Objective To investigate the association between the polymorphisms of apolipoprotein M gene (ApoM) and the risk for cerebral infarction in Han population in northern China.Methods The polymorphisms of ApoM gene were identified by PCR-DNA sequencing analysis and were subsequently detected by PCR-RFLP in 560 patients with cerebral infarction and 550 control subjects in northern China. Statistic analysis was conducted using the SPSS 10.0 program. The linkage disequilibrium (LD) was analyzed by EH and SHEsis softwares. Results Three single nucleotide polymorphisms were found in intron 1 and intron 5 of ApoM gene. The frequencies of GA+AA genotype and A allele of rs805264, GT +TT genotype and T allele of rs707922, CA+AA genotype and A allel of rs707921 were significantly higher in the patients (33.8% and 17.9%, 34.5% and 18.3%, 33.4% and 17.8%, respectively) than in controls (21.1% and 11.2%, 21.8 % and 11.5%, 20.9% and 11.1%, respectively). The LD was found in rs805264, rs707922 and rs707921 of ApoM gene (χ2=2595.03, P<0.01). There was strong LD between each pair of the three markers (D′=0.972 to 0.992). Multiple logistic regression analysis revealed that the A-T-A haplotype of the ApoM gene was an independent risk factor for cerebral infarction (OR=1.780;95%CI=1.333-2.376, P<0.01). Conclusions GA genotype and A allele of rs805264, GT genotype and T allele of rs707922, CA genotype and A allele of rs707921 may be genetic risk factors for cerebral infarction. A-T-A haplotype of ApoM gene may be a susceptible genotype of cerebral infarction.

Objective　To assess the diagnostic efficacy of the cell-free Mycobacterium tuberculosis DNA testing (CF-TB) in tuberculous pleurisy. Methods　A total of 71 patients diagnosed with tuberculous pleurisy and 35 patients with non-tuberculous pleurisy were selected from the Fifth People's Hospital of Wuxi between January to December 2022. The standard pleural puncture was conducted to collect pleural effusion, which was then utilized for Mycobacterium tuberculosis culture, GeneXpert Mycobacterium tuberculosis/rifampicin resistance (GeneXpert MTB/RIF), CF-TB, and adenosine deaminase (ADA) testing. Blood samples were subjected to tuberculosis infection T-cell spotting test (T-SPOT.TB) assay. The receiver operator characteristic curve (ROC) was applied to obtain the optimal cut-off value for pleural fluid CF-TB and to compare the diagnostic performance of CF-TB with other methods. Data analysis was conducted using SPSS 22.0 software, with statistical significance defined as P<0.05. Results　The ROC curve analysis determined that the optimal cycle threshold (Ct) value for diagnosing tuberculous pleurisy using CF-TB in pleural fluid was 38.489, with a sensitivity of 91.5% and specificity of 97.1%. In comparison, the sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of T-SPOT.TB and pleural fluid ADA in diagnosing tuberculous pleurisy were 86.0%, 71.4%, 86.0%, 71.4%, 81.1%, and 55.0%, 91.4%, 92.9%, 50.8%, 67.0%, respectively, all of which were lower than the diagnostic efficiency of CF-TB. Furthermore, the specificity of pleural fluid CF-TB in diagnosing tuberculous pleurisy (97.1%) was not significantly different from GeneXpert MTB/RIF (100%) and Mycobacterium tuberculosis culture (100%), but its sensitivity (91.5%) was significantly higher than both GeneXpert MTB/RIF (19.7%) and Mycobacterium tuberculosis culture (28.2%), with a statistically significant difference (P<0.001). Conclusions　Compared to the conventional gold standard for diagnosing tuberculous pleurisy, CF-TB exhibits a higher sensitivity and its specificity is superior to that of tuberculosis immunological test. Consequently, CF-TB can serve as a valuable complement to other traditional detection methods in aiding the diagnosis of tuberculous pleurisy.

Objective To explore the ultrasound comparison of PCOS during pre-and pro-cure of laparoscopic ovarian drilling.Methods 14 PCOS cases of the application of pre-and pro-laparoscopic ovarian drilling ovarian were monitored. The changes in the structure,colorful Doppler blood flow characteristics and blood flow parameters changes were observed. Results Significant improvement was observed in post-operative ovarian morphology,ovarian artery blood flow parameters. Conclusion Transvaginal colorful Doppler before and after laparoscopic ovarian drilling has played an indispensable role in monitoring ovarian morphology and blood flow parameters changes on the recovery of ovarian review,evaluating the effect of surgery and the monitoring of ovulation,guiding pregnancy in patients to improve the pregnancy rate.

The pathogenesis of tourette syndrome is not clear yet, and the clinical manifestations are diverse. With modern medi-cal treatment, adverse reactions occur frequently in clinics. Based on syndrome differentiation, TCM treatment can well control the clinical symptoms of children, and achieve better results without obvious adverse reactions. TCM treatment has unique advantages. In the paper, the etiology and pathogenesis, and the classification of the disease were discussed, so as to systematically explore TCM treatment of the disease and provide reference for TCM treatment of the disease.

OBJECTIVETo observe the effects of intervention of moxa smoke with different concentrations on superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and lung of male rats, so as to explore the safety concentration of moxa smoke.

METHODSA total of 32 Wistar male rats were randomly divided into a control group, a low-concentration group, a moderate-concentration group and a high-concentration group, 8 rats in each one. All the rats were exposed in the full-automatic toxicant exposure cabinet, and the overshadow of moxa smoke was set at 0%, 10%, 40% and 70%, respectively. Each rat was exposed for 20 min per day. After 26 weeks, the activities of SOD and content of MDA in serum, lung organ and bronchoalveolar lavage fluid were tested.

RESULTSCompared with the control group, the activities of serum SOD in the high-concentration group were reduced (P< 0. 05), but those in the low-concentration group and moderate-concentration group were not significantly different (both P>0. 05). Compared with the control group, the content of serum MDA in the low-concentration group, moderate-concentration group and high-concentration group was increased insignificantly (all P>0. 05). There were no significant differences regarding activities of SOD and content of MDA in lung organ and bronchoalveolar lavage fluid among each moxa smoke group (all P>0. 05).

CONCLUSIONThere is no obvious toxic reaction in the low-concentration group and moderate-concentration group; in the high-concentration group the antioxidant ability is damaged due to long-term exposure.

Animals ; Artemisia ; chemistry ; Lung ; enzymology ; metabolism ; Male ; Malondialdehyde ; blood ; metabolism ; Moxibustion ; Rats ; Rats, Wistar ; Smoke ; analysis ; Superoxide Dismutase ; blood ; metabolism