1.Pathologic staining method improvement for detection of pulmonary cryptococcal disease
Xiuqin WENG ; Shijuan YANG ; Zhisheng XIANG ; Gang CHEN
Cancer Research and Clinic 2017;29(12):841-843
Objective To investigate the pathological staining method of pulmonary cryptococcal disease. Methods Traditional hexamine silver staining and acidified potassium permanganate solution hexamine silver stained were used to analyze paraffin sections of 51 patients with pulmonary cryptococcal disease. Results Compared with traditional hexamine silver stained, the result of acidified potassium permanganate solution with hexamine silver stained was positive, and the whole process only required 25 minutes with the clear background and cryptococcal cyst capsule structure. Conclusion Compared with the traditional hexamine silver staining, acidification of potassium permanganate solution hexamine silver staining method is simpler,easier,more effective and worthy of wide application.
2.Chemotherapy initiation with single-course methotrexate alone or combined with dactinomycin versus multi-course methotrexate for low-risk gestational trophoblastic neoplasia: a multi-centric randomized clinical trial.
Lili CHEN ; Ling XI ; Jie JIANG ; Rutie YIN ; Pengpeng QU ; Xiuqin LI ; Xiaoyun WAN ; Yaxia CHEN ; Dongxiao HU ; Yuyan MAO ; Zimin PAN ; Xiaodong CHENG ; Xinyu WANG ; Qingli LI ; Danhui WENG ; Xi ZHANG ; Hong ZHANG ; Quanhong PING ; Xiaomei LIU ; Xing XIE ; Beihua KONG ; Ding MA ; Weiguo LU
Frontiers of Medicine 2022;16(2):276-284
We aimed to evaluate the effectiveness and safety of single-course initial regimens in patients with low-risk gestational trophoblastic neoplasia (GTN). In this trial (NCT01823315), 276 patients were analyzed. Patients were allocated to three initiated regimens: single-course methotrexate (MTX), single-course MTX + dactinomycin (ACTD), and multi-course MTX (control arm). The primary endpoint was the complete remission (CR) rate by initial drug(s). The primary CR rate was 64.4% with multi-course MTX in the control arm. For the single-course MTX arm, the CR rate was 35.8% by one course; it increased to 59.3% after subsequent multi-course MTX, with non-inferiority to the control (difference -5.1%,95% confidence interval (CI) -19.4% to 9.2%, P = 0.014). After further treatment with multi-course ACTD, the CR rate (93.3%) was similar to that of the control (95.2%, P = 0.577). For the single-course MTX + ACTD arm, the CR rate was 46.7% by one course, which increased to 89.1% after subsequent multi-course, with non-inferiority (difference 24.7%, 95% CI 12.8%-36.6%, P < 0.001) to the control. It was similar to the CR rate by MTX and further ACTD in the control arm (89.1% vs. 95.2%, P =0.135). Four patients experienced recurrence, with no death, during the 2-year follow-up. We demonstrated that chemotherapy initiation with single-course MTX may be an alternative regimen for patients with low-risk GTN.
Antineoplastic Combined Chemotherapy Protocols/adverse effects*
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Dactinomycin/adverse effects*
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Female
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Gestational Trophoblastic Disease/drug therapy*
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Humans
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Methotrexate/therapeutic use*
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Pregnancy
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Retrospective Studies