Objective To determine the clinical value of cervical conization in diagnosis and treatment of cervical intraepithelial neoplasia(CIN)and early cervical Cancer.Methods The clinical data of 142 patients who underwent cervical conization and vaginoscope multiple biopsies were analyzed retrospectively,and the results and complications were compared.Results There was a correlation in pathology cervical conization and vaginoscope multiple biopsies in 70 cases(49.3%,70/142).The major complication of cervical conization was hemorrhage and cervix adherence.The percentage of cervix adherence was 2.8%(4/142).The blood volume<20ml,20-100ml,101-200ml and>200ml were 125,10,6 and 1 case respectively.Conclusions Cervical conization plays a very important role in diagnosis of CIN and early cervical cancer.It is simple,short time,little hemorrhage and high excision rate.

OBJECTIVE:To observe the efficacy and safety of Compound sabal berry tablet combined with tamsulosin in the treatment of benign prostatic hyperplasia complicated with urinary obstruction. METHODS:86 patients with benign prostatic hyper-plasia complicated with urinary obstruction were randomly divided into control group(43 cases)and observation group(43 cases). Control group orally received 0.2 mg Tamsulosin hydrochloride orally disintegrating sustained-release tablet,once every evening. Observation group additionally received 500 mg Compound sabal berry tablet before a meal,3 times a day. 4-week was regarded as 1 treatment course,and it lasted 3 courses. During the treatment,all patients in the two groups ate light food,avoiding spicy and bland food. The clinical efficacy,TCM symptom scores,interleukin-8 (IL-8),interleukin-10 (IL-10),tumor necrosis factor α(TNF-α),maximum flow rate(Qmax),post-void residual(PVR),the International Prostate Symptom Score(IPSS)scores before and after treatment and the incidence of adverse reactions in the 2 groups were observed. RESULTS:The total effective rate in ob-servation group was significantly higher than control group,the difference was statistically significant(P<0.05). Before treatment, there were no significant differences in the TCM symptom scores,Qmax,PVR,IPSS scores,IL-8,IL-10 and TNF-α between 2 groups (P>0.05). After treatment,TCM symptom scores,IPSS scores,IL-8,IL-10,TNF-α and PVR in 2 groups were signifi-cantly lower than before,and observation group was lower than control group,Qmax was significantly higher than before,and obser-vation group was higher than control group,the differences were statistically significant (P<0.05). And there were no severe ad-verse reactions during treatment. CONCLUSIONS:The efficacy of Compound sabal berry tablet combined with tamsulosin is superi-or to tamsulosin alone in the treatment of benign prostatic hyperplasia complicated with urinary obstruction,it can reduce the in-flammatory reactions and residual urine,with good safety.

As a series of multifunctional cells,recent studies indicate that most of the CD4 + T lymphocyte subsets involve in the tumor angiogenesis and invasion,the apoptosis of tumor cells,and the expressions of acute phase proteins and cancer-promoting genes by activating or inhibiting the innate immune cells,the adaptive immune cells and non-immune cells,thus function as tumor promoters or inhibitors in hepatocellular carcinoma (HCC).

Objective To observe the effects of elevated temperatures(37~44.5℃)on the cell counts, hemolysis and osmotic fragility of stored red blood cells.Methods After 7~35 days of storage at 4℃, erythrocyte suspensions were heated by incubation at 37℃ and 44.5℃ for 30 min in an incubator and then examined for cell counts, hemolysis and osmotic fragility.Results Red blood cell, white blood cell and platelet counts, hemoglobin and potassium were unchanged following the heat treatment. In the osmotic fragility test, hemolysis remained within normal limits. Hemolysis of groups incubated at 37℃ and 44.5℃ began at (4.41?0.38)g/L and (4.3?0.2)g/L respectively while a complete hemolysis occurred at (3.42?0.36)g/L and (3.4?0.1)g/L , showing no significant difference compared to the room temperature control (4.5?0.18)g/L and (3.6?0.1)g/L. There was no significant distinction in the proportions of smooth spherocyte among the samples incubated at different temperatures, except the samples stored for 35 days, probably due to the long storage period.Conclusions Heating at temperatures between 37～44.5℃ for as long as 30 min does not cause hemolysis or other damage to red blood cells.

Objective To discuss the practice of nursing management model of stroke unit based upon the international standards implemented in the neurological unit.Methods The experiences of the stroke unit management were summarized and applied to instruct the clinical practice.Results After the implementation,the average hospitalization days,the proportion of drugs,medical cost,and mortality rate decreased.The satisfactory degree of patients with the stroke unit increased.Conclusions The nursing management model of stroke unit not only improved the quality of nursing service to stroke patients,but also decreases the mortality and disability rate,shortened the length of hospital stay,and enhanced the satisfaction degree of patients and their family members.

Objective To analyze the relationship between WT1 gene polymorphism rs16754 and prognosis of acute myeloid leukemia (AML) by meta-analysis. Methods Studies published in PubMed, CNKI, WanFang and CBM database were searched by using the search terms 'WT1', 'Polymorphism' and 'Leukemia, Myeloid, Acute', respectively. Deadline was December 1, 2015. Results A total of 11 English articles with a total of 2 789 patients were included. Meta-analysis showed that there was no association between polymorphism rs16754 and complete remission of AML patients (RR=1.02, 95 % CI: 0.99~1.06, P=0.20), overall survival(OS) (HR=0.68, 95 % CI: 0.45~1.02, P=0.06), 5-year OS (RR=1.10, 95 % CI:0.90~1.34, P=0.37) or relapse-free survival (RFS) (HR=0.80, 95 % CI: 0.54~1.19, P=0.27). Conclusion There is no correlation between WT1 gene polymorphism rs16754 and the prognosis of AML.

The curative effects of multi-disciplinary and comprehensive treatments for colorectsl cancer have been widely recognized in recent years.Because of the diversification of colorectal cancer treatment and the relatively mature technology,the current treatment of colorectal cancer could embody the multi-disciplinary and comprehensive treatment.The curative effect of neo-adjuvant chemoradiotherapy has been validated by several recent clinical trials.Other treatment methods such as Chinese medicine,radiofrequency ablation and hepatic artery infusion therapy are also involved in the comprehensive treatment,which provide considerable curative effects.

AIM:To investigate whether hypoxic preconditioning(HPC)protects cardiomyoblast H9c2 cells against oxidative injury,and to discuss whether calreticulin(CRT)contribute to this protection through p38 MAPK signaling pathway.METHODS:Cardiomyoblast H9c2 cells were randomly divided into eight groups as follows:hydrogen peroxide stress(H2O2);brief hypoxic exposure of 20 min to simulate hypoxic preconditioning(HPC);20 min of hypoxic exposure followed by 24 h of normoxic reoxygenation before hydrogen peroxide stress(HPC+H2O2),SB203580(the specific inhibitors of p38 MAPK)+HPC+H2O2,antisense oligonucleotides transfection of calreticulin(AS),AS+H2O2,AS+HPC+H2O2 and control.Morphological studies,estimation of lactate dehydrogenase(LDH)leakage and flow cytometry were employed to assess the cell apoptosis and necrosis.RT-PCR and Western blotting analysis was used to detect calreticulin expression and phosphorylation of p38 MAPK.RESULTS:The results obtained are as follows:(1)HPC relieved cell injury caused by H2O2.Compared with those in H2O2 group,apoptosis rate and LDH leakage in culture medium in HPC + H2O2 group decreased 13.4% and 44.0%,respectively(P

Objective To evaluate the effects of four different intravenous anesthetic agents on middle cerebral artery blood flow velocity(V MCA) measured with transcranial Doppler sonography (TCD) during induction of general anesthesia Methods Fourty patients were classified randomly into four groups (n=10 each) according to intravenous anesthetic agents used: propofol group (group P, propofol 2 0 mg/kg); etomidate group (group E, etomidate 0 3 mg/kg); midazolam group (group M, midazolam 0 15 mg/kg) and thiopental group (group T, thiopental 5 0 mg/kg) Anesthesia was induced with one of above drugs followed by endotracheal intubation and maintained with sevoflurane and nitrous oxide in oxygen The lungs were ventilated to maintain an end tidal PCO 2 of 35 40 mmHg The V MCA, arterial blood pressure (MAP), heart rate (HR) and end tidal PCO 2 were measured and recorded at following occasions: before induction, 1, 3, 5, 10 and 15min after induction Results There were no significant differences in preinduction data (patient age, body weight, sex, MAP, HR, and V MCA) among four groups Compared with the baselines, V MCA decreased by 34 4% in group P, by 34 2% in group E, and by 31 2% in group T(P0 05)1 min after the administration; immediately after the endotracheal intubation, V MCA increased markedly in group M (P0 05) Conclusions The cerebral artery blood flow velocity can be affected differently by various induction agents

Objective To explore the causes of an outbreak of 168 cases of incisional infection in one hospital.Methods The secretory substances of incisioral wound infection from 168 cases of operation patients were collected,cultured and identified in bacterium strain.We also did drug sensitivity test,pathological examination and investigation of environment.Results Above these experinental studies demostrated that the large-scale outbreak of incisiona infection was caused by mycobacterium chelonei in operation room.Used 0.137% glutaradehyde as disinfectant immersion surgical tools ,but the concentration too low to kill the mycobacterium chelonei,which polluted the hickeyes of surgical instrument.The pollution leading to outbreak of incisional infection.Conclusions By reason of the event lesson,authors suggest that the doctors and nurses should strengthen the detection of atypical mycobacterium,so as to reduce the iatrogenic infection,reducing,or stoping the outbreak of incisinal Infection.