BACKGROUND:Currently, the implant surface is optimized to promote the osseointegration of artificial dental implants, which has become a research focus in recent years. But the femtosecond laser is less reported to modify the titanium surface so as to promote fibroblast proliferation and differentiation. OBJECTIVE: To observe the effect of femtosecond laser on the proliferation and differentiation of human periodontal ligament fibroblasts on titanium surface. METHODS: Human periodontal ligament fibroblasts were respectively inoculated on different titanium surfaces of the three groups (femtosecond laser group, TiO2 particle blasted group and pure titanium group). Then differentiation and proliferation of human periodontal ligament fibroblasts were observed. RESULTS AND CONCLUSION:There was a significant difference in the surface roughness among these three groups, and compared with the pure titanium group, the attached cels were more in the femtosecond laser group and TiO2 particle blasted group. mRNA expressions of RUNX2 and OSX were highest in the femtosecond laser group folowed by TiO2 particle blasted group and pure titanium group in order. These findings indicate that pure titanium treated with femtosecond laser has fine biological property and may be more feasible for human periodontal ligament fibroblasts to attach, grow and proliferate.

ObjectiveTo evaluate effect of music electrical stimulation on functional headache . Method78 patients were selected randomly from the patients who were cured after two years. Result46 patients are cured completely, 18 patients get better than before, accounting for 82.05%. Conclusion Music electrical stimulation is effective for functional headache.

BACKGROUND:Tissue-engineered bone, as an emerging method, is used to repair bone defects, but it is difficult to be used widely because of no nutrient metabolism of implants. Pre-vascularized tissue-engineered bone research is performed for this limitation. Forward-looking and procedural vascular construction prior to artificial grafting is perspective to supply the nutrient metabolism of implants.
OBJECTIVE:To analyze the research results and developmental tendency of pre-vascularized bone engineering in vitro using a multilevel analysis.
METHOD:Literature search was performed in CNKI database for Chinese literatures and PubMed database for English literatures from 2000 to 2012. The key words included“tissue-engineered bone, vascularization, implant, osteoblasts, endothelial cel s, co-culture”in Chinese, and“bone engineering, endothelial cel s, osteoblast, implant, cel s co-culture”in English. A review addressing pre-vascularized tissue-engineered bone was completed based on retrieved literature classified according to bone physiological studies, in vitro experimental research and material research.
RESULTS AND CONCLUSION:Total y 60 articles were included in result analysis. Studied have documented that microvascular regeneration in normal bone tissue has an important role in osteogenesis regulation. Current bone tissue engineering research is to simulate this physiological process of vascular regeneration in the human body, and lots of in vitro studies are designed to complete this angiogenic process in bone tissue engineering. It is confirmed that in vitro pre-vascular work is used for bone tissue engineering, especial y for in vivo survival and ossification of tissue-engineered bone, pointing out the developmental direction for the clinical application of tissue-engineered bone.

OBJECTIVE To evaluate the effectiveness of applying neuronal stem cells to bridging a 10mm long facial nerve defect in rabbits using a tissue engineered artificial nerve. METHODS Thirty-six Japanese white rabbits were randomly divided into three groups of 12. Group A: chitosan guidance channels with collagen protein sponge were filled with neuronal stem cells and NGF; Group B: chitosan guidance channels with collagen protein sponge were filled with NGF; Group C: autograft nerve. A series of examinations were performed, including electrophsiological methods, histological staining of nerve, Brdu and S100 immunohistochemical staining, at 12 weeks after operation. RESULTS At 12 weeks after operation, all examination results from Group A were better than from Group B(P0.05). CONCLUSION Neuronal stem cells can be used as seeding cells in peripheral nerve tissue engineering for repairing 10mm facial nerve defects.

OBJECTIVE:To observe the clinical efficacy of Tanreqing injection in the treatment of children's viral pneumonia.METHODS:A total of sixty children with viral pneumonia were randomly divided into 2 groups:the treatment group(n=30)were treated with Tanreqing injection(20mL)plus Glucose(250mL)qd for 7d while the control group(n=30)with Cibavirin(10～15mg?kg-1)plus Glucose(250mL)bid for 7d.The primary outcome measures were clinical efficacy and adverse drug effects(ADRs).RESULTS:The effective rate was 100% in the treatment group as compared with 80% in the control group,showing significant differences between the two groups(P

With the rapid development of new media,medical students' ideological and moral qualities,physical and mental health have been unprecedentedly affected.Traditional ideological and political education modes for medical students encounter great challenges.In order to strengthen the ideological and political education for medical students,we need to focus on three aspects,namely enhancing information qualities of the subjects of ideological and political education,enhancing the supervision of public opinion and guidance on new media and building new interactive environment.

Objective To identify the risk factors associated with intraoperative acute cardiac decompensation in patients undergoing off-pump coronary artery bypass grafting (OPCABG). Methods From November 2007 to February 2009, 2379 OPCABGs were performed in our hospital. The possible risk factors associated with intraoperative cardiac decompensation were retrospectively analyzed. The preoperative patient demographics and intraoperative characteristics were correlated with intraoperative acute cardiac decompensation.The possible risk factors included sex, age, body weight, cardiac function (NYHA classification), the associated diseases (hypertension, diabetes mellitus, liver-kidney dysfunction), history of myocardial infarct, ventricular aneurysm, preoperative treatment with β-blocker and/or calcium channel blocking agent, ventricular extrasystole,atrial fibrillation, duration of operation, etc. Results Three hundred and sixty-eight patients developed acute cardiac decompensation during OPCABG (15.5%). No patient died during operation. Multivariate analysis indicated that the risk factors for acute cardiac decompensation during OPCABG included left ventricular aneurysm valvular dysfunction, left main disease, history of myocardial infarct, preoperative ventricular premature beat,preoperative ejection fraction (EF) ＜ 40%, intraoperative atrial fibrillation, intraoperative frequent ventricular premature beat, tachycardia before anesthesia and emergency OPCABG. Conclusion The risk factors for acute cardiac decompensation during OPCABG includ left ventricular aneurysm valvular dysfunction, left main disease,history of myocardial infarct, preoperative ventricular premature beat, preoperative EF ＜ 40%, intraoperative atrial fibrillation, intraoperative frequent ventricular premature beat, tachycardia before anesthesia and emergency OPCABG.

The aim of the study is to evaluate the feasibility and safety of Bispectral index (BIS) monitoring in pediatric radio frequency catheter ablation. One hundred and six children aged 0. 6-12 years, scheduled for radio frequency catheter ablation, were randomly divided into two groups. In group A patients received BIS monitoring during the operation (n = 50), and the group B received modified Observer's Assessment of Alertness/Sedation (OAA/S) scaling (n = 56). The anesthesia was maintained with propofol target-controlled infusion. The intraoperative propefol target concentration was adjusted to maintain the BIS values between 55-65 in group A and OAA/S scale about 1 in group B respectively, The heart rate (HR), mean arterial pressure (MAP) and pulse oximetric saturation (SpO2) were measured before anesthetic induction, 1 min after induction, catheter puncturing and the end of operation respectively. The requirements of propofol, the times of supporting ventilation and recovery, the respiratory depression, nausea and vomiting postoperatively were also recorded. The intraoperative HR, MAP and SpO2 showed no differences between two groups, but the requirements of pmpofol, the times of supporting ventilation and recovery were less in group A than that of group B (P<0.05). All children didn't have nausea, vomiting and respiratory depression. The results suggest that in pediatric radio frequency catheter ablation, BIS monitoring has the advantages of timely adjustment of anesthetic depth, reducing anesthetic requirements, shortening the time of recovery, so as the perioperative safety can be improved.

ObjectiveTo discuss the perioperative nursing of sigmoid colon cystoplasty in neurogenic bladder patients after spinal cord diseases. Methods31 patients with neurogenic bladder due to the spinal cord diseases and received sigmoid colon cystoplasty were reviewed and followed up. ResultsThere were 2 cases complicated with bowel obstruction, 1 case with acute alleosis and 1 case with mycotic infection in bladder after the operation, who were all cured. After discharge, 2 cases lost the follow-up. During the follow-up period of 6 months to 4 years, 29 patients recovered well and kept in self-intermittent catheterization. ConclusionPreoperative psychological nursing and intestinal and bladder preparation, postoperative gastrointestinal and urinary drainage catheters nursing, intermittent catheterization training, regular follow-up and health guiding were the key nursing contents and the important step for ensuring an excellent outcome for the operation.

Objective To determine if the cardiac index (CI) measured with arterial pressure-based cardiac output (APCO) agrees with that measured with pulmonary artery catheter (PAC) during cesarean section in parturients with congenital heart disease and severe pulmonary hypertension.Methods Thirty parturients with congenital heart disease and severe pulmonary hypertension,scheduled for elective cesarean section,were enrolled in the study.APCO and PAC were used for cardiac output and other parameters of hemodynamics monitoring during operation in all patients.Continuous epidural anesthesia was performed.CI was recorded immediately before epidural administration (T1),at 5 and 10 min after epidural administration (T2-3),immediately before delivery (T4),and at 2 and 5 min after delivery (T5-6).The correlation was tested by Pearsone correlation analysis.CI derived from APCO and PAC was compared by Bland-Altman analysis for agreement.Results CI measured with PAC was significantly higher than that measured with APCO (P ＜ 0.05).Compared with the baseline value at T1,no significant difference in CI measured with PAC and APCO was found at T11-T4 and T6 (P ＞ 0.05),and CI measured with PAC and APCO was significantly increased at T5 (P ＜ 0.05).Bland-Ahman analysis showed poor agreement between CI measured with the two methods.The correlation analysis showed that there was positive correlation between CI derived from the two methods at T1-6 (the correlation coefficient was 0.93,0.95,0.94,0.89,0.96 and 0.91 at T1-6,respectively,P ＜ 0.05).Conclusion CI value obtained with APCO agrees poorly with that obtained with PAC during cesarean section in parturients with congenital heart disease and severe pulmonary hypertension,but agrees well in monitoring the changing trend of CI.