ObjectiveTo compare therapeutic effects of fluticasone propionate aerosol combined with fluticasone nasal pray and alone fluticasone propionate aerosol for bronchial asthma complcated with allergic rhinitis.MethodsFifty asthmatic children with allergic rhinitis were randomly divided into observation group and control group.Observation group were treated with fluticasone propionate aerosol combined with fluticasone nasal pray,control group were treated with fluticasone propionate aerosol alone.All the patients were observed for 6 months.The lung function parameters( FEV1％ ),the number of acute asthma symptom,the number of days without acute asthma symptom,the number of acute allergic symptoms at one month,three months,six months after the treatment.were observed.ResultsFEV1％of observation group were(107.2 ± 15.3)％,( 115.3 ± 11.9)％,( 131.3 ± 10.9)％,and were significantly improved(98.6 ± 13.6)％,( 102.6 ± 13.6)％,( 126.5 ±9.7)％ 、(t =2.306,3.355,3.714,all P ＜0.05) at one month,three months,six months after the treatment.The number of acute asthma symptom,the number of days without acute asthma symptom,the number of acute allergic symptoms of observation group were(0.13 ±0.11),(150.2 ±14.3),(0.25 ±0.31),and control group were(0.30 ±0.44),(116.5 ±23.1),(0.65 ±0.24),Observation group had significant difference compared with control group(t =2.413,3.435,3.734,all P ＜0.05).ConclusionThere was significant therapeutic effect of fluticasone propionate aerosol combined with fluticasone nasal pray for bronchial asthma complicated with allergic thinitis,and was worthy of clinical application

BACKGROUND:Self-assembling peptide scaffolds have attracted much more attention among three dimensional biomaterials due to its biocompatibility, biodegradation, and tailor-made properties.
OBJECTIVE:To review the structure and design of functionalized self-assembling RADA peptide, and the recent advances in the use of RADA self-assembling peptide for three-dimensional cel culture in cel therapy applications.
METHODS:Total 224 literatures related to self-assembling peptide and tissue engineering from PubMed, Web of Science and CNKI databases were screened out for this review. The keywords were“self-assembly peptide, tissue engineering”in English and Chinese, respectively. Final y 48 of 224 articles about the design, fictionalization, and three-dimensional cel culture of peptide scaffolds were included in this review.
RESULTS AND CONCLUSION:Self-assembling peptide could undergo spontaneous assembly into wel-ordered interwoven nanofibers in water and rapidly form hydrogel, which physical y mimics the architecture of natural extracel ular matrix to ensure a real three-dimensional microenvironment for cel s. In terms of bio-function, this material can be tailor-made with various bioactive short peptide motifs to promote cel adhesion, proliferation, and differentiation.

Objective To analyze the difference of Young's modulus between cirrhotic patients with different etiologies.Methods There were 1 99 patients with decompensated cirrhosis enrolled in this study, all of them had been diagnosed clinically.There were 139 cases of hepatitis B patients with cirrhosis,26 cases of hepatitis C patients with liver cirrhosis,34 cases of patients with alcoholic cirrhosis,and selected 50 healthy volunteers as control group in the same period.All subj ects underwent liver shear wave elastography examination,measured and recorded the Young's modulus,and compared the difference between each groups.Results The Young's modulus of liver in the healthy control group was (4.81±0.9)kPa,which was lowest in all groups.The Young's modulus of hepatitis B cirrhosis,hepatitis C cirrhosis,alcoholic cirrhosis increased successively,which was (16.3±8.9)kPa,(17.8±4.8)kPa,(30.6±12.3)kPa,respectively,and the difference between the groups was statistically significant(F =27.95,P <0.01).Conclusions There were some differences of the Young's modulus of cirrhotic patients with different etiologies by real-time shear wave elastography.

Objective To investigate the efficacy and influence factors of hysteroscopic guiding endometrial resection on dysfunctional uterine bleeding.Methods One hundred and eighty women with dysfunctional uterine bleeding were selected as our subjects in Maternal and Child Health Hospital of Qinhuangdao from Jan.2009 to Jan.2011.One hundred and fifty which meet with operation indications underwent hysteroscopic guiding endometrial resection.Operation periods,blood loss,adverse effects rate were recorded.Serum follicle stimulating hormone (FSH),luteinizing hormone (LH),estradiol (E2) and progesterone (P) hormone levels were detected at preoperative and postoperative.The efficacy was recorded at 6 and 12 months after the procedure.Results The average operation time and amount of bleeding were (31.2 ± 11.0)min and (32.3 ± 10.5) ml,and no blood transfusion performed during the operation.All cases got the succeed operation.There were no significant difference in terms of serum FSH,LH,E2 and P concentrations before and after treatment(P ＞0.05).At 6 months after treatment,improvement rate was 64.0％ (96/150),and effective rate was 32.0％ (48/150).The total effective rate was 96.0％ (144/150).At 12 months after treatment,the improvement rate was 58.0％ (87/150),effective rate was 36.0％ (54/150).The total effective rate was 94.0％(141/150).There was significant different in terms of total effective rate between 6 months and 12 months after treatment(x2 =113.6,P ＞ 0.05).Postoperative complications occurred in 2 cases,including 1 cases of abdominal pain,and 1 cases of dysmenorrhea.Uterine cavity depth,age and disease duration were the main factors affect the clinical efficacy.(OR(95 ％ CI):-1.14 (-1.17 to-1.07),1.60 (1.31 to 2.08),-1.02(-1.14 to-1.08),P ＜ 0.05).Conclusion Treatment of hysteroscopic guiding endometrial resection on dysfunctional uterine bleeding is proved to be efficacy due to the less adverse reaction.

Objective To investigate the relationship between serum CC10 protein and lung injury induced by mechanical ventilation.Methods Forty healthy Wistar rats of both sexes weighing 200-250 g were randomly divided into 5 groups(n=8 each):group Ⅰ control;group Ⅱ mild lung injury[VT=7 ml/kg,duration of mechanical ventilation(t):2 h];group Ⅲ moderate lung injury(VT=7 ml/kg,t=4 h);group Ⅳ severe lung injury(VT=40 ml/kg,t=2 h);group Ⅴ extremely severe lung injury(VT=40 ml/kg,t=4 h).The animals were anesthetized with intraperitoneal 10% chloral hyrdrate 3.5 ml/kg and tracheostomized.Group Ⅰ received no mechanical ventilation.The animals in group Ⅱ-Ⅴ were mechanically ventilated with air(FiO2=21%,RR=40 bpm,I:E=1:2).The animals were sacrificed at the end of mechanical ventilation.The lungs were immediately removed for microscopic examination and determination of W/D lung weight ratio.The left lung was lavaged.The CC10 protein level in broncho-alveolar lavage fluid(BALF)and serum were determined by Western blotting.The Clara cells in the bronchiolar epithelium were examined by immno-histochemistry.Results The level of CC10 protein in BALF was significantly lower in group Ⅳ and Ⅴ while the serum CC10 protein level was significantly higher in group Ⅱ-Ⅴ than in group Ⅰ.The serum CC10 protein level was positively correlated while the CC10 protein level in BALF was negatively correlated with the severity of lung injury and W/D ratio.Conclusion The serum CC10 protein is closely related to the severity of lung injury induced by mechanical ventilation.

Objective:To establish animal model on the basis of the autoimmune etiology for a subset of cases of Tourette syndrome.Methods:Blood samples were drawn from patients with TS(by DSM-IV)and were sent for further enzyme-linked immunosorbent assays(ELISA)to a laboratory.Eight serum samples with the highest concentration of anti-neural antibody were selected for TS model group,and 8 serum sampled with the lowest concentration of anti-neural antibody were selected for the control group.Osmotic mini pump filled with undiluted TS or control serum were microinfused into the rat striatum at a rate of 0.5 μl /h for 72 h.Stereotypic movements were recorded at 1 d,7 d,14 d and 21 d after microinfusion.Several categories of stereotypy including bites(teeth touching the cage,wood chips,vacuous chewing or other objects except the body),taffy pulling(raises of the forepaw to the mouth and face),self-gnawing,licking not associated with grooming,grooming,head shaking,paw shaking,rearing and episodic utterances(EU)were recorded.Results:The anti-neural antibody serum concentration used for TS model was(0.29±0.06) U/L,and that used in control group was (0.10±0.04) U/L.After infusion of TS sera,stereotypic behaviors in rats was increased significantly[(37.2±7.1) vs.(106.3±11.7),P=0.000].Significant difference were observed in stereotypies scores of TS rats compared to control rats after microinfusion[(106.3±11.7) vs.(31.2±6.2),P=0.000].Conclusion:Stereotypic behaviors are increased in rats after intrastriatal microinfusion of Tourette Syndrome sera under noninflammatory conditions.

Objective:To observe the efficacys and side effects of gefitinib(Iressa)and docetaxel(aisu)as the second-line treatment for advanced non-small cell lung cancer(NSCLC)patients.Methods:A total of 98 patients with advanced non-small cell lung cancer who had failed to previous fimt-line chemotherapy were randomly divided into two groups:gefitinib group and docetaxel group.In the gefitinib group,50 patients took Iressa 250 mg once daily until disease progression or intolerable toxicity occurred.In the docetaxel group,58 patients were treated with aisu 75mg/m~2 i.v.in 60 minutes in day 1.The regimen was repeated every 21 days for at least 2 consecutive cycles.They were assessed on the basis of RECIST evaluation standard of therapeutic effect for solid tumor.Results:In the gefitinib group,the response rate was 22.4%(11/49),the clinical benefit rate was 55.1%(27/49),median survival time was 7.1 months and 1-year survival rate was 35.9%.In the docetaxel group,the response rate,clinical benefit rate,median survival time and 1-year survival rate were 18.8%(9/48),50.0%(24/48),6.9months and 31.5%,respectively(P>0.05).The incidences of Ⅰ～Ⅳ degree and Ⅲ～Ⅳ degree rash were 51.0%and 10.2%in the gefitinib group,significantly lower than those in the docetaxel group (P=0.0000 and P=0.0296).The incidence of Ⅰ～Ⅳ degree diarrhea was significantly higher in the gefitinib group than that in the docetaxel group(P<0.01).The incidence of leukopenia was significantly higher in the docetaxel group than that in the gefitinib group(P=0.0000).The incidences of thrombocytopenia and anemia of Ⅰ～Ⅳ degree were also higher in the docetaxel group (P=0.0000 and P=0.0266).The improvement rate of quality of life was higher in the gefitinib group (P<0.05).Conclusion:Gefitinib has a similar anti-tumor effect as docetaxel on advanced NSCLC patients who have failed to previous fimt-line chemotherapy.Gefitnib can achieve higher improvement rate of quality of life in advanced NSCLC patients,with a lower incidence of toxicity.

Objective To explore the application effects of PDCA circulation on behavior management in discharged patients with first cerebral infarction. Methods Patients with first attack of cerebral infarction were enrolled from the department of neurology of the First Hospital of Anhui Medical University from April 2013 to March 2015. According to their admission numbers, patients were divided into the observation group (94 cases) and the control group (80 cases). Besides of the regular personalized secondary prevention, the observation group was also applied for the PDCA cycle for nine months. Stroke Behavior Change Inventory was used to evaluate the changes of their lifestyle and medication compliance one year after discharge for two groups. Results After the observed patients were discharged one year, the changed extent of these five behaviors including quitting smoking, regular monitoring blood pressure and sugar, appropriate exercise, pressure control in the observation group were 0.00 (0.00, 3.00), 1.68 (1.00, 3.00), 1.00 (0.00, 2.00), 1.20 (0.75, 2.00), 1.00 (0.00, 1.00) points, and these in the control group were 0.00 (0.00, 1.0)0, 1.00 (0.00, 2.00), 0.00 (0.00, 1.00), 1.00 (1.00, 2.00), 0.00(0.00, 1.00) points. The observation group in these parameters had significantly higher than the control group (Z=-3.166--2.419, P < 0.05). The changed extent of these behaviors in the medication compliance such as reducing blood glucose, blood pressure, atrial fibrillation, cardiovascular disease, and stroke prevention in the observed group were 0.00 (0.00, 0.00), 0.00 (0.00, 2.00), 0.00 (0.00, 0.00), 0.00 (0.00, 0.00), 3.00 (3.00, 3.00) points, and these in the control group were 0.00 (0.00, 0.00), 0.00 (0.00, 0.79), 0.00 (0.00, 0.00), 0.00 (0.00, 0.80), 0.00 (0.00, 1.50) points. The observation group in these parameters had significantly higher the above parameters than the control group (Z=-8.081--2.595, P<0.05). Conclusions Application of the PDCA circulation in the patients with first attack of cerebral infarction after their discharge can help them correct lifestyle and increase their medical treatment compliance, which is beneficial for secondary prevention of cerebral infarction.

Traditional Chinese medicine(TCM) injection,which plays a unique role in the clinical treatment,is Chinese original dosage form.But in recent years due to its quality problems or unscientifically clinical use,some adverse reactions have reported,even several injections had serious adverse reactions.So how to develop TCM injection becomes one of the topics the public and scholars most concerned about.Therefore,the three "bottleneck" problems of TCM injection on the current development,which are the material basis and TCM theory,clinical efficacy and safety,and also quality control,were analyzed and discussed in the article.Then the countermeasures were proposed and provided ideas for future research and development of TCM injection.

Background and purpose:PTEN mutation has been found in 20%-40% of malignant gliomas.The common mutant epidermal growth factor receptor(EGFR vⅢ)was reported to coexpress in PTEN-deficient EGFR-expressing tumor.PTEN has been shown to interact directly with FAK and reduce its tyrosine phosphorylation levels to inhibit cell invasion.The invasion of glioma cells with EGFRvⅢ expression and PTEN deficiency is increased.This study was to observe whether PTEN inhibits glioma cell invasion even in the presence of strong pro-invasive signals provided by constitutive EGFR activity.Methods:U87?EGFR cells were transfected with pcDNA3.1 constructs encoding PTEN and the cells invasion levels were detected by transwell invasion assay.The expression of FAK was detected by immunoblotting.FAK expression vector was transfected into U87?EGFR-wtPTEN cells and the change of cells invasion was documented.Results:PTEN and PTEN(G129E)could inhibit cell invasion induced by EGFRvⅢ.PTEN and PTEN(G129E)could decrease the FAK phosphorylation at Tyr397.Over expression of FAK in U87?EGFR-PTEN abrogated PTEN-induced down-regulation of the phosphorylation status of FAK and rescued cell invasion.Conclusions:PTEN could inhibit cell invasion induced by EGFRvⅢ by dephosphorylating FAK.