Objective To investigate the difference of TCM body constitution betweenpeople of the Shimian county and Chengdu city. Methods 200 sub-health patients in clinic of Shimian TCM Hospital were selected randomly. Another 200 sub-health patients in clinic of the Second Sichuan Province Hospital of TCM wererandomly selected. The two groups were identified with TCM body constitution based on the clinical symptoms, tongue, pulse condition, etc. Comparisons on body constitution were made between the two groups. Results In Chengdu, patients withbalance constitution and Qi deficiency constitution (respectively have the proportion of 33.0% and 20.0%)were significantly higher than thoseof Shimian County(the proportion of the two wererespectively 4.0% and 6.0%);while Shimian County have a significantly higher ratio of phlegm dampness and blood stasis constitution(43.5% and 13.0%)than those in Chengdu city(the proportion of the two wererespectively 8.0%and 4.0%), P<0.01. Conclusion Because of different regions, the population constitution ratio wasdifferent, so the disease tendency wasalso somewhat different. Therefore, physicians should give full consideration to the regional factors in diagnosing and treating patients.

Purpose:To investigate the treatment effect and toxicity of regimen containing ifosfamide (IFO) and etoposide (VP16) on relapse ovarian carcinoma.Methods:IFO 2.0 in 500 ml normal saline day 1—5, VP16 100 mg in 500 ml normal saline d1,3,5 Results:The overall response rate was 38.5%, mylosuppression and alopecia were the most common toxicities. Leukopenia rate was 71.4%, alopecia rate was 100%, gastro intestinal and nervous toxicity were mild. Conclusions:Combination of IFO and VP16 was very effective on relapse ovarian carcinoma with mild toxicity and should be widely used.

Recently, along with the harmful factors in air in-creasing, the incidence of respiratory system diseases has been gradually rising. Therefore, more studies are focused on impro-ving the prevention and treatment on respiratory system diseases. Current researches have shown that microRNAs ( miRNA ) con-tribute to the prevention and treatment on respiratory system dis-eases, including virus infectious respiratory disease, pulmonary fibrosis, asthma, COPD, pulmonary tuberculosis and lung canc-er. The up-regulation and down-regulation of microRNAs could effectively regulate the biological process of these diseases. The review analyzes the recent years′study results of respiratory sys-tem diseases and microRNAs in depth, and discuss the role of microRNA in the treatment and prevention of respiratory disea-ses. Moreover, in this review we can provide new knowledge a-bout respiratory diseases and development of more potent drug targets for the treatment and prevention of respiratory diseases.

Objective To analyze the relationship between WT1 gene polymorphism rs16754 and prognosis of acute myeloid leukemia (AML) by meta-analysis. Methods Studies published in PubMed, CNKI, WanFang and CBM database were searched by using the search terms 'WT1', 'Polymorphism' and 'Leukemia, Myeloid, Acute', respectively. Deadline was December 1, 2015. Results A total of 11 English articles with a total of 2 789 patients were included. Meta-analysis showed that there was no association between polymorphism rs16754 and complete remission of AML patients (RR=1.02, 95 % CI: 0.99~1.06, P=0.20), overall survival(OS) (HR=0.68, 95 % CI: 0.45~1.02, P=0.06), 5-year OS (RR=1.10, 95 % CI:0.90~1.34, P=0.37) or relapse-free survival (RFS) (HR=0.80, 95 % CI: 0.54~1.19, P=0.27). Conclusion There is no correlation between WT1 gene polymorphism rs16754 and the prognosis of AML.

66 cases were treated with Yizhi(Intellingencebenefitted)Syrup. After treatment, the scores on behavioral appraisal markedly decreased with dramatic upgrading of learning results, marked lowering of positive soft nerve signs, the total effective rate being 84.8%. There wore also marked increase in the levels of urine NE, DA, 3-4(?)PAC, CAMP and(?)r.

Objective To investigate the effect of metronidazole and tinidaxole in breast milk on infants and their concentrations. Methods The concentration of metronidazole and tinidazole inbreast milk was assayed by high performance liquid chromatography(HPLC). Results The pharmacokineticparameters(mean?s) t max(h), t 1/2ke of Metronidazole(20 mg/kg, n=8 ) and Tinidazole (13 mg/kg,n=7) in lactation women infused with a single dose were(1.7?1.0)h、(20?5) mg/L and (1.3?0.6)h、(17?3) mg/L respectively. The total amount of metronidazole and tinidazole ingested by the infant via breast milk were 3.02 mg/kg/day and 2.58 mg/(kg?d) respectively. which were 1/5~1/10. and 1/6~1/8 of the routine therapeutic dose for infants.

OBJECTIVE:To investigate the current situation and developing trend of the use of antipsychotics.METHODS:The data of antipsychotics in Analysis System of Drug-use in the Hospitals of the Yangtze River Valley,reported by Hangzhou,Shanghai,Nanjing,Wuhan,Chengdu and Chongqing during the period 1997～2001,were analysed by using DDD as index.RESULTS:The sum of money and frequency of use of the antipsychotics were increasing year by year;The first generation antipsychotics still occupied the marketing predominance;The use of the second generation antipsychotics assumed an increasing tendency year by year.CONCLUSION:The marketing of antipsychotics will further expand in China and the pharmaceutical factories should strengthen the development of new antipsychotics.

Objective:To investigate the effect of cPLA 2(cytosolic PLA 2, 85-kD PLA 2) on the signaling pathways of IL-1?、IL-6 release in LPS induced Hela cells.Methods:①LPS stimulated Hela cells were exposed to the complex of cPLA 2 initiation site-directed antisense oligonucleotide and liposome in the indicated doses and time, and then,analyzed the changes of cPLA 2 both in mRNA and in protein by RT-PCR and Western blot.②At the same time, the supernatants were collected to detect the level of IL-1? and IL-6 under radioimmunoassay(RIA).Results:①The transfection led to a dose-dependent decrease in cPLA 2 protein, while the change of mRNA is not notable.②The levels of IL-1?、IL-6 in the transfected cells were reduced following the repression of cPLA 2 compared with that of LPS added only.Conclusion:cPLA 2 probably plays an important role in the signaling mechanisms on IL-1?、IL-6 release in LPS-induced Hela cell. [

AIM To explore the inhibitory effects of simv astatin on ROS generation and cardiac myocytes hypertrophy induced by ET-1. METHODS The study was performed with primary cultured neonatal rat cardiac myocytes. Intrace llular fluorescence signal was assayed by fluorescence convert microscope. The l evel of intracellular ROS was measured by the ROS-specific probe 2′, 7′-dich lorofluorescin diacetate (DCF-DA). The RNA content was determined by RNA-sensitive fluoresce nce probe propidium iodide (PI) and the total protein of cell was measured by th e methods of coomassie brilliant blue. The cell surface area was measured by ima ge analysis program. RESULTS ①Fluorescence intensity of intracel lular DCF-DA and myocyte hypertrophy increased by ET-1 in dose-dependent mann er. Antioxidant catalase(0 2 U?L -1 ) attenuated the ET-1-induced incre ase of fluorescence intensity of intracellular DCF-DA and myocyte hypertrophy.②Simvastatin inhibited the increase of fluorescence intensity of intracellular DCF-DA and cardiac myocyte hypertrophy induced by ET-1(10 -8 mol?L -1 )in a dose-dependent manner. CONCLUSION ET-1 increases int racellular ROS in the cultured neonatal rat cardiac myocytes. Simvastatin inhibi ted the generation of ET-1-induced ROS and cardiomyocytes hypertrophy.

Optic nerve is composed by the axonal of retinal ganglion cells,there are no Schwann cells around it,and optic nerve belongs to the central nervous,so it cannot regenerate when injuried. In 1985 So and Aguayo have made a success in peripheral nerve retinal transplant,which completely changes the concept that optic nerve injury cannot regenarate. Currently nerve injury recovry has following several methods: use of neurotrophic factor,such as nerve growth factor,ciliary neurotrophic factor and brain-derived neurotrophic factor,etc. These factors could promote the regeneration and repair of retinal ganglion cells; treatment of gene correction and gene regulation,by changing the expression of host gene,reduce the effect due to the disease,slow the progress of the disease course or provide protection against the disease; neural stem cell transplantatin and tissue-engineered Schwann cell transplantion. But these treatments are still at the stage of animal experiment,how timely and effctively reduce the apoptosis and increase the survival rate of ganglion cells,even further promote nerve regeneration and recovery,there is still not a perfect method so far.