Objective To study the expression of CD44V6 in human epidermal tumours and malignant melanoma. Methods The expression of CD44V6 was detected by an immunohistochemical technique in squamous cell carcinoma(SCC), basal cell carcinoma(BCC) and malignant melanoma(MM). Results CD44V6 was expressed on the membrane of tumour cells of 10 patients with SCC, and there was downregulation of CD44V6 expression which along with the decrease of differentiation of SCC tumour cells. Tumour cells of 8 patients with MM and 10 patients with BCC did not express CD44V6. Conclusion Our findings suggest that CD44V6 expression is correlated with the types of skin tumours.

33 cases of pain due to neural - root type cervical spondylosis were treated by Bitongxiao. with another 31 cases treated by Jingfukang granule as control. The pain scores in both groups before and after the treatment were markedly decreased (all P

AIM: The aim of this investigation was to confirm the best operation conditions in the membrane separation of protein from the homogenate of red earthworm, Eisenia faefida. METHODS: Ultrafiltration membrane of fresh red earthworm with the cut-off of 10 000 was used as the target process, with the homogenate flux as index, the best value of operation parameters such as pressure、 temperature, surface rotation speed and feed concentration were fixed on. RESULTS: (1) The best suitable operation conditions were 0.25 MPa, 30?C , 200 r/min, pH=6.7, 33.3% (w:w) concentration and not a bit of NaCl. (2) The molecular weight distribution of protein in the homogenate of red earthworm mainly lay between 50 000～100 000. CONCLUSION: The obtained experimental results will provide pilot scale test with data support.

Objective To explore the effect of rehabilitation on pneumonia in children with cerebral palsy (CP).Methods60 CP children with pneumonia were randomly divided into the control group and rehabilitation group with 30 cases in each group. All children in two groups received active support therapy but those in the rehabilitation group were added with rehabilitation treatment.ResultsThe time of body temperature decline, alleviation of cough and expectoration, role disappear of the rehabilitation group were shortened compared with that of the control group ( P<0.05).ConclusionRehabilitation treatment is helpful for treatment of pneumonia in CP children.

Plasma endothelin-1 (ET-1),von Willebrand factor ( vWF) and fibrin D-dimer (D-D) were determined in 14 children with schnlein-henoch purpuric nephritis (SHN),17 children with schnlein-hen och purp ura (SHP, no abnormal finding in the examination of the urinary system) and 12 children as normal controls, respectively. The results showed that plasma ET-1( 88.48±22.96ng/L),vWF(1.59±0.38U/ml) and D-D(1.45±0.39)mg/L in the SH N group were all significantly higher than those in both of the control group (4 3.73±17.89)ng/L, (0.99±0.3)U/ml and (0.28±0.23)mg/L and the SHP group (57. 54±20.92)ng/L, (1.5±0.31)U/ml and (0.64±0.34)mg/L although no significa ntly difference in the levels of vWF was observed between SHN and SHP groups. It was noticed that the levels of three parameters decreased significantly in the SHN g roup after treatment (all P＜0.01). There was a positive correlati on between the levels of plasma ET-1 and D-D with serum creatinine (all P＜0.01). It is suggested that excessive ET-1 induced by endothelial dam age of renal vessels, intravascular coagulation and secondary fibrinolysis are p robably involved in the process of renal damage.

Purpose Two kinds of forward conditional reasoning including modus ponens (MP) and denial of the antecedent (DA) are the most common deductive reasoning in daily life. The present study aimed to explore the difference between the two neural machanisms. Materials and Methods Three kinds of tasks including MP, DA, and baseline were administrated to 14 undergraduates [7 males and 7 females, mean age (23.4±1.3) years] by using functional magnetic resonance imaging (fMRI) technique. fMRI data was analyzed. Results The reaction times were significantly slower for MP and DA than that of BS (P<0.001), while the effect was not significant for accuracy. In contrast to baseline, both MP and DA tasks activated the left inferior frontal cortex (BA 9), inferior parietal lobule (BA 40), and postcentral gyrus. Bilateral caudate significant activation was found in MP task when compared with DA, whereas the frontal, parietal, occipital lobe and anterior cingulate cortex were acivated more in the DA than in MP task. Conclusion Both forward conditional reasoning of MP and DA commonly activate the left frontoparietal cortex, while significant dissociations can be seen in the regions of caudate and bilateral frontal, parietal and occipital. These results firstly demonstrates the different neural mechanism underlying the two forward conditional reasoning, which might help to further uncover the brain mechanism of conditional reasoning.

Objective To investigate the best exposure condition for reducing the radiation dose of the chest radiography and obtaining the good quality of images. Methods With different tube voltage (kVp), tube current & time (mAs), and focus-film distance(FFD) adopted, X-ray detecting phantom was exposed by means of automatic exposure control(AEC). The dosage absorbed in skin was detected; Spatial resolution capacity, low contrast resolution capacity and low contrast sensitive degree were tested. The space and density resolution capacity were tested. The chest image quality was appraised by the way of visual observation and the measure of film density. Results The different kVp and FFD were related to the emitting skin dose (ESD), the higher kVp and the farther FFD follows the lower ESD; the higher or lower mAs has no effect on the image resolution capacity , but it had effect on image noise. The density value and seeing effect of chest film with low dose had reached good image density and needs of diagnostics. Conclusion Under the condition of 120 kVp high voltage, FFD150cm, AEC reduced density and adjusting to the processing parameters of the CR chest images, the radiation dosage of the chest computed radiography can be reduced efficiently and the good quality of images can be obtained.

Objective To study influencing factors in patients with rheumatic diseases.Methods One hundred and twenty patients with rheumatic diseases were selected from November 2013 to February 2014 in our hospital for the study.The influencing factors in patients with rheumatic diseases were obtained by proposed questionnaires,and then the univariate and multivariate Logistic regression analysis were performed to study factors affecting the treatment.Results Patients older than 50 of the observation group accounted for 38％ (18/48),the proportion of farmer,patients with primary school education level or below,and monthly income ＜3 000 RMB was 35％(17/48),60％(29/48),and 46％ (22/48) respectively.Compared with the control group,they were significantly higher than the control group,which were 64％(46/72),58％ (42/72),79％(57/72),65％(47/72) (x2=8.058,P=0.005; x2=6.025,P=0.014; x2=4.986,P=0.226; x2=4.456,P=0.035) respectively.Using the treatment as the dependent variable,age ≥50 years of age,farmer,primary school and lower education level,and monthly income ＜3 000 RMB as the independent variable,we carried out the regression analysis.Age (OR=1.124,95％CI:1.084-7.236),occupation (OR=1.871,95％CI:1.054-7.243),education level (OR=1.982,95％CI:1.157-6.256),monthly income (OR=1.363,95％CI:1.012-8.227) are the influencing factors of rheumatism's treatment.Conclusion The treatment of rheumatic diseases are influenced by many factors,and more societal support should be provided to help patients with rheumatic disease receive professional treatment and better control of the disease.

Objective To detemine the value of cardiac troponin in early diagnosis of severe myocardial contusion in the dog.Methods Twelve dogs weighing (11.4 ± 1.5) kg were subjected to severe myocardial contusion by impacting the chest area with BIM-Ⅱ biological impact machine.Electrocardiogram,cTnT and cTnI were measured before,immediately,and 2,4,6 and 8 hours after injury.Animals were then killed and the serum was separated for gross examination and triphenyl tetrazolium chloride (TTC) staining.Results Supraventricular tachycardia,ventricular tachycardia,ventricular premature beat,myocardial ischemia,atrial fibrillation,and ventricular fibrillation were seen on the electrocardiogram 2,4,6 and 8 hours after the injury,which suggested a high sensitivity but low specificity.cTnT and cTnI levels revealed no specific changes at postoperative 2 and 4 hours,but cTnT and cTnl were significantly increased to (0.130 ± 0.052) ng/ml and (1.615 ± 0.371) ng/ml at postoperative 8 hours,significantly higher than that immediately after operation (P ＜ 0.01).Sensitivity and specificity of cTnT and cTnI were both 100％,while the specificity of the TTC staining was (39.78 ± 9.07)％.Conclusion Cardiac troponin is of high sensitivity and specificity in early diagnosis of severe myocardial contusion and has good correlation with pathological changes,which exhibits great potential in clinical application.

Objective To observe the expression of Toll-like receptor(TLR) on peripheral blood dendritic cells(DC) in children with Henoch-Schtinlein purpura(HSP),and to investigate the pathogenesis of the abnormal expression of TLR in children with HSP.Methods Twenty hospitalized children with HSP in the Affiliated Hospital of Qingdao University Medical College from Dec.2011 to Jul.2012 were enrolled in the study(HSP group).Twenty agemetched healthy children were selected as a healthy control group.Peripheral venous blood was sampled under aseptic condition,peripheral blood mononuclear cells (PBMC) were isolated from density gradient centrifugation,and DC were generated by recombinat human granulocyte-macrophage colony-stimulating factor(GM-CSF),interleukin-4(IL-4) and tumor necrosis factor-α(TNF-α) in vitro.Expressions of CD83,CD86 and TLR2,TLR3,TLR4 in peripheral blood DC were examined by fluorescent activated cell sorter (FACS).Results 1.No significant distinction was found in the expression of the C Ds3 on peripheral blood DC between HSP group and healthy control group(t =0.80,P ＞ 0.05) ;in HSP group had remarkably increased expression of the CD86 on peripheral blood DC than that of the healthy control group (t =9.56,P ＜ 0.01).2.Expression rates of TLR2,TLR3,TLR4 on peripheral blood DC in the HSP group were higher than those in the healthy control group(t =1 1.79,13.29,9.45,all P ＜ 0.01).3.Expression rates of TLR2,TLR3 and TLR4 in HSP group had positive correlation with expression rates of CD86 (r =0.84,P ＜ 0.01 ; r =0.53,P ＜ 0.05 ; r =0.66,P ＜ 0.05).Conclusions Expressions of TLR2,TLR3 and TLR4 on peripheral blood DC significantly increased and were positively correlated with expression of CD86.This implies that TLR and co-stimulatory molecules might participate in the pathogenesis of HSP by mediating signal transduction,leading to abnormity of cytokines,then inducing Th1/Th2 immune imbalance by showing the advantage of Th2 function.