Objective To study the reLationship between blood group antibody IgG titer of pregnant women and ABe hemolytic disease of newborn(HDN).Methods Blood group antibody IgG titem of 2700 pregnant women who is O and Rh positive with a non-O husband were detected.After bom,blood groups,Coomb's test,free antibody and antibody release test were done for those 280 babies who were in high risk of HDN.so that we could understand the relationship between the anlibody IgG level of pregnant women and ABO-HDN.Results The percent of anti-A (B)IgG titer＜64,64,128,256 and≥512 in the 2700 O type pregnant women was 62.4%,16.8%,12.6%,5.8%and 2.4%,respectively.There were 67 babies suffered from HDN in those 280 bahies who had high risk.which accounted for 23.9%.With the anti-A (B)IgG liter was 64,128,256 and≥512,the percent of HND positive was 10.2%,27.8%,45.7% and 50.0%,respectively (P＜0.01).Conclusion There is a positive relationship between blood group antibody IgG liter of pregnant women and ABO-HDN.It is necessary for the pregnant women to detect the level of anti-A(B)IgG.

The article summarized recent studies on the composition and activity analysis,as well as the clinical application and mechanism of citrus aurantium and its prescriptions,in an attempt to provide theoretical evidence for the clinical application of citrus aurantium on improving gastrointestinal motility.

Objective To study the clinical application value of detection of IgA content in blood recipients before transfusion .Methods 300 blood recipients were selected as the research subjects ,and the content of IgA was detected before blood transfusion .Results Through the analysis of 300 blood recipients,IgA content of 5 cases was lower than the normal reference value ,the incidence rate of IgA deficiency or low was 1.7%;the IgA content of other 295 cases was in the normal reference value ,the normal rate of IgA was 98.3%.5 blood recipients with IgA deficiency or low were associated with hypersensitivity reactions ,the incidence rate was 100%.Of 295 cases with normal content of IgA,2 cases associated with hypersensitivity reactions ,the incidence rate was 0.7%,the difference between the two groups was statistically significant (χ2 =7.45,P<0.05).Conclusion Determination of IgA content in blood recipi-ents before blood transfusion can effectively guide the correct transfusion ,reduce and prevent the occurrence of hyper-sensitivity reactions of transfusion ,improve the safety of blood transfusion ,which has important application value in clinical practice .

Objective To investigate the clinical significance and application value of the irregular antibody test before transfusion.Methods The irregular antibody test was conducted in 2 500 patients before blood transfusion.Results Through the analysis of irregular antibody examination of 2 500 patients with blood transfusion,15 cases appeared irregular antibodies in the serum,the positive rate was 0.60％.The positive rate of male irregular antibody was 0.12％ (3/2 500),the positive rate of female irregular antibody was 0.48％ (12/2 500).The positive rate of female irregular antibody was significantly higher than that of male irregular antibody (x2 =7.34,P ＜ 0.05) ; The irregular antibodies were mainly anti C,anti D,anti E and anti M type and non-specific antibody,the proportions were 13.3％,26.7％,20.0％,33.3％,6.7％.The incidence rate of blood transfusion hypersensitivity reactions in irregular antibody positive donor was 100.0％,that in irregular antibody negative patients was 0.0％,the difference between the two groups was statistically significant (x2 =9.28,P ＜ 0.05).Conclusion Irregular antibody test in patients before blood transfusion can effectively guide the patients to correct blood transfusion,and can effectively prevent and reduce blood transfusion hypersensitivity,improve the safety in the process of blood transfusion.

Mild Cognitive Impairment(MCI)is a transitional stage during the progress of cognitive Impairment. Through the analysis of the relativity of apolipoprotein E(apoE),apoE genetic polymorphism,phlegm stagnation and blood stasis,hyperlipemia and MCI,we got this conclusion:syndrome of phlegm stagnation and blood stasis is an important underlying pathogenesis of MCI:the syndrome of phlegm stagnation and blood stasis has a close relation to apoFe4 allele,and lipid-metabolism disturbance is a key link to this relation.

Objective To investigate the effect of Naloxone in the pre-hospital care of AOPP.Methods 54 patients with the pre-hospital care of AOPP were divideded into two groups.The treatment group was treated with Naloxone,Atropine and Pyridine aldoxime methyliodide,the control group treated with Atropine and Pyridine aldoxime methyliodide,and the results were analyzed using t-test and x2 test.The efficacy of the two groupswas compareded.Results In the treatment group,the mumber of drug delivery and the dosage of Atropine were less.The recovery time of the actirity of Cholinesterase and the patients coma were shorter.Complications and mortality was significantly lower,but the cure rate was significantly higher than that of the control group.Conclusion Naloxone is effective and safe in the treatment the pre-hospital care of AOPP.It is worthy of clinical promotion.

Introducing the current challenges of library of Institute of Information Research, China Rehabilitation Research Center,the paper puts forward the establishment of effective literature resources construction and reader service mechanism. Construction of library collection should be developed from two aspects, namely, the acquisition and integration parts, so as to develop characteristic,compound collections with both printed literatures and digital literatures. Reader service is not only including the traditional service but also covering deep-rooted professional information analysis and multiple types of services.

Objective:To investigate therapeutic effect and toxici ti es of neoadjuvant chemotherapy(NACT) with Carboplatin(CBP) and 5-Fluorouracil (5-FU) for the patients with oral squamous cell carcinoma(OSCC)by chronomodul ated administration. Methods:73 patients with T3 and T4 OSCC, ad mitted from March 2002 to April 2004, were randomly divided into two groups,37 c ases were in chrono-chemotherapy group (groupⅠ) and 36 cases in routine-chemo therapy group (group Ⅱ). Therapeutic effects and side effects between tw o groups were compared.Results:After two NACT courses, the effec tive rate in group Ⅰ and group Ⅱ was 75.68% and 52.78%respectively (P

Purpose:To further investigate the role of the novel negative immuno-regulatory molecules in tumor evasion by testing B7-H4 e xpression on tumor tissues and spleen macrophages induced by tumor antigen of mi ce and BTLA expression on T cells of the tumor bearing mice. Methods:Via RT-PCR, we evaluated the differences in the expres sion of B7-H4 mRNA of the tumor cell lines (3LL and Renca) in vitro,tumor t issues and the expression of BTLA mRNA of the tumor bearing mice T cells;via flo w cytometry, we evaluated the differences in the expression of B7-H4 surface pr otein of the spleen macrophages from tumor bearing mice and the normal spleen ma crophages after stimulation with the tumor antigen. Moreover, via immunohistoche mistry , we also evaluated the differences in the expression of B7-H4 surface p rotein of tumor tissues. Results:Tumor cell lines (3LL and Renca) did not express the B 7-H4 mRNA in vitro.All tumor tissues or spleen macrophages in tumor bearing mice have a high expression of both B7-H4 mRNA and B7-H4 protein. Compared wi th those from normal mice, T cells from tumor bearing mice showed a higher expre ssion of BTLA. Stimulated by tumor antigen in vitro, normal mice spleen macr ophages B7-H4 expression increased significantly; while no apparent difference was found in the groups stimulated by the corresponding normal tissue antigen. Conclusions:The aberrant expression of the costimulatory molecu les, B7-H4 and its receptor BTLA, may have a regulative effect in tumor evasion immunity.

Objective To analyse the mutation types of the NOTCH3 gene with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy(CADASIL). Methods Including 4 probands of CADASIL,10 cases of CADASIL and 4 normal members from 3 CADASIL families and 100 healthy controls were recruited. Genomic DNA was extracted from white blood cell. The amplicons were analyzed by the denaturing high-performance liquid chromatography (DHPLC) technique. The positive samples which identified by the DHPLC were sequenced to determine the specific mutation or polymorphism, respectively. Results Three heterozygous missense mutations including Cys90Arg, Arg141Cys, Cys134Tyr located in the exon3 and exon4 were found in the 4 probands and 10 cases of CADASIL among the 3 families. 15 polymorphisms were also found. 2 members individual from family 1 and 2 were found to carry the same pathological mutations as in their proband but without clinical symptoms. They were identified as preclinical patients. Conclusions Mutation detection of NOTCH3 is the molecular genetic mechanical for CADASIL. The exon3 and exon4 are possible hot mutation spots in Chinese patients. The mutation of Cys134Tyr in exon4 is a novel mutation which has not been reported previously in China.