Papers on big data studies in domestic libraries were analyzed with g index and cluster analysis method with CNKI as the data source,which showed the following hotspots in studies on big data,namely innovative service, subject service and literature resource development , big data storage , big data process , big data mining and analy-sis, big data processing technology and tools.The problems waiting to be solved were pointed out.

Objective To analyze the relevant factors of vaginal delivery postpartum hemorrhage,and discussion how to prevent postpartum hemorrhage.Methods 2 417 maternal women who hospitalized vaginal delivery were selected.Preeclampsia,macrosomia,placental abruption,obesity,premature rupture of membranes,high blood pressure,gestational age,maternal age,maternal time,the number of abortion,scar uterus vaginal delivery,oxytocin induced labor,misoprostol for cervical mature,forceps midwifery,and the correlation of postpartum hemorrhage were analyzed.Results The incidence rate of postpartum hemorrhage was 15.22%.Pre -eciampsia,macrosomia,placental abruption had significant association with postpartum hemorrhage(χ2 =26.75,0.16,22.26,all P <0.01).Obesity, premature rupture of membranes were associated with postpartum hemorrhage(χ2 =6.53,4.98,all P <0.05).High blood pressure,gestational age,maternal age,maternal time,the number of abortion,scar uterus vaginal delivery had no statistical correlation with postpartum hemorrhage (P >0.05 ).Oxytocin induced labor,misoprostol for cervical mature,forceps midwifery were significantly associated with postpartum hemorrhage(χ2 =45.66,21.77,88.06,all P <0.01 ).Conclusion Prenatal maternal and neonatal weight control,prevention of preeclampsia,placental abruption occurred to prevent postpartum hemorrhage;Intrapartum avoid no indications oxytocin,misoprostol for cervical mature,forceps midwifery and reduce postpartum hemorrhage;Postpartum accurately estimated blood loss, active treatment,avoid serious complications.

Objective To investigate relationship between the level of serum high sensitive C-reactive protein (hs-CRP) and severity extent,risk factors of acute cerebral infarction. Methods The serum hs-CRP was measured in 186 patients with atherothrobotic cerebral infarction(ACI), in 155 patients with lacunar infarction (LI) and in 329 normal controls by automatic analyzer, levels of blood glucose and blood lipid were detected at the same time. The relationship between hs-CRP and the degree of nerve damage in patients with acute cerebral infarction observed and analyzed. Results The levels of hs-CRP in patients with ACI and LI were higher significantly than that in the normal controls(all P

Objective: To explore diagnostic value of dynamic electrocardiogram (DCG) on coronary artery disease. Methods: A total of 100 inpatients with “angina pectoris” history from our hospital were enrolled and received 24h DCG measurement. The DCG examination results were compared with those of coronary angiography (CAG) and analyzed then. Results: Among the 100 patients, there were 85 patients with positive DCG, including 70 cases with positive CAG and 15 cases with negative CAG; and 15 patients with normal (negative) DCG, including 10 cases with negative CAG and five cases with positive CAG. With CAG as golden standard, sensitivity, specificity, positive predictive value (accuracy) and negative predictive value of DCG in diagnosis of coronary heart disease were 93.3%, 40.0%, 82.4% and 66.7% respectively. Partial patients had “angina pectoris”, no stenosis in CAG but there’s slow blood flow. Conclusion: Dynamic electrocardiography is one of important measuring methods diagnosing coronary heart disease. It is simple, practical, safe, economic and appropriate for wide application in clinic, but its specificity is just 40.0%, so coronary heart disease cannot be excluded in negative patients.

Objective To study the visual attention of mild cognitive impairment patients (MCI)by eyes movement training. Meathods 48 mild cognitive impairment patients were divided into eye movement training group,finger training group and untreated control group by single-blind randomized block method. Then they were trained respectively three months. Event-related potentials (ERPs) were used to measure the face recognition of them before and after training. The amplitude and latency of P3b elicited by target stimuli and P3a elicited by new stimuli of the three said groups were compared and contrasted. Results After training,the amplitude of P1 elicted by novelty stimulus were larger in eye movement training group( (6.78 ± 1.55 ) μV) and in finger training group ( ( 5.43 ± 1.47 ) μV) than untreated control group ( ( 3.09 ± 0.98 ) μV) significantly, especially in the frontal area. The amplitude of P1 elicted by target stimulus were larger in eye movement training group( (6.75 ±2.01 ) μV)than in finger training group( (4.12 ± 1.33 )μV)and untreated control group( (3.45 ± 1.01 )μV)significantly, especially in the frontal area. The amplitude of P3a were larger in eye movement training group( ( 10. 19 ± 3.09)μV ) than in finger training group ( ( 7.57 ± 2.66 ) μV ) and untreated control group ( ( 6.06 ± 2.03 ) μV ) (P ＜ 0.05,P＜0.05) significantly,especially in the frontal area. The latency of P3a were earlier in eye movement training group( (390.67 ±55.03 ) ms) compared to finger training group( (428.55 ± 48.68 ) ms) and untreated control group( (435.89 ± 59.21 )ms)significantly, especially in the frontal region and central parietal area. Conclusion Eyes movement can improve the MCI patients' non-selective attention function, especially in frontal area.The finger execrises have no significant effect on visual attention.

Objective To investigate the efficacy and influence factors of hysteroscopic guiding endometrial resection on dysfunctional uterine bleeding.Methods One hundred and eighty women with dysfunctional uterine bleeding were selected as our subjects in Maternal and Child Health Hospital of Qinhuangdao from Jan.2009 to Jan.2011.One hundred and fifty which meet with operation indications underwent hysteroscopic guiding endometrial resection.Operation periods,blood loss,adverse effects rate were recorded.Serum follicle stimulating hormone (FSH),luteinizing hormone (LH),estradiol (E2) and progesterone (P) hormone levels were detected at preoperative and postoperative.The efficacy was recorded at 6 and 12 months after the procedure.Results The average operation time and amount of bleeding were (31.2 ± 11.0)min and (32.3 ± 10.5) ml,and no blood transfusion performed during the operation.All cases got the succeed operation.There were no significant difference in terms of serum FSH,LH,E2 and P concentrations before and after treatment(P ＞0.05).At 6 months after treatment,improvement rate was 64.0％ (96/150),and effective rate was 32.0％ (48/150).The total effective rate was 96.0％ (144/150).At 12 months after treatment,the improvement rate was 58.0％ (87/150),effective rate was 36.0％ (54/150).The total effective rate was 94.0％(141/150).There was significant different in terms of total effective rate between 6 months and 12 months after treatment(x2 =113.6,P ＞ 0.05).Postoperative complications occurred in 2 cases,including 1 cases of abdominal pain,and 1 cases of dysmenorrhea.Uterine cavity depth,age and disease duration were the main factors affect the clinical efficacy.(OR(95 ％ CI):-1.14 (-1.17 to-1.07),1.60 (1.31 to 2.08),-1.02(-1.14 to-1.08),P ＜ 0.05).Conclusion Treatment of hysteroscopic guiding endometrial resection on dysfunctional uterine bleeding is proved to be efficacy due to the less adverse reaction.

Objective To discuss the expression and clinical significance of vascular endothelial growth factor (VEGF)-C and nm23-H1 in colorectal carcinoma.Methods Immunohistochemical staining was employed to determine the expression of VEGF-C and nm23-H1 in the carcinoma tissues of 120 cases with colorectal carcinoma and in the adjacent mucosal tissues of 55 cases as control,and analyzed their correlation with cliniopathological features and prognosis.Results The positive expression of VEGF-C in the carcinoma tissues was 71.7％ (86/120),significantly higher than that in the adjacent mucosal tissues [21.8 ％ (12/55)],and there was significant difference (x2 =35.186,P ＜ 0.01).The positive expression of nm23-H1 in the carcinoma tissues was 57.5％ (69/120),significantly lower than that in the adjacent mucosal tissues [90.9％ (50/55)],and there was significant difference (x2 =18.351,P ＜ 0.01).The expression of VEGF-C was significantly correlated with lymph node metastasis (P ＜ 0.05),and the expression of nm23-H 1was significantly correlated with lymph node metastasis and pathological type (P ＜0.01 or ＜0.05).The expression of VEGF-C and nm23-H1 did not show a significant correlation with gender,age,tumor size,tumor site and depth of invasion (P ＞ 0.05).Spearman correlation analysis showed that the expression of VEGF-C was negatively correlated with the expression of nm23-H 1 in colorectal carcinoma (r =-0.472,P ＜0.01).Conclusions The joint detection of VEGF-C and nm23-H1 expression is very promising in prediction of the prognosis of patients with colorectal carcinoma.However,whether it can be used as a marker in prognosis judgment need further investigation.

Objective To explore the effect of nicotine on the degeneration of dopaminergic neurons in PD rats. Methods Forty-five SD rats were randomly divided into three groups: PBS group (CON), normal saline + LPS ( NS ) group and nicotine +LPS ( NIC ) group. On 24 hours after LPS or PBS injection, inducible nitric oxide synthase ( iNOS ) protein expression was examined by immunoblotting. ON 14d after LPS or PBS injection, the numbers of tyrosine hydroxylase ( TH ) positive neurons and morphological changes of OX-42 positive cells in the substantia nigra(SN) were observed by immunohistochemistry. TH mRNA and TH protein expressions were examined by RT-PCR or immunoblotting. Results Compared with CON group, iNOS protein increased markedly 24 hours after LPS injection in NS group. TH positive neurons, TH mRNA and TH protein in the SN decreased remarkably 14 days after nigrainjiection. Most of microlglial showed big cell body with thicker and shorter processes. However, nicotine reversed the above changes, Compared with NS group, TH positive neurons, TH mRNA and TH protein in the SN increased remarkably in NIC. Besides, most microglia showed small cell body with slim and long processes. Conclusion Nicotine could prevent LPS-induced degeneration of DA neurons, probably because of that the pretreatment with nicotine blocks the activation of microglia and the expression iNOS protein.

0.05). But the level of HbA1c, 2 hour insulin and C peptide were significantly lower than that in CG (P 0.05). Conclusion Wei li acupuncture therapy instrument can improvement the islet ?-cell function in patients with type 2 diabetes mellitus.

Objective To investigate expression of elderly patients with endometrial cancer of estrogen receptor and progesterone receptor and its clinical significance.Methods 45 elderly patients with endometrial carcinoma from March 2012 to June 2014 were selected as the observation group, over the same period healthy women with normal endometrium as control group, using S-P immunohistochemical method, to detect the expression of receptor and progesterone receptor in two groups.Results In observation group, estrogen and progesterone receptor positive expression rates were 60.00%, 46.67%, compared with control group(93.33%, 86.67%), was significantly lower (P<0.05).Patients with low differentiation of estrogen and progesterone receptor, positive expression rate was 37.50%, 25.00%, was lower than other two groups, but which had no difference.Estrogen, progesterone receptor positive expression rates in progress stage were 35.71%, compared with patients ( 80.65%, 74.19%) in early stage, was significantly lower (P<0.05).The positive expression rate of estrogen receptor in patients with lymph node metastasis was 66.67%, compared with the patients with no metastasis, the difference was not statistically significant.The positive expression rate of progesterone receptor was 76.67%, compared with the non metastasis group (46.67%), and was significantly lower (P<0.05).Conclusion The expression of estrogen and progesterone can reflect the biological behavior and prognosis of endometrial carcinoma in aged patients, and have important significance for clinical diagnosis and treatment.