Objective To study the chemical constituents of marine sponge Craniella australiensis collected from the South China Sea.Methods Compounds were isolated and purified by column chromatography and their structures were identified based on spectral analyses and chemical evidence.Results Four compounds were isolated from the ethanol extract and identified as: ?-sitosterol(Ⅰ),3?,6?-stigmast-4-en-3,6-diol(Ⅱ),1,12-diazacyclodocosane-2,11-dione(Ⅲ),uracil(Ⅳ).Conclusion All these compounds were isolated from this sponge for the first time.

OBJECTIVEThe characteristics of lip-mouth region including the soft and hard tissues in smiling position with frontal fixed position photographic computer-aided analysis were studied.

METHODSThe subjects were 80 persons (40 male and 40 females, age range: 17 to approximately 25 years) with acceptable faces and individual normal occlusions. The subjects were asked to take maximum smiling position to accept photographic measurement with computer-aided analysis.

RESULTSThe maximum smile line could be divided into 3 categories: low smile line (16.25%), average smile line (68.75%), and high smile line (15%).

CONCLUSIONThe method adopting maximum smiling position to study the lip-month region is reproducible and comparable. This study would be helpful to provide a quantitative reference for clinical investigation, diagnosis, treatment and efficacy appraisal.

Adolescent ; Adult ; Anthropometry ; Dental Occlusion ; Female ; Humans ; Lip ; anatomy & histology ; Male ; Mouth ; anatomy & histology ; Smiling ; Tooth ; anatomy & histology