Objective To examine the distribution and antibiotic resistance of the Acinetobacter baumannii strains isolated from Department of Neurology.Methods A retrospective review was conducted for the 269 strains of A.baumannii isolated during the period from January 2009 to March 2014.VITEK 2 Compact system was used for bacteria identification and antimicrobial susceptibility testing.The data were analyzed with WHONET 5.6 software and compared by Chi-square test.Results The prevalence of A.baumannii was higher in Department of Neurology than in Department of Neurosurgery or Neurology ICU. Majority (97.4%)of the A.baumannii strains were isolated from sputum.About 54.3% of the A.baumannii isolates were resistant to imipenem.Relatively lower percentage of the A.baumannii isolates were resistant to amikacin (20.7%),but as high as 54.3% and 55.0% of the strains were resistant to imipenem and piperacillin-tazobactam.More imipenem-non-resistant A.baumannii strains (89.4%)were resistant to trimethoprim-sulfamethoxazole than imipenem-resistant A.baumannii (72.6%)(P < 0.05). However,to the other antimicrobial agents tested,imipenem-resistant A.baumannii strains showed significantly higher resistance rate than imipenem-non-resistant A.baumannii strains (P< 0.05).Conclusions The prevalence of A.baumannii and imipenem-resistant A.baumannii is high in Department of Neurology.Further monitoring of antibiotic resistance and rational use of antimicrobial agents are helpful to effectively control the epidemic of multidrug-resistant A.baumannii.

Objective To explore the levels of?-radiation emitted from ceramic floor boards saled in Tangshan.Methods The levels of?-radiation emitted from common ceramic floor boards,polished surface-ceramic floor board s and granular surfac-ceramic floor boards manufactured in A,B and C different provinces were determined.All sam ple s were collected from the build ing ma te rial markets in Tangshan.Results The levels of?-radiation emitted from ceram-ic floor boards were91.1,76.8,75.1nGy /h for those manufactured in A,B and C province respectively,and92.7,88.9,78.0nGy /h for granular sur face-ceramic floor boards,polished surface-ceramic floor boards and com mon ceramic floor boards respec tively.Con clu sion For the consumers,the first selection of the common ceramic floor boards to dec-orate the floor was recommended.

ObjectiveTo determine the bispectral index and dose of propofol at which 50 % of patients fall into unconsciousness during induction (BIS50 and EDs0 ). MethodsThirty unpremedicated ASA Ⅰ-Ⅱ patients scheduled for elective surgery were included in this study. The patients did not take any sedatives or hypnotics and had no psychiatric disease. Loss of consciousness and changes in BIS during induction with propofol were studied by sequential experimental method. The loss of responses to verbal command and eyelash stimulation was used as an induction of unconsciousness. The data obtained were analyzed by linear regression and calculated to determine BIS50 and ED50 using weighted averaging method. ResultsThe BIS50 and ED50 for propofol were 79.17 and 1.0192 ng/kg with the 95％ confidence intervals of 72.08-88.55 and 0.94-1.148 mg/kg respectively. Conclusions The sequential experimental method for determination of ED50 is simple, efficient and reliable and also suitable for determination of BIS50.

Mitochondrial quality control is the important mecha-nism that regulates the morphology,quantity and quality of mito-chondrial in cell to maintain cellular homeostasis and thus,cell survival and health.It has been revealed that members of Bcl-2 family are linked to mitochondrial function and integrity.Bcl-2 family proteins are the key regulators of mitochondrial quality control,participating in the signaling pathways regulating the
crosstalk between mitophagy and apoptosis,as well as mitochon-drial fission and fusion.This paper mainly reviews their impact on mitochondrial quality and the major signaling pathways regula-ted by Bcl-2 family proteins.

Objective To determine the fecal elastase-1 (FE-1) in critically ill children in order to investigate the relationships between FE-1 and trypsin,sepsis as well as the severity of the disease.Methods Totally 402 critically ill children admitted in pediatric intensive care unit (PICU) of Hunan Children' s Hospital from July 2013 to March 2014 were studied.The formed stool of patients was collected during the first 24 h after admission.Subjects were divided to 3 groups according to FE-1 concentration:＞ 200 μg/g for normal pancreatic exocrine function (group A,n =300),100-200 μg/g for mild to moderate exocrine pancreatic insufficiency (group B,n =52),＜ 100 μg/g for severe pancreatic exocrine insufficiency (group C,n =50).The analyses of the relationships between FE-1 and pancreatic enzymes,sepsis severity,shock,the number of organ dysfunction,PCIS (pediatric critically ill score),SOFA score,and APACHE Ⅱ score were carried out.Chi-squared test was used for data statistics.The median and four percentile interval were used for the measurement data of abnormal distribution or non-neat variance,the rank sum test of each two of multiple samples compared each other was used for non-parametric test,only when it was statistically significant,and the Spearman method of correlation analysis was used for correlation analysis.Results (1) There was significant difference in serum lipase between group A and group B (P ＜ 0.01).(2) There was statistical difference in FE-1 level between sepsis group and non-sepsis group (P ＜ 0.05).Children with sepsis were divided into three groups according to the severity of sepsis:mild sepsis group,severe sepsis group and septic shock group.There were significant difference in FE-1 level among different severities of sepsis groups and as well as non-sepsis group (P ＜ 0.01).(3) The proportions of FE-1 in septic children of A,B and C groups in comparison with those in non-septic children of three groups were 65.79％ vs.78.13％,15.79％ vs.11.80％,18.42％ vs.10.07％,respectively.The proportions of FE-1 in septic children of B and C groups escalated were higher than those in children without sepsis.(4) The general trend in FE-1 concentrations varied along with the severity of sepsis.There were no significant differences in FE-1 concentration between non-sepsis group and mild sepsis group,and between severe sepsis group and septic shock group,but other paired comparisons between the four groups had statistical significant (P ＜0.01).(5) Along with FE-1 level decreased,the number of organ dysfunction,SOFA score,APS score (This is a part of APACHE Ⅱ score and other part,CPS,is excluded) increased and PCIS score decreased (rs1 =-0.194,P =0.000; rs2 =-0.348,P =0.000; rs3 =-0.176,P =0.000; rs4 =0.185,P =0.000).Conclusions Pancreatic exocrine function damage is associated with sepsis,the pancreatic dysfunction in patients with mild sepsis may not be significant,but its incidence increases gradually with the development of sepsis or with the deterioration of the disease.

Objective To evaluate the practical value of 99m Tc-MIBI scintimammography and breast X-ray in the detection of breast cancer. Methods 100 female patients with 106 suspected focuses of breast cancer on clinical examination underwent 99m Tc-MIBI scintimammography and breast molybdenum target X-ray, and the results were compared with the pathological diagnosis. Results Compared with the histopathologic diagnosis , the sensitivity of molybdenum target X-ray for detecting primary breast cancer was 44.6% and the positive predictability was 31.7%. The specificity of 99m Tc-MIBI was 87.8% and the positive predictability was 76.9%. Conclusions In comparison with breast X-ray, 99m Tc-MIBI scintimammography is of greater clinical value in the diagnosis of breast cancer.

Objective To investigate the drug resistance of patients samples in terms of pathogenic bacteria in order to provide the basis for clinical diagnosis,treatment of blood infection.Methods Six hundred and seventy-one bacteria strains out of 5042 blood samples of hospitalized patients were used to analyze its characters and drug resistance from January 2009 to December 2012 in the people's hospital of inner mongolia autonomous region.BacT AIERT 3D automatic rapid microbial detection system was applied to perform blood culture.The bacteria identification and drug sensitivity test (MIC method) were detected by using VITEK 2Compact automatic bacteria identification instrument.Results Bacteria positive rate was 13.3％ (671/5042),of which the gram negative bacilli accounted for 49.9％ (335/671),and gram positive for 40.8％ (274/671).The top 5 bacteria strains of blood samples were escherichia coli,staphylococcus,staphylococcus aureus,klebsiella pneumoniae and staphylococcus aureus.The pathogenic bacteria rates of blood samples were 30.5％(29/95),44.4％ (55/124) and 52.5％ (94/179) respectively during 2009-2012.The main source of blood bacteria renal were department of internal medicine ward (12.1％,81/671),department of general surgery (11.6％,78/671),and ICU ward (10.6％,71/671).The detected bacteria rate in department of general surgery separation rate increased to the first in 2012 from fifth in 2009.However the detected bacteria rate in department of internal medicine was down to the tenth in 2012 from the third in 2009.The drug resistance rate of imipenem,piperacillin/tazobactam,cefotaxime,ceftazidime resistant cefotetan on escherichia coli and klebsiella pneumoniae were all less than 9.7％,and the rate of linezolid,vancomycin,teicoplanin,quinupristin/dafoe leptin and nitrofurantoin resistance of staphylococcus aureus,staphylococcus bacteria and gold staphylococcus aureus were all less than 2.3％.Conclusion The distribution,sources and drug resistance of pathogenic bacteria had been changed recently.Therefore the laboratory shall strengthen the monitoring of drug resistance of bacteria in the bloodstream infection in order to guide clinical rational application of antibiotics.

Objective To explore the mediating effect of cognitive emotion regulation strategies between emotional intelligence and loneliness. Methods 542 university students were surveyed by the Chinese version of Cognitive Emotion Regulation Questionnaire ( CERQ) ,the Emotional Intelligence Questionnaire and UCLA Loneli?ness Scale,and the correlation analysis was conducted by using SPSS17.0. The path model was set up and its path analysis was conducted by using AMOS17. 0. Results Positive cognitive emotion regulation strategies ( 28. 64 ± 5.90) were positively correlated with emotional intelligence(2.38±0.27, r=0.346, P<0.05) and negatively correla?ted with loneliness(42.29±7.88, r=0.301, P<0.05);Path analysis showed that the estimates of indirect and direct effects support the hypothesis respectively(P<0.05). Conclusion Positive cognitive emotion regulation strategies serve as partial mediators between emotional intelligence and loneliness.

The Hospital Survey on Patient Safety Culture (HSOPSC) questionnaire was used for appraisal in a newly-built general hospital for all the nursing staff, and they were provided with a six-month safety culture training according to appraisal results.The training has elevated the positive response rate of the nursing staff towards all dimensions of patient safety culture, proving that scientific and reasonable safety culture training is conducive to nurses' perception of patient safety culture.