Hypertrophic cardiomyopathy is regarded as an inherited cardiac disorder caused by autosomal dominant mutations.It has the remarkable heterogeneity of hereditary capacity,clinical phenotype,clinical course and prognosis,thus the diagnosis and treatment of this disease is challenging.Assistant examinations,such as Doppler tissue imaging,quantitative tissue velocity imaging,tissue strain imaging,cardiac magnetic resonance and late gadolinium enhancement,are important to early diagnose,guide management and judge prognosis for hypertrophic cardiomyopathy.This paper reviews the progresses of assistant examinations in hypertrophic cardiomyopathy.

Objective:To study the association between mutations in the first and second exons of(Succtnate dehydrogenaes complex,subunit D(SDHD)gene and sporadic pheochromocytoma in Chongqing Methods:Polymerase chain reaction combining with single strand conformation polymorphism(PCR-SSCP)was selected to analysis the mutations in the first and second exons of SDHD gene in 32 cases of sporadic pheochromocytoma who were diagnosed by pathology(including 21 cases of pheochromocytoma and 11 cases of extra-adrenal paraganglioma)and 80 cases of the healthy controls.Results:No mutation was found in the first and second exons of SDHD gene in the 32 patients.Conclusion:In our study,we had not found any mutation in the first and second exons of SDHD gene in sporadic pheochromo-cytoma in Chongqing.

The paper reports the effect of drugs on the Fc receptor(FcR)and C3b receptor (C3bR)of mouse peritoneal macrophages which was investigaed using in vitro EA-rosette and Yc-rosette techniques,for a further study on mechanism of anti-infection effect of Sheng Ji Ointment.The result of experment has showed that EA-rosette and Yc-rosetteforming rates of Sheng Ji Ointmen activated peritoneal macrophages were higher thanthe controls (p

BACKGROUND: Induced pluripotent stem cells are obtained from somatic cells by reprogramming method. The safety of induced pluripotent stem cells has attracted much attention because of their huge and potential value in clinical application. OBJECTIVE: To review the current studies addressing the safety and clinical application of induced pluripotent stem cells. METHODS: The PubMed database between 2006 and 2012 was retrieved by the first author to search the correlative documents concerning the safety and clinical application of induced pluripotent stem cells. Total y 203 papers were primarily gotten. Final y, 47 papers were included. RESULTS AND CONCLUSION: At present, the main methods to enhance the safety of induced pluripotent stem cells include avoiding usage of c-Myc gene, another mediate way replacing the retrovirus, direct leading of reprogramming factor protein, safer donor cells, micromolecule compound and other in-transgenosis ways. Induced pluripotent stem cells have extensive clinic treatment prospects, and can be used for the build of disease-specific induced pluripotent stem cells line.

BACKGROUND: Oyster shell has been recently reported as a new root canal filling material; therefore, it needs primary biological evaluation, i.e., cytotoxicity test and genotoxicity test, before clinical application. OBJECTIVE: To evaluate cytotoxicity and genotoxicity of oyster shell powder. DESIGN, TIME AND SETTING: A contrast observational study was performed at the Materials Laboratory, School of Stomatology of Peking University from April to July 2008. MATERIALS: Oyster shell powder was provided by Institute of Oceanology, Chinese Academy of Sciences; L929 cell strain and Salmonella typhimurium TA97, TA98, TA100, and TA102 were provided by the National Institute for the Control of Pharmaceutical and Biological Products. METHODS: Oyster shell diffusion was prepared based on the times of 0.5, 1, and 2. According to the standards of biological evaluation of dental materials, the cytotoxity and genotoxicity of oyster shell were evaluated with molecule filter test and Ames test. MAIN OUTCOME MEASURES: Fading area of the film induced by succinic dehydrogenase and amount of spontaneous revertants. RESULTS: After 2 hours, the results indicated that fading area of the film in the experimental group was the same as negative control, i.e., fading was not found in the film, and cytotoxicity was grade 0. After 24 hours, diameter of non-stained film was 1.0-2.0 mm, fading area was 1-3 mm2, and cytotoxicity was grade 1, suggesting that the material had light cytotoxity that was still qualified. The spontaneous revertant test indicated that the mutagenicity of experimental groups was no more than 2 times of the control groups, suggesting there was no dose-dependence. CONCLUSION: Oyster shell powder has no cytotoxicity and genotoxicity on the organism.

As a special branches of capillary electrophoresis(CE), nonaqueous capillary electrophoresis (NACE) was variedly applied in the recent years. A brief summary on solvent properties and molecular interactions in nonaqueous solutions are introduced. Discussing of the approaches employed to enhance and tune selectivity in NACE. Special detection techniques for NACE are presented. Furthermore, a list of relevant applications in biological sample and its metabolin and also in pharmaceutical since 2000 is included, and also take perspective for NACE.

Objective Summarize the nursing experiences of abdominoscope for patients with ovarian cysts. Methods Enough preoperative evaluation, careful perioperative nursing and observation were used in 150 patietns with ovarian cysts when cured by abdominoscope. Results All the patients had accepted succesful operation, there were no intensive complication among the patients. Conclusions Proper nursing measures should be developed with therapeutic laparoscopy, nursing cares according to the operation can effetive shorten the course of rehabilitation for patients.

Two hundred and thirty two patients with essential hypertension were divided into intervention and control groups (116 in each) ,by the end of study period (from January 2005 to January 2006) 106 patients in intervention group (91.4%) and 102 in control group (87.9%) completed observation.The main findings were as follows: the blood pressure control rate in intervention group was higher than that in controls (X2=23.92,P <0.01) ;the family adaptability(X2 =31.12,P <0.01),family caring(X2=37.90 ,P<0.01),family communication (X2= 17.91,P<0.01) and family function(X2 = 8.26,P<0.01)in intervention group were better improved than those in control group.The results suggest that family can be effective resources for improvement of patients with essential hypertension.

Objective To detect the expression of vascular endothelial growth factor(VEGF)-C and VEGF-D in normal gastric tissue and gastric carcinoma tissue,and further investigate the relationship between VEGF-C,VEGF-D and lymph vessel density (LVD),lymph node metastasis.Methods The expression of VEGF-C and VEGF-D in gastric carcinoma tissues were examined by using SP immunohistochemical method in 30 normal gastric tissue and 75 gastric carcinoma tissue respectively.The lymph vessel were marked by LYVE-1 and calculated LVD.Results The rate of positive expression of VEGF-C and VEGF-D in gastric carcinoma tissue was significantly higher than that in normal gastric tissue,there was significant difference [73.3 ％ (55/75) vs.33.3 ％ (10/30),66.7 ％ (50/75) vs.30.0 ％ (9/30),P ＜0.01].The level of LVD in gastric carcinoma tissue with lymph node metastasis (40 cases) was significantly higher than that in without lymph node metastasis (35 cases)[(8.85 ± 1.24)/HP vs.(4.75 ± 1.04)/HP](P =0.000).The level of LVD increased when the expression level of VEGF-C and VEGF-D gradually went up.Conclusions The level of LVD increases in gastric carcinoma tissue.VEGF-C and VEGF-D are two significant factors for LVD and lymph node metastasis.

Cell transplantation has great potentials in repairing damaged tissue.If we want to use the cell transplants to treat diseases that respond poorly to the conventional treatment,or provide better treatments,in vivo dynamic tracking is particularly important.The application of superparamagnetic iron oxide (SPIO) improves a common inconvenience of the current invasive tests including immunohistochemical study or transmission electron microscopy (TEM) study and so on.Researchers has established a variety of preparation methods of the particles,groped the optimal condition of cell marking.SPIO were proved to be feasible and superior in cell tracking in vivo through animal experiments.The results provide the reliable means of using SPIO to track cell in clinical treatments.This review gives a summary of the related study.