Acupuncture Therapy ; Cerebral Hemorrhage ; complications ; Cerebral Infarction ; etiology ; therapy ; Humans ; Male ; Middle Aged

Objective To observe the effect of auricular point sticking plus breast massage with rehabilitation apparatus on lactation in the puerperant. Methods Totally 127 in-hospital primiparae were randomized into a treatment group of 62 cases and a control group of 65 cases. The control group was intervened by conventional nursing care, while the treatment group was by auricular point sticking plus breast massage with rehabilitation apparatus in addition to the conventional nursing care. The lactation yield, initial lactation time, and the change of prolactin (PRL) were observed. Results There were significant differences in comparing the lactation yield grading and initial lactation time between the two groups (P＜0.05). There were significant differences in comparing the serum PRL level on the postpartum day 1, 2, and 5 between the two groups (P＜0.01, P＜0.05). Conclusion Auricular point sticking plus breast massage with rehabilitation apparatus can promote milk secretion, the initial lactation time, PRL production, and the breast feeding rate.

Objective To analyze the morphological matching rate with a digital image recognition system in pre-and post-orthodontic treatments,and to provide forensic dentistry evidence for individual identification.Methods High-resolution digital images of the palatal rugae were taken under particular conditions from each patient in pre-and post-orthodontic treatments.Features of each digital image were extracted using a digital image recognition system and collected in the palatal rugae database.Data of the palatal rugae werematched by MATLAB software system.Results From the matching results,the accuracy rate of individual identification of orthodontic patients was 100％.The accuracy rate in matching palatal rugae patterns in pre-and post-orthodontic treatments was 95.67％.The results were statistically significant with P＜0.05.Conclusion Palatal rugae pattern is highly individual-specific.The morphologyofpalatal rugaecould bechangedby theorthodontic treatment,however,these changes may not greatly influence individual identification in forensic dentistry,providing a new potential to individual identification.

Background Ostrich acellular corneal stroma possesses a similar constitution to human corneal stroma,so it is expected to become one of ideal biological corneal carriers.Objective This study was to investigate the immunogenicity of acellular stroma carrier of ostrich cornea and offer the information for the development of industrialization and clinical use of acellular stroma carrier of ostrich cornea.Methods Twenty fresh ostrich eyeballs and 20 porcine eyeballs were collected.Acellular corneal stroma carriers of ostriches and pigs were prepared using low temperature freezing joint enzyme digestion method and desiccant dehydration method and sterilized by cobalt-60 irradiation.The corneal stroma carriers were preserved using drying and dehydration method.Forty-five male BALB/c mice were randomly divided into the sham operation group,ostrich acellular corneal stroma group and porcine acellular corneal stroma group.Acellular corneal stroma carriers of ostriches and pigs(wet weight after rewatering was 10 mg/piece) were subcutaneously implanted to the back of BALB/c mice,respectively.Wound healing and inflammatory response on the operative site were observed,and phenotype and activating rate of CD4+,CD8+ and CD25+in peripheral blood of mice were dynamically detected 7,14 and 28 days after ectopically implantation of heterogeneous corneal stroma by immunofluorescence labeling and flow cytometry analysis.Results No swelling and exudation were seen in the skin of operative site of the mice with a good healing of wound after surgery.There were no significant differences in the activating rates of CD4+,CD8+ and CD25+ cells in the peripheral blood of mice among the sham operation group,the porcine acellular corneal stroma group and ostrich acellular corneal stroma group in the three time points after surgery(CD4+:F=0.74,P=0.50;F=0.39,P=0.05;F=3.46,P=0.58.CD8+:F=1.75,P=0.21 ;F=1.14,P=0.35;F=0.78,P=0.48.CD25+:F=0.52,P=0.61 ;F=3.53,P=0.62;F=2.42,P=0.13).Conclusions The ostrich acellular heterogeneous corneal stroma carrier possesses low immunogenicity.It is inferred that ostrich acellular corneal stroma carrier can be used in heterogeneous corneal transplantation.

Objective To select the largest non-toxic leaching solution concentration through the experimental observation of the cytotoxicity of the ostrich acellular corneal stromal leaching solution to the Chinese hamster lung fibroblasts cells(CHL) for the further chromosome distortion experiment.Methods The leaching solution made from the ostrich acellular corneal stromal material was diluted with concentrate of 1 ∶ 2,1 ∶ 4 and the original concentration were used to culture with the CHL cells,the negative and positive control were also set up at the same time,to evaluate the impact on cell growth after 24 hour by MTT colorimetric method.Results The leaching solution diluted with 1∶4 was non-toxic,and could promote the growth of the cells.Conclusion Combined with the results of classification and cell morphological features,this cytotoxicity test can be used to screen the best benchmark non-toxic concentrations for the chromosome aberration test of the CHL cells.

Objective To establish a digital system for forensic identiifcation of the palatal rugae and evaluate its application effects. Methods High-resolution digital images of the palatal rugae were harvested under the standard condition and processed including data collection, noise reduction, contrast enhancement, image segmentation, feature extraction, edge detection, and information matching. Apply an MATLAB software system to develop a digital system for forensic identiifcation of the palatal rugae ,and its application effects were evaluated. Results A digital system for forensic identification of the palatal rugae was successfully established. The digital system had an accuracy rate of 100%. Conclusion Establishing a digital system for forensic identiifcation of the palatal rugae provides a novel method for forensic identiifcation.

Objective To investigate the changes of peripheral insulin resistance after lipid infusion and the effect of N-acetylcystein(NAC) intervention.Methods Thirty-seven normal male SD rats,eight weeks old,were randomly divided into three groups,FFA group,NS group and NAC group(using into NAC 300 mg/(kg?d) two weeks before infusion).Catheters were implanted into right atrium via the jugular vein and left carotid artery.A technique for a 48-h infusion in unrestrained rats was used for triglyceride and heparin or saline infusion.The infusion period started on day 2 after surgery.48-h after infusion,we determined free fat acid(FFA),nitrotyrosine,malonaldehyde(MDA),reduced glutathione hormone(GSH) level in plasma.The glucose infusion rat(GIR) was measured by hyperinsulinemia euglycemic clamp to evaluated the perpherial insulin resistance.The expressions of IRS-1,IRS-2 gene in muscle were detected by real time PCR.Results(1)The FFA,nitrotyrosine and MDA con-centrations in FFA group were higher than that in NS group,but GSH level in plasma was lower.NAC intervention could reverse these effects.(2)GIR was decreased significantly in FFA group as compared with NS group[(8.34?1.8)mg/(min?kg)] vs[(13.56?1.7)mg/(min?kg)],(P

Objective To evaluate the clinical value of ultrasonography in the diagnosis of hermaphroditism.Methods Ultrasonographic appearances of 45 cases with hermaphroditism were reviewed retrospectively,and the results were compared with clinical data.Results All cases were confirmed pathologically.5 cases showed true hermaphroditism (11.1％),14 cases showed male pseudohermaphroditism (31.1 ％),24 cases showed female pseudohermaphroditism (53.3 ％),2 cases showed gonadal dysgenesis(4.4％).Compared with pathological results in the ultrasound examination,4 cases showed true hermaphroditism,the coincidence rate was 80％.11 cases showed male pseudohermaphroditism,the coincidence rate was 78.6％.20 cases showed female pseudohermaphroditism,the coincidence rate was 83.3％.1 cases showed gonadal dysgenesis,the coincidence rate was 50.0％.Conclusions Ultrasonography can not only evaluate internal reproductive organs in the patients,but also estimate the site,size,morphology and structure,which provides important imaging evidence for the clinical diagnosis and treatment.

Objective To evaluate the palatal rugae individual-specific properties and stability before and after orthodontic treatments for investigating the reliability of palatal rugae for individual identification of forensic dentistry based on the palatal rugae model ananlysis. Methods Maxillary models of 70 patients were obtained before and after orthodontic treatments respectively. Palatal rugae morphologic changes and individual identification were examined by model analysis including changes in number, orientation, shape, and length of palatal rugae, as well as mesial and distal endpoint displacement in the anterioposterior(AP) and mesiodistal (MD) direction. Furthermore, The examination of palatal rugae average match accuracy(%) was performed between the pre-treatment and post-treatment orthodontics models and post-treatment and duplicated post-treatment orthodontics models. Results ① The palatal rugae morphological changes were found and the incidence of palatal rugae changes were showed in length(28.6%), segmenta-tion(4.3%); unification(2.9%); orientation(1.4%) and shape(1.4%) after orthodontic treatment. Also the displacement of the distal endpoint of palatal rugae was 45.7% for anterioposterior indirection, 40% for mesiodistal indirection after orthodontic treatment, while the anterioposterior displacement of the mesial endpoint of palatal rugae was 32.9% and mesiodistal displacement was 17.1%. ② The identical palatal rugae patterns were not found both the pre-treatment and post-treatment groups. ③ The palatal rugae average match accuracy was 92.19% between pre-treatment and post-treatment groups, however, the rate had increased by 99.05% when matching the palatal rugae patterns of post-treatment groups to their duplicates. Conclusion Palatal rugae patterns have the high specific properties to each indivi-dual. Although there are some changes in morphology of palatal rugae after orthodontic treatment, it still can be used as a novel method for individual identification in forensic dentistry.