Tumor stem cells have abilities of high proliferation,serf-renewal and multi-directional dif-ferentiation ;which is closely responsible for tumor' s survival and recurrence. A minor portion of myeloma stem cells exist in multiple myeloma. The research of myeloma stem cells' origins, phenotypic analyses, signaling pathways, drug resistance and other biological characteristics will do great efforts to the effective therapy of mul-tiple myeloma.

Objective To investigate the clinical significance of serum lactic dehydrogenase (LDH)alteration in patients with acute leukemia (AL). Methods Serum LDH of 156 AL patients including untreated 63,completely remission (CR) 46 and relapsed 47 were measured by biochemistry analyzer. R banding technique was used for karyotype analysis this three groups were divided according to poor, good and normal prognostic chromsome. The correlation between LDH level with AL periods, chromsome groups, peripheral WBC counts and leukemia subtypes were analysed. Results The LDH level of AL patients untreated (P50 399 U/L) and relapsed (P50 265 U/L) were significantly higher than those CR (P50 153 U/L), P ＜0.05. LDH level were closely correlated with peripheral WBC counts (rs=0.604) and leukemia cells of bone marrow (rs=0.538, both P ＜ 0.01), and both were also correlated with leukemia subtypes (LDH in subtypes L2, M4 were higher than other subtypes of AL, P ＜ 0.01). The refractory and relapsed AL patients had higher LDH level (P50 538 U/L) than the others (P50 294 U/L) when they were diagnosed. And those with poor prognostic chromsome also had higher LDH level (P50 778 U/L) than those with good (P50 306 U/L) and normal prognostic chromsome (P50 405 U/L, P ＜0.01). Conclusion LDH level increased obviously in untreated AL patients and decreased in CR ones. It was correlated with different periods, subtypes and grade malignancy of AL So LDH level can be considered as an important indicator for therapeutic effects and prognosis for AL patients.

ObjectiveTo measure levels of serum resistin in patients with nonalcoholic fatty liver disease (NAFLD) and explore the relationship between serum resistin and insulin resistance,and the role of serum resistin in the pathogenesis and progression of NAFLD.Methods 53 patients (including 30males and 23 females) with NAFLD and the 28 control subjects (including 16males and 12females) were enrolled in this study.The height,weight,waist circumstance,hip girth and blood pressure of all subjects were obtained by standard methods.To all the subjects,the parameters including fasting blood glucose (FBG),triglyceride ( TG),total cholesterol (TC),high-density lipoprotein cholesterol ( HDL-c ),lowdensity lipoprotein cholesterol ( LDL-c ),alanine aminotransferase ( ALT),aspartate aminotransferase (AST)and gamma glutamyl transferase (GGT) were measured.Serum resistin was measured by electrochemiluminescence immunoassay (ECLIA) and fasting serum insulin (FINS) were measured by enzymelinked immunosorbent assay (ELISA).Body mass indexes (BMI),waist hip ratio (WHR),body fat percentage ( BF％ ) were calculated with formula.Homeostasis model assessment was applied to assess the status of insulin resistance index (HOMA-IR).The relationships between serum resistin and various parameters in patients with NAFLD were observed and analyzed by statistical methods.ResultsThere were no significant differences in the number of cases,average age,compositions of sex and BF％ between the NAFLD group and the control group ( P ＞ 0.05).In the NAFLD group,systolic blood pressure ( SBP),diastolic blood pressure(DBP).The waist circumference,hip girth and BMI and WHR were higher than those of control group ( t =3.54,3.32,3.56,5.85,5.56,4.10,P =0.001).There were no significant differences on the level of TC,HDL-c,LDL-c between the NAFLD group and the control group ( P ＞0.05).In the NAFLD group,FBG,TG,AST were higher than those of control group ( P =0.001,P =0.004,P =0.001 ).The levels of serum resistin,FINS,HOMA-IR,ALT and GGT in NAFLD group were significantly higher than those of control group,and there was significant difference between two groups ( P =0.0005).There was no significant correlation between serum resistin and age,BMI,WHR,BF％,FBG,TG,TC,HDL-C,LDL-C,AST in NAFLD group ( P ＞ 0.05).The levels of serum resistin in patients with NAFLD were positively correlated with GGT,ALT,FINS,HOMA-IR ( r =0.354,0.391,0.875,0.881,P ＜0.05 or ＜0.01 ).After multiple stepwise regression analysis,the results showed that HOMA-IR was the most important factor for affecting the levels of serum resistin.The levels of serum resistin in male and female patients with NAFLD were higher than those of control group ( P ＜0.05).But no statistical difference was found between males and females in two groups ( P ＞ 0.05).ConclusionsThe levels of serum resistin in patients with NAFLD were significantly higher than those of controls.Serum resistin in patients with NAFLD was closely correlated with insulin resistance,and it may participate in the pathogenetic progress of NAFLD.Serum resistin in patients with NAFLD might be associated with hepatic insulin resistance,and it has no correlations with obesity-related insulin resistance.Serum resistin in patients with NAFLD may participate in the inflammation of fatty liver disease as inflammatory cytokines.

ObjectiveTo investigate and analyze the prevalence and risk factors of acute stress disorder (ASD) among survivors of road traffic accidents.MethodsA total of 206 participants ( 168 men,38 women) were administrated with the acute stress disorder scale (ASDS) 2 to 23 days after the traffic accidents.ResultsThere was a total of 61 (29.6％) possible ASD patients among all participants.Women had more serious ASD symptoms than men (male:36.70 ± 11.42; female:41.18 ± 12.58; P＜ 0.05 ),the married's ASD symptom severity was higher than the single's ( married:38.37 ± 11.94 ; single/widowed/divorced:30.86 ± 8.37 ; P ＜ 0.01 ),people with low degree of education had more severe ASD symptoms than the people who had high degree of education (P＜0.05) ; being elder( r=0.43,P＜0.01 ),having fear / helpless / horror experience of accident ( r=0.21,P＜ 0.01 ) were significantly correlated with the severity of ASD symptoms.According to linear regression analysis,age could positively predicted ASD symptoms ( β =0.29,P ＜ 0.01 ) too.ConclusionASD is a common mental problems among Chinese survivors of road traffic accident,and the main predictive factors of ASD include gender,age,marital status,the degree of education,whether there is fear / helpless / horror experience of accident.

Objective To study the characteristics of self-concept and personality in the SOS children vil-lage's children. Methods Sixty-one children from SOS children village, sixty-one children with single parent and sixty-one children in intact family, matched with the SOS children in gender and age, were assessed by Piers-Harris children's self-concept Scale(PHCSS), Eysenck Personality Questionnaire for Children (EPQ) and general status questionnaire. Result In the PHCSS, the scores on behavior, body of color and attribute, blessedness and satisfac-tion and the total score of boys in the SOS group were lower than those in the intact family group's ((10.69± 2.74) vs (12.57±2.36),P=0.013;(6.51±2.63) vs (8.29±2.75), P=0.011;(6.54±1.93) vs (7.97± 1.60), P=0.004; (48.09±10.88) vs (55.86±10.11), P=0.007)). The E scale's score of the SOS group's girls was lower than that in the intact group (P=0.004). Children's self-concept status was related with their learning environment, family structure, parent' s learning expectation, daily communication, personality, number of good friends and academic performance (-0.566 < r < 0.395). Conclusions The self-concept status of girls in the SOS children village show lower level. The personality of boys in the SOS children village tend to be introver-sive and stable. Children's self-concept status was related with the factors of their personality, life environment,family structure,et al.

Objective To investigate the features of patients with cyclic citrullinated peptide (CCP) antibodies in primary Sj(o)gren's syndrome (pSS) with the retrospective method.Methods Seventy eight pSS patients were studied.Enzyme-linked immunosorbent assay (ELISA) indirect immunofluorescence was used to measure anti-CCP antibodies.The patients with and without anti-CCP antibodies were correspondingly classified as autoantibody-positive and negative pSS groups.Clinical and laboratory features were compared between autoantibody-negative and positive group.Results (1) Seven (9.0％) of pSS patients had positive anti-CCP antibody.No significant difference was found in sex,ages,and disease course between autoantibody-nagative and positive pSS patients.(2) The anti-CCP-positive pSS was associated with the rates of decayed tooth,fatigue,dry mouth,dry eyes,and tender of joint (P ＜ 0.05).Whereas,the antiCCP-negative pSS was not significantly related to fever and mumps (P ＞ 0.05).(3)From serologic characteristics aspects,the anti-CCP-positive patients showed higher level of tender of joint than the anti-CCP-negative patients.No significant difference was found in white blood cell (WBC),hemoglobin (HGB),platelet (PLT),erythrocyte sedimentation rate (ESR),C-reactive protein (CRP),immunoglobulin A (IgA),IgG,IgM,and rheumatoid factor (RF) between two groups (P ＞ 0.05).Conclusions The anti-CCP-positive patients were 9％ ofpSS patients,and were more likely to occur tender of joint with higher level of tender of joint.

Objective To investigate couple's personality similarity effect on the marital quality,and to explore the vairance of such effect under different levels of spouse's depression. Methods The cross?sectional da?ta of 4644 couple from the national survey of mental health database in china( 2009) was used. Couples completed Locke ?Wallance marital adjustment test,The CES?D scale a self?report depression scale and Eysenck personality questionnaire short scale for Chinese version (EPQ?RSC). Results The personality similarities of couples(0.44 ±0.27) and their marital quality (wife (103.71±27.54),husband (106.15±26.94))had significant positive corre?lation ( r=0.25~0.27, P<0.01). Regression analysis showed the effect of personality similarity on the marital quality were varied according to the level of spouse depression( β=0.09?0.22, P<0.05) . Among participants whose husband or wife had higher level of depression,couple's personality similarity had stronger effect on his/her per?ceived marital quality. Conclusion Husband and wife personality similarity is related to marital quality signifi?cantly,and the spouse depression plays a significant moderating role in this relationship.

Objective To investigate the changes of intestinal mucosal barrier function and protection of alanyl-glutamine(Alm-Gln) dipeptide during early stage of postthoracotomy in aged patients.Methods A prospective,randomized and controlled trial was conducted and 20 aged patients who underwent non-digestive thoracotomy were randomized into two groups,experimental group (intravenous administration of Aln-Gln dipeptide,0.5 g/(kg · d),for 4 days,n =10) and control group(equal amount saline as placebo,for 4 days,n =10).The indices of boby temperatures,heart rates,respiration and white blood cell count of all patients were daily recorded during administration.Serum concentrations of glutamine (Gln),D-lactate,diamine oxidase (DAO) and tumor necrosis factor-α(TNF-α) were measured before and after operation.Results There were no statistically significant differences in the patients' general information between experimental group and control group including age,gender and body weight.Plasma Gln concentration in postoperative 5 days was higher than that of pre-operation of experiment group ((478.32 ± 47.42) μmol/L vs.(372.67 ± 29.14) μmol/L,P =0.021).The plasma Gln level of control group at 5th day after operation was higher than that in pre-operation ((431.12 ± 42.27) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.017).The plasma level of Gln in experimental group was significantly higher than that in control group after operation((478.32±47.42) μmol/L vs.(386.29 ± 19.73) μmol/L,P =0.012).There were no significantly differences between the two groups in terms of the plasma level of DAO and D-lactate before operation (P ＞ 0.05).Meanwhile the levels of DAO and D-lactate in both group at 5th day after operation were significantly higher than that at before operation(DAO:(2.53 ±0.47) U/ml vs.(1.66±0.32) U/ml,P =0.003;D-lactate:(6.82 ±1.91) mg/L vs.(4.92 ±1.57) mg/L,P =0.024),and the levels of them in experimental group were significantly lower than that in control group(DAO:(1.10 ± 0.23) U/ml vs.(2.53 ± 0.47) U/ml,P =0.013 ; D-lactate:(4.87 ± 1.33) mg/L vs.(6.82 ± 1.91) mg/L,P =0.019).The concentration of TNF-α was significant increase in both two groups at first day after operation,but decreased at the third day.The concentration of TNF-α in experimental group at 5th day after operation was lower than that in control group ((6.89 ± 5.21) pg/L vs.(13.04 ± 4.46) pg,/L,P =0.003).The morbidity of systemic inflammatory response syndrome (SIRS) was significantly decreased in experimental group and the rate of SIRS was also lower than that in the control group(P ＜ 0.01).Conclusion Intestinal mucosal barrier function was damaged after thoracotomy in aged patients.Administration of Aln-Gln dipeptide could increase the level of serum Gln,protect the intestinal barrier and attenuate the systemic inflammatory response.Aln-Gln dipeptide can be used to help aged patients recover rapidly.

Objective To explore the clinical effect of small cell lung cancer(SCLC) treatment with recombinant human endostatin injection and cis-platinum complexes.Methods 100 patients with SCLC were divided into 2groups.The experiment group used cis-platinum complexes while the control group used recombinant human endostatin injection.Results The effective rate of experiment group was obviously higher than the control group ( 74.0％ vs60.0％,x2=2.16,P ＜ 0.05 ).The median survival time of experiment group was better than control group( t=2.02,P ＜ 0.05 ).The diverse of untoward effect of two groups had no difference ( P ＞ 0.05).Conclusion Recombinant human endostatin injection and cis-platinum complexes were better therapy for SCLC,which had stable effect,no severe untoward effect and overcome the drug-fast of cis-platinum complexes.

Objective To study the urodynamic characteristics of women with pelvic organ prolapse in prolapse and reset condition. Methods From Jan. 2010 to Apr. 2010, 30 patients with pelvic organ prolapse (POP), including 1 case in grade Ⅰ , 6 cases in grade Ⅱ , 21 cases in grade Ⅲ and 2 cases in grade Ⅳ, treated in Peking University People's Hospital were analyzed their urodynamical changes at status of prolapse and reset with pessary. Results (1)The symptoms: in the prolapse condition, there were 11 patients with bladder outlet obstruction( BOO), 7 patients with residual volume more than 100 ml, and 4 patients with stress urinary incontinence(SUI). In the reset condition, there were 5 patients with BOO,5 patients with residual volume more than 100 ml, and 4 SUI patients. (2) Characteristics of urodynamic:there were significant difference in first desire to void ( FD, P = 0. 047), normal desire to void ( ND, P =0. 007), strong desire to void ( SD, P = 0. 001 ), maximum cystometric capacity ( MCC, P = 0. 001 ),maximum flow rate ( Q=max, P = 0. 001 ), average flow rate ( Q P = 0. 001 ), and residual volume ( RV,P = 0. 025 ) between prolapse and reset condition among all patients. In patients with grade Ⅰ to Ⅱprolapse, Qmax were (11 ±6) ml/s in prolapse condition and (15 ±4) ml/s in reset condition (P =0. 014), Qave were ( 6 ± 4 ) ml/s in prolapse condition and ( 7 ± 3 ) ml/s in reset condition ( P = 0. 237 ),RV were (29 ±46) ml in prolapse condition and (15 ±25) ml in reset condition (P =0. 157). Among patients with grade Ⅲ to Ⅳ prolapse, Qmax were ( 11 ± 8 ) ml/s in prolapse condition and ( 17 ± 10) ml/s in reset condition (P=0.001), Qave were (5 ±4) ml/s in prolapse condition and (7 ±4) ml/s in reset condition ( P = 0. 002 ), RV were ( 105 ± 169 ) ml in prolapse condition and (47 ± 92) ml in reset condition (P = 0. 037 ). Conclusions Patients with pelvic organ prolapse Ⅲ - Ⅳ may present pseudo BOO,recessive stress urinary incontinence and residual volume more than 100 ml. There were significant improvement in cystometric sensation, capacity, flow rate, and residual volume after reset. The urodynamic test for patients with pelvic organ prolapse in the reset condition might be much better in evaluating lower urinary tract function.