To investigate Japanese medical education modality such as the seminar,conference,session and so on,this article discussed the advanced experiences which should be used for reference in our medical education,researched proper ways and means according to our own medical education styles and provided reference to innovate medical education for graduate education and continuing education.

Objective To investigate the effect of apoptosis with portal vein occlusion in various degrees or times and the following restoration portal vein flow on intestinal mucosal cells,to analyze its possible pathogenesis. Methods 40 healthy adults white Japanese rabbits were randomly divided into one controlled group and four experimental groups (according to portal vein half-clamping for 30 min, 45 min and portal vein full-clamping for 30 min, 45 min, divided into B1、C1、B2、C2 groups.).Each experimental group with portal vein occlusion on certain times then with restoration of portal vein flow for 2h. At choiced time rabbit guts were continuously cut to sections for HE、TUNEL staining and Bcl-2、Bax protein immunohistochemical staining,to observe the injury apoptosis in intestinal mucosa and the relationship between the expressions of Bcl-2 and Bax protein. Results Compared with control group A, down-regulation of Bcl-2 expression, up-regulation of Bax expression and the index of apoptotic cell increased were found in each experimental group. there exist significantly differences between each groups for Bcl-2 expression (P

Objective To observe the effect of puerarin on the expression of NF-?B65 and TNF-? in kidney of diabetic rats. Methods SD rats were randomly assigned to 5 groups (10 each):normal control group (group A),diabetic group (group B),low-dose,middle-dose and high-dose treatment groups (group C,D and E). After diabetes model was reproduced,animals in group C,D and E were i.p. injected with puerarin in a dose of 40,80 and 160mg/(kg?d),respectively; animals in group A and B were treated with corresponding normal saline. Fasting blood glucose (FBG),urinary albumin excretory rate (UAER),serum creatinine (Scr) and blood urea nitrogen (BUN) were determined at the 8th weekend after treatment. The histological changes in renal cortex were observed with light microscope and electron microscope. The qualitative and semi-quantitative expressions of NF-?B65 and TNF-? in nephridial tissue were determined by immunohistochemical method. Results FBG,UAER,Scr and BUN were higher in group C,D and E than that in group A (P

Objective To know the current situation and its related risk factors for hypertension in elderly residents in Wendeng City. Methods In August, 2008, with a multi-stage stratified random sampling method, 3 415 residents aged 35 yrs were investigated by questionnaire with the "Health information record" of the national integrated chronic diseases prevention and control techniques, and taken physical examination, blood pressure test and related risk factors for hypertension by single-and multivariate analysis of Logistic regression analysis. Results Of the 3 415 residents aged over 35 yrs, 1 257 suffered from hypertension, the prevalence rate was 39.87%, and the standardized prevalence rate was 37.68%, and prevalence rate of hypertension increased with age (?2=285.54, P

Objective To evaluate the relationships between the unstable coronary lesions and the levels of serum C-reactive protein(CRP) and urico-acid (UA).Methods 205 patients with primary diagnoses as coronary heart disease were examined by selective coronary angiography,and in the mean time ,the serum CRP and UA levels were measured.These patients were grouped the control,the unstable coronary lesion group and stable coronary lesion group according to the extent of coronary artery atherosclerosis stenosis.All data were undergone statistical analysis.Results The serum CRP level in unstable coronary lesion group was higher than that in stable coronary lesion group(P0 05).Conclusions The serum CRP level is significantly positive relation with the unstable coronary lesions.The serum UA level is not association with the unstable coronary lesions.

Objective To investigate the clinical features of Mekel diverticulitis,and to improve the cognition rate and diagnosis rate of the disease.Methods The clinical data of 7 patients with Mekel diverticulitis misdiagnosed as acute appendicitis were retrospectively analyzed,and the related literature was reviewed.Results 7 cases of Mekel diverticulitis were misdiagnosed as acute appendicitis before surgery,all corrected diagnosis and operation.One case of mild incision infection after surgery,the remaining 6 cases did not appear complications.After treatment were cured after 7-12d discharged.Conclusion Mekel diverticulitis is lack of specific clinical manifestations and effective auxiliary examination method,easily misdiagnosed as acute appendicitis,when the clinical manifestations of appendicitis is not typical,need to further analysis,identification;when the surgery found appendix lesions are not coincide with clinical practice,the distal ileum should be routinely probed to determine the presence of Meckel diverticulitis.

OBJECTIVE To compare the air sterilization effect of in operating room used by different air sterilization methods.METHODS Four types sterilization methods were used to operating room air.Then sampling monitoring and evaluation were done.RESULTS The qualification rate of air sterilization were 79.17%,62.79%,79.63%,97.22% in peroxyacetic acid fumigating,simple ultraviolet radiation,peroxyacetic acid fumigating combined with simple ultraviolet radiation,ultraviolet light circulation wind air sterilization machine,respectively.The sterilization effect of ultraviolet light circulation wind was better than the others.CONCLUSIONS Ultraviolet light circulation wind air sterilization machine can be used to air sterilization in non-laminar air flow purify operating room.

Objective To investigate the clinical efficacy and safety of intravenous immunoglobulin and interferon in the treatment of hand foot and mouth disease complicated with viral encephalitis .Methods 80 cases of hand foot and mouth disease complicated with viral encephalitis were randomly divided into the three groups according to the random number table .27 cases in the control group were given comprehensive symptomatic treatment ,27 cases in the study group 1 were given gamma globulin ,and 26 cases in the study group 2 were given interferon .The clinical efficacy,improvement of disease ,incidence of adverse reactions of the three groups were compared .Results Defer-vescence time ,seizure control time ,time of skin rash subsided ,time of psychiatric symptoms relieved and the average hospitalization time in the study 1 group were (3.65 ±0.28)d,(4.04 ±0.33)d,(3.86 ±0.27)d,(5.83 ±0.36)d and (7.53 ±0.83)d,which were significantly less than those in the control group (t=8.43,8.58,9.15,9.80,8.96, all P<0.05).Those in the study 2 group were (3.92 ±0.29)d,(4.21 ±0.32)d,(4.27 ±0.30)d,(6.32 ±0.43)d and (8.10 ±0.72)d,which were significantly less than the control group (t=7.99,8.17,8.54,9.18,8.55,all P<0.05);however,there were no significant differences between the study 1 group and the study 2 group(t=1.12, 2.04,1.67,1.38,2.21,all P>0.05).The incidence rate of adverse reaction among the three groups showed no sta-tistically significant difference (χ2 =2.17,P>0.05).Conclusion Intravenous immunoglobulin and interferon have significant effect in the treatment of hand foot mouth disease complicated with viral encephalitis , which can quickly improve symptoms ,shorten treatment time and have high safety and good clinical application value .

Objective: To investigate the expression of EMS1 in laryngeal carcinoma and its clinical signifi-cance. Method:The expression of EMS1 protein was measured in 40 samples of, 40 samples of para carcinoma tis-sues (which were near to cutting margin of laryngeal carcinoma tissuse over 0. 5 cm) ,and 20 samples of normal la-ryngeal mucosa as controls by Flow Cytometere( Epics-XL Ⅱ ). Results:The quantity and percentage of EMS1 pro-tein expression in laryngeal carcinoma tissues was significantly higher than those in para carcinoma and in normal laryngeal mucosa tissues respectively(P<0. 05). There was no significant expression difference between the para carcinoma tissues and normal laryngeal mucosa tissues. There were positive correlation between the expressions of EMS1 protein and metastasis, pathological grade and clinical stage in laryngeal carcinoma. But there were not rela-tionship with patients' clinical classification, tumor size, smoking history, age and sex. Conclusion: The high ex-pression of EMS1 may contribute to the carcinogenesis and development of laryngeal carcinoma. The expression of EMS1 protein is an important index of judging differentiation, infiltration, metastasis and staging of laryngeal car-cinoma.

Due to the characteristics and environmental factors, electrocardiogram (ECG) signals are usually interfered by noises in the course of signal acquisition, so it is crucial for ECG intelligent analysis to eliminate noises in ECG signals. On the basis of wavelet transform, threshold parameters were improved and a more appropriate threshold expression was proposed. The discrete wavelet coefficients were processed using the improved threshold parameters, the accurate wavelet coefficients without noises were gained through inverse discrete wavelet transform, and then more original signal coefficients could be preserved. MIT-BIH arrythmia database was used to validate the method. Simulation results showed that the improved method could achieve better denoising effect than the traditional ones.
Algorithms ; Arrhythmias, Cardiac ; Databases, Factual ; Electrocardiography ; Humans ; Signal Processing, Computer-Assisted ; Wavelet Analysis