Response gene to complement-32(RGC-32)as one of important response genes to complement,involves in many biological functions. Recent studies found that many factors could induce the expression of RGC-32 such as membrane attack complex,cortical hormones,growth factors, luteinizing hormone and transforming growth factor-β and so on. The expression products of RGC-32 play a key role in cell proliferation, differentiation, inflammation, tumor metastasis and other processes, thus to participate in the occurrence of many diseases' development processes. However, it has not be clarified in its exact cell biological function, subcellular localization, gene regulation mechanism. This article will make a brief review on gene structure, functions and roles in cell biology as well as the relationship between human diseases for RGC-32.

Objective To detect the serum thrombus precursor protein(TpP)level in the patients with diabetic microangiopathy and to investigate the relationship between the changes of TpP concentrations and the diabetic microangiopathy.Methods 136 ca-ses of diabetic microangiopathy in the endocrinology and neurology departments of our hospital from July to December 2013 were selected as the research subjects and 58 healthy individuals with physical examination were selected as the normal control group(NC group).The serum TpP,Fib,D-dimer concentrations and PT,APTT were measured.The SPSS14.0 software package was adopted to perform the statistical processing.Results (1).Serum TpP concentration in the diabetic microangiopathy group was significantly higher than that in the NC group with statistical difference(P <0.05).There was no statistical differences in Fib,D-dimer,PT and APTT between the two groups(P >0.05).(2).According to whether or not complicating cerebral infarction,the diabetic microan-giopathy group was divided into two subgroups.and diabetic not merged cerebral infarction group.Serum TpP concentrations in the complicating cerebral infarction subgroup was significantly higher than that in the non-complicating cerebral infarction subgroup with statistical difference(P <0.05 ).Conclusion The serum TpP concentration in the diabetic microangiopathy is significantly higher than that in the control group,which indicating that the patient is in a hypercoagulable status the risk of thrombosis.The TpP detection may contribute to early diagnosis and timely treatment of thrombotic diseases for these patients.

Analogy is to construct the theory of traditional Chinese medicine in clinical thinking method,which the proper use of this thinking is helpful to improve the clinical thinking of Chinese medicine,contributes to the development of disease differentiation and treatment ideas.This article tried to analyze the diagnosis and treatment of kidney diseases with Professor Zhu Zongyuan on the process,to explore the analogy on disease differentiation treatment guide.

Objective To investigate the effect of liver function with portal vein occlusion (PVO) in various phases and the following restoration portal vein flow on intestinal mucosal cells. Methods Twenty-four healthy adults white Japanese rabbits were randomized into 1 control group and 2 experimental groups (according to portal vein clamping for 30 min and 45 min). Each experimental group's blood samples were collected from caval vein 1 h before operation, by the end of portal vein oc-cluded, 30 min, 60 rain after relief of portal vein blocking, then with restoration of portal vein flow for 2 h and rabbit guts were continuously cut to sections for HE, TUNEL staining and Bcl-2, Bax protein immunohistochemical staining to observe the injury of intestinal mucosa cell apoptosis and the relation-ship between the expressions of Bcl-2 and Bax. The levels of blood glutamate-pyruvate transaminase (ALT), glutamic oxalacetic transaminase (AST) were measured and compared. Results The levels of ALT, AST in the control group did not significantly change. Compared with control group, group B did not change significantly with PVO 30 rnin in liver enzyme and they were significantly increased after portal vein occlusion relief. The levels of ALT and AST were increased obviously at 45 min with PVO, then raised again. Down-regulation of Bcl-2 expression, up-regulation of Bax expression and the increased index of apoptotic cell were found in each experimental group. Conclusion It may be more safe with PVO for 30 min. But the following restoration portal vein flow will bring about ischemia-reperfusion injury that is mainly apoptosis in the small intestine. The index of apoptosis will be raised with time prolongation of PVO.

Nowadays,in instruction of clinical practice,inaprepriate practice resulting from inappropriate instructional concept is one reason responsible for medical entanglement.In order to make instruction of clinical practice to be more standard,in the meantime coodinate the relation between everu aspect,and let it play the important role in cultivating medical talents, it must adopt the most fundamental intructional conecpt, In other words,the instructional concept of human-basis,quality,law-abiding,creation and wholiness.

OBJECTIVE To compare the air sterilization effect of in operating room used by different air sterilization methods.METHODS Four types sterilization methods were used to operating room air.Then sampling monitoring and evaluation were done.RESULTS The qualification rate of air sterilization were 79.17%,62.79%,79.63%,97.22% in peroxyacetic acid fumigating,simple ultraviolet radiation,peroxyacetic acid fumigating combined with simple ultraviolet radiation,ultraviolet light circulation wind air sterilization machine,respectively.The sterilization effect of ultraviolet light circulation wind was better than the others.CONCLUSIONS Ultraviolet light circulation wind air sterilization machine can be used to air sterilization in non-laminar air flow purify operating room.

[Objective] To discuss the cure effect of acupoints application combined with TCM decoctions and auricular point buried-bean on pernicious vomiting. [Method] Choose 65 such cases from April 2011 to March 2012 as control group and another 65 from Nov. 2011 to Sep. 2013 as treatment group. The control one take oral y TCM decoction combined with auricular point buried-bean, the treatment one added with TCM acupoints applica-tion;observe the result after 1 course. [Result] In treatment group, 46 cases were cured, 18 better, 1 was not cured, the total effective rate 98.5%;in con-trol one, they were respectively 40,11,14 and 78.4%; the comparison of them had difference of statistical meaning.[Conclusion] The acupoint application combined with TCM decoction and auricular point buried-bean has obvious cure effect on pernicious vomiting.

Objective To analyze the utilization of Chinese medicines in outpatients of our hospital for the rational use of Chinese medicines in clinic. Methods Drug consumption data during 2010-2012 were collected from the hospital information system database,and drug daily dosages(DDDs)and consumption sum were statistically analyzed. Results The amount of Chinese medicines was 33.07%-36.29%of all drugs costs in the outpatients from 2010-2012. From DDDs’top 10 Chinese medicines during 2010-2012, medicine for cardiovascular medications and urinary system had a great percentage, and the doctors used Chinese medicines reasonably. Conclusion The Chinese medicines uses in outpatients of our hospital is generally rational.

Aim To investigate the inhibitory effects and mechanism of action of isoliensinine (IL) on the proliferation of porcine coronary arterial smooth muscle cells (CASMCs) induced by phenylephrine (Phen) and its mechanisms of action. Methods MTT assay, immunohistochemical method and Western blotting were adopted. Results IL (0.03-3 μmol·L-1) could inhibit the CASMCs proliferation induced by Phen (0.1 μmol·L-1) in a concentration-dependent manner. IL (0.1 μmol·L-1) antagonized Phen-induced overexpression of PDGF-β and bFGF from 0.545±0.026 and 0.47±0.03 to 0.458±0.019 and 0.376±0.017 (P<0.01, P<0.01). IL (0.1 μmol·L-1) also decreased c-fos, c-myc and hsp70 overexpression induced by Phen from 0.57±0.04, 0.44±0.04 and (173±36)% to 0.46±0.05, 0.372±0.021 and (115±35)% respectively (P<0.01, P<0.01, P<0.01). Conclusion IL exerted antiproliferative effect on CASMCs induced by phenylephrine, and its mechanisms were related to decrease the overexpression of growth factors (PDGF-β, bFGF), protooncogene (c-fos, c-myc) and hsp70.

Objective To compare the specificities of the cell membrane stationary phases(CMSP) with cell membrane chromatography(CMC).Methods Cell chromatographic columns were constructed for both rat aorta tissue cells and cultured rat aorta smooth muscle cells.Then the chromatographic affinities of ten ligands of ?-adrenergic receptor(?-AR) with both said chromatographic columns were investigated.Capacity factors(k'),as a chromatographic parameter,were calculated.Results The correlation analysis showed a positive correlation between the rat aorta tissue CMSP and the cultured rat aorta smooth muscle cell CMSP,with correlation factor of r=0.923,P