Objective:To explore the effect of hospital-family CCM on postoperative rehabilitation of children with posterior urethral rupture and its effect on the nursing ability of children′s family members.Methods:Forward-looking selection between January 2015 and January 2019 in our hospital 72 cases of traumatic posterior urethral disruption of children as the research object, the random number table method were randomly divided into 2 groups. The control group ( n=36) was implemented routine nursing, observation group ( n=36) was given hospital-family CCM. The prognosis of postoperative pain and complications of two groups, children′s family psychological status before and after the intervention and nursing ability were compared. Results:Compared with the control group, the scores of Numerical Rating Scale (NRS) of 12h after the operation, 1d after the operation and 2d after the operation respectively 5.89±0.71, 4.50±0.51, 2.42±0.50, all of which were significantly lower than those of the control group 6.92±0.87, 5.89±0.85, 4.31±0.47, with statistically significant differences ( t values were 5.23, 8.904, 16.525, P<0.05). The total incidence of complications in the observation group was 5.56% (2/36), significantly less than 22.22% (8/36) of the control group with statistical significance difference ( χ2 value was 4.181, P<0.05). The scores of Self-rating Anxiety Scale (SAS), Self-rating Depression Scale (SDS), Family Caregiver Task Inventory (FCTI) in the families of children of the observtion group were 34.6±5.36, 31.67±6.11, 8.65±2.40 after the intervention, significantly lower than the control group (41.09±6.44, 39.64±7.31, 11.64±3.10) with statistical difference ( t values were 4.647, 5.019, 4.576, P<0.05). Conclusions:The hospital-family CCM can effectively promote the rehabilitation of children undergoing posterior urethral rupture and improve the nursing ability of their families.

Brain-derived neurotrophic factor (BDNF) exerts its survival-promoting effects on photoreceptors and retinal ganglion cells, however, delivery systems with little-to-no side effect are needed to sustain its controlled release and long-term efficacy. Our previous studies demonstrated that adipose-derived stem cells (ADSCs) are ideal delivery systems for gene therapy; moreover, ADSCs present unique properties like migration to damaged tissue sites, immunomodulation and anti-inflammation. Herein, we propose to employ ADSCs as the BDNF gene delivery vehicle. Different Analyses like flow cytometry, differentiation and cell proliferation assays etc demonstrated that BDNF were successfully transduced into ADSCs and the stemness of ADSCs was maintained even with the transduction. Real Time PCR and Western blot were used to measure mRNA and protein expressions of the BDNF-transduced ADSCs. The results demonstrated that the BDNF expression level of the lentiviral-BDNF transduced ADSCs is significantly increased and, moreover, enhanced the expression of other neurotrophic and downstream signaling factors. The data suggest that ADSCs are a reliable delivery vehicle for BDNF and could be used for the treatment of various diseases.