1.PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.
Xiaona HUANG ; Chao WEI ; Fenjie LI ; Lumeng JIA ; Pengguihang ZENG ; Jiahe LI ; Jin TAN ; Tuanfeng SUN ; Shaoshuai JIANG ; Jia WANG ; Xiuxiao TANG ; Qingquan ZHAO ; Bin LIU ; Limin RONG ; Cheng LI ; Junjun DING
Protein & Cell 2019;10(10):709-725
Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression. However, the mechanism underlying the activation function of PCGF6 is poorly understood. Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4. In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression. By combining with promoter capture Hi-C data, we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D chromatin. Our findings highlight a novel mechanism of PcG protein in regulating pluripotency, and provide a research basis for the therapeutic application of pluripotent stem cells.
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