The regulatory processes in developmental biology research are significantly influenced by long non-coding RNAs (lncRNAs). However, the dynamics of lncRNA expression during human tooth development remain poorly understood. In this research, we examined the lncRNAs present in the dental epithelium (DE) and dental mesenchyme (DM) at the late bud, cap, and early bell stages of human fetal tooth development through bulk RNA sequencing. Developmental regulators co-expressed with neighboring lncRNAs were significantly enriched in odontogenesis. Specific lncRNAs expressed in the DE and DM, such as PANCR, MIR205HG, DLX6-AS1, and DNM3OS, were identified through a combination of bulk RNA sequencing and single-cell analysis. Further subcluster analysis revealed lncRNAs specifically expressed in important regions of the tooth germ, such as the inner enamel epithelium and coronal dental papilla (CDP). Functionally, we demonstrated that CDP-specific DLX6-AS1 enhanced odontoblastic differentiation in human tooth germ mesenchymal cells and dental pulp stem cells. These findings suggest that lncRNAs could serve as valuable cell markers for tooth development and potential therapeutic targets for tooth regeneration.
Humans ; RNA, Long Noncoding/metabolism* ; Odontogenesis/genetics* ; Tooth Germ/embryology* ; Cell Differentiation ; Gene Expression Regulation, Developmental ; Mesoderm/metabolism* ; Tooth/embryology* ; Gene Expression Profiling ; Sequence Analysis, RNA ; Dental Pulp/cytology*

The small-molecule alkaloid halofuginone (HF) is obtained from febrifugine. Recent studies on HF have aroused widespread attention owing to its universal range of noteworthy biological activities and therapeutic functions, which range from parasite infections and fibrosis to autoimmune diseases. In particular, HF is believed to play an excellent anticancer role by suppressing the proliferation, adhesion, metastasis, and invasion of cancers. This review supports the goal of demonstrating various anticancer effects and molecular mechanisms of HF. In the studies covered in this review, the anticancer molecular mechanisms of HF mainly included transforming growth factor-β (TGF-β)/Smad-3/nuclear factor erythroid 2-related factor 2 (Nrf2), serine/threonine kinase proteins (Akt)/mechanistic target of rapamycin complex 1(mTORC1)/wingless/integrated (Wnt)/β-catenin, the exosomal microRNA-31 (miR-31)/histone deacetylase 2 (HDAC2) signaling pathway, and the interaction of the extracellular matrix (ECM) and immune cells. Notably, HF, as a novel type of adenosine triphosphate (ATP)-dependent inhibitor that is often combined with prolyl transfer RNA synthetase (ProRS) and amino acid starvation therapy (AAS) to suppress the formation of ribosome, further exerts a significant effect on the tumor microenvironment (TME). Additionally, the combination of HF with other drugs or therapies obtained universal attention. Our results showed that HF has significant potential for clinical cancer treatment.

Objective To explore the variations and prognostic factors of hyperhomocysteinaemia in ischemic cerebral apoplexy for the youth who administrated vitamin B6,vitamin B12 and folic acid at pretherapy and post-treatment.Methods One hundred and twenty cases of young patients with ischemic cerebral apoplexy in the Pinggu Hospital of Capital University from January 2003 to December 2013 as case group(intervention group,60 cases and 60 cases of non-intervention group),while 120 youth volunteers with the same period and age without neurological diseases as a control group.Both groups patients were detected for hyperhomocysteinaemia,folic acid and vitamin B12.The non-intervention group was administrated basic treatment,while the intervention group administrated vitamin B6,vitamin B12 and folic acid on this basis.The hyperhomocysteinaemia,folic acid and vitamin B12 were detected repetitively after four weeks.Results Compared with control group,the hyperhomocysteinaemia in ischemic cerebral apoplexy group for the youth had increased significantly ((10.2 ± 3.1) μmmol/L vs.(21.3 ± 4.5) μmmol/L,P ＜ 0.05).The hyperhomocysteinaemia,folic acid and vitamin B12 had no significant differences between intervention group and non-intervention group (P ＞ 0.05).After replenished vitamin B6,vitamin B12 and folic acid,the hyperhomocysteinaemia had decreased significantly ((10.5 ± 3.0) μmnol/L) in intervention group.Folic acid ((6.5±2.8)μg/L) and vitamin B12(450.2±155.6) ng/L) had increased significantly(P＜0.05).Conclusion The hyperhomocysteinaemia increased in ischemic cerebral apoplexy for the youth.It is that hyperhomocysteinaemia decreased by replenished vitamin B6,vitamin B12 and folic acid which make for prognosis in ischemic cerebral apoplexy for the youth.

Objective To analyze the recurrence risk of patients single post-stroke epilepsy. Methods Fifty-eight cases of epilepsy after stroke in Pinggu District Hospital of Beijing were enrolled in this study and their history clinical information were retrospectively collected. All patients were divided into single attack group (A group)and two or more attack group(B group). Results There were 3. 67%(58/1580)stroke patients were developed epilepsy. Among them,0. 38%(6/58)of patients were developed seizures in early stage,and 3. 29%(52/58)in late stage. Thirty-one cases occurred epilepsy once within one years in A group and 27 cases occurred epilepsy more than twice with one year in B group. Initiate epilepsy onset of 2 cases(A1)was at early stage and 29 cases(A2)at late stage in A group. Initiate epilepsy onset of 3 case(B1)was at early stage and 24 cases(B2)at late stage in B group. There was no significant difference in term of types of epilepsy between two groups(χ2 =0. 001,P﹥0. 05). The lesions site of 13 cases was located cortex and 18 cases was located in below cortex in A group,in B group,the lesions site was located in cortex of 17 cases or below cortex of 10 cases,and the difference was not significant(χ2 =2. 555,P﹥0. 05). The hemorrhagic stroke and ischemic stroke were 12 and 19 cases in A group,10 and 17 cases in B group,and there was no significant difference between two groups (χ2 =0. 017,P﹥0. 05). Rhythm of slow wave and the epileptiform discharges were 11 and 2 cases in A group, 11 and 13 cases in B group. About 51. 8% of the patients with recurrence of epilepsy had history of infection in B group. Conclusion For stroke patients,especially hemorrhagic stroke,first seizure is late-onset epilepsy cases. If the electrical activity of the brain is abnormal slow rhythm or epileptiform discharges,close to the cortex is more likely to cause epilepsy recurrence. It is supposed to giving positive antiepileptic drug treatment in the first epilepsy after stroke.

Objective: To examine the effects of autogenic training and abdominal breathing on heart rate and finger temperature. Methods: Forty-five healthy female university students were randomly assigned to three groups:control、autogenic training and deep abdominal breathing groups, and their physiological effects were compared. Results:The result of this experiment showed that both autogenic training and abdominal breathing increased finger temperature significantly, however, the increase magnitude didn't show significant differences in two groups.During autogenic training, the heart rate of autogenic training group declined significantly,the heart rate of deep abdominal breathing group decreased significantly after deep abdominal breathing. The decrease magnitude between the three groups showed no significant differences. Conclusion: Both the present techniques can decrease heart rate and increase finger temperature.

L-asparaginase activity produced by six E. coli J53 strains containing different plasmids and plasmid free E. coli J53 strain was compared. The enzyme activity of the plasmid- bearing strains was about 2—4 times lower than that of the plasmid free ones. Curing the R plasmids from E. coli J53, the activity of L-asparaginase increased and was close to that of the plasmid free strain. It is proved that the remarkable inhibition of L-asparaginase activity results from the presence of the plasmid in E. coli.

Objective To analyze the relationship between children's respiratory health and ambient air pollution in China.Methods The studies on ambient air pollution and children's respiratory health in China published from 1980 to 2008 were collected and 12 of them were selected.Pearson correlation and linear regression analysis were used to find the correlations between levels of air pollutants and children's history prevalence of respiratory diseases and symptoms.Results Strong associations between the levels of TSP and the history prevalence of cough, long-term cough, sputum, long-term sputum, bronchitis for children were found, and 0.50%,0.12%,0.43%,0.09% and 0.51% increased respectively as per 10 ?g/m3 increased for TSP;there were significant associations between the levels of PM10 and the history prevalence of cough, sputum and bronchitis for children, and 2.64%, 2.27%,2.17% increased respectively as per 10 ?g/m3 increased for PM10.Significant associations were also found between the levels of PM2.5 and the history prevalence of cough, sputum and bronchitis for children, and 4.56%,3.49%, 3.74% increased respectively as per 10 ?g/m3 increased for PM2.5.Pearson correlation analysis indicated a significant increase of the history prevalence of wheeze for children with increase of levels of SO2;there were significant associations between the increase of the levels of SO2 and increase of the history prevalence of cough and sputum for children as the levels of SO2 lower than 0.15 mg/m3 , and 1.65% and 1.50% increased respectively as per 10 ?g/m3 increase of SO2.Significant associations between the levels of NOx lower than 0.10 mg/m3 and the history prevalence of long-term cough, long-term sputum, bronchitis for children were found, and 0.86%, 0.51% and 3.21% increased respectively as per 10 ?g/m3 increase of NOx.Furthermore, the associations between air pollutants and the history prevalence of children's respiratory health in north China were more significant.Conclusion The air pollutants in China are risk factors of children's respiratory system health, and impacts of air pollutants on children's respiratory system health in north China is more significant than that of the whole regions.