In recent years,premature nutritional problems is newborn pediatric paramount concenrn problem.For three material(glucose,protein and fat)metabolism and utilization.premature infants has its own characteristics.In order to avoid side effect,classic parenteral nutrition plan provide for fat emulsion and amino acid gradually,but make premature infants in poor nutrition state.Recent clinical studies have shown that early nutrition is particularly important.Premature infants bom in 24 hours has been given full amount vein nutrition could provide good basis of energy.and avoid associated complications.

New nano-fluorescent quantum dots (QD) have excellent optical properties, enabling them widely used in clinical cancer research. When combined with a variety of ligands, quantum dots have been successfully used for early detection of primary tumor, angiogenesis and metastasis, all of which have opened up a new frontier in early diagnosis of cancer.

Objective To study the value of digital molybdenum target mammography in diagnosing breast cancer.Methods Digital molybdenum target X-ray findings of 36 cases with breast cancer confirmed by pathology were retrospectively analysed.Results X-ray appeared as masses in 22 cases(61.1%),masses with calcification in 7(19.4%),asymmetry increased density with structural disorder in 4(11.1%),Pagets disease in 1(2.7%),pure calcification in 3(8.33%),abnormal vessels in 3(8.33%),skin thickening in 4(8.73%).Conclusion The direct and indirect digital molybdenum target mammographic signs of breast cancer are important for early diagnosis and differential diagnosis of breast cancer.

Objective To observe the clinical efficacy of electrothermal needle apparatus in treating mild hip osteoarthritis.Method Ninety patients were divided into a control group and a treatment group by using the random number table, 45 cases in each group. The treatment group was intervened byelectrothermal needleapparatus; the control group was treated with ordinary electroacupuncture plus TDP radiation. After 15-day treatment, the therapeutic efficacies of the two groups were evaluated by using Visual Analogue Scale (VAS) and Japanese Orthopaedic Association (JOA) scores.Result The VAS and JOA scores were improved inthe two groups after the treatment (P<0.05), and the improvements in the treatment group were more significant than those in the control group after the 15-day treatment (P<0.05).Conclusion Electrothermal needle apparatus can produce a significant therapeutic efficacy in treating mild hip osteoarthritis.

Objective Clinical reports on perilymphatic fistulae(PLF) of the round window membrane(RWM) have shown different degrees of hearing loss. For the unknown mechanisms of hearing loss, the morphological changes of cochlea with perilymphatic fistulae(PLF) was studied.Methods Among 10 guinea pigs, each ear on one side was punctured and the ear of another side was used as control. 4 hours after operation, the morphological changes of cochlea were observed.Results It was found that 2 ears with endolymphatic hydrops, 1 ear with strica vascularis stripped, venous hyperemia and hemorrhage in cochlea, 7 ears with no changes.Conclusion The study suggestes that the normal and the abnormal morphological changes of cochlea can all be seen after the rupture of RWM.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

Objective To evaluate the effects of different optional time leading to complication and prognosis by laparoscopic cholecystectomy.Methods Search all of clinical randomized trials on early and delay laparoscopic cholecystectomy in CNKI database.The RevMan 5.0 software was used to conduct the Meta-analysis,which was carried out by collecting information based on inclusion and exclusion criteria from all trails' information available,and the pubhcation bias analysis and the susceptivity analysis was also evaluated to make sure the results stable.Results There was significant statistical difference in conversion,surgery time,complication and postoperative hospitahzation time between the ＜ 72 h group and ＞ 72 h group (P ＜ 0.05).Conclusions Meta-analysis shows that in the laparoscopic cholecystectomy progress,the surgical outcome is best with 72h,which can short the surgery and postoperative hospitalization time,reduce the complication and decline the conversion.

OBJECTIVE To investigate the tinnitus' prevalence in Healthy check-up population in Dalian City. METHODS 1748 cases (male 806, female 942) were chosen from the Physical Examination Center of our hospital from February 1st, 2015 to April 1st, 2015, the tinnitus questionnaire was performed. For the cases with the tinnitus symptoms, the characteristics of tinnitus were further inquired, including the course of tinnitus, seizure frequency, the duration of tinnitus, pulsatile or persistent, unilateral or bilateral. RESULTS The total prevalence of tinnitus is 32.4%(566/1748), the prevalence of persistent tinnitus is 18.3%(320/1748), and the transient tinnitus is 14.1%(246/1748); The prevalence of tinnitus in female are 36.4%(343/942), 20.0%(188/942) and 16.5%(155/942) in turn, which are higher than that of the male 27.7%(223/806), P<0.01; 16.4%(132/806), P =0.054, and 11.3%(91/806), P<0.01. Both the total and persistent tinnitus prevalence increase with age(P<0.01), but the transient tinnitus doesn't increase with age. The prevalence of objective and subjective tinnitus are 1.3%(6.9% out of total prevalence of tinnitus), and 17.0%(93.1% out of total prevalence of tinnitus), respectively. The rate of bilateral tinnitus is 78.1% out of total tinnitus, and rate of unilateral tinnitus is 21.9%(right 11.6%, left 10.3%). In addition, the rates of acute tinnitus, subacute tinnitus, and chronic tinnitus are 4.4%, 3.8%, and 91.9% out of total, respectively. Otherwise, the rates of sleep disorders and hearing loss complaining accounted for 31.9% and 14.1% respectively. CONCLUSION The prevalence of tinnitus is high in healthy check-up population of Dalian, the total prevalence of tinnitus is 32.4%, mainly bilateral,chronic and subjective.

Objective To study the chemical composition of traditional Chinese medicine Xiasangju compound extract.MethodsThe ethanol extract of different organic solvent extraction,silica gel column chromatography,chromatography separation and purification technology for the separation of compounds,the structures were elucidated by chemical and spectral data.ResultsSix fiavonoid components were isolated from the ethyl acetate extract,respectively, kaempferol( Ⅰ ),quercetin( Ⅱ ),luteolin( Ⅲ ),quercetin-3-O-β-D-glucoside (Ⅳ),luteolin-7-O-β-D-glucoside ( Ⅴ ) and rutin (Ⅵ).ConclusionThe obtained compounds were isolated from compound Xiasangju for the first time.

Purpose:To further investigate the role of the novel negative immuno-regulatory molecules in tumor evasion by testing B7-H4 e xpression on tumor tissues and spleen macrophages induced by tumor antigen of mi ce and BTLA expression on T cells of the tumor bearing mice. Methods:Via RT-PCR, we evaluated the differences in the expres sion of B7-H4 mRNA of the tumor cell lines (3LL and Renca) in vitro,tumor t issues and the expression of BTLA mRNA of the tumor bearing mice T cells;via flo w cytometry, we evaluated the differences in the expression of B7-H4 surface pr otein of the spleen macrophages from tumor bearing mice and the normal spleen ma crophages after stimulation with the tumor antigen. Moreover, via immunohistoche mistry , we also evaluated the differences in the expression of B7-H4 surface p rotein of tumor tissues. Results:Tumor cell lines (3LL and Renca) did not express the B 7-H4 mRNA in vitro.All tumor tissues or spleen macrophages in tumor bearing mice have a high expression of both B7-H4 mRNA and B7-H4 protein. Compared wi th those from normal mice, T cells from tumor bearing mice showed a higher expre ssion of BTLA. Stimulated by tumor antigen in vitro, normal mice spleen macr ophages B7-H4 expression increased significantly; while no apparent difference was found in the groups stimulated by the corresponding normal tissue antigen. Conclusions:The aberrant expression of the costimulatory molecu les, B7-H4 and its receptor BTLA, may have a regulative effect in tumor evasion immunity.