1.The expression of Tregs and CD8+T cells in malignant melanoma and its relationship with prognosis
Yueying SU ; Xiumei AN ; Hua ZHAO ; Feng WEI ; Xinwei ZHANG
Tianjin Medical Journal 2015;(9):1026-1029,1030
Objective To investigate the different distribution of regulatory T cells (Tregs) and CD8+T cells in the local immune microenvironment of mucosal malignant melanoma and cutaneous malignant melanoma, and analyze the relationship between the two indicators and the prognosis of patients. Methods Immunohistochemistry staining was used to assess the ex?pression of Foxp3+Tregs and CD8+T cells in tumor microenvironment of 58 patients with malignant melanoma. The correlation between two factors, clinicopathological characteristics, and prognosis were analyzed. Results There is no correlation be?tween the expression of Foxp3 and CD8. The number of Foxp3+Tregs was significantly higher in mucosa malignant melanoma than that in cutaneous malignant melanoma (t=2.648, P=0.011). The proportion of Foxp3highTregs was significantly higher in pa?tients with tumor diameter≥3 cm, lymph node metastasis and distant metastasis than that in patients with tumor diameter<3 cm, no lymph node metastasis and no distant metastasis (P<0.05). In addition, in patients with ulceration that proportion was significantly higher in CD8high group than that in patients without ulceration (33.3%vs 5.9%, P<0.05). The median progres?sion-free surial (PFS) was 12 months in Foxp3high group, which was significantly longer than that of Foxp3low group (31 months, P<0.05). The median PFS was significantly higher in CD8high group (25 months) than that of CD8low group (12 months, P<0.05). Subgroup analysis showed that the median PFS was 7 months in Foxp3high CD8low group, which was significantly lower than that of Foxp3highCD8high group (25 months) and Foxp3lowCD8low group (18 months, P=0.003). Univariate analysis showed that median PFS was different in patients with different tumor location, different number of Foxp3+Treg, different number of CD8+T cells, and distant metastases. Conclusion The number of Tregs is closely associated with metastasis in patients with malig?nant melanoma. Compared with cutaneous malignant melanoma, our results indicate that the poor prognosis of mucosal malig?nant melanoma may be associated with the infiltration of more Tregs.
2.Vacuole formation and endothelial damage in microvessels after ischemia reperfusion
Yuying LIU ; Xiumei ZHAO ; Fengying LIU ; Niu TIAN
Chinese Journal of Pathophysiology 2001;17(8):771-
AIM:Damaging of Endothelial cells and adhesion of leukocytes and platelets were studied in rat mesentery microvessels after ischemia-reperfusion.METHODS:The model was made by losing blood and reperfusion from carotid artery in rat. Changes in mesentery microvessels were observed by high magnify microscope.RESULTS:Leukocyte and platelet adhesions were found in venules and co-capillaries 1-3 hours after ischemia-reperfusion. Endothelial cells were edema and vascular walls were thickening. Vacuoles formed in intracytoplasm of some vascular endothelium and some bigger endothelial vacuoles prominenced toward the luminal surface. Vacuoles had the shape of circle and the diameter was 10-30 μm. More vacuoles were found in arterioles, even there were several vacuoles in a arteriole. The biggest vacuole almost occupied 2/3 of vascular lumen.CONCLUSION:Edema and vacuole formation in vascular endothelium showed endothelial cells were damaged seriously after ischemia-reperfusion.
3.Actions and prevention of adhesion molecules in complications of diabete mellitus
Furong WANG ; Xiumei ZHANG ; Ling GAO ; Jiajun ZHAO
Chinese Pharmacological Bulletin 1987;0(02):-
Adhesion molecules(AMs) is one kind of glycoproteins expressed on cell surface that can induce interactions between cells or cell and ECM. AMs are indicated to be involved in many chronic complications of diabetes mellitus. Thus, drugs that can decrease levels of AMs are potential new prevention and therapy of diabetic angiopathy.-
4.Effects of salusins on in vivo mesenteric microcirculation in rats
Xiumei ZHAO ; Fengying LIU ; Xiuhua LIU ; Chaoshu TANG
Chinese Journal of Pathophysiology 1986;0(03):-
AIM: To investigate the regulatory effects of salusins on tensility of microvessle in rats. METHODS: Wistar rats were randomly distributed into six experimental groups as follows: (1) normal saline injection-treated control; (2) salusin ?; (3) salusin ?; (4) noradrenaline; (5) noradrenaline+salusin ?; and (6) noradrenaline+Salusin ?. For recording the microcirculation images in the mesentery, the intestinal loop was mounted on the stage of an intravital microscope equipped with a TV camera. Temporal changes in internal diameter of arterioles and venules were measured by computer using the Image-Pro software. RESULTS: Salusin ? (10 -5mol/L) dilated arterioles directly and the dilation up to the peak at 3 min [(25.56?4.30) vs (24.49?4.27)?m, P0.05). Salusin ? has no effects on internal diameter directly and NA-induced contraction of microvessles. CONCLUSION: Salusin ? dilated mesenteric arterioles and inhibited NA-induced contraction of microvessles in rats.
5.Anethole Trithione for Primary Sjogren's Syndrome:Clinical Observation of Curative Effect
Qin WANG ; Fengmei ZHAO ; Chunfang WANG ; Xiumei LIU
China Pharmacy 2001;0(11):-
0.05). CONCLUSION: Anethole trithione has proved efficacy for primary Sjogren's syndrome including xerostomia and xerophthalmia.
6.Inhibition of calcineurin is involved in cardioprotection induced by ischemic postconditioning in rats
Shutong YAO ; Xiuhua LIU ; Xiumei ZHAO ; Fei RONG
Chinese Journal of Pathophysiology 2000;0(12):-
AIM: To demonstrate the mechanisms underlying cardioprotection induced by ischemic postconditioning(I-postC) via studying the alteration of calreticulin(CRT)/calcineurin(CaN) signaling pathway in rat heart subjected to ischemia/reperfusion(I/R).METHODS: The model of myocardial I/R injury in vivo was made by occluding the left anterior descending artery for 45 min followed by 24 h of reperfusion in Wistar rats.Hemodynamics and activity of lactate dehydrogenase(LDH) and creatine kinase-MB(CK-MB) in plasma were measured.Myocardial infarct size was measured by 2,3,5-triphenyltetrazolium chloride(TTC) staining and cardiomyocyte apoptosis was detected using in situ TDT-mediated dUTP nick end labeling(TUNEL).The activity of CaN,the expressions of CaN and CRT in myocardium were detected by enzyme reaction phosphorus measurement and Western blotting analysis,respectively.RESULTS: Cyclosporin A,the inhibitor of CaN,limited significantly myocardial infarct size and cardiomyocyte apoptosis induced by I/R,but had no significant effect on cardiac function.I-postC ameliorated significantly the cardiac dysfunction induced by I/R.Compared with those in I/R group,the myocardial infarct size,the LDH and CK-MB activities in plasma and the cardiomyocyte apoptotic index were significantly reduced in I-postC group.In addition,I/R-induced upregulation of CaN activity,CaN and CRT expression were relieved by I-postC.No significant difference was found between I-postC and ischemic preconditioning groups.I-postC had stronger protective effect on the reperfused heart compared with cyclosporin A.CONCLUSION: The findings indicate that I-postC protects myocardium against I/R injury,at least in part,via inhibiting the CRT/CaN signaling pathway.
7.Effect of WeChat on nutrition instructions to women of advanced maternal age with gestational diabetes mellitus
Xiaoying HUANG ; Qiucheng YANG ; Zhao XU ; Xiumei LIANG
Modern Clinical Nursing 2016;15(4):51-54
Objective To investigate the effect of WeChat on nutrition instructions to women of advanced maternal age with gestational diabetes mellitus (GDM). Methods Eighty women of advanced maternal age with GDM in our hospital during January to December 2014 were divided according to their registration nubmer into two group in eqaul number: those with even number in the observation group and those with odd number in the control group. Those in the control group took part in the health educaiton lectures by the parturient school and those in the observation group were instructed on the WeChat besides education from the school. The two groups were compared in terms of blood glucose control and delivery outcomes. Result The level of blood glucose in the observation group was significantly lower than that of the control group (P<0.05) and the rates for cesarean delivery and vaginal birth were significantly lower and the rate of spontaneous labor was significantly higher than those of the control group (P>0.05). Conclusion Wechat is effective in providing the women of advanced maternal age with nutrition instructions and beneficial for the control of blood glucose level and the decrease of cesarean delivery and vaginal birth rates.
8.Research on work engagement and its influencing factors of clinical nurses
Xue WANG ; Xiumei BU ; Xiaoshuang ZHAO ; Hongmei JIN
Chinese Journal of Practical Nursing 2015;31(19):1467-1470
Objective To know about the current status of the work engagement of the clinical nurses and its influencing factors,to explore the influencing factors of work engagement of clinical nurses so as to provide basis for improving the level of the work engagement of the clinical nurses.Methods The work engagement scale,hardiness scale,role pressure scale,perceived organizational support scale and questionnaire of family care index were used to investigate a total of 630 clinical nurses.The results were analyzed.Results The average score of work engagement,hardiness,role pressure and perceived organizational support were showed respectively as following:(3.28±0.51),(2.60±0.53),(3.00±0.50),(3.43± 1.04) scores.Clinical nurses number of the total point between 7 and 10 of family support accounted for 70.9%(447/630).Hardiness,perceived organizational support,family support and work engagement were in obvious positive correlation,whereas role pressure was in obvious negative correlation with work engagement.Hierarchical regression analysis showed after the variable of demographic was controlled,the hardiness and perceived organizational support respectively had significant positive predictive function on work engagement as respective independent variables.Role pressure had obvious negative predicative function on work engagement.Conclusions Hospital managers should create a good working environment for clinical nurses,measures should be taken from individual and organizational aspects so as to improve the hardiness and perceived organizational support,reduce role pressure and the work positivity of the clinical nurses,so that the nurse more positively devoted to their work.It has important practical significance to improving the quality of their services and stabilizing nursing troop.
9.Study on Pharmacological Actions of Kangleifenghsi Capsules
Shan WAN ; Ling SONG ; Weihong XU ; Aixia ZHAO ; Xiumei YUE ;
Chinese Traditional Patent Medicine 1992;0(08):-
Objective: To study the anti inflammatory, antipyretic and analgetic actions of Kangleifengshi capsules. Methods: The anti inflammatory action was observed by adjuvant arthritis and other inflammatory models. The antipyretic action was surveyed by pyretic rat induced by yeast. The analgetic effect was tested by sprain mice. Results: The experiments proved that the capsule has significant preventing and treating effects on rat adjuvant arthritis, strong inhibition on many kinds of inflammatory models, antipyretic action on pyretic rat induced by yeast and notable angalgetic action on sprain mice induced by acetic acid. Conclusion: Kangleifengshi Capsules has better anti inflammatory, antipyretic and analgetic effects.
10.Clinical effects of Yiqihuoxue decoction on remnant stomach paralysis syndrome after subtotal gastrectomy
Yanfen BAO ; Xiumei ZHOU ; Shuyun BAO ; Wenjie ZHAO
International Journal of Traditional Chinese Medicine 2010;32(5):424-425
Objective To approach effect of Yiqihuoxue decoction on remnant stomach paralysis syndrome after subtotal gastrectomy. Methods A total of 44 patient of remnant stomach paralysis syndrome were randomly recruited into a control group and a treatment group, with 22 patients in each group. The control group was treated with TPN parenteral alimentation. On this basis, the treatment group was treated with Yiqihuoxue decoction and mental intervention. The clinical effects were observed in both groups after the treatment. Results The total effective rate was 54.55% and 100% in the control group and the treatment group respectively, showing statistic difference(P=0.00625 <0.01).Conclusion It was effective to treat remnant stomach paralysis syndrome with Yiqihuoxue decoction, TPN parenteral nutrition and mental intervention.