Objective To explore the clinical significance in combined detection of C-reactive protein and the cervical secretion smear in chorioamnionitis with preterm premature rupture of membranes (PPROM).Methods Eighty patients with PPROM (PPROM group) were selected and divided into chorioamnionitis group (n =55) and without chorioamnionitis(n =25) according to the diagnosis.The levels of C-reactive protein and cervical smear were analysis,including sensitivity,specificity,positive predictive value and negative predictive value.Sixty cases of preterm no premature rupture of membranes pregnant women with the same gestational age were chosed as the control group.Results The positive rate of CRP and cervical secretions smear in PPROM group were higher than those of control group,and the differences were statistically significant (x2 =50.24,54.81 ;P ＜ 0.05).The sensitivity,specificity,positive predictive value and negative predictive value by the CRP diagnosis of chorioamnionitis in patients with PPROM were 58.18％,63.64％,36.00％,32.00％ respectively.Those indices of cervical secretions smear diagnosis were 66.67％,67.31％,29.03％,28.57％ respectively.The sensitivity,specificity,positive predictive value regarding of CRP and cervical secretions smear joint inspection diagnosis of chorioamnionitis were 69.09％,88.00％,92.68％,56.41％ respectively.Conclusion The inspection of CRP combined with cervical secretion smear can improve specificity and positive predictive value of chorioamnionitis diagnosis with PPROM pregnant women.

Endogenous formaldehyde is generated in the human body.When the system of endogenous formaldehyde generation with scavengation is damaged,excess accumulation of endogenous formaldehyde induces vascular endothelial injury,atherosclerosis,myocardial damage and neurodegenerative diseases.Studies show that endogenous formaldehyde is one of the key factors during the process of cardiovascular and cerebrovascular diseases.Moreover,polyphenols are used as capture agents of endogenous formaldehyde to prevent and treat cardiovascular and cerebrovascular diseases.

Objective To estimate the diagnostic value of interstitial infiltration pattern for early morphea.Methods Twenty-five cases of early morphea pathologically characterized by interstitial infiltration of inflammatory cells were collected from 2010 to 2012.The clinicopathological features of these cases were retrospectively analyzed.Results The average clinical course was 7.5 months.The primary manifestation was edematous dark erythematous plaques,and interstitial or mixed infiltrate of inflammatory cells was the characteristic histopathological presentation.After anti-inflammatory treatment,lesions markedly improved or disappeared in 70％ of these patients.Conclusions Interstitial infiltration of inflammatory cells is a rare histologic pattern in early morphea.To learn and recognize this pattern may be beneficial to the diagnosis and treatment of early morphea.

Compound Danshen aerosol remarkably increased hypoxia tolerance after isoproterenol injection and prolonged survival time in mice. The electrocardiogram of rats and rabbits with pituitrin-induced ischemia was significantly improved. The aerosol also decreased the elevation of lactic dehydrogenase in rats with experimental myocardial infarction.

Objective To identify the gene locus and the mutation of DSRAD (double-stranded RNA adenosine deaminase) in a Chinese dyschromatosis symmetrica hereditaria(DSH) family. Methods After confirming the diagnosis of the DSH proband, the genomic DNA was extracted from the whole blood samples of every members of the pedigree. The DSRAD gene intervals were localized by linkage analysis and haplotype reconstruction. The mutation of DSRAD was detected by direct sequencing. Results The candidate gene was localized at the 1q region, consistent with the reported region. The direct sequencing results showed that there was a CAA→TAA transition at exon 2 of DSRAD in all affected family members, which consequently led to a nonsense mutation of Gln517Ter. Conclusion A nonsense mutation is found in the Chinese DSH family.

Objectives To synthesize human telomerase reverse transcriptase (hTERT)- and human tolemerase RNA (hTR)- small interfering RNA (siRNA) and investigate their effects on telomerase activity in the cutaneous T-cell lymphoma (CTCL) cell line Hut78. Methods Two types of hTERT- and hTR- siRNAs were synthesized with T7 RNA polymerase via in vitro transcription, then either mixed with Hut78 cell lysates directly or transfected into Hut78 cells by calcium phosphate co-precipitation. Telomerase activity was tested by telomeric repeat amplification and polyacrylamide gel electrophoresis. Results With T7 RNA polymerase, hTERT- and hTR- siRNAs were synthesized efficiently with a concentration of 22.4?g siRNA per 40?L siRNA reaction mix. Telomerase activity was suppressed significantly by either of the siRNAs. The inhibition rate was 87% in the cell lysate group treated with siRNA directly, and 75% in the cell group Iransfected with siRNA. Conclusions The in vitro transcription of siRNA with T7 RNA polymerase is technically simple, costeffective, and can produce siRNA in an efficient way. hTERT- and hTR-siRNA can down-regulate telomerase activity significantly in Hut78 cells.

Objective To investigate the effects of chemokine CCL18 on the proliferation and invasion of a human melanoma cell line A375. Methods Human peripheral blood monocytes were isolated from healthy volunteers, cultured in vitro and divided into two groups to be induced by IL-4 for 48 hours or remain untreated. A375 cells were classified into 3 groups to be cultured with IL-4-induced monocytes, untreated monocytes or CCL18 of 200 g/L for various durations. A375 cells receiving no treatment served as the control.MTT assay was performed to detect the proliferation of A375 cells, chemotaxis test and Matrigel-transwell assay to evaluate the chemotaxis and invasion ability of A375 cells, and chicken chorioalllantoic memebrane (CAM)was used to detect the effect of CCL18 on tumor angiogenesis. Results The proliferation of A375 cells was statistically accelerated by IL-4-induced monocytes and untreated monocytes, but unaffected by CCL18.Matrigel-transwell assay revealed that IL-4-induced monocytes and CCL18 promoted the chemotaxis and invasion ability of A375 cells (all P＜0.05). Tumor angiogenesis was also increased by IL-4-induced monocytes and CCL18 (both P ＜ 0.05). Conclusion The chemokine CCL18 can promote the invasion ability of A375 cells and tumor angiogenesis.

Objective To investigate the effects of HINT1 on the growth of and apoptosis in malignant melanoma in nude mouse models established by subcutaneous xenotransplantation of human melanoma A375 cells. Methods Three groups of nude mice were subcutaneously inoculated with A375 cells transfected with pcDNA 3.1/myc-His (-) A-HINT1 (HINT1-A375), A375 cells transfected with pcDNA 3.1/myc-His (-) A empty vector (neo-A375), and untransfected A375 cells, respectively. Then, the growth of transplanted tumors was observed and tumor formation rate was calculated. Thirty-three days after the inoculation, mice were killed and tumor tissue was obtained followed by the examination of tumor weight and volume. Histopathology was performed to observe the morphological features of tumor cells and in situ end labeling technique (TUNEL)was carried out to assess the apoptosis in transplanted tumor cells. Results The tumor formation rate was consistently 100% in the three groups. The transplanted tumor in HINT1 group grew more slowly than that in the other two groups, and significant difference was observed as early as day 18 (P ＜ 0.01 ). Lower tumor weight and volume were noted in the HINT1 group compared with the neo group and A375 cell group (0.04 ±0.00 g vs. 0.23 ± 0.00 g and 0.29 ± 0.03 g, 0.06 ± 0.04 cm3 vs. 0.34 ± 0.15 cm3 and 0.43 ± 0.19 cm3 respectively, all P ＜ 0.01 ). Histopathology revealed smaller tumor nests, slight atypia of tumor cells with no obvious pathologic mitoses or necrosis in HINT1 group in comparison with the other two groups. Immunohistochemistry and TUNEL revealed that the percentage of apoptotic cells in HINT1 group was statistically higher than that in the neo group and A375 cell group (12.87% ± 1.18% vs. 3.22% ± 0.49% and 3.00% ± 0.53%, both P ＜0.01 ). Conclusion High expression of HINTl could inhibit the growth of and promote the apoptosis in malignant melanoma in nude mice subcutaneous xenotransplantation models, suggesting that HINT1 gene might be responsible for tumor suppression in human malignant melanoma.

ObjectiveTo understand the clinical and histopathologic diagnostic criteria for aneurysmal fibrous histiocytoma(AFH).MethodsThe clinical and histopathological features of 5 patients with AFH were retrospectively reviewed.ResultsThere were 3 males and 2 females in these patients.All the tumors clinically manifested as dark erythematous or brown nodules.Three cases had a recent history of rapid growth.The lesions were located on the limbs(n =3),or chest and lower mandible(n =2).Histopathological examination of skin biopsies showed typical features of dermatofibroma,accompanied by many irregular cleftlikeorcavernousblood-filledspaceswithnumeroushemosiderinpigmentsinallofthesecases.Immunohistochemically,the tumor cells were immunoreactive to vimentin and CD68 but negative for CD34 or CD31.Conclusions In view of a history of recent rapid growth,the presence of hemorrhagic pseudocysts and high vascularity,AFH should be differentiated from angiosarcoma and angiomatoid fibrous histiocytoma.

Objective To assess the clinical and histopathological features as well as treatment of Wells syndrome.Methods The clinical and pathological findings from 7 patients with Wells syndrome were retrospectively reviewed.Results Lesions were located on both lower extremities in 4 patients,on the back in 1 patient,on the face and trunk in 1 patient,and on the buttocks in 1 patient.Clinical manifestations included cellulitis (n =3),urticaria (n =1 ),annular plaques (n =1 ) and papulonodules (n =2).Histopathological examination of skin biopsies showed an infiltrate of numerous eosinophils with occasional flame figures in the dermis of all the patients.Leucocytoclastic vasculitis was found in 3 cases.No triggering factors were found in any of the 7 cases.The lesions nearly subsided in 3 patients after 2-week treatment with oral small-dosage prednisone and tripterygium glycosides.Conclusions Wells syndrome shows a wide diversity of clinical manifestations with distinct histological features.Systemic glucocorticoids and tripterygium glycosides are effective for the control of this condition.