Objective To summarize the care measures of the cryosurgical treatment of primary liver cancer,and provide a theoretical basis for clinical treatment and care.Methods A retrospective analysis of 196 cases of primary liver cancer admitted in our hospital from August 2011 to October 2014 including clinical manifestation of adverse reactions and corresponding nursing care was made.Results Postoperative complications of the patients were rectified,and primary symptoms were also relieved.Conclusions Effective nurse care could reduce the incidence of postoperative complications of cryosurgical treatment for primary liver cancer patients,and improve patients' recovery.

Objective To study the influence of pine-seed oil upon body weight,blood lipid and lipid peroxidation of mice fed on high fat feed.Method The mice were fed by high fat feed(80% basal feed added 20% lard,HF) and HF added pine-seed oil(80% high fat feed added 20% pine-seed oil,SHF).At 45 d and 90 d,the body fat content,blood lipid level,and malonyldehyde(MDA),lipofusin(LP),catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) of brain and liver homogenate were measured respectively.Results Pine-seed oil could reduce the body weight serum triglyceride(TG),total cholesterol(TC),low density lipoprotein-cholesterol(LDL-C) content,but increase high density lipoprotein-cholesterol(HDL-C) of the mice fed by HF.It suggested that pine-seed oil had definite effect on regulating blood lipid.Compared with normal control(NC) group,the activities of CAT、T-SOD、GSH-Px of brain and liver in HF group were remarkably lower,but MDA and LP remarkably higher.It suggested that HF could promote lipid peroxidation in brain and liver of mice.Compared with HF group,the activites of CAT,T-SOD and GSH-Px of brain and liver in SHF group were remarkably higher,but MDA remarkably lower.Conclusion Pine-seed oil can reduce the body weight,blood lipid,and lipid peroxidation of mice fed by HF.

Objective To understand antimicrobial resistance of Enterobacteriaceae strains and distribution charac-teristics of carbapenemase-resistant Enterobacteriaceae(CRE)in Xijing Hospital in 2015,so as to provide basis for rational use of antimicrobial agents in clinic. Methods Specimens from infected inpatients and outpatients in Xijing Hospital in January-December 2015 were performed pathogenic culture,isolation and identification. Antimicrobial susceptibility testing was performed with Kirby-Bauer method,carbapenemase confirmatory test was conducted with modified Hodge test,antimicrobial resistance of Enterobacteriaceae was statistically analyzed. Results A total of 4166 pathogenic strains were isolated in 2015,1554 (37.30% )of which were Enterobacteriaceae strains,the top 5 isolated pathogens were Escherichiacoli(E. coli),Klebsiella pneumoniae(K. pneumoniae),Enterobactercloacae (E. cloacae),Proteusmirabilis,and Enterobacteraerogenes. Of 787 E. coli strains,581(73.82% )were extend-ed-spectrumβ-lactamases(ESBLs)-producing strains;of 367 K. pneumoniae strains,182 (49.59% )were ESBLs-producing strains. E. coli and K. pneumoniae had the highest resistance rates to cefazolin,which were 93.14% and 78.48% respectively. A total of 81 (5.21% )CRE were detected,K. pneumoniae,E. cloacae,and E. coli were 41 ,27,and 13 strains respectively;strains were mainly isolated from patients in departments of neurosurgery(n=42),gastroenterology(n= 9),and neurology(n= 8). 1.02% (8/787)of E. coli and 3.27% (12/367)of K. pneu-moniae were multidrug-resistant. Conclusion Enterobacteriaceae accounted for a higher proportion of pathogenic bacteria causing HAI,the detection rate of ESBLs-producing strains remains high,the isolation rate of carbapenem-resistant Enterobacteriaceae is higher than that of last year,especially K. pneumoniae.

OBJECTIVE:To observe the efficacy of levoamlodipine besylate combined with piracetam for vascular dementia(VD).METHODS:66 patients with VD were enrolled:33(treatment group) were assigned to receive Levoamlodipine besylate 2.5～5 mg q.d plus piracetam 0.8 g tid for 3 months,and another 33(control group) to receive piracetam 0.8 g tid plus basic treatment based on patients' blood glucose,blood pressure and blood lipid levels.The patients were given 3-month follow-up.Mini-Mental State Examination(MMSE),Activity of Daily Living Scale(ADL),Hasegawa Dementia Scale(HDS) and Wechsler Memory Scale(WMS) were used to evaluate the patient's cognitional function and mental state before and after treatment.RESULTS:MMSE,ADL,HDS,and WMS scores after treatment were(22.48?3.25),(25.16?8.96),(23.14?2.91),(71.14?19.01),respectively in treatment group versus(20.56?3.18),(29.74?13.01),(20.41?6.25),(60.35?20.60),respectively in control group.The treatment had a better improvement in symptoms than control group did and there was statistical difference between groups(P

Cytotoxic substances and ionizing radiation can easily induce DNA damage, and double strand breaks (DSBs) are the main form of DNA damage. DNA damage can activate intracellular DNA damage responses and further induce related biological effects, such as DNA damage repair and cell cycle arrest. Homologous recombination (HR) is the primary DSB repair mechanism in eukaryotes. Abnormal expression of Rad51C, which is a key factor in the HR pathway, may result in DNA repair disorder, genomic instability, and eventually lead to tumor formation. In recent studies, researchers considered Rad51C as a potential target for cancer treatment. We reviewed the research progress on Rad51C in DNA damage repair and radiotherapy.

Objective To investigate couple's personality similarity effect on the marital quality,and to explore the vairance of such effect under different levels of spouse's depression. Methods The cross?sectional da?ta of 4644 couple from the national survey of mental health database in china( 2009) was used. Couples completed Locke ?Wallance marital adjustment test,The CES?D scale a self?report depression scale and Eysenck personality questionnaire short scale for Chinese version (EPQ?RSC). Results The personality similarities of couples(0.44 ±0.27) and their marital quality (wife (103.71±27.54),husband (106.15±26.94))had significant positive corre?lation ( r=0.25~0.27, P<0.01). Regression analysis showed the effect of personality similarity on the marital quality were varied according to the level of spouse depression( β=0.09?0.22, P<0.05) . Among participants whose husband or wife had higher level of depression,couple's personality similarity had stronger effect on his/her per?ceived marital quality. Conclusion Husband and wife personality similarity is related to marital quality signifi?cantly,and the spouse depression plays a significant moderating role in this relationship.

[ABSTRACT]AIM:Tostudytheeffectsofbaicalin(BC)onglialfibrillaryacidicprotein(GFAP)andnuclear factor-κB ( NF-κB) expression and neuronal apoptosis in juvenile rat hippocampus after status convulsion ( SC) .METH-ODS:One hundred and ninety five juvenile male Sprague-Dawley rats were randomly divided into 3 groups:normal saline pretreatment group (NS group), SC group and SC with BC pretreatment group (BC group).Each of these 3 groups would be subdivided into 5 subgroups sacrificed at 4 h, 12 h, 24 h, 48 h and 72 h after SC.The rat SC model was prepared by lithium-pilocarpine chemical method .The protein expression of GFAP and NF-κB was detected by the method of immuno-histochemistry .The mRNA expression of GFAP was detected by RT-PCR.The neuronal apoptosis was observed by TdT-mediated dUTP nick end labeling ( TUNEL ) .RESULTS: Compared with NS group , the GFAP positive cells was in-creased in SC group (P<0.05).Compared with SC group, the expression of GFAP was significantly reduced in BC group (P<0.05).Compared with NS group, the NF-κB positive cells was increased in SC group (P<0.05).Compared with SC group, the expression of NF-κB was significantly reduced in BC group .RT-PCR showed that the expression trend of GFAP mRNA was similar to that of the protein .Compared with NS group , the TUNEL positive cells in the hippocampus CA1 area in SC group increased significantly 12 h after SC (P<0.01), and reached a peak at 48 h.After the intervention with BC, the TUNEL positive cells decreased significantly between 12~48 h after SC (P<0.05 or P<0.01), but the number of TUNEL positive cells remained significantly greater than that in NS group (P<0.05).CONCLUSION: The expression of GFAP and NF-κB in the hippocampus increased after SC in rats .Baicalin decreases the expression of GFAP and NF-κB in hippocampus of rats with pilocarpine-induced seizures , and reduces the number of neuronal apoptosis , sug-gesting that baicalin may protect against the brain damage caused by status convulsion .

Objective To study the clinical effect of cervical loop electrosurgical excision procedure (LEEP) in diagnosis and treatment of cervical diseases (including cervical intraepithelial neoplasia of different grade, cervical erosion of moderate and severe degree, cervical with human papillomavims (HPV) infection and cervi-cal polyp) . Method The data of 136 cases with cervical disease treated by LEEP were analyzed retrospectively. Re-sult The postoperative diagnosis were upgraded in 18 cases (13.2%) ,and downgraded in 38cases (27.9 %) . The preoperative diagnosis of 80 cases (58.8 %) were in accordance with the postoperative ones. The difference was sig-nificant(P<0.05). The mean operative time was 11minutes, the mean bleeding volume was 15 ml. Postoperative bleeding of LEEP occurred in 2.2 % (3/136) patients. Postoperative cervical conglutination of LEEP occurred in 1. 5 % (2/136) patients. The cure rate of CIN was 96 % after LEEP. The residual CIN occurred in 4 patient (4 %). Recurrence CIN occurred in 2 patient (2 %). At 12th months after LEEP,the negative rate of HPV detection was 86. 7% and the persistent positive rate was 13.3%. The HPV clearance rate was increased gradually after LEEP. Conclu-sion LEEP was safe and effective for the treatment of the cervical disease and was a kind of good method to clear HPV infection in cervical.

Objective To explore the clinical value of the serum cystatin C (Cys C) changes in different periods of the infantile pneumonia.Methods Ninety-two cases of infantile pneumonia (59 mild pneumonia patients and 33 severe pneumonia patients) from November 2012 to March 2013 were collected and 40 cases of healthy infant were enrolled for control group.The levels of serum Cys C,urea and creatinine in acute and recovery period were detected.Results In severe pneumonia patients,the average level of Cys C in acute period [(1.98±0.33) mg/L] is significantly higher than that in control group [(0.85 ±0.24) mg/L] (P ＜0.01),and there was no significant difference between recovery period [(1.12 ± 0.23) mg/L] and control group (P ＞ 0.05),and there was significant difference between acute and recovery period (P ＜0.05).In mild pneumonia patients,there were no significant differences in the level of Cys C between the acute period [(1.10 ±0.22) mg/L] and recovery period [(0.94 ±0.21) mg/L] as well as control group (P ＞ 0.05).The positive rate of Cys C in severe pneumonia patients (51.9％,14/27) was higher than that of urea and creatinine (3.7％,1/27) (P ＜0.01).Conclusion Severe pneumonia could result in renal dysfunction,which is reversible.Cys C can be the clinical reference for early diagnosis and the therapeutic effect in severe pneumonia patients with renal dysfunction.

Objective To provide an essential features of the knee for the prothesis design more suitable in Chinese. Methods 240 knees of 120 healthy Chinese adults were selected on trial, with an average age of 30.6 years (range, 21 to 40 years) and an average height of 1.68 m ranging from 1.60 m to 1.75 m. Each individual was asked to do deep flexion activities. Fluoroscopy were used to determine accurately the weightbearing kinematics in vivo of 240 knees at different position. The anterior articular surface of the femoral condyle was designated as the "extension facet"(EF), while the posterior surface as the "flexion facet"(FF). Their centers are labelled as EFC and FFC respectively. The distances between the ipsilateral posterior tibial cortex and the center of the posterior femoral condylar circles at different position were measured by drawing a line through the most protruding points in the medial and lateral tibial subchondral bony landmark. A second line was drawn perpendicular to the first and tangent to the posterior tibial cortex. The distance d1 and d2 were measured perpendicularly from FFC and EFC to the second line respectively. The change of d1 and d2 in different positions denoted the relative movement between the tibia and femur. The data were used by ANOVA analysis containing two factors with SAS software. Results The difference of sex and side had no influence on the relative movements between the tibia and femur. In normal adults, the range of relative motion at the medial and lateral condyle during deep flexion was (1.4?0.3) mm and (15.9?4.7) mm respectively. Conclusion The lateral condyle undergoes posterior translation including sliding and rolling significantly during the deep flexion that leads to the relative axial rotation of the tibia to the femur.