Objective To compare the protective effect of trehalose with that of sucrose in lyophilization of red blood cells. Methods Concentrated red blood cells and 30%PVP containing different concentrations (5% ,10% and 15%) of trehalose (or sucrose) were mixed at a ratio of 1 : 3, pre-frozen at - 80℃ for 1h.then lyophilized in freeze-dryer. After lyophilization, the samples were reconstituted and washed in 37℃ isotonic buffer. The experiment was divided into 3 groups: 30%PVP group,trehalose group (containing 5%, 10% and 15% trehalose) and sucrose group (containing 5% , 10% or 15% sucrose). Results After lyophilization and rehydration, the recovery of red blood cells in trehalose groups were (18.86 ?4.63) %, (21.73 ? 7.32) % and (18.01?4.53) % respectively and significantly lower than that of PVP group[(45.97+11.77)%](P

Objective:To investigate the knowledge, attitudes and practice of child sexual abuse (CSA) prevention education in parents of elementary school pupils.Method:Two hundred and seventy one parents were surveyed by anonymous self-administered questionnaire in five classes of grade 1 to grade 5 of a elementary school. The questions about awareness of CSA and communications on how to prevent CSA with their children were asked.Result:Among this sample, 93.6% of parents approved of school CSA prevention education. Overall, about half parents lack of knowledge of CSA prevention, the communications with their children were also not enough. Only 1.8% of parents had provided books or audiovisuals about CSA prevention for their children. The scores of knowledge, attitudes and practice of CSA prevention education of parents whose parents addressed this in their childhood were significantly higher than those of parents who had no this experience.Conclusions:The research of CSA prevention education in elementary school should be paid more attention, to produce related books and audiovisuals as soon as possible, to increase parents' awareness of CSA prevention.

Objectives To maximize the survival of lyophilized red blood cells after reconstitution.Methods The colloidal and crystalloid osmotic pressures of seven reconstitution solutions,including 10% polyvinylpyrrolidone(PVP),6% hydroxyethyl starch (HES),5% carboxymethylcellulose sodium (CMS),0.9%NaCl,0.75mol/L Glucose,isotonic buffer ,and hypertonic buffer (5?buffer),were measured. After washing and equilibrating,red blood cells were mixed with protective solution according to the ratio of 1∶3,then pre frozen at -80℃ for 1h,at last,samples were lyophilized in freeze dryer.After lyophilization,the samples were reconstituted in different reconstitution solutions and at different temperatures,the red blood cell recovery was measured.Results After lyophilization and reconstitution,the recovery of red blood cells in 6%HES,10%PVP,and 5%CMS were (93.65?6.18)%,(88.80?9.49)% and (91.34?8.13)%,respectively,and the recovery of hemoglobin were (93.48?4.67)%,(89.02?4.67)%,and (88.79?)5.35% respectively,significantly higher than those of the other groups[(15.56?12.02)%～(27.77?6.48)%,(17.78?10.80)%～(41.50?6.43)%] (P

OBJECTIVE To investigate the occupational hazard situation among medical staff and conduct effective measure and enhance the medical care.METHODS A questionnaire survey on occupational hazard had been completed among the hospitals in Fengtai District in Beijing in three years.RESULTS The rate of sharp damage of medical staff was 72.13%,from them the nurses were with highest rate(81.49%).The higher rate of sharp damage was in the operating theater(88.32%)and the surgical department(82.49%).CONCLUSIONS To assure the medical safety,we should pay more attention to the occupational hazard management.

Objective To observe the effects of calcitonin on osteoclast activity and expressions of osteoprotegrin (OPG) and receptor activator of NF-?B ligand (RANKL) in vitro. Methods Osteoclasts and their precursors were mechanically isolated from long bones of SD rats and incubated on bone sheet in MEM with different concentration of calcitonin. Number, morphology of the osteoclasts were detected with tartrate-resistance acid phosphatase (TRAP) staining. Image-Pro Plus counted the resorption pits and areas on slices, stained by toludine blue. RT-PCR was used to examine the expressions of OPG and RANKL of osteoclast. Results Number of osteoclasts were decreased with increasing CT concentrations. After incubation for 5 days, resorption pits were decreased in a concentration-dependent manner (P

目的探讨心理支持对焦虑症患者康复的影响。方法60例焦虑症患者随机分为研究组和治疗组各30例,研究组接受药物和心理支持治疗,对照组采用单纯药物治疗,评定两组患者的疗效。结果治疗后,两组患者的焦虑自评量表(SAS)、汉密顿焦虑量表(HAS)评分均明显降低,但研究组的降低幅度明显大于对照组(P<0.01～0.001)。结论心理支持对焦虑症患者的康复有明显的促进作用,近期疗效明显。

A 53-year-old man presented with prunosus nodules and plaques on the trunk and extremities for half a month and with periorbital swelling for 4 days.Hematological examination in a local hospital showed thrombocytopenia,and pulsed corticosteroid therapy did not work.On physical examination,there were splenomegaly and multiple enlarged superficial lymphnodes.Serum calcium and 1-lactate dehydrogenase (LDH)were increased to 3.12 mmol/L and 853 U/L,respectively.Serum immunofixation electrophoresis evidenced the presence of a monoclonal immunoglobulin IgM (κ chain).Positron emission tomography (PET-CT) showed abnormal uptake in multiple lymph nodes,back wall of the pharynx,and spleen.The biopsy of a nodule in the neck revealed a diffuse infiltration of numerous atypical lymphoid cells in the subcutaneous fat tissue,which were medium-sized with round nuclei,obvious nucleoli and karyokinesis, Immunophenotyping of the abnormal lymphocytes indicated positive reactions for L26,CD79a,Bcl-2,cyclin D1,multiple myeloma oncogene 1 (partly),Ki-67 (＞80％),but negative for CD5,CD21,CD23,CD38,CD3,CD10,Bcl-6,CD45RO,terminal deoxynucleotidyl transferase (TdT),myeloperoxidase (MPO),CD30,anaplastic lymphoma kinase (ALK),CD117,or CD34.Fluorescence in situ hybridization (FISH) revealed the presence of a fusion gene (t(11:14) CCND1/IGH) in the abnormal lymphocytes.Based on the above findings,the diagnosis was made as blastoid mantle cell lymphoma with skin and periorbital involvement complicated by hypercalcemia.After treatment with rituximab injection,cyclophosphamide,vincristine,doxorubicin,dexamethasone,and intermittent treatment with intravenous high dose of methotrexate and cytarabine (R-Hyper-CVAD),serum calcium returned to a normal level three days later,and the patient made a quick and excellent recovery on the 6th day,with the regression of skin lesions and poriorbital swelling.Unfortunately,the patient eventually died of severe pulmonary infection one month later.

The concept is cell of the theory. Starting from the concept, is the first and important step in building the modern basic theory of traditional Chinese medicine. Based on the early thought of Changes from phenomenon description to entity elucidation, we firstly introduce conceptual change in this paper and point out its basic meaning and role. To accurately apply the new concept, we have analyzed and demonstrated the concept and its expression object, the concept words' expression forms, as well as the concept explanatory power in details, and pointed out the problems because of ignoring these recognition in researching the concept of chinese medicine and its solutions. We have summarized the laws of the international scientific community realizing the conceptual change focus on the object, explore the unknown, propose new knowledge. and proposed the definition of conceptual change of the basic theory of traditional Chinese medicine. According to preliminary findings we have generalized the goal and standard to achieve the conceptual change of the basic theory of traditional Chinese medicine and gave examples of proof. As such we have draw an conclusion: the concepts of the basic theory of traditional Chinese medicine form the scientific concepts of the modern basic theory of traditional Chinese medicine through conceptual change, and thus guiding the theory, teaching and thinking reform of the Chinese medicine.

Famous TCM gynecological experts in the contemporary era represent the highest level of TCM gynecology during modern age.This article studies the academic thoughts of eight famous TCM gynecologic experts according to academic schools.The purpose is to seek the commonness of these academic thoughts and research their characteristics.It is extremely significant in inheriting and developing these doctors′ academic thoughts without sectarianism,which will help to guide the clinical works.