1.Research in effect of modified drug preparation method on reduction of air bubble formation in infusion bags
Chinese Journal of Practical Nursing 2011;27(3):53-54
Objective To investigate a new method to reduce air bubbles formation during drug preparation. Methods 200 portions of drugs from hospital pharmacy were randomly divided into the control group and the experimental group with 100 portions in each group. In the experimental group, the infusion bags were put flat on the desktop, the needles pierced into the bags parallel, and immersed in the liquid inside. In the control group, the infusion bags were put perpendicular to the desktop, the needle pierced into the bags vertically, the needles do not immersed in the liquid. Air bubble formation in the infusion bags was compared between the two groups. Results Air bubble formation in the experimental group was significantly less than that of the control group. Conclusions The modified new method for drug preparation can reduce the formation of air bubbles.
2.The role of adrenomedullin in diabetic nephropathy
Huimin LI ; Heng MIAO ; Xiuqin JIANG
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To investigate the role of adrenomedullin (AM) in diabetic nephropathy. METHODS: We observed the changes in the expression and secretion of AM, TGF-? 1 in the cultured human mesangial cells under high glucose condition and the contents of the laminin and type IV collagen in the supernatants. The effect of intervention with AM was also observed. RESULTS: High glucose condition resulted in increase in the expression and secretion of AM TGF-? 1 laminin and type IV collagen. AM reversed the influence of high glucose on the cultured human mesangial cells. CONCLUSION: These results showed that high glucose condition is one of stimulating factors of AM and the renal protective action of AM may be associated with suppression of TGF-? 1 and reducing excessive accumulation of laminin and type IV collagen.
3.Reform of medical genetics teaching
Zequn JIANG ; Fengming ZHAO ; Xiuqin ZHAN ; Mingyan WANG
Chinese Journal of Medical Education Research 2012;11(9):937-939
Medical genetics is the mutual penetrative and combined discipline of genetics and medicine.Medical genetics teaching should be improved to adapt to the discipline development.Teaching reform and practice of medical genetics was developed in basic medical college of Nanjing university of traditional Chinese medicine.Constructive exploration was made in teaching content,teaching techniques,teaching methods,scientific research and performance appraisal standards in order to improve the quality of medical genetics teaching and to train high-quality medical talents.
4.Rehabilitation of aphasia after cerebral infarction using repetitive transcranial magnetic stimulation and donepezil
Shengdong CHEN ; Suwen YU ; Jianfa ZHAO ; Xiuqin ZHEN ; Jiangbo JIANG
Chinese Journal of Physical Medicine and Rehabilitation 2012;34(3):212-215
Objective To evaluate the effectiveness of using low-frequency repetitive transcranial magnetic stimulation (rTMS) along with donepezil in the rehabilitation of aphasia after cerebral infarction. Methods Fortythree aphasic patients with acute cerebral infarction were assigned into a treatment group or a control group.Both groups received standard basic treatment for acute cerebral infarction.From day 7 on,both groups received oral donepezil hydrochloride (5 mg/d) for 12 consecutive weeks.The treatment group received in addition 2 sessions of low frequency rTMS on the language center of the right hemisphere.The first session started at day 7 after onset,and each session consisted of rTMS on 10 consecutive days (once per day).There was a resting interval of 3 d between the two sessions.The rehabilitation of the aphasia was assessed prior to and after each session,and at the end of the entire 12 weeks of treatment using the western aphasia battery ( WAB ) scoring system and the aphasia quotient (AQ). Results There was no significant difference between the two groups after the first session in terms of the WAB or AQ results.However,the difference in improvement became statistically significant after the second session,as well as at the end of the entire 12 weeks.The average change in AQ was significantly greater in the treatment group. Conclusion Donepezil combined with rTMS early in the acute phase of cerebral infarction is helpful for the rehabilitation of aphasia.
5.Treating Parkinson's disease with high and low frequency repetitive transcranial magnetic stimulation: a clinical study
Xiuqin ZHENG ; Suwen YU ; Shengdong CHEN ; Jianbo JIANG
Chinese Journal of Physical Medicine and Rehabilitation 2012;(12):907-910
Objective To investigate the therapeutic effects of repeated transcranial magnetic stimulation (rTMS) at high and low frequency for the rigidity and tremors of Parkinson's disease (PD).Methods Sixty-nine patients with PD received rTMS at 5 Hz or 0.5 Hz.The efficacy was assessed using the unified Parkinson's disease rating scale (UPDRS),motor function tests and motor evoked potentials (MEPs).Results Thirty-two patients with rigidity from PD received 5 Hz rTMS therapy,and their UPDRS total scores decreased significantly.Their motor function test results improved significantly and their MEP relaxed motor thresholds were significantly elevated.Thirtyseven patients with tremors from PD received 0.5 Hz rTMS therapy,and their UPDRS total scores were significantly decreased.Their motor function test results improved significantly and their MEP relaxed motor thresholds were significantly elevated.Conclusion High frequency rTMS may improve the symptoms of patients with PD rigidity and low frequency rTMS may improve the symptoms of patients with PD tremors.
6.Study on optimization of operation parameters of microfiltration separation of effective matters in earthworm homogenate using inorganic ceramic membrane
Qigang DAI ; Weicheng JIN ; Pei JIANG ; Xiuqin ZHAN
Chinese Traditional Patent Medicine 1992;0(05):-
AIM: To aim to seek the best operation conditions of the inorganic ceramic membrane in the separation of effective matters from the homogenate of earthworm. METHODS: The homogenate flux was adopted as index,to select the membrane material,filter pressure、homogenate temperature and concentration. RESULTS: The experiment showed that the filter flux had a significant positive correlation with pressure difference and liquid temperature,and negative correlation with liquid concentration. CONCLUSION: In the separation of earthworm homogenate,0.2 ?m Al_2O_3 and 50 nm ZrO_2 film pipe were respectively selected as primary and secondary separating unit on the condition of 0.15 mPa pressure,50 ℃ and 33.%(w/w) homogenate concentration.
7.Experimental studies on fibronectin expression in myocardium taken from rats died of electrocution
Guisheng OU ; Hualan JING ; Fuxue JIANG ; Yanrong LI ; Xiuqin XIE ;
Chinese Journal of Forensic Medicine 2002;0(05):-
Objective To establish an immunohistochemical method for postmortem diagnosis of electrocution. Method 12 rats were divided into two groups,experimental and control. In the experimental group,6 rats were killed by electricity,and in the control group,6 rats were killed by breaking the rats' neck. Myocardium sections were then made and were stained by SABC immunohistochemical method as well as HE.Results The expression of the fibronectins in the experimental rats' myocardia were significantly increased as compared with those in the control rats (P
8.Effect of valsartan on the expressions of Toll-like receptor 4 and myeloid differentiation factor 88 in the arteries of diabetic rats
Jun WU ; Na YOU ; Jiarong XU ; Zhihua ZUO ; Aiping HU ; Xiuqin JIANG ; Heng MIAO ; Yibing LU
Chinese Journal of Endocrinology and Metabolism 2009;25(4):378-381
ay be useful for preventing or treating early inflammation in the arteria of diabetic rats.
9.Comparison of X gene Sequences of Hepatitis B Virus Isolates in Hepatitis B Induced Liver Cirrhosis Patients and HBV Carriers
Jianwei SHEN ; Xu WANG ; Shuangyi LI ; Wentao JIANG ; Lihong TIAN ; Xiuqin SUN ; Yumei WAN
Chinese Journal of Nosocomiology 2006;0(09):-
OBJECTIVE To discover diversity of X gene sequences of hepatitis B virus isolates in hepatitis B induced liver cirrhosis patients and HBV carriers.METHODS DNA fragment including X gene sequences of hepatitis B virus was amplified,sequencing PCR products was preformed.The PCR products of three liver cirrhosis patients and three HBV symptomless carriers were cloned into pGEMT Easy vectors.Positive clones with target sequences were selected out for sequencing.Sequence comparison was made to find the identity.RESULTS A comparison of T1762/A1764,G1719T,T1727G/A,G1730C and T1753C mutations in a core promoter between the liver cirrhosis patients and the HBV carriers showed that the HBV isolates from the former had higher frequencies of mutation than the latter.The X promoter region of the HBV isolates from the liver cirrhosis patients showed higher frequencies of mutation than the isolates from the HBV carriers.Additionally,the homology between clones of X gene from one individual with liver cirrhosis averages 91.3-99.7%,that of HBV carriers averages 96-100%.CONCLUSIONS The core and X promoter region of the HBV isolates from the liver cirrhosis patients show the higher frequencies of mutation than the isolates from the HBV carriers.There are HBV quasispecies which possess great variation in the liver cirrhosis patients.
10.Effects of arsenic trioxide on hepatoma cell line HLE
Lianxin LIU ; Hongchi JIANG ; Anlong ZHU ; Jing ZHOU ; Xiuqin WANG ; Min WU
Chinese Journal of General Surgery 2001;10(2):134-138
Objective To observe the effect of various concentration of arsenic trioxide (AT) on hepatoma cell line HLE in variable duration. Methods The cell activity, morphologic changes and apoptosis were studied after being treated with different concentration of AT. Results The effect of AT on hepatoma cell lines was depending on the time and concentration obviously. Hepatoma cells cultured with different concentration of AT presented apoptosis features, such as intact cell membrane, chromatin condesation, nucleic fragmentation and apoptotic body formation; flow cytometry analysis showed that an arrestment at G2/M phase, an apoptosis peak presented before sub-G1 cell peak, and a mark DNA ladder of apoptosis appeared. Conclusions AT can obviously inhibit the growth of hepatoma cell through inducing hepatoma cell apoptosis.