Objective To investigate the effect of intermittent pneumatic pressure pump on the prevention of postoperative deep vein thrombosis(DVT)in elderly patients with lung cancer.Methods One hundred and fifteen lung cancer patients were divided into the control group(n=59)and the test group(n=56).The control group received routine nursing care for the prevention of DVT and the test group were managed with intermittent pneumatic pressure pump apart from the routine nursing as in the control one.The two groups were compared in terms of deep phlebothrombosis and edema of lower limbs.Result There were significant differences between the groups in deep phlebothrombosis and edema of lower limbs as well as the incidence of DVT(P<0.05).Conclusion Intermittent pneumatic pressure pump can prevent postoperative deep venous thrombosis effectively.

Objective To investigate the relationship between the level of tumor necrosis factor (TNF)? and the clinical features of multiple sclerosis(MS). Methods The TNF? level of 58 MS patients (MS group) was determined by means of sandwich enzyme-linked immunosorbent assay (ELISA). The correlative analysis between level of TNF? and the Expanded Disability Status Scale (EDSS), the clinical data (including the duration of disease,the number of relapses and the age of onset) were take in MS patients. Results (1)TNF? level was significantly increased in MS acute stage compared with those in MS remission stage and healthy controls (P

Objective To explore the relationship between first-episode schizophrenia and metabolic syndrome (MS). Methods 231 first-episode schizophrenia patients (schizophrenia group) and 63 healthy controls (healthy group) were selected to detect the height, weight, waistline, fasting plasma glucose (FPG), blood lipid and blood pressure. International Diabetes Federation (IDF) MS Diagnostic Criterion (2005) was used to diagnose MS. Results The FPG and Total Cholesterol (TC) of the schizophrenia group were significantly higher than the control group (P<0.001). The MS detection rate in the schizophrenia group was higher than in the control group (P=0.021). Conclusion First-episode schizophrenia patients could have glucose-lipid metabolism disorder during the early stage.

Objective:To investigate the status of glucolipid metabolism and insulin resistance in patients with first-episode non-medicated schizophrenia, and to explore their relationship with psychiatric symptoms and cognitive function.Methods:One hundred and seventeen patients with first-episode non-medicated schizophrenia admitted to Wenzhou Seventh People′s Hospital from January 2018 to August 2020 were included in case group, and 61 healthy subjects with physical examination during the same period were used as control group. The glucose metabolism, including serum fasting blood glucose (FBG), 2 h postprandial blood glucose (2 h PBG), fasting insulin (FINS), fasting C peptide; lipid metabolism, including triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C); apolipoprotein A1 (Apo-A1) and homeostatic model assessment insulin resistance index (HOMA-IR) level were compared between 2 groups. The abnormal glucolipid metabolism and incidence rate of insulin resistance were counted in the two groups. According to the condition of abnormal glucolipid metabolism or insulin resistance in case group, the patients were divided into abnormal glucolipid metabolism group and non-abnormal group, and insulin resistance group and non-insulin resistance group. The psychiatric symptoms (positive and negative symptom scale, PANSS) and cognitive function (MATRICS consensus cognitive battery, MCCB) were compared between 2 groups. Pearson correlation analysis was used to explore the correlation between glucolipid metabolism and insulin resistance and psychiatric symptoms and cognitive function in case group.Results:The levels of glucolipid metabolism indexes of 2 h PBG, FINS, fasting C peptide, TG and HOMA-IR in case group were significantly higher than those in control group: (7.06 ± 1.88) mmol/L vs. (6.19 ± 1.53) mmol/L, (8.61 ± 2.46) mU/L vs. (6.25 ± 1.71) mU/L, (0.49 ± 0.16) nmol/L vs. (0.32 ± 0.09) nmol/L, (1.33 ± 0.47) mmol/L vs. (1.02 ± 0.24) mmol/L, 2.01 ± 0.71 vs. 1.51 ± 0.45 ( P<0.05); while the levels of HDL-C and Apo-A1 were significantly lower than those in control group: (1.19 ± 0.38) mmol/L vs. (1.57 ± 0.32) mmol/L, (1.21 ± 0.25) g/L vs. (1.43 ± 0.17) g/L ( P<0.05). The total incidence rate of abnormal glucolipid metabolism or insulin resistance in case group was significantly higher than that in control group: 62.39%(73/117) vs. 13.11%(8/61) ( P<0.05). The scores of dimensions of positive symptoms, negative symptoms and general psychopathology and total score of PANSS in combined group were significantly higher than those in non-abnormal group: (25.14 ± 5.09) scores vs. (22.95 ± 4.72) scores, (24.68 ± 5.25) scores vs. (22.05 ± 4.59) scores, (41.52 ± 5.85) scores vs. (38.12 ± 4.18) scores, (94.68 ± 11.64) scores vs. (85.43 ± 8.51) scores ( P<0.05). The above scores points in insulin resistance group were higher than points in non-insulin resistance group: (26.62 ± 4.18) scores vs. (23.62 ± 4.98) scores, (25.92 ± 5.07) scores vs. (23.02 ± 4.96) scores, (42.94 ± 5.26) scores vs. (39.43 ± 4.47) scores, (97.35 ± 10.07) scores vs. (89.37 ± 10.25) scores ( P<0.05). The scores of continuous performance test-identical pairs (CPT-IP), working memory (WM), brief visuospatial memory test-revised (BVMT-R) and Mayer-Salovey-Caruso emotional intelligence test (MSCEIT) of MCCB scale in abnormal glucolipid metabolism group were significantly lower than those in non-abnormal group: (23.82 ± 5.21) scores vs. (27.15 ± 4.69) scores, (21.72 ± 5.95) scores vs. (25.35 ± 5.14) scores, (19.56 ± 5.28) scores vs. (22.34 ± 5.43) scores, (22.62 ± 5.13) scores vs. (26.47 ± 4.96) scores ( P<0.05), and the scores in insulin resistance group were significantly lower: (22.26 ± 4.84) scores vs. (25.42 ± 5.12) scores, (20.35 ± 4.87) scores vs. (23.46 ± 5.08) scores, (18.05 ± 4.27) scores vs. (20.98 ± 5.71) scores, (21.15 ± 4.67) scores vs. (24.48 ± 5.02) scores ( P<0.05). Pearson correlation analysis showed that 2 h PBG in case group was positively correlated with PANSS positive symptoms ( P<0.05), and was negatively correlated with CPT-IP and MSCEIT in MCCB scale ( P<0.05). FINS and HOMA-IR were positively correlated with positive symptoms, negative symptoms and PANSS total score ( P<0.05), and were negatively correlated with CPT-IP, WM, BVMT-R and MSCEIT ( P<0.05). HDL-C was negatively correlated with positive symptoms ( P<0.05), and was positively correlated with CPT-IP, WM and MSCEIT ( P<0.05). Apo-A1 was negatively correlated with positive symptoms and negative symptoms ( P<0.05), and was positively correlated with CPT-IP and WM ( P<0.05). Conclusions:Abnormal glucolipid metabolism and insulin resistance have a higher detection rate in first-episode non-medicated schizophrenia, and have a certain relationship with the psychiatric symptoms and cognitive impairment of patients.

0.05).Conclusions The TNF-?levels differed among the sta- ges of disease and MS subtypes,but no marked relation existed between the TNF-?level and the EDSS score.

Purpose To explore the association of NET-1 expression with the angiogenesis of cervical squamous cell carcinoma and the related molecular mechanism.Methods Immunohistochemistry MaxVision was used to detect the expression of NET-1,NF-κB p65 and VEGF in 80 cases of cervical squamous cancer tissue and 10 cases of chronic cervicitis tissue.The microvascular density (MVD) was assessed by labeling endothelia with CD34-antibody and counted according to Weinner's standard.The eukaryotic expression plasmid pCMV6-NET-1 was transfected into cervical squamous cancer cells by cationic liposome in order to obtain over expression of NET-1 gene.In order to inhibit the activation of NF-kappa B,cervical squamous carcinoma cells were treated with NF-kappa B specific inhibitor PDTC.The protein expression in SiHa cells was assessed by Western blotting.Results The expression level of NET-1 in the cervical squamous carcinoma tissue was significantly higher than that in the tissue of control group.NET-1 expression was positively correlated to the MVD,NF-κB p65 and VEGF expression,respectively.Transient transfection of NET-1 gene significantly increased the nuclear expression of NF-κB p65 and cytoplasm expression of VEGF in the SiHa cells.However,pretreatment of SiHa cells with NF-inhibitor PDTC significantly inhibited NET-1 induced upregulation of VEGF expression.Conclusion NET-1 promotes the angiogenesis of cervical squamous carcinoma through the activation of the NF-κB signaling which upregulate the expression of VEGF in the cancer cells.

Objective To study the changes of the T-lymphocyte subsets in patients with Guillain-Barré syndrome(GBS)before and after intravenous immunoglobulin treatment(IVIG),and to explore the possible mechanism of the IVIG curing GBS further.Methods Chose 31 cases of clinically confirmed GBS were enrolled and compared before and after the treatment.According to the effect of the therapy,31 cases of the total were sub-divided into effective and ineffective groups.Relative counting of peripheral blood T-lymphocyte subsets was preformed with flow cytometry.Results ①The percentage of CD8+ T and CD4+CD29+ T cell was significantly lower(CD8+T:28.77%±11.02% vs 31.84%±12.35%,CD4+CD29+T:56.71%±12.44% vs 62.40%±12.72%,t=2.995,3.919,P＜0.05)after therapy,while the rate of CD4+/CD8+T and the percentage of CD4+CD45RA+T cell increased notably(t=2.368,3.860,P＜0.05);but there was no notable difference in the percentage of CD3+T and CD4+T cell.②The percentage of CD8+T and CD4+CD29+ T cell was significantly lower(t=2.144,3.343,P＜0.05)after the treatment,while the rate of CD4+/CD8+T and the percentage of CD4+CD45RA+T cell increased notably(t=2.159,3.277,P＜0.05)in the good curative effect group,but there was no change in the bad curative effect group.③61.29%(19/31)of the patients significantly improved by IVIG,and there was no death case.Conclusions T-lymphocyte subsets change in a varing degree after IVIG treatment in acute GBS patients,which lays an immunological foundation for the further study of pathogenesis and mechanism of IVIG curing GBS;effective on GBS,IVIG can actively suppress pathogenetic condition and promote the recovery of nrevous function.

Soft-tissue deformation is one of the important research directions in virtual surgery. Mass-spring model and finite-element model are the two most important modeling technology of the soft tissue modeling. It is very important for the current soft-tissue deformation modelling to analysize, to summarize the main idea, the influence factors and the modeling route of the two kinds of modeling methods and then to give comprehensive review.
Computer Simulation ; Humans ; Models, Biological ; Surgery, Computer-Assisted ; methods ; User-Computer Interface

Objective:To compare the effects of repetitive transcranial magnetic stimulation (TMS) at three different relatively high frequencies on neuropathic pain so as to find the best frequency.Methods:One hundred Sprague-Dawley rats were randomly divided into a sham operation group, a model group, a TMS group and a control stimulation group. The TMS group was further divided into a 5Hz group, a 10Hz group, and a 20Hz group. The rats in the model, control stimulation and TMS groups received constriction injury of the sciatic nerve, while the rats in the sham group were given a sham version of the operation. On the third day after the operation the rats in the TMS group and the control stimulation group began to receive TMS treatment. Neuropatic pain was evaluated on the day before the operation, and on the 3rd, 5th, 7th, 10th and 12th days after the operation. The evaluations included the paw withdrawal thermal latency (PWTL) and the paw withdrawal mechanical threshold (PWMT).Results:The average PWTLs and PWMTs in all of the TMS groups increased with the TMS treatment. Those of the 10Hz and 20Hz groups were significantly higher than the 5Hz group′s average, while there were no significant differences between the 10Hz group and 20Hz group.Conclusions:High-frequency TMS at different frequencies has different effects on neuropathic pain, at least in rats. The treatment efficacy at 10 and 20Hz is superior to that at 5Hz.

Objective To investigate the expression level of tumor necrosis factor α( TNF-α) , heat shock protein 70 (HSP70) in serum and to analyze the correlation of TNF-αand HSP70 with the incidence of type 2 diabetes mellitus (T2DM) among Hui and Han nationalities in Ningxia .Methods Using the method of case-control study and according to the inclusion and exclusion criteria of case and control groups , 225 patients with T2DM who were treated by Department of Endocrinology and 600 healthy people during health checkups were enrolled in the study between October 2011 and October 2012 .A double antibody sandwich enzyme-linked immunosorbent assay ( ELISA) was used to determine the protein expres-sion levels of TNF-αand HSP70 in serum.Results The expression levels of TNF-αin the total population investigated and Hui population in T2DM group were significantly higher than in the control (t=-3.512,-3.791,all P<0.01).The difference in expression levels of TNF-αbetween the Han people of T 2DM group and control group , and that in the expression levels of HSP70 between the case group and control group were not statistically significant ( t =-0.565,-0.020,-1.251,-0.809,all P>0.05).The elevated serum levels of TNF-αincreased the risk for T2DM(OR=1.500, 95%CI:1.075-2.093,P=0.017).The expression levels of TNF-αin the total people, case and control of Hui people were significant lower than the homologous Han people (t=9.373,2.223,9.734, all P<0.01 or 0.05).Pearson analysis showed that the high expression of TNF-αwas positively correlated with blood glucose level (r=0.110,P=0.001),but there was no correlation between the high expression of HSP 70 and blood glucose level (r=0.023, P=0.504).The result of multiple logistic regression analysis indicated that gender and waist-to-hip ratio(WHR) were risk factors for high levels of TNF-α(P<0.01 or 0.05)while HSP70 and nationalities were protective factors.Systolic blood pressure(SBP) and WHR were risk factors for the high level of HSP70(all P<0.01), but TNF-αwas protective factors.HSP70, TNF-α, gender, family history, BMI, SBP, triglycerides(TG), low density lipoprotein cholesterol (LDL-C), alanine trans-aminase(ALT), and psychological stress were risk factors for T2DM, but high density lipoprotein cholesterol (HDL-C) was a protective fac-tor.Conclusion The high expression of TNF-αin serum is closely related to elevated fasting plasma glucose ( FPG) and T2DM onset.The expression level of TNF-αin serum in Hui people is lower than in Han people ,possibly correlated with ethnic heredity.The high expression of HSP70 in serum may induce T2DM onset.