Objective To investigate the effect of butylphthalide and sodium chloride injection postconditioning on focal cerebral ischemia-reperfusion (I/R) injury and endoplasmic reticulum stress in rats.Methods Thirty-six male SPF Sprague-Dawley rats,aged 2-3 months,weighing 260-280 g,were randomly divided into 3 groups (n =12 each):sham operation group (group S),focal cerebral I/R group (group I/R) and butylphthalide and sodium chloride injection postconditioning group (group Buty).The animals were anesthetized with intraperitoneal 10 ％ chloral hydrate 300 mg/kg.Focal cerebral I/R was induced by occluding right middle cerebral artery for 2 h followed by 24 h reperfusion in I/R and Buty groups.Butylphthalide and sodium chloride injection 2.5 mg/kg was injected via the tail vein immediately after onset of reperfusion in Buty group,while the equal volume of normal saline was injected in I/R group.Neurological deficits were assessed and scored at 24 h of reperfusion,and then the brain was isolated for detection of neuronal apoptosis (by TUNEL) and the expression of glucose-regulated protein 78 (GRP78) and caspase-12 in ischemic cerebral cortex (by immunohistochemistry) in brain tissues.Apoptosis index was calculated.Results Compared with group S,the neurological deficit scores and apoptosis index were significantly increased,and the expression of GRP78 and caspase-12 was up-regulated in I/R and Buty groups (P ＜ 0.05 or 0.01).Compared with group I/R,the neurological deficit scores and apoptosis index were significantly decreased,the expression of GRP78 was up-regulated,and the expression of caspase-12 was down-regulated in group Buty (P ＜ 0.05).Conclusion Butylphthalide and sodium chloride injection postconditioning can reduce focal cerebral I/R injury in rats,and inhibition of endoplasmic reticulum stressmediated cell apoptosis is involved in the mechanism.

Objective This paper aims to describe the prevalence of unhealthy weight control behaviors and its related factors amongst female medical college students. Method Questionnaire surveys were conducted among a total of 798 medical students recruited by cluster random sampling in Jining Medical College from April 5 to 16, 2013. Result A total of 728 valid questionnaires were collected; 29 participants (4.0%) were overweight and 9 participants (1.2%) were obese; 250 participants (34.3%) were losing weight. Among participants who had experience of losing weight, unhealthy weight control methods mainly included not eating staple food (17.3%), drinking diet tea (16.1%), fasting (12.9%), taking diet drug (8.6%), inducing diarrhea (5.4%), inducing vomiting (2.0%), enema (1.5%), etc.; 29.0% (211/728) of participants had unhealthy weight control behaviors. The rates of being weak (c2=19.681, P=0.000), failure to concentrate (c2=12.031, P=0.001), dizziness (c2=5.461, P=0.019), irregular menstruation (c2=24.628, P=0.000), anorexia (c2=32.008, P=0.000), insomnia (c2=11.021, P=0.001) and anxiety (c2=14.402, P=0.000) among participants who had unhealthy weight-control behaviors were higher than those of participants without unhealthy weight?control behaviors. Multinomial logistic regression indicated that the factors associated with unhealthy weight control behaviors were senior class (OR=2.041;95%CI:1.336-3.111), high average family income (OR=1.758;95%CI:1.183-2.611), weight loss intention (OR=6.756;95%CI:1.541-29.626),not hesitating to delay learning (OR=1.594;95%CI:1.078-2.359),insisting on weight loss process regardless of discomfort (OR=2.008;95%CI:1.245-3.238), slimming purpose (body beauty) (OR=1.786;95% CI: 1.095-2.913), and slimming purpose (fashion) (OR=3.169; 95%CI: 1.545-6.498). Subjects with longer dinner time (OR=0.556; 95% CI: 0.341-0.907) and preferring light diet (OR=0.593; 95% CI: 0.406-0.865) had lower rate of unhealthy weight control behaviors. Conclusion Part of female college students are lacking proper understanding of self body shape and unhealthy weight control behaviors and had done harm to their health.

Objective It is to observe the clinical effect of Wenxinkeli combined with metoprolol on frequent ventricular premature beat (FVEB).Methods234 patients with FVEB were randomly divided into the study group and control group.117 patients in the control group were treated with metoprolol combined with placebo, and 117 patients in the stduy group were treated with Wenxinkeli combined with metoprolol.4 weeks were a course of treatment.After four weeks, the heart rate variability, QT interval dispersion and 24h average heart rate of two groups were observed, and the clinical effect were evaluated.ResultsAfter 4 weeks of treatment, 5 cases in the study group and 7 cases in the control group were excluded.The efficiency of control group and study group were 89.29% and 79.09% (P<0.05), respectively.After treatment, the SDNN、SDANN、RMSSD and PNN50 of two groups were significantly higher than those before treatment(P<0.05), and the increase value of the study group was higher than that of the control group(P<0.05).After treatment, the QTd and QTcd of two groups were decreased than those before treatment(P<0.05), and the decreased value of the study group was higher than that of the control group(P<0.05).There was no significant change in the heart rate of the two groups before and after treatment.ConclusionWenxinkeli combined with metoprolol in the treatment of FVEB can prolong HRV, reduce QTd, improve the treatment effect, has no significant effect on the heart rate.

Objective: To understand the iodine nutritional status of residents in the newly identified high iodine and non-high iodine areas in Puyang, and to provide evidence for relevant departments to adjust the iodine supplementation policy. Methods: According to the survey results of drinking water iodine content in 2017, in 2018 stratified and systematic sampling methods were used to extract 25 villages in the newly identified high iodine areas and non-high iodine areas in Puyang City. Each village surveyed 40 children and 20 pregnant women, the urinary iodine content of the subjects and the salt iodine content at their home were measured, and the thyroid volume of children in 10 villages in high iodine and non-high iodine areas was measured. Results: In high iodine areas and non-high iodine areas, the household salt samples collected were 1 357 and 1 544, respectively, the iodized salt coverage rate was 87.91% (1 193/1 357) and 85.62% (1 322/1 544), respectively, and the difference was not statistically significant (χ² = 3.291, P > 0.05). A total of 1 028 children and 329 pregnant women in high iodine areas were investigated, their median urinary iodine content was 337.0 and 289.0 μg/L, respectively; a total of 1 041 children and 503 pregnant women in non-high iodine areas were investigated, their median urinary iodine content were 310.0 and 265.0 μg/L, the differences of the urinary iodine content between high iodine and non-high iodine areas were statistically significant (Z = -3.825, -2.795, P < 0.05). Thyroid volume was measured in 407 children in high iodine areas and 423 children in non-high iodine areas. The thyroid enlargement rate was 8.85% (36/407) and 3.78% (16/423), respectively, and the difference was statistically significant (χ² = 9.053, P < 0.05). Conclusions The iodine nutrition levels of children and pregnant women in high iodine areas are higher than those in non-high iodine areas. Iodized salt should be stopped supplying in high iodine areas as soon as possible. In non-high iodine areas, the feasibility of reducing the iodine concentration of iodized salt should be investigated to ensure the iodine nutrition level of local residents.

Objective: To explore the associations of aggressive behavior with self-esteem with suicide risk in college students. Methods: A total of 4 154 college students in Suzhou, Hefei and Ma’anshan were enrolled in our study by multistage cluster sampling method during October to December 2017. Demographic characteristics, suicide risk, aggression and self-esteem of participants were evaluated by Suicidal Behaviors Questionnaire-Revised (SBQ-R), Buss & Perry Aggression Questionnaire-Chinese Version (AQ-CV) and Rosenberg Self-esteem Questionnaire separately. Aggression and self-esteem were divided into three levels, high (> P 75 ), medium ( P 25 - P 75 ) and low (< P 25 ) level, according to the percentile of the questionnaire score. Multivariate logistic regression model was used to examine the associations of aggression and self-esteem with suicide risk, and to explore gender difference. Results: The gender differences were found in suicide risk, aggressive behavior and self-esteem( χ 2=80.21, 26.47, 26.51 , P <0.01). More girls reported suicide ideation, suicide plan and suicide attempt compared to girls( χ 2=33.03, 25.11, 15.71 , P <0.01). Compared with boys, the detection rate of suicide ideation, suicide plan and suicide attempt of girls were higher( P <0.01). The high aggressive behavior and low self-esteem was correlated with increased risk of suicide, and the interaction effects of aggressive behavior and self-esteem on suicide risk was found. The students with high aggressive behavior and low self-esteem had a higher suicide risk, the multiple adjusted odds ratio was 19.39 (95% CI =11.10-33.87). Gender difference was not found in the associations of aggressive behavior and self-esteem with suicide risk ( P >0.05). Conclusion Aggressive behavior and self-esteem are correlated with suicide risk in college students.

Objective: To investigate the relationship between childhood abuse and recent negative life events with suicidal ideation and attempt, and to provide reference for prevention and control of suicidal behaviors in middle school students. Methods: Stratified cluster sampling method was used to select 7 986 middle school students from Shenzhen, Nanchang and Shenyang as participants. Questionnaire survey was conducted to collect information on demographic indicators, childhood abuse, negative life events, suicidal ideation and suicide attempt, etc. Logistic regression was used to analyze the relationship between childhood abuse experience and negative life events with suicidal ideation and suicide attempt. Results: The prevalence of suicidal ideation and suicide attempt was 22.9% and 4.7%. After adjusted for confounding variables, with the increase of childhood abuse and negative life events, suicidal ideation and attempt in middle school students showed an increasing trend(P<0.05). Compared those with low level of childhood abuse and negative life events, students with high childhood abuse and high negative life events had a higher suicidal ideation and suicide attempt risk, the multiple adjusted odds ratio was 10.43 and 19.75(P<0.05). Conclusion Childhood abuse and negative life events are important risk factors affecting suicidal ideation and attempt in middle school students. Strategies to reduce childhood abuse and negative life events should be an integral component of targeted mental health interventions.

Objective:To investigate the efficacy of a SARS-CoV-2 recombinant protein vaccine as a booster dose.Methods:A new immunogen, namely RBD-sc-trimer, was designed by tandem repeating of single receptor binding domain (RBD) of SARS-CoV-2 spike (S) protein to mimic the trimeric form of RBD presented by the virus. The RBD-sc-trimer protein was expressed as a His-tagged fusion protein using a baculovirus expression system and purified by nickel affinity column. The purified protein was identified by Western blot. Its in vitro binding activity to human angiotensin converting enzyme 2 (hACE2) was analyzed by ELISA. The immunogenicity of RBD-sc-trimer as well as RBD proteins of other forms including RBD dimer (RBD-Fc), RBD monomer (RBD) and S protein trimer (S trimer) as a booster dose was evaluated in BALB/c mice. Results:In terms of both binding and neutralizing antibodies against SARS-CoV-2, RBD-sc-trimer showed an immunogenicity that was superior to that of RBD-Fc and RBD and close to the level of S trimer. The antibody response induced by RBD-sc-trimer was characterized as Th1-biased. Moreover, it displayed a stronger cross-neutralization activity against SARS-CoV-2 Beta, Delta and Omicron variants. The titer of neutralizing antibody against Omicron induced by RBD-sc-trimer only decreased by 9.1 folds relative to the prototype strain, while the antibody response induced by RBD-Fc and S trimer decreased by 68.4 and 70.8 folds, respectively.Conclusions:The recombinant protein, RBD-sc-trimer, which was capable of eliciting stronger humoral response in mice as a booster dose and showed the superiority in raising cross-reactive antibodies against SARS-CoV-2 variants over non-trimeric RBD forms, should be considered as an optimal immunogen for the development of more effective SARS-CoV-2 vaccines.

Objective:To explore the value of ex- vivo porcine stomach model for endoscopic submucosal dissection (ESD) training for international trainees. Methods:Fifteen international students received ESD training and learning for twenty days. Firstly, students learned basic theory of ESD and completed a questionnaire. Then they were randomized to receive endoscopic training either on the vitro animal (group A) or on training experience (group B) of the clinical observation. At last, one case was assessed by an experienced endoscopist. The total and step-by-step operating time, complete resection, size of specimen and complications were recorded. All students completed the questionnaire once again. SPSS 20.0 was used for t test and chi-square test. Results:There was significant difference in total ESD operation time between group A and group B ( P<0.05). There were significant differences between the two groups on cutting time and dissection time, but there was no difference on marking time, injection time and operating speed. There was only one case of block resection and perforation in each group. For group A, their mastery and clinic confidence of ESD had been obviously increased after the animal training course. Compared with the operation before the training in group A, it was found that the total time and cutting time of the ESD after the training were also significantly improved. Conclusion:Theory combined with endoscopic training on vitro animal model can make the trainees familiar with the basic theory and master the operational skills, which is helpful and valuable for them to perform ESD in further clinic practice.

ObjectiveTo establish the specific chromatogram and thin layer chromatography（TLC） of Qingxin Lianziyin（QXLZY） benchmark samples， in order to clarify the key quality attributes and provide a reference for the quality evaluation of QXLZY. MethodHigh performance liquid chromatography（HPLC） specific chromatogram of QXLZY benchmark samples was developed by using a YMC Hydrosphere C₁₈ column（4.6 mm×250 mm， 5 μm） with the mobile phase of acetonitrile（A）-0.2% formic acid aqueous solution（B） for gradient elution（0-10 min， 5%-20%A； 10-20 min， 20%A； 20-25 min， 20%-24%A； 25-40 min， 24%-30%A； 40-55 min， 30%-50%A； 55-65 min， 50%-100%A； 65-75 min， 100%A； 75-75.1 min， 100%-5%A； 75.1-90 min， 5%A）， and the detection wavelength was 360 nm. Ultra-high performance liquid chromatography-linear ion trap/orbitrap mass spectrometry（UHPLC-LTQ-Orbitrap MS） with electrospray ionization（ESI） was used to identify the components of QXLZY benchmark samples by accurate relative molecular weight and multilevel MS fragment ion information， the detection conditions were positive and negative ion modes and data dependency scanning mode. TLC identification methods for Ophiopogonis Radix， Lycii Cortex， Nelumbinis Semen， Poria， Astragali Radix and Ginseng Radix et Rhizoma in QXLZY were established. ResultA total of 15 characteristic peaks were identified from Glycyrrhizae Radix et Rhizoma， Plantaginis Semen and Scutellariae Radix， and the relative standard deviations of the retention times of 15 characteristic peaks in 15 batches of QXLZY benchmark samples were≤3% with peak 8（baicalin） as the reference peak. A total of 100 compounds， including flavonoids， organic acids， saponins， amino acids and others， were identified in the benchmark samples by UHPLC-LTQ-Orbitrap MS. The established TLC had good separation and was suitable for the identification of Ophiopogonis Radix， Lycii Cortex， Nelumbinis Semen， Poria， Astragali Radix and Ginseng Radix et Rhizoma in QXLZY. ConclusionThe material basis of QXLZY benchmark samples is basically determined by MS designation and source attribution. The established specific chromatogram and TLC of QXLZY are simple， stable and reproducible， which can provide a reference for the development and quality control of QXLZY.