Objective To study the relationship of plasma BNP monitoring and cardiac function classification in elderly patients with ST-segment elevation acute myocardial infarction which intravenous thrombolysis of the early period.Methods 92 patients with acute myocardial infarction who got recanalization therapies by intravenous thrombolysis within 6 hours after myocardial infarction were selected.All the patients were divided into four groups by Killip classification criteria,the BNP level was compared.Comparison of plasma BNP levels in patients with different LVEF,and statistical analysis were conducted.Results With the increase of cardiac function classification,AMI in patients with BNP levels gradually increased (F =56.144,P ＜ 0.05),LVEF reduction in AMI serum BNP levels gradually increased,the difference was statistically significant (F =79.234,P ＜ 0.05).Conclusion The plasma BNP levels of patients with acute myocardial infarction was higher,the heart function was lower.BNP can reflect the heart function,and can be served as an effective marker of clinical diagnosis observation of therapeutic effect,risk stratification and judgement of prognosis in patients with acute myocardial infarction.

Objective To study health education to diabetes patients using club formal, making health education systematized, individualized, consistent and repeatable. Methods Conducted a questionnaire survey on 160 type 2 diabetes patients according to the WHO diabetes diagnostic standard. Results Patients' understanding of diabetes was changed significantly after education ( P ＜ 0. 01) , and patients' compliance to medical workers and to medication was changed significantly too after health education ( P ＜0. 01 or P ＜0. 05). Conclusion Health education using club formal not only improved the patients'understanding of diabetes, but also improved the patients'activeness and make a good image of nurse in the patients, truly accomplished high quality nursing work for patients'good.

ObjectiveTo compare therapeutic effects of fluticasone propionate aerosol combined with fluticasone nasal pray and alone fluticasone propionate aerosol for bronchial asthma complcated with allergic rhinitis.MethodsFifty asthmatic children with allergic rhinitis were randomly divided into observation group and control group.Observation group were treated with fluticasone propionate aerosol combined with fluticasone nasal pray,control group were treated with fluticasone propionate aerosol alone.All the patients were observed for 6 months.The lung function parameters( FEV1％ ),the number of acute asthma symptom,the number of days without acute asthma symptom,the number of acute allergic symptoms at one month,three months,six months after the treatment.were observed.ResultsFEV1％of observation group were(107.2 ± 15.3)％,( 115.3 ± 11.9)％,( 131.3 ± 10.9)％,and were significantly improved(98.6 ± 13.6)％,( 102.6 ± 13.6)％,( 126.5 ±9.7)％ 、(t =2.306,3.355,3.714,all P ＜0.05) at one month,three months,six months after the treatment.The number of acute asthma symptom,the number of days without acute asthma symptom,the number of acute allergic symptoms of observation group were(0.13 ±0.11),(150.2 ±14.3),(0.25 ±0.31),and control group were(0.30 ±0.44),(116.5 ±23.1),(0.65 ±0.24),Observation group had significant difference compared with control group(t =2.413,3.435,3.734,all P ＜0.05).ConclusionThere was significant therapeutic effect of fluticasone propionate aerosol combined with fluticasone nasal pray for bronchial asthma complicated with allergic thinitis,and was worthy of clinical application

Objective To investigate the expression of peroxisome proliferator-activated receptor?(PPAR?) in human gastric carcinoma and the effect of its ligand 15-deoxy-△~(12,14)-Prostagliandxin J_(2)(15d-PGJ_(2)) on the growth of human gastric carcinoma MGC803 cells.Methods PPAR? and survivin mRNA were examined by RT-PCR;PPAR?,survivin,Skp2 and p27 protein were examined in cells by Western blot;proliferation was examined by MTT assay,apoptosis and cell cycle distribution were examined by flow cytometry;PPAR? protein in tissue was examined by immunohistochemistry;morphological change of apoptosis was examined by Hoechst33342 staining.Results The positive rate of PPAR? protein was significantly higher in gastric carcinomas 75.0%(40 cases) than in their paired adjacent mucosa(25.0%)((40 cases))(and)(normal)(mucosa)(20.0%(40cases)),(and)(there)(was)(no) difference between the latterones. The positive rate of PPAR?mRNA and protein were significantly higher in intestinal gastric carcinoma 95.0%(20 cases)than that in diffuse gastric carcinoma 55.0%(20 cases).Proliferation inhibition rate and apoptosis rate of MGC803 cells after_()24h were all significantly higher following 5,10,20,30 and (40 mol/L) 15d-PGJ_(2)treatment than(0 mol/L)(P

Objective To investigate the related risk factors of gastroesophageal reflux disease (GERD).Methods 1 200 patients were chosen,including 99 GERD patients in the observation group,another 99 patients with non-GERD in control group.Questionnaires and esophageal and gastric pressure measurement method were used to analyze the related risk factors of GERD.LESP LESL,GP,the LESP-GP and LESP/GP's were compared between the observation group and the control group.Results In 1 200 patients participating in the survey,99 patients were confirmed GERD,with a ratio of 8.25％.Multivariate logistic regression analysis showed that eating too full,to eat before going to bed,like greasy food,spicy foods,heavy drinking and smoking were important factors affecting the incidence of GERD.2 LESL,LESP/GP and LESP-GP of the observation group were significantly less or lower than those of the control group,GP was significantly higher than that of the control group,the differences were statistically significant (t =12.216,12.515,8.973,3.437,all P ＜0.05).Conclusion Poor eating habits and lifestyle are important risk factors for GERD,the lower esophageal sphincter dysfunction and intragastric pressure increases in GERD incidence occupies an important position,should take positive interventions.

In order to elucidate the relationship between the pathogenesis of hyperthroidism and the genetic as well as environmental factors, clinical investigation and familial survey were performed on 30 cases each familial and non-familial hyper-thyroidism together with their 287 family members. More over, HLA of 52 members in 10 families with familial hyper-thyroidism were analysed. The results indicated that genetic factors were involevd in 27.3% of hyperthyroidism cases, and environmental factors in 83.3% cases that parental symptoms were not so severe as their siblings, and that the positive frequency of HLA-B60 antigen increased significantly (Pc

Objective: To explore the mental health status and related factors of elderly patients. Methods:150 patients aged 60 years above with chronic diseases were measured by SCL-90. Results: 77.33% of the patients reported significant mental symptoms Multiple linear regression showed that financial condition, physical diseases and housing conditions were major factors that affected the patients' mental health. Conclusion: To improve elderly patients' financial condition, housing conditions and physical health may be important for improving their mental health status.

[Objective] To observe clinical cure effect of revised Yijing Decoction treating deficient menopause.[Method] Take revised Yijing Decoction for treatment of 34 cases and comparison.[Result] 18 cases were cured(53%),12 better(35.3%),4 had no effect(11.7%),the total effective rate was 88.3%.[Conclusion] The revised Yijing Decoction can effectively treat deficient menopause.

OBJECTIVE:To explore the stability of levofloxacin hydrochloride and carbazochrome sodium sulfonate in 0.9%So-dium chloride injection,and provide reference for their compatible use in clinic. METHODS:HPLC was performed on the column of Phenomenex Gemini C18 with mobile phase A of acetonitrile and B of 0.01 mol/L Ammonium biphosphate solution(adjusted to pH 3.0 with phosphoric acid)(gradient elution)at a flow rate of 1.0 ml/min,the detection wavelength was 295 nm for levofloxa-cin hydrochloride and 364 nm for carbazochrome sodium sulfonate,temperature was 30 ℃,and the injection volume was 20 μl. The changes of contents,appearance and pH value of the solution in the mixture were investigated. RESULTS:The linear range was 7.03-80.06 μg/mL for levofloxacin hydrochloride(r=0.9995)and 1.70-34.04 μg/mL for carbazochrome sodium sulfonate(r=0.9998);RSDs of precision and reproducibility tests were no more than 2.0%;recoveries were 98.75%-100.63%and 98.00%-100.83%, and RSDs were 0.65% and 0.99%(n=9),respectively. In normal temperature,the contents of levofloxacin hydrochloride and car-bazochrome sodium sulfonate after mixing with 0.9% Sodium chloride injection within 6 h showed no significant decrease,and the appearance and pH value showed no obvious changes. CONCLUSIONS:The mixing of levofloxacin hydrochloride and carbazo-chrome sodium sulfonate with 0.9% Sodium chloride injection in room temperature is stable within 6 h,they can compatibly use synergistically in clinic.

The advancement in medical technologies dramatically increases the recovery rate of various diseases and promotes human health.Human experiments,as we know,are the essential process for the research and upgrading of novel medical technologies,thus serious issues arise as how to protect the legal rights of human subjects.For the convenience of medical research,many doctors enroll their own patients as subjects in their research projects,in which many ethical issues and even disputes are involved.This thesis gives an ethical analysis on the voluntary will of patients′ informed consent to be the subjects in their doctors′ research projects,and comes up with the justifications for and against,limitation principles for application,and protection mechanism for patients′ legal rights.