 4 times stronger than vancomycin.The value of MIC50 was 0.5 mg／L.The MIC50 of rifamycin against S.pneumoniae,Branhamella catarrhalis,and Listeria monocytogenes were 8,128 and 4 times lower than vancomycin, respectively.CONCLUSIONS There are good antibacterial activity of home-made rifamycin against meticillin resistant Staphyloccocus and the most species of clinical isolates,so can be used the infective diseases by MRSA and MRSE.

494 ng/ml. The test showed a sensitivity of 100%, a negative predictive vale of 100%. Conclusion VIDAS D dimer is well sited as diagnostic tool for the exclusion pulmonary embolism.

Objective To understand the carriage of NDM-1 and other carbapenemases in carbapenem-resistant Acinetobacterbaumannii(CRAB)in Jiangxi area,and provide laboratory basis for the prevention and control of healthcare-associated infection (HAI). Methods Sixty-four strains of CRAB isolated from clinical specimens from 3 tertiary first-class hospitals in Jiangxi area from January 2015 to June 2016 were collected,susceptibility to com-monly used antimicrobial agents were detected with Kirby-Bauer method. Carbapenemases and metalloenzyme in CRAB were screened with modified Hodge test and EDTA-disk synergy test respectively,carbapenems gene was de-tected by polymerase chain reaction (PCR),NDM-1-producing Acinetobacterbaumannii (A. baumannii)were per-formed conjugation test.Results The resistance rates of CRAB to ampicillin/sulbactam,ciprofloxacin,gentamicin, and levofloxacin were up to 95.31% ,98.44% ,90.63% ,and 54.69% respectively. The positive rates of modified Hodge test and EDTA-disk synergy test were 76.56% and 96.88% respectively. PCR amplification result showed that 87.50% (n= 56)of CRAB carried OXA-23 and VIM-1 genes,18.75% (n= 12)carried SIM,3.13% (n= 2)car-ried OXA-24,and 26.56% (n= 17)carried NDM-1 . CRAB carrying NDM-1 gene were all from The First Affilia-ted Hospital of Nanchang University,64.70% (11/17)of which were pandrug-resistant strains. Conjugation test re-sult showed that NDM-1-producing strains could transfer NDM-1 gene to recipient strain Escherichiacoli J53,then acquired resistance to imipenem. Conclusion Antimicrobial resistance rates of clinically isolated CRAB in this area are high,OXA-23 and VIM-1 genes are the main carbapenemase genes,NDM-1 gene positive CRAB is detected, and there may be a clonal spread of NDM-1 gene in hospital,effective measures should be taken as soon as possible to prevent and control the spread of NDM-1 positive CRAB.

OBJECTIVE To determine the mutant prevention concentration(MPC) of 3 cephalosporins against clinical isolates of Staphylococcus aureus and Streptococcus pneumoniae.METHODS Agar dilution was used to determine the minimum inhibitory concentration(MICs) and MPCs of cefaclor,cefprozil and cefuroxime against S.aureus and the MICs to Str.pneumoniae.The MPCs against Str.pneumoniae were determined by mixing-bacterium.RESULTS The MPCs and the ratio of MPC90/MIC90 of cefaclor,cefprozil and cefuroxime against S.aureus were 32,16;16,16;2,8;and the MPCs and the ratio of MPC90/MIC90 against Str.pneumoniae were 8,16;4,32;2,8,respectively.CONCLUSIONS The ability of cefuroxime for restricting the selection of resistant mutant of Str.aureus and S.pneumoniae is stronger than cefaclor and cefprozil.