Objective To summarize the experience of nursing pregnant women with hyperthyreosis. Methods A retrospective analysis of the nursing histories of 37 pregnant women with hyperthyreosis was done to summarize the nursing interventions of pregnancy-induced hypertension syndrome,heart failure and hyperthyroidism crisis. Results Among all the patients,6 cases developed with pregnancy-induced hypertension syndrome,4 with heart failure and 2 with hyperthyroidism crisis.They were cured and discharged due to treatments and nursing intervention. Conclusion Enhanced monitoring and nursing of perinatal complications is critical for pregnancy outcome of pregnant women with hyperthyreosis.

Objective: To evaluate the outcome of modified rotary-propulsion facial artery perforator flaps for infraorbital defects repair, after facial tumorresection. Methods: Between January 2014 and June 2017, 21 patients with midface tumor were treated, including basal cell carcinoma (n=17) and squamous cell carcinoma (n=4). The size of the tumor ranged from 0.8 cm × 2.0 cm to 2.0 cm× 3.5 cm. The extended resection of the tumor tissue was performed, based on the tumor type. Intraoperative frozen specimen was used to make sure no tumor residual at margin or wound base. According to the location and size, anarterial perforator flap was designed to cover the defect.The donor site was closeddirectly. The flap size ranged from 1.5 cm × 3.0 cm to 3.0 cm × 5.0 cm. The length of flap pedicle was 1.0 cm to 2.5 cm. Results: All flaps completely survived, with satisfying blood flow.The incisions healed well. After 12 to 36 months follow-up, most scars in the donor site were hidden in nasal buccal sulcus and nasolabial folds. There was no lower eyelid ectropion, eyelid and eyeball separation, oral commissural and nasal distortion. The texture and color of the flap was similar to surrounding skin.The overall appearance is satisfactory. Conclusions The modified rotary-propulsion facial artery perforator flaps, to repair soft tissue defects in infraorbital area, is reasonable and useful. It is characterized by limited tissue damage indonor site, flexible rotation of flaps, and hidden incision scars. More importantly, it can reduce the occurrence of lower eyelid ectropion

Objective To investigate the effect of local injection of rat adipose-derived mesenchymal stem cells (ADSCs)on the phenotype of macrophages in skin wound.Methods The cryopreserved primary SD rat ADSCs were resuscitated and then sub-cultured.ADSCs of the third generation were used in the experiments.Thirty six SD rats were divided into ADSCs group (n =18) and control group (n =18) by random numbers table method.The full thickness skin wounds were established on bothsides of the spine.After the model establishment 0.2 ml ADSCs suspension labeled by live cell stain Chloromethylbenzamido derivatives of 1,l'-dioctadecyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate (CM-Dil) with the concentration of 5 × 106/ml was subcutaneously annularly injected in the skin wound of SD rats in ADSCs group.The SD rats in control group were given 0.2 ml serum-free Dulbecco modified Eagle medium (DMEM).On 3,7,and 14 days after injury,six rats were selected from each group to measure the wound area and healing rate.The healed wound tissues were harvested to observe the morphology by HE staining.The expressions of interleukin (IL)-10 were detected by immunohistochemistry staining.The double-positive expressions of CD68 and inducible nitric oxide synthase (iNOS) (M1 type macrophages) and of CD68 and arginase-1 (Arg-1) (M2 type macrophages) were detected by immunofluorescent staining.The distribution of CM-Dil-labeled ADSCs in healed wound tissue 14 days after injury was observed by inverted fluorescence microscope.Results (1) At day 3 after injury,the wound areas in two groups were covered with crust and surrounding redness,and the wound healing rates were slightly different;at day 7 after injury,the wound area of ADSCs group was significantly smaller than that of control group,and the healing speed and rate of ADSCs group was significantly higher than that of control group (P ＜ 0.01);at day 14 after injury,the healing rate of ADSCs group was nearly 99％ (P ＜ 0.01),and the healing skin tissue texture of ADSCs group was better than that of control group.(2) At day 3 after injury,there were a large number of inflammatory cells and disorganized collagen fiber in the wound areas of two groups;at day 7 after injury,the inflammatory cells infiltration reduced in ADSCs group compared with control group,and the collagen fiber arrangement in control group was in disorder;at day 14 after injury,the inflammatory cells in both groups obviously decreased,and ADSCs group had more new vessels and more orderly arrangement of collagen fiber than control group.CM Dil labeled ADSCs were seen in the healing wound tissue in ADSCs group.(3) At day 3 after injury,there was little difference in M1 type macrophage distribution in the two groups;ADSCs group had more M2 type macrophage cells than control group significantly (P ＜0.01);the expression of IL 10 in ADSCs group was not high,which did not differ from that of control group;at days 7 and 14 after injury,ADSCs group has fewer M1 type macrophage cells,more M2 type macrophage cells,and higher expressions of IL-10 than control group (P ＜ 0.01).Conclusion The ADSCs trasplantation can promote the change from M1 type to M2 type macrophages,facilitating wound regeneration and healing.

Objective To study the different prescriptions of eletcro-acupuncture ( distal-proximal acu-points, proximal acupoints and distal acupoints) on pain-related behaviors and expression of microglia marker OX42 and cyclooxygenase 2 ( COX2 ) in spinal dorsal horn in rat model of transplantation of autol-ogous nucleus pulposus, so as to provide experimental basis for the mechanism of the different prescrip-tions. Methods 54 healthy male SD rats were randomly divided into six groups ( each n=9 )-normal group, model group, sham operation group, distal-proximal acupoint group, proximal acupoint group, distal acupoint group. After modeling, the rats in three acupuncture groups were treated for 20 minutes, once daily for consecutive 7 days. Those in distal-proximal acupoint group were needled at bilateral “L5 Jiajixue” ( Ex-B2 ) ,“Dachangshu” ( BL 25 ) ,“Weizhong” ( BL 40 ) and“Kunlun” ( BL 60 );those in proximal acupoint group were needled at bilateral“L5 Jiajixue” ( Ex-B2 ) and“Dachangshu” ( BL 25 ) ;the rats in distal acupoint group rats were needled at bilateral “Weizhong” ( BL 40 ) and “Kunlun” ( BL 60). Thermal withdrawal latencies were evaluated. The expressions of OX42 and COX2 in the left spinal dorsal horn were detected using immunohistochemical methods. Results Thermal withdrawal latencies and the expression of OX42 and COX2 increased in rat model of transplantation of autologous nucleus pul-posus. After treatment, thermal pain threshold was improved (P<0. 05), and the expression of OX42 and COX2 were inhibited ( P<0 . 05 ) . The inhibitory effects of distal-proximal acupoint group and proxi-mal acupoint group were superior to that in distal acupoint group. Conclusion Each acupuncture pre-scription reduced anti-inflammatory effects by inhibiting inflammatory signaling pathways in spinal dorsal horn, with the prescription of distal-proximal acupoints and proximal acupoints superior to distal acu-points.