To investigate and analyze the distribution characteristics and genetic variation of mitochondrial encephalomyopathy (ME) in children in Hainan province.

Bacillus is a class of spore-producing Gram-positive bacteria that produce a variety of antimicrobial substances with different structures and functions. The application of the antimicrobial substances produced by Bacillus can effectively inhibit the activity of harmful bacteria and fungi and promote the sustainable development of green agriculture. The antimicrobial substances produced by Bacillus mainly include proteins, lipopeptides, polyketones, and polypeptides. This paper reviews the synthesis gene clusters, synthesis pathways, structures, and mechanisms of various antimicrobial substances produced by Bacillus and discusses the challenges in the industrial application of these antimicrobial substances. Furthermore, this paper clarifies the future research and development focuses and prospects the application prospects, and provides comprehensive theoretical support for the in-depth research and wide application of the antimicrobial substances produced by Bacillus.
Bacillus/genetics* ; Anti-Infective Agents/metabolism* ; Bacterial Proteins/genetics* ; Antimicrobial Peptides/biosynthesis* ; Lipopeptides/biosynthesis*

Objective:To analyze immune reconstitution and influencing factors in HIV infected men who have sex with men (MSM) with access to antiviral therapy (ART) in Guangxi Zhuang Autonomous Region (Guangxi) during 2005-2021.Methods:The data were collected from Chinese Disease Prevention and Control Information System. The study subjects were HIV infected MSM with access to the initial ART for ≥24 weeks in Guangxi from 2005 to 2021 and HIV RNA lower than the detection limit within 24 months. The proportion of infected MSM who had immune reconstitution after ART was calculated. Cox proportional hazard regression model was used to analyze the influencing factors of immune reconstitution. Software SPSS 24.0 was used for statistical analysis.Results:A total of 3 200 HIV infected MSM were enrolled, in whom 15.56 % (498/3 200) had no immune reconstitution, 14.78% (473/3 200) had moderate immune reconstitution, and the rate of complete immune reconstitution was 69.66% (2 229/3 200). The M ( Q1, Q3) of ART time for immune reconstitution was 12 (5, 27) months. Multivariate Cox proportional risk regression model analysis results showed that compared with those with initial ART at age ≥30 years, WHO clinical stage Ⅲ/Ⅳ illness, baseline BMI <18.50 kg/m 2 and baseline CD4 +T lymphocyte (CD4) counts <200 cells/μl, HIV infected MSM with initial ART at age <30 years, WHO clinical stageⅠ/Ⅱ illness, baseline BMI≥24.00 kg/m 2 and baseline CD4 counts ≥200 cells/μl were more likely to have complete immune reconstitution. Conclusions:In the HIV infected MSM in Guangxi, failures to achieve moderate and complete immune reconstitution were observed. Surveillance and ART regimen should be improved for key populations, such as those with older age and low baseline CD4 counts.

Objective:To compare and analyze the clinical characteristics and long-term prognosis of patients with deep infiltrating endometriosis (DIE) and non deep infiltrating endometriosis.Methods:A retrospective analysis was conducted on the data of 178 patients with endometriosis who underwent laparoscopic surgery at the General Hospital of Northern Theater Command from January 2016 to January 2018. Among them, 95 patients with DIE were treated as the observation group, and 83 patients with non deep infiltration were treated as the control group. Both groups were followed up for at least 5 years after surgery. Perform statistical analysis on the general clinical data, intraoperative and postoperative follow-up data of the two groups.Results:Compared with the control group, the observation group had significantly older age [(33.8±5.5)years vs (32.0±5.2)years], moderate to severe dysmenorrhea [72.6%(69/95) vs 55.4%(46/83)], chronic pelvic pain [24.2%(23/95) vs 8.4%(7/83)], higher proportion of elevated blood carbohydrate antigen 125 (CA125) [80.0%(76/95) vs 65.1%(54/83)], and higher Visual Analogue Scale (VAS) [(5.4±1.2)points vs (4.3±0.9)points]; At the same time, the surgery time was longer [(75.1±20.1)min vs (56.0±18.9)min], the patient′s intraoperative bleeding was also greater [(79.2±23.0)ml vs (57.8±16.3)min], bilateral cysts [54.7%(52/95) vs 34.9%(29/83)], severe pelvic adhesions [90.5%(86/95) vs 53.0%(44/83)], and the proportion of concurrent adenomyosis [41.1%(39/95) vs 22.9%(19/83)] was higher, with higher Revised American Fertility Society (rAFS) scores [(61.8±22.1) vs (39.4±19.1)]. The proportion of rAFS stage Ⅳ [71.6%(68/95) vs 43.4%(36/83)] was higher, and the proportion of postoperative medication was higher [98.9%(94/95) vs 92.8%(7/83)]. The above indicators showed statistically significant differences between the two groups (all P<0.05 or P<0.01). There was no statistically significant difference in the recurrence rate [21.1%(20/95) vs 15.7%(13/83)] and live birth rate [100%(35/35) vs 92.1%(35/38)] between the observation group and the control group (all P>0.05). Conclusions:Compared to non DIE patients, DIE patients have more severe pain symptoms and pelvic adhesions, higher rAFS scores, and a higher proportion of stage Ⅳ. However, there was no significant difference in postoperative recurrence rate, and there was no significant impact on fertility outcomes.

From the perspective of human development history， epidemic diseases have been accompanied by human life and reproduction， causing serious threats to human health and life safety. Epidemic diseases have a rapid onset and are highly infectious， once they occur， can quickly spread to the people around them， causing many people to be infected in a short time. Pulmonary system epidemic disease is the kind of disease with the highest incidence， prevalence， and infectivity among epidemic diseases， which will also have an impact on the economic and social development and even the historical process. Overview of ancient Chinese literature， the wisdom of ancient doctors has certain reference value for possible related work today or in the future. Starting from sorting out the classical theories recorded in the ancient books of traditional Chinese medicine， this paper dug deeply into them and re⁃understood them， with a view to providing a reference for clinical diagnosis and treatment， and public health services.

Objective:To describe the changes of post-traumatic growth and rumination in coronary heart disease patients at different time points after PCI, and to analyze the correlation between them in order to provide evidence for personalized intervention.Methods:A cross-sectional study was conducted, a total of 234 patients with coronary heart disease who underwent PCI for the first time in the Department of Cardiology, the First Affiliated Hospital of China Medical University from December 2021 to October 2022 were studied by convenience sampling. The general data questionnaire, the Chinese version of the Event-Related Rumination Inventory and the simplified Chinese version of the Posttraumatic Growth Inventory were used to survey before discharge (T 0), first month after surgery (T 1), and three months after surgery (T 2). Results:The total scores of post-traumatic growth at the time point of T 0, T 1 and T 2 were 53.08 ± 13.47, 54.77 ± 11.20 and 59.32 ± 7.95, the difference was statistically significant ( F = 78.45, P<0.01). The scores of instrusive rumination at the time point of T 0, T 1 and T 2 were 14.92 ± 6.07, 15.78 ± 4.64 and 12.31 ± 3.38, the difference was statistically significant ( F = 46.58, P<0.01). The scores of deliberate rumination were 14.63 ± 4.74, 15.52 ± 3.93 and 17.86 ± 3.70, the difference was statistically significant ( F = 76.20, P<0.01). Instrusive rumination at T 0, T 1 and deliberate rumination at each point were positively correlated with the total score of post-traumatic growth ( r values were 0.229 to 0.414, all P<0.01). Instrusive rumination at T 2 was negatively correlated with posttraumatic growth scores ( r = -0.351, P<0.01). The deliberate rumination at T 2 time point was positively correlated with the total score of post-traumatic growth ( r = 0.403, P<0.01). Conclusions:The level of posttraumatic growth in patients with coronary heart disease after PCI needs to be improved, and targeted intervention should be planned early to change patients′pessimistic cognition caused by the disease, promote the development of positive thinking, then promote their posttraumatic growth.

Objective: To explore the current status related to behavioral problems among children and adolescents aged 12-16 years old in Guizhou Province, China, and to provide a reference for timely prevention and intervention measures. Methods: To capture different economic development levels, a multistage stratified cluster sampling method was used. A total of 3 217 children and adolescents aged 12-16 years old from six middle schools and six primary schools in Guiyang, Duyun, Kaili City from Guizhou Province participated in this study. The Achenbach Child Behavior Checklist (CBCL) and a self designed questionnaire were administered between August and December 2021. Results: The overall detection rate of behavioral problems among the students was 16.60% , and the rate was 14.41% and 18.62% among male and female students, respectively. The results of the Logistic regression analysis showed that good peer relationships ( OR=0.25, 0.16, P <0.05) were associated with a lower risk of behavioral problems in children and adolescents. However, the following factors were associated with an elevated risk of behavioral problems: nonparents as the main caregivers( OR = 1.44 ); children s daily screen time was 1-2 h ( OR =1.47) or more than 2 h( OR =2.75); children who had no sports hobbies( OR = 1.36); father parenting for doting or neglect( OR =1.85, 2.01); maternal parenting for doting( OR = 2.12 ); father had bad life habits( OR =1.69); father and mother were occasionally sick( OR =1.30, 1.38)( P <0.05). Conclusion Behavioral problems among children and adolescents in Guizhou Province is related to children s unhealthy living habits and various factors in the family environment. It is suggested that families, schools and relevant functional departments give full play to their own advantages and take comprehensive measures to prevent the occurrence of behavioral problems among children and adolescents.

Objective:To analyze the clinical features and long-term prognosis of ovarian endometrioma (OMA) with deep infiltrating endometriosis (DIE).Methods:A retrospective analysis was conducted on the clinical data of 178 OMA patients admitted to the Northern Theater General Hospital from January 2014 to January 2017. All patients received laparoscopic ovarian cyst removal. There were 83 patients with OMA (control group) and 95 patients with OMA combined with DIE (observation group) diagnosed by postoperative pathology.The general clinical data of patients were collected, including age, number of pregnancies, body mass index (BMI), duration and severity of dysmenorrhea, levels of carbohydrate antigen 125 (CA125), type of infertility, and other pain symptoms.The surgical related indicators of patients were collected, including the operation time, intraoperative bleeding, cyst diameter and location, whether combined with adenomyosis, severe pelvic adhesion, and the grading and staging of the American Fertility Society's revised staging standard for Endometriosis (rAFS). The postoperative follow-up indicators of patients were collected, including postoperative medication, medication regimen, medication time, pregnancy outcome, recurrence status, and type of recurrence (pain recurrence, cyst recurrence). The measurement data was represented by xˉ± s, and independent sample t-test was used for inter group comparison.The counting data was represented by example(%), and χ 2 test was used for inter group comparison. Results:The age, VAS score, moderate and severe dysmenorrhea, chronic pelvic pain, and CA125 elevation in the observation group were higher than those in the control group [(33.8±5.5) years vs (32.0±5.2) years, (5.4±1.2) points vs (4.3±0.9) points, 72.6% (69/95) vs 55.4% (46/83),24.2%(23/95) vs 8.4%(7/83), 80.0% (76/95) vs 65.1% (54/83)], the differences between the two groups were statistically significant ( t=2.23、 P=0.031, t=6.83、 P<0.001, χ 2=5.74、 P=0.017, χ 2=7.87、 P=0.005, χ 2=5.02、 P=0.025). The operation time in the observation group was longer than that in the control group [(75.1±20.1) min vs (56.0±18.9) min], the intraoperative bleeding was more than that in the control group [(79.2±23.0) mL vs (57.8±16.3) mL], and the proportion of bilateral cysts, severe pelvic adhesion, combined adenomyosis and r-AFS stage Ⅳ and rAFS score in the observation group were higher than those in the control group [54.7%(52/95) vs 34.9%(29/83),90.5% (86/95) vs 53.0% (44/83), 41.1% (39/95) vs 22.9% (19/83), 71.6% (68/95) vs 43.4 (36/83), (61.8±22.1) points vs (39.4±19.1) points], the differences between the two groups were statistically significant ( t=6.50、 P<0.001, t=7.06、 P<0.001, χ 2=8.27、 P=0.016, χ 2=31.65、 P<0.001, χ 2=6.65、 P=0.010, χ 2=14.69、 P=0.002, t=7.18、 P<0.001). The proportion of postoperative medication in the observation group was higher than that in the control group [98.9% (94/95) vs 92.8% (77/83)], the difference between the two groups was statistically significant (χ 2=4.47、 P=0.034). In the observation group 35 cases of postoperative pregnancy were all alive (100.0%), and in the control group 38 cases of postoperative pregnancy 35 cases were alive (92.1%), there were 3 cases of spontaneous abortion.There was no statistically significant difference in pregnancy outcomes between the two groups (χ 2=2.88, P=0.090). Conclusions:The OMA with DIE patients have more severe pain symptoms than those without DIE, severe pelvic adhesion is more during operation, r-AFS score is higher and more in stage Ⅳ, but there are no significant differences in the total recurrence rate and fertility outcome after operation

Objective:To explore the effect of clinical conventional fractionated dose radiation on the expression levels of immunogenic cell death (ICD) related proteins in patients with nasopharyngeal carcinoma (NPC).Methods:A total of 38 newly-treated NPC patients admitted to the Affiliated Cancer Hospital of Guizhou Medical University from November 2020 to December 2021 were enrolled, all of whom received induction chemotherapy and concurrent chemoradiotherapy, and another 20 healthy volunteers were selected as controls for a prospective study. The contents of ICD related proteins, namely calreticulin (CRT), high mobility group box 1 protein (HMGB-1) and heat shock protein 70 (HSP70) and the proportion of dendritic cell (DC) in the peripheral blood of patients were detected before treatment, after induction chemotherapy and after concurrent chemoradiotherapy, respectively. The correlation between the above indicators, general clinical data and short-term efficacy was analyzed by statistical methods such as t-test and analysis of variance (ANOVA). Results:The levels of HSP70 and HMGB-1 in peripheral blood of NPC patients before treatment were higher than those of healthy controls (both P<0.05). After concurrent chemoradiotherapy, the content of CRT was significantly higher than that before treatment ( P<0.05), whereas the difference before and after induction chemotherapy and the difference before and after concurrent chemoradiotherapy were not significantly correlated with the short-term efficacy of NPC patients. HSP70 level was significantly decreased after concurrent chemoradiotherapy ( P<0.001). There were no significant differences in the content of HMGB-1 after induction chemotherapy and concurrent chemoradiotherapy (both P>0.05). Conclusion:NPC patients receiving TPF regimen (docetaxel+cisplatin+fluorouracil) for induction chemotherapy and sequential cisplatin concurrent chemotherapy may induce ICD in NPC cells, and CRT has potential value in reflecting the clinical efficacy of NPC.

Objective:To explore the effects of Onodera′s prognostic nutritional index (PNI) on the prognosis of locally advanced oropharyngeal squamous cell carcinoma (LA-OPSCC) after induction chemotherapy followed by sequential chemoradiotherapy.Methods:A retrospective analysis was conducted on the clinical data of 52 LA-OPSCC patients receiving induction chemotherapy followed by sequential chemoradiotherapy in The Affiliated Cancer Hospital of Guizhou Medical University during 2014-2018. The PNI values of all the patients at different treatment phases were statistically analyzed, and the ROC curve was employed to determine the optimal critical value of PNI. The patients in this study were divided into a well-nourished group ( n = 27) and a poorly-nourished group ( n = 25). The Kaplan-Meier method was used for survival analysis. The Cox proportional hazards model was utilized to analyze the relationships between different nutritional status and prognosis. Clinical features and adverse reactions were compared between the two groups. Results:The PNI values decreased significantly after radiotherapy, with an optimal critical value of 42.4. The 5-year overall survival (OS) and progression-free survival (PFS) of the well-nourished group (PNI ≥ 42.4) were 62.6% and 60.9%, respectively, which were significantly higher than those (30.1% and 29.7%) of the poorly-nourished group (PNI < 42.4, χ2 = 11.12, 5.74, P < 0.05). The multivariate analysis showed that PNI was an independent prognostic factor for the OS after radiotherapy ( HR = 2.752, 95% CI: 1.095-6.917, P = 0.031). The LA-OPSCC patients aged over 60 years or those who did not respond to induction chemotherapy accounted for a higher proportion of malnutrition after chemoradiotherapy ( χ2 = 4.89, 5.05, P < 0.05). Conclusions:PNI after radiotherapy can be used as a prognostic factor in the evaluation of LA-OPSCC patients receiving induction chemotherapy followed by sequential chemoradiotherapy. The LA-OPSCC patients aged over 60 years or those who do not respond to induction chemotherapy should receive more nutritional support during the chemoradiotherapy.