1.A clinical analysis of synthetic therapy effect and prognostic factors in 107 patients with breast cancer
Journal of Chongqing Medical University 1987;0(01):-
Objective:To investigate the synthetic therapy effect and prognosticfactorsin 107 patientswith breastcancer and explore the relationship between some clinical-pathological factors and patients' prognosis.Methods:Between Jan,2000 and Dec,2003,the clinical-pathological materials of 107 breast cancer cases who have received synthetic therapy,including radiotherapy,surgery,chemotherapy and endocrine therapy,were analyzed.The correlation of age,menopausal status,tumor size,axillary lymph nodal status,TNM stage,hormone receptor status and endocrinetherapy with prognosis of breast cancer was evaluated.Results:The overall survival and disease-free survival of the whole patients were 90.3 and 74.8 months,respectively.Analysis revealed that overall survival and disease-free survival of this group were significantly associated with tumor size,axillary lymph nodal status and TNMstage(P0.05).Conclusion:Our data demonstrate that tumor size,axillary lymph nodal status and TNM stage are significant independent indicators of prognosis in breast cancer respectively,and age,menopausal status,hormone receptor status and endocrine therapy are not significant factors of prognosis.
2.Interferon-gamma inhibits aldehyde dehydrogenasebright cancer stem cells in the 4T1 mouse model of breast cancer.
Xiufen ZHUANG ; Guilan SHI ; Xiao HU ; Huiru WANG ; Wen SUN ; Yanhong WU
Chinese Medical Journal 2021;135(2):194-204
BACKGROUND:
Despite improvements in disease diagnosis, treatment, and prognosis, breast cancer is still a leading cause of cancer death for women. Compelling evidence suggests that targeting cancer stem cells (CSCs) have a crucial impact on overcoming the current shortcomings of chemotherapy and radiotherapy. In the present study, we aimed to study the effects of T cells and a critical anti-tumor cytokine, interferon-gamma (IFN-γ), on breast cancer stem cells.
METHODS:
BALB/c mice and BALB/c nude mice were subcutaneously injected with 4T1 tumor cells. Tumor growth and pulmonary metastasis were assessed. ALDEFLOUR™ assays were performed to identify aldehyde dehydrogenasebright (ALDHbr) tumor cells. ALDHbr cells as well as T cells from tumor-bearing BALB/c mice were analyzed using flow cytometry. The effects of CD8+ T cells on ALDHbr tumor cells were assessed in vitro and in vivo. The expression profiles of ALDHbr and ALDHdim 4T1 tumor cells were determined. The levels of plasma IFN-γ were measured by enzyme-linked immunosorbent assay, and their associations with the percentages of ALDHbr tumor cells were evaluated. The effects of IFN-γ on ALDH expression and the malignancy of 4T1 tumor cells were analyzed in vitro.
RESULTS:
There were fewer metastatic nodules in tumor-bearing BALB/c mice than those in tumor-bearing BALB/c nude mice (25.40 vs. 54.67, P < 0.050). CD8+ T cells decreased the percentages of ALDHbr 4T1 tumor cells in vitro (control vs. effector to target ratio of 1:1, 10.15% vs. 5.76%, P < 0.050) and in vivo (control vs. CD8+ T cell depletion, 10.15% vs. 21.75%, P < 0.001). The functions of upregulated genes in ALDHbr 4T1 tumor cells were enriched in the pathway of response to IFN-γ. The levels of plasma IFN-γ decreased gradually in tumor-bearing BALB/c mice, while the percentages of ALDHbr tumor cells in primary tumors increased. IFN-γ at a concentration of 26.68 ng/mL decreased the percentages of ALDHbr 4T1 tumor cells (22.88% vs. 9.88%, P < 0.050) and the protein levels of aldehyde dehydrogenase 1 family member A1 in 4T1 tumor cells (0.86 vs. 0.49, P < 0.050) and inhibited the abilities of sphere formation (sphere diameter <200 μm, 159.50 vs. 72.0; ≥200 μm, 127.0 vs. 59.0; both P < 0.050) and invasion (89.67 vs. 67.67, P < 0.001) of 4T1 tumor cells.
CONCLUSION
CD8+ T cells and IFN-γ decreased CSC numbers in a 4T1 mouse model of breast cancer. The application of IFN-γ may be a potential strategy for reducing CSCs in breast cancer.
Aldehydes
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Animals
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Breast Neoplasms
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Cell Line, Tumor
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Female
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Humans
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Interferon-gamma
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplastic Stem Cells
3. The therapeutic effect of HSV1-hGM-CSF combined with doxorubicin on the mouse breast cancer model
Xiufen ZHUANG ; Shuren ZHANG ; Binlei LIU ; Jiliang WU ; Xiaoqin LI ; Hangang GU ; Yang SHU
Chinese Journal of Oncology 2018;40(3):178-185
Objective:
To evaluate the oncolytic effect of herpes simplex virus type 1 which carried recombined human granulocyte-macrophage colony-stimulating factor (HSV1-hGM-CSF) on the mouse breast cancer cell line 4T1 and compare the anticancer effects of HSV1-hGM-CSF, doxorubicin alone or combination on the breast cancer in mice.
Methods:
We investigated the cytotoxic effect on 4T1 cells