Objective To observe the curative effect of modified Yiweitang on anorexia of the Spleen and Stomach Yin deficient type. Methods90 patients with anorexia belonging to spleen yin deficiency type were randomized in to 2 groups, by 1: 1 ratio. According to the age of children, appropriate dose of modified Yiwei decoction (treatment group) and Erbao Granule (control group), 3 times/d, for 4 weeks were given to the children. Clinical efficacy of the treatment group was observed before and after the treatment and compared with the control group. Results(①) symptom scores: Vomiting (0.061±0.024), sweating (0.140±0.004), stool (0.024±0.011), urine (0.026±0.010) in treatment group after treatment, showing statistical significance (P＜0.05) with the control group. ② disease treatment: The total effective rate was 93.3％ and 91.1％ in the treatment group and the control group, the difference was not statistically significant (X2=0.886, P＞0.05). ③symptoms treatment: The total effective rate was 95.6％ and 66.7％ in the treatment group and the control group respectively, the difference was statistically significant (x2=0.625, P＜0.05). Conclusion The treatment of modified Yiweitang was better than Erbao Granule in treating children with anorexia belonging to stomach yin deficiency.

Objective To establish a RP- HPLC method for the determinatio n of protocatechualdehyde in Danqi tablet. Methods LiChrospher RP- 18 column w as used, the mobile phase was MeOH- 1.5 % Hac(1∶ 9) with a flow rate of 1.0 mL/ min, and the detection wavelength was at 280nm. Results There was a goo d linearity within the range of 0.030～ 0.150 ? g of protocatechualdehyde. The average recovery was 103.08 % and RSD was 0.796 % (n=5). Conclusion This method can be used for the quality control of Danqi tablet .

Objective To evaluate the association between serum cystatin C and carotid atherosclerosis in patients with type 2 diabetes mellitus.Methods A total of 318 patients with type 2 diabetes mellitus were enrolled in this study.According to the results of carotid color Doppler ultrasound in the patients were randomly divided into two groups:CIMT abnormal group (CIMT was equal to or larger than 0.9 mm,with or without associated with carotid artery plaque formation)CIMT and normal group (CIMT <0.9 mm,and it was not associated with carotid artery atherosclerotic plaque formation.Differences in serum cystatin C,serum lipid and lipoprotein,fasting plasma glucose and other biochemical markers between the two groups were analyzed by using Student's t test.The correlated factors of the carotid thickness and plaque appearance were analyzed by Logistic regression analysis.Results The serum cystatin C levels of the CIMT abnormal group were higher than the normal CIMT group[(1.24 ±0.22)mg/L vs.(1.05 ±0.41)mg/L, t =4.949,P <0.001).Multivariate logistic regression analysis showed adjusted for gender,age and other factors,ser-um cystatin C was independently associated factor of CIMT (OR =2.563,95% CI:1.026 ~6.403,P =0.044). Conclusion Our data suggest that serum cystatin C is significantly associated with carotid atherosclerosis.

Objective: To establish a method for determinating icarin content in Naoling Granule. Methods: Water-soluble ethyl-acetate-extracting polyamide column purification was adopted. The content of icarin was determined by HPLC. C18 column was used, mobile phase was methanol:0.1mol/mL sodium phosphate dibasic (6:5) and detection wavelength was at 270nm. Results: Icarin has a good linearity in the range of 0.24～1.2?g ( r=0.9998). The average recovery was 98.23 %and RSD was 1.2 %(N=5). Conclusion: The method was simple with good reproducibility and can be used for the content determination of icarin in Naoling Granule.

A novel sesquiterpenoid (1) and three known compounds identified as isoaltenuene (2), altenuene (3), and alternariol 4, 10-O-dimethyl ether (4), were isolated in our investigation of the cytotoxic constituents from solid cultures of the endophytic fungus Colletotrichum sp. The structures of these compounds were elucidated through spectroscopic data analysis. All compounds exhibited cytotoxic activity against lung cancer cell line A549, breast cancer cell line MDA-MB-231 and pancreatic cancer cell line PANC-1. Compound 4 could induce the PANC-1 cells inflation or death, but couldn't induce apoptosis at the IC50 of 60.2 microg x mL(-1).

Objective:To study the differences of ventilation function in patients between chronic right heart failure secondary to pulmonary arterial hypertension and chronic whole heart failure secondary to left heart diseases.
Methods:A total of 102 patients with clinical stable chronic heart failure (CHF) were studied. The patients were divided into 2 groups:Whole heart failure (WHF) group, n=41 and Right heart failure (RHF) group, n=61. The ventilation function test at rest and cardiopulmonary exercise test (CPX) were conducted and compared between 2 groups.
Results: The rest ventilation function was similar between 2 groups. For CPX examination, compared with WHF group, RHF group had the lower peak minute ventilation (VE), higher anaerobic threshold and peak minute ventilation/CO2 production (VE/VCO2), higher peak dead space volume/tidal volume (VD/VT), all P<0.05.
Conclusion:At exercise condition, RHF patients had decreased ventilation function and ventilation index due to severe abnormality of ventilation/perfusion (V/Q).

Objective To explore the effect of postoperative rehabilitation nursing for hypertensive intracerebral hemorrhage treated by minimally invasive hematoma evacuation.Methods A total of 100 cases with hypertensive intracerebral hemorrhage treated by minimally invasive hematoma evacuation were randomly divided into two groups.Patients in the control group were given routine nursing,while the experimental group applied rehabilitation care.Hematoma removal,deterioration rate and mortality,related score of disease and rehabilitation before and after intervention,the incidence of related clinical adverse events and family nursing satisfaction were compared.Results The 3 d hematoma clearance rate and overall hematoma clearance rate of the experimental group were significantly higher than that of the control group (P ＜ 0.05).The scores of GSS and AS were significantly higher,and the scores of SSS,FM and ADL were significantly lower than that of the control group(P ＜ 0.05).The incidence of overall adverse events was significantly lower,and nursing satisfaction were significantly higher than that in the control group (P ＜ 0.01).Conclusion Effect of postoperative rehabilitation nursing for patients with hypertensive intracerebral hemorrhage treated by minimally invasive hematoma is significant,and it can improve satisfaction of family members.

Objective To explore the effect of postoperative rehabilitation nursing for hypertensive intracerebral hemorrhage treated by minimally invasive hematoma evacuation.Methods A total of 100 cases with hypertensive intracerebral hemorrhage treated by minimally invasive hematoma evacuation were randomly divided into two groups.Patients in the control group were given routine nursing,while the experimental group applied rehabilitation care.Hematoma removal,deterioration rate and mortality,related score of disease and rehabilitation before and after intervention,the incidence of related clinical adverse events and family nursing satisfaction were compared.Results The 3 d hematoma clearance rate and overall hematoma clearance rate of the experimental group were significantly higher than that of the control group (P ＜ 0.05).The scores of GSS and AS were significantly higher,and the scores of SSS,FM and ADL were significantly lower than that of the control group(P ＜ 0.05).The incidence of overall adverse events was significantly lower,and nursing satisfaction were significantly higher than that in the control group (P ＜ 0.01).Conclusion Effect of postoperative rehabilitation nursing for patients with hypertensive intracerebral hemorrhage treated by minimally invasive hematoma is significant,and it can improve satisfaction of family members.

PURPOSE: Isocryptotanshinone (ICTS) is a natural bioactive product that is isolated from the roots of the widely used medical herb Salvia miltiorrhiza. However, few reports exist on the mechanisms underlying the therapeutic effects of ICTS. Here, we report that ICTS has anticancer activity and describe the mechanism underlying this effect. METHODS: The antiproliferative effect of ICTS was determined using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and clonogenic assays. The effect of ICTS on the cell cycle was measured using flow cytometry. Apoptosis was determined by Hoechst 33342 staining, DNA fragmentation assays, and Western blotting for apoptotic proteins. Finally, the effect of ICTS on mitogen-activated protein kinases (MAPKs) was determined by Western blotting. RESULTS: ICTS significantly inhibited proliferation of MCF-7 and MDA-MB-231 human breast cancer cells, HepG2 human liver cancer cells, and A549 human lung cancer cells in vitro. Among the tested cell lines, MCF-7 cells showed the highest sensitivity to ICTS. ICTS significantly inhibited colony formation by MCF-7 cells. Furthermore, exposure of MCF-7 cells to ICTS induced cell cycle arrest at the G1 phase and decreased mitochondrial membrane potential. Hoechst 33342 staining and Western blot analysis for apoptotic proteins suggested that ICTS induced apoptosis in MCF-7 cells. In addition, ICTS activated MAPK signaling in MCF-7 cells by inducing time- and concentration-dependent phosphorylation of JNK, ERK, and p38 MAPK. CONCLUSION: Our results suggest that ICTS inhibited MCF-7 cell proliferation by inducing apoptosis and activating MAPK signaling pathways.
Apoptosis* ; Blotting, Western ; Breast Neoplasms* ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Line ; DNA Fragmentation ; Flow Cytometry ; G1 Phase ; Hep G2 Cells ; Humans ; Liver Neoplasms ; Lung Neoplasms ; MCF-7 Cells* ; Membrane Potential, Mitochondrial ; Mitogen-Activated Protein Kinases ; p38 Mitogen-Activated Protein Kinases ; Phosphorylation ; Salvia miltiorrhiza

Objective: To analyze the association between different sleep behaviors and overweight and obesity of junior high school students in Yangzhou City, and to provide a basis for policies and interventions related to adolescent health management. Methods: A total of 1 589 students in grades 7-9 from two middle schools in Yangzhou City were selected using the cluster sampling method and were administered with sleep time, bedtime, social jetlag difference, and sleep habits. Results: Totally 64.38% were sleep deprived during the school days, 86.78% went to bed too late, 46.51% had a social jetlag of ≥1 h, and 37.44% took a nap every day(Incluldes holidays and school days). Social jetlag length was statistically different between grades( F =6.97, P < 0.01 ). Girls[(0.95±0.65)h] shown significantly higher social jetlag than the boys[(0.76±0.59)h]( t=6.19, P <0.01). Later bedtime on weekends, later wake up time on weekends and poor sleep behavior were risk factors for overweight and obesity in junior high school students( OR=1.20, 1.14, 1.04, P <0.05). Conclusion Junior high school students had less sleep and later bedtimes with the increase of grade, and weekend bedtimes,wake up times and poor sleep behavior were independently associated with the risk of overweight and obesity in junior high school students. Parents and schools should be instructed to pay attention to their sleep health and carry out adolescent sleep health guidance.