Objective:To study the association between mutations in the first and second exons of(Succtnate dehydrogenaes complex,subunit D(SDHD)gene and sporadic pheochromocytoma in Chongqing Methods:Polymerase chain reaction combining with single strand conformation polymorphism(PCR-SSCP)was selected to analysis the mutations in the first and second exons of SDHD gene in 32 cases of sporadic pheochromocytoma who were diagnosed by pathology(including 21 cases of pheochromocytoma and 11 cases of extra-adrenal paraganglioma)and 80 cases of the healthy controls.Results:No mutation was found in the first and second exons of SDHD gene in the 32 patients.Conclusion:In our study,we had not found any mutation in the first and second exons of SDHD gene in sporadic pheochromo-cytoma in Chongqing.

Hypertrophic cardiomyopathy is regarded as an inherited cardiac disorder caused by autosomal dominant mutations.It has the remarkable heterogeneity of hereditary capacity,clinical phenotype,clinical course and prognosis,thus the diagnosis and treatment of this disease is challenging.Assistant examinations,such as Doppler tissue imaging,quantitative tissue velocity imaging,tissue strain imaging,cardiac magnetic resonance and late gadolinium enhancement,are important to early diagnose,guide management and judge prognosis for hypertrophic cardiomyopathy.This paper reviews the progresses of assistant examinations in hypertrophic cardiomyopathy.

The paper reports the effect of drugs on the Fc receptor(FcR)and C3b receptor (C3bR)of mouse peritoneal macrophages which was investigaed using in vitro EA-rosette and Yc-rosette techniques,for a further study on mechanism of anti-infection effect of Sheng Ji Ointment.The result of experment has showed that EA-rosette and Yc-rosetteforming rates of Sheng Ji Ointmen activated peritoneal macrophages were higher thanthe controls (p

BACKGROUND: Oyster shell has been recently reported as a new root canal filling material; therefore, it needs primary biological evaluation, i.e., cytotoxicity test and genotoxicity test, before clinical application. OBJECTIVE: To evaluate cytotoxicity and genotoxicity of oyster shell powder. DESIGN, TIME AND SETTING: A contrast observational study was performed at the Materials Laboratory, School of Stomatology of Peking University from April to July 2008. MATERIALS: Oyster shell powder was provided by Institute of Oceanology, Chinese Academy of Sciences; L929 cell strain and Salmonella typhimurium TA97, TA98, TA100, and TA102 were provided by the National Institute for the Control of Pharmaceutical and Biological Products. METHODS: Oyster shell diffusion was prepared based on the times of 0.5, 1, and 2. According to the standards of biological evaluation of dental materials, the cytotoxity and genotoxicity of oyster shell were evaluated with molecule filter test and Ames test. MAIN OUTCOME MEASURES: Fading area of the film induced by succinic dehydrogenase and amount of spontaneous revertants. RESULTS: After 2 hours, the results indicated that fading area of the film in the experimental group was the same as negative control, i.e., fading was not found in the film, and cytotoxicity was grade 0. After 24 hours, diameter of non-stained film was 1.0-2.0 mm, fading area was 1-3 mm2, and cytotoxicity was grade 1, suggesting that the material had light cytotoxity that was still qualified. The spontaneous revertant test indicated that the mutagenicity of experimental groups was no more than 2 times of the control groups, suggesting there was no dose-dependence. CONCLUSION: Oyster shell powder has no cytotoxicity and genotoxicity on the organism.

BACKGROUND: Induced pluripotent stem cells are obtained from somatic cells by reprogramming method. The safety of induced pluripotent stem cells has attracted much attention because of their huge and potential value in clinical application. OBJECTIVE: To review the current studies addressing the safety and clinical application of induced pluripotent stem cells. METHODS: The PubMed database between 2006 and 2012 was retrieved by the first author to search the correlative documents concerning the safety and clinical application of induced pluripotent stem cells. Total y 203 papers were primarily gotten. Final y, 47 papers were included. RESULTS AND CONCLUSION: At present, the main methods to enhance the safety of induced pluripotent stem cells include avoiding usage of c-Myc gene, another mediate way replacing the retrovirus, direct leading of reprogramming factor protein, safer donor cells, micromolecule compound and other in-transgenosis ways. Induced pluripotent stem cells have extensive clinic treatment prospects, and can be used for the build of disease-specific induced pluripotent stem cells line.

As a special branches of capillary electrophoresis(CE), nonaqueous capillary electrophoresis (NACE) was variedly applied in the recent years. A brief summary on solvent properties and molecular interactions in nonaqueous solutions are introduced. Discussing of the approaches employed to enhance and tune selectivity in NACE. Special detection techniques for NACE are presented. Furthermore, a list of relevant applications in biological sample and its metabolin and also in pharmaceutical since 2000 is included, and also take perspective for NACE.

Objective Summarize the nursing experiences of abdominoscope for patients with ovarian cysts. Methods Enough preoperative evaluation, careful perioperative nursing and observation were used in 150 patietns with ovarian cysts when cured by abdominoscope. Results All the patients had accepted succesful operation, there were no intensive complication among the patients. Conclusions Proper nursing measures should be developed with therapeutic laparoscopy, nursing cares according to the operation can effetive shorten the course of rehabilitation for patients.

Objective To evaluate the variability of four parameters of multi-focal electroretinogram (mERG) a-wave amplitude, b-wave amplitude, a-wave latent period, b-wave latent period. Methods Sixty normal eyes of 46 volunteers were divided into 3 groups of different ages. RETIscan 3 12 system was used to carry out mERG examination. The stimulus matrix of 61 hexagonal elements spanning the central 24?of the visual field. These hexons were scaled with eccentricity and divided into 5 rings. First-order kernel was selected. Results The variability of four parameters of mERG was great. The variability of b-wave latent period was the smallest, its coefficient of variatian was 4.52%～15.62%;that of a-wave latent period held the second place:10.29%~48.67%;that of b-wave amplitude was greater:25.92%~76.11%;that of a-wave amplitude was the greatest:43.82%~88.23%. The results of three groups showed that b-wave amplitude of ring 1 had the smallest variability. Conclusions The variability of latent period is smaller than that of amplitude; the variability of b-wave was smaller than that of a-wave. The longer the centrifugal distance, the lower the amplitude density of a-wave and b-wave. Physiological and anatomical factors might be important for the variability of parameters of multi-focal electroretinogram.

Objective To construct specifically expressed vascular endothelial growth factor (VEGF) 165 gene in retina. Methods Rho promoter, specifically expressed in retina, was amplified by polymerase chain reaction (PCR) from the genomic DNA of a BLAB/C rat, then it was cut with restriction enzymes and cloned into the plasmid pcDNA 3.1+-VEGF 165 to form recombinant plasmid pcDNA 3.1+-rho-VEGF 165. The correct recombinant plasmid pcDNA 3.1+-rho-VEGF 165 was identified by restriction enzymes and PCR, and was transferred by jetPEI into cultured human navel vein endothelial cells and human retinal pigment epithelial (RPE) cells. The expression of VEGF protein in human navel vein endothelial and RPE cells was detected by immunocytochemical staining and protraction of the growth curve of the cells. Results In human RPE cells, the expression of VEGF protein was more in recombinant plasmid pcDNA 3.1+-rho-VEGF 165 than that in plasmid pcDNA 3.1+-VEGF 165; in human navel vein endothelial cells, no obvious difference of the expression of VEGF protein between recombinant plasmid pcDNA 3.1+-rho-VEGF 165 and plasmid pcDNA 3.1+-VEGF 165 was found. Conclusions The construction of pcDNA 3.1+-rho-VEGF 165 carrier may provide the basic material for the study of the nosogenesis of VEGF in retinal neovascularization, and establish the foundation to set up the model of transgenic mice with VEGF specific expressing in retina.

The left ventricular regional systolic functions in patients with hypertrophic cardiomyopathy (HCM) were assessed by using quantitative tissue velocity imaging (QTVI). Left ventricular (LV) regional myocardial velocity along long- and short-axis in 31 HCM patients and 20 healthy subjects were analyzed by QTVI, and the regional myocardial systolic peak velocities (MVS) were measured. Mean MVS at each level including mitral annular, basal, middle and apical segments were calculated. The ratio of MVS along long-axis to that along short-axis (Ri) at basal and middle segments of the LV posterior wall and ventricular septum were calculated. The results showed that mean MVS was slower at each level including mitral annular, basal, middle and apical segments in the HCM patients than that in the healthy subjects (P < 0.01). There were no significant differences in mean MVS between obstructive and non-obstructive groups in HCM patients. MVS of all regional myocardial segments along long-axis in the HCM patients were significantly slower than that in the healthy subjects (P < 0.05), but there was no significant difference in MVS of all regional myocardial segments along long-axis between hypertrophied and non-hypertrophied group in the HCM patients. Ri was significantly lower in the HCM patients than that in the healthy subjects. The LV regional myocardial contractility along long-axis was impaired not only in the hypertrophied wall but also in the non-hypertrophied one in patients with HCM, suggesting that QTVI can assess accurately LV regional systolic function in patient with HCM and provides a novel means for an early diagnosis before and independent of hypertrophy.
Cardiomyopathy, Hypertrophic/*physiopathology ; Cardiomyopathy, Hypertrophic/ultrasonography ; *Echocardiography, Doppler/methods ; Ventricular Function, Left/*physiology