Objective To discuss the efficacy of Baofukang combined with clindamycin injection in treatment of chronic cervicitis patients with high-risk human papilloma virus infection (hr-HPV).Methods Totally 100 patients who had chronic cervicitis with hr-HPV were selected and divided into two groups randomly.The patients in control group (49 cases) were given Baofukang.The patients in observation group (51 cases) were given Baofukang combined with clindamycin injection.The efficacy of Baofukang combined with clindamycin injection in treatment of chronic cervicitis patients with hr-HPV was evaluated by efficacy,HPV negative rate,inflammatory factors before and after treatment and adverse reaction during treatment.Results After treatment,the effective rate of observation group was 92.1％,and the effective rate of control group was 75.5％,the effective rate of observation group was higher than that of control group (P ＜ 0.05).After treatment,the HPV negative rate in observation group was 90.2％,the control group was 59.2％,the HPV negative rate of observation group was higher than that of control group (P ＜ 0.05).Before treatment,there was no statistical significance on hs-CRP,IL-6,and TNF-α between two groups.After treatment,the hs-CRP,IL-6,and TNF-α were decreased in two groups (P ＜ 0.05).The hs-CRP,IL-6,and TNF-α of observation group were lower than those of control group (P ＜ 0.05).There was no statistical significance on adverse reaction between two groups.Conclusion Baofukang combined with clindamycin injection had a good therapeutic effect on chronic cervicitis with hr-HPV.It could inhibit HPV and inflammatory factors well.It was safe and worthy of clinical use.

[Objective] To investigate the effect of external application of osmosis-improving herbal medicine and enema with blood-activating and stasis-removing herbal medicine for chronic pelvic inflammation. [Methods] Single-blind and randomized trial was performed on 120 cases of chronic pelvic inflammation. The cases were randomized to two groups: group A ( n = 57) was treated by enema with blood-activating and stasis-removing herbal medicine and group B ( n = 63) by the combination of enema and external application of osmosis-improving herbal medicine. Fourteen days constituted one course of treatment and the therapeutic effect was evaluated after two courses. [Results] In group A, 30 cases were cured, 21 effective, 6 ineffective and the total effective rate was 89.5%; 43 were cured, 18 effective, 2 ineffective and the total effective rate was 96.8% in group B. The result of Ridit analysis showed that the effect of group B was better than that of group A ( t = 3.08, P

【Objective】To observe the therapeutic effect of oral use of Neiyi Pill(composed of Radix Salviae Miltiorrhizae,Sanguis Draxonis,Rhizoma Sparganii,Rhizoma Curcumae,Semen Persicae,Radix Notoginseng,Radix Angelicae Sinensis,Ramulus Cinnamomi,Rhizoma Cyperi,Radix Achyranthis Bidentatae,etc.) combined with enteroclysis of Neiyi Enema(composed of Radix Salviae Miltiorrhizae,Sanguis Draxonis,Semen Persicae,Rhizoma Curcumae,Radix Paeoniae Rubra,Rhizoma Polygoni Cuspidati,etc.) for dysmenorrhea induced by endometriosis(EMS).【Methods】Fifty-eight patients diagnosed as endometriosis by laparoscopy were divided into 3 groups by randomized single-blind method.Group A(n=16) was treated with Neiyi Pill orally,Group B(n=24) with Neiyi Pill orally combined with enteroclysis of Neiyi Enema and Group C(n=18) with danazol orally.The 3 groups respectively received the corresponding treatment for 3 successive menstrual cycles(MC).Dysmenorrhea scoring was carried out according to the Guide for Clinical Research of New Chinese Herbal Medicine before treatment and after 1,2 and 3 menstrual cycles.The effects on symptoms of dysmenorrhea,lumbosacral discomfort and irritative symptom of rectum,signs of tenderness and nodule in posterior fornix of vagina and adnexal masses,and syndrome of blood stasis were observed.Meanwhile,the adverse reactions were also monitored.【Results】After treatment,effect on relieving dysmenorrhea in group B was superior to that in group C(P0.05).Effect on lumbosacral discomfort and syndrome of blood stasis in groups A and B was superior to that in group C(P0.05).Effect on irritative symptom of rectum,tenderness and nodule in posterior fornix of vagina and adnexal masses in group B was superior to that in group C(P0.05).【Conclusion】Oral use of Neiyi Pill combined with enteroclysis of Neiyi Enema has a better effect for endometriosis than only oral use of Neiyi Pill only or danazol,and has no obvious adverse reactions.

Objective To identify the gene locus and the mutation of DSRAD (double-stranded RNA adenosine deaminase) in a Chinese dyschromatosis symmetrica hereditaria(DSH) family. Methods After confirming the diagnosis of the DSH proband, the genomic DNA was extracted from the whole blood samples of every members of the pedigree. The DSRAD gene intervals were localized by linkage analysis and haplotype reconstruction. The mutation of DSRAD was detected by direct sequencing. Results The candidate gene was localized at the 1q region, consistent with the reported region. The direct sequencing results showed that there was a CAA→TAA transition at exon 2 of DSRAD in all affected family members, which consequently led to a nonsense mutation of Gln517Ter. Conclusion A nonsense mutation is found in the Chinese DSH family.

Objective The interleukin-1β (IL-13) expression in serum of acute myeloid leukemia (AML) patients was evaluated. To explore the significance of IL-1β in leukostasis and tissue infiltration by leukemic cells. Methods ELISA was used to investigate the contents of IL-1β in serum of 83 newly diagnosed with AML which contains 16 hyperleukocytic AML patients, and compared the IL-1β level between the hyperleukocytic AML group and non-hyperleukocytic AML group, the infiltrated group and non-infiltrated group. Results The content of IL-1β in AML serum [(88.23±36.30) pg/ml] was higher than that of in the control group[(29.56±15.53) pg/ml], with significant difference (P ＜0.01). There was a higher level for IL-1β in hyperleukocytic AML group[(136.67±65.68) pg/ml] than in non-hyperleukocytic AML group [(69.85±48.35) pg/ml],and there was a significant difference. IL-13 and peripheral blood cells were in linear correlation (r=0.74, P ＜0.01). There was a higher level for IL-1β in infiltrated group[(111.31 ±57.35) pg/ml] than in the other group [(79.68±43.42) pg/ml], and there was a significant difference. Conclusion The IL-1β may play an important role in leukostasis and tissue infiltration by leukemic cells in AML.

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ObjectiveTo investigate the clinical effect of nape acupuncture on post-stroke dysphagia by a meta-analysis.Method Domestic literature on clinical randomized controlled trials of nape acupuncture treatment of post-stroke dysphagia published from Jun. 2004 to Jun. 2014 were obtained by a computer search ofChina National Knowledge Internet(CNKI), Wanfang Data medical information system (WF), VIP information resource system (VIP) and Chinese Biomedical Literature Database (CBM) combined with a manual search of Jinan University library journal database. Literature inclusion and exclusion criteria were established to extract data and the qualities of the included studies were assessed using a Jadad rating scale. A meta-analysis was made using the software ReviewManager 5.2.ResultA total of 17 articles were included with 1158 patients. The results of meta-analysis showed that there were statistically significant differences in the total efficacy rate [OR=3.99, 95%CI(2.83, 5.63),P<0.00001] and the cure rate[OR=2.67, 95%CI(2.03, 3.53),P<0.00001] between the nape acupuncture and control groups.ConclusionNape acupuncture has clinically a good therapeutic effect on post-stroke dysphagia. Multi-center, large-sample and high-quality studies are still needed for validation because the qualities of the included articles are lower, which is one of the factors influencing the assessment.

Objective To estimate the diagnostic value of interstitial infiltration pattern for early morphea.Methods Twenty-five cases of early morphea pathologically characterized by interstitial infiltration of inflammatory cells were collected from 2010 to 2012.The clinicopathological features of these cases were retrospectively analyzed.Results The average clinical course was 7.5 months.The primary manifestation was edematous dark erythematous plaques,and interstitial or mixed infiltrate of inflammatory cells was the characteristic histopathological presentation.After anti-inflammatory treatment,lesions markedly improved or disappeared in 70％ of these patients.Conclusions Interstitial infiltration of inflammatory cells is a rare histologic pattern in early morphea.To learn and recognize this pattern may be beneficial to the diagnosis and treatment of early morphea.

An 88?year?old man presented with multiple nodules and ulcers on both lower limbs for 6 months. Dermatological examination showed multiple dark purple hard nodules with central crusted ulceration. Histopathological examination revealed a diffuse infiltration of atypical lymphoid cells with large hyperchromatic nuclei in the dermis and subcutis, as well as small necrotic foci in some regions. Conspicuous mitoses were seen. The large lymphoid cells were positive for CD20, CD79a and Bcl?2. Epstein?Barr virus?encoded RNA was detected by in situ hybridization. These findings were consistent with Epstein?Barr virus?positive diffuse large B?cell lymphoma. The patient gave up systemic chemotherapy and died six months later.

Objective To observe the effects of tetramethyipyrazine(TMP) on retina to find out whether it can protect retina from glaucomatous damage. Methods Twenty-four rabbits were randomly divided into four groups. One eye of each rabbit was model eye induced by 2％ methylcellulous, and the other was control eye. Normal saline, TMP, timolol and a combination of timolol and TMP were administrated to group A, B, C and D respectively. At the end of 4th week, eyes were excavated for light and electron microscopic study. Results The numbers of ganglion cells (P ＜0. 01) and bipolar cells (P ＜0. 01) in model eye were different significantly between group A and B. In group A, the model eye ganglion cells were karyopyknosis, chromatin margination and nuclear membrane rupture; some in ner nuclear cells dcveloped marked lytic changes; outer segment appeared disorganized; but group B changed slight ly. Conclusion The results suggest that TMP may protect retina from glaucomatous damage.