1.Water channel protein AQP3 expression in activated human T lymphocytes
Xiufang KAN ; Lifeng LIU ; Jianguo ZHU ; Dan ZHAO
Chinese Journal of Immunology 1985;0(05):-
Objective:To study the expression and physiological significance of water channel proteins in human T lymphocytes.Methods:AQP0-9 mRNA and protein in inactivated and activated human T lymphocytes were analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR) analysis and Western blot.Results:No AQP was expressed in inactivated lymphocytes.However,AQP3 mRNA and protein were expressed in PHA activated T lymphocytes.Conclusion:Water and glycerol transport mediated by AQP3 may be important in T lymphocyte activation.
2.Prevalence and Relevant Risk Factors of Low Blood Pressure in China
Xigui WU ; Guangyong HUANG ; Jiangong ZHAO ; Xiufang DUAN ; Dongfeng GU
Chinese Journal of Hypertension 2001;9(1):11-13
Objective To study the prevalence and relevant risk factors of low blood pressure and its influence on cardiovascular diseases in China. Methods A cross-sectional study for low blood pressure was conducted using the data of the third national survey of blood pressure in 1991. Low blood pressure was defined for systolic (SBP) and diastolic (DBP) pressure less than the tenth percentile for the sample (SBP≤98mmHg and DBP≤60mmHg). Results The prevalence of low blood pressure for males and females were 2.7% and 7.4%, respectively. The prevalence of low blood pressure for females was higher than that of males (P<0.01). Prevalence of low blood pressure was 7.62%, 6.97%, 4.40%, 3.22%, 2.65%, 1.94% for age groups 15~, 25~, 35~, 45~, 55~, 65~, respectively. The prevalence of low blood pressure was gradually declined with aging. There was large variation in the prevalence of low blood pressure for different minorities. The prevalence of low blood pressure was higher in the southern rather than the northern part of China. Logistic regression showed that the age (OR 0.97, 95%CI 0.97~0.98, per 10 mmHg), BMI (OR 0.80, 95%CI 0.80~0.81), drinking (OR 0.89, 95%CI 0.86~0.93) and heart rate (OR 0.981, 95%CI 0.980~0.982) had reverse relationship with low blood pressure. The sex had a positive relationship with low blood pressure. The prevalence of stroke and myocardial infarction in the population with low blood pressure was lower than that of normal pressure or hypertension (P<0.01). Conclusion The prevalence and relevant risk factors of low blood pressure in China were various. We speculate that low blood pressure in Chinese population appears to be a normal state of physiology.The difinite influence in health needs cohort studies to confirm.
3.Study on the quality management of biological variation allowed total error in detection of serum lipid
Xiufang LI ; Jing QIAN ; Ying ZHAO ; Yuelin YANG ; Jiexian CAO
International Journal of Laboratory Medicine 2016;37(19):2708-2709,2712
Objective To study on the application of biological variation total allowable error in quality management of serum lipid detection .Methods The σ score ,quality goal index (QGI) ,priority improvement measures and performance evaluation of lipid accuracy criteria were evaluated ,including cholesterol (CHOL) ,triglyceride (TG) ,high density lipoprotein (HDL‐C) ,low density lipoprotein (LDL‐C) ,which included in the standard of accuracy of the Ministry of Health in 2015 .The reason for unsatisfactory re‐sults of cholesterol (CHOL) test were analyzed .Results Based on the three levels of quality specifications derived in biological var‐iation ,when the total allowable error was located at an appropriate level ,the σ score of TG ,HDL‐C ,LDL‐C reached 6 ,it was not re‐quired for improvement .While the score of CHOL performance analysis was poor ,accuracy was required to give priority to impro‐ving .When the total allowable error reached the best level ,only the σ score of TG achieved “good” in the four items ,improvement of precision was needed .When the total allowable error was located at the lowest level ,the σ score of TG ,HDL‐C ,LDL‐C was grea‐ter than 6 ,the score of performance analysis reached the “excellent” .The σ score of CHOL (2 .9) had been closen to 3σ ,accuracy was required to be corrected .Conclusion The biological variation derived total allowable error is easy to meet the requirements of the quality management in serum lipid determination by current technologies and methods .The theory of 6σ quality can reflect the performance of detection indexes ,and improve the quality of analysis effectively .
4.On the Negative Effects of the Evaluation Function of Core Journals
Yan QU ; Xiufang LI ; Zhen ZHAO ; Dianhua WANG
Journal of Kunming Medical University 2006;0(06):-
As a tool for the research of periodical literature data,core journal was initially used in optimizing selection of journals for libraries and information institutes and in providing access for readers to selection of journals.But in recent years,the primitive function of core journal has began to weaken and its negative effects to generalize,mainly in the value orientation deviation of academic periodicals and in the generalization of the evaluation function.Due to these effects,bad results have emerged in periodical circle,academia and even in scientific research management,therefore,rational cognition may be required on the functional effects of core journals.
5.Control System Design and Test of Digital Comprehensive Training Treadmill for Rats.
Xiulin XU ; Guanpo MA ; Jun ZHAO ; Xiufang HU
Journal of Biomedical Engineering 2015;32(6):1233-1238
This article presents the design of a treadmill of comprehensive training experiment for rats. The treadmill is composed of six tracks and two of them were designed as conventional plane, two were designed to swing right and left, and two were designed to swing back and forth. The power was provided by six motors. The MSP430F149 is used as core to adjust the swing rate and the grade of electric shock. The IAR for MSP430 is used to design the software. The speed of the six tracks could be adjusted between 0 and 30 m/min. The swing tracks of back and forth can be swung for 3-25 times per minute and the swing tracks of right and left for 3-32 times. The electric shock can be divided into three levels, i. e. strong, middle, and weak level for each track. The digital comprehensive training treadmill can meet different training needs, and provide experimental data for mechanism research of some related diseases.
Animals
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Equipment Design
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Exercise Test
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instrumentation
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Physical Conditioning, Animal
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instrumentation
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Rats
6.A comparative study of TPA-Nance combined anchorage and face bow in reinforcing the maxillary first molar anchorage
Hongyan ZHAO ; Wenru JIANG ; Ye ZHANG ; lin LI ; Xiufang WANG
Journal of Practical Stomatology 2000;0(06):-
0.05).Conclusions:Combined anchorage can provide maximum anchorage compared with traditional face bow and was accepted easily by patients.
7.A study of effects of impaired glucose tolerance on ventricular remodeling
Jianxi TANG ; Xiufang LIN ; Xiaoming XIE ; Zhijunn ZHOU ; Jian ZHAO ; Lan YAO ; Yusheng ZHENG
Chinese Journal of Internal Medicine 2010;49(10):841-844
Objective To evaluate the effects of impaired glucose tolerance (IGT) on ventricular remodeling. Methods Parameters of every subject including left ventricular mass ( LVM), left ventricular mass index (LVMI), E/A ratio, 75 g oral glucose tolerance test (OGTT), ambulatory blood pressure monitoring(ABPM) data including 24-hour mean systolic blood pressure(mSBP) and 24-hour mean diastolic blood pressure(mDBP) were collected. Then the relationship of IGT and myocardial remodeling related parameters were analyzed. Results The rate of diastolic dysfunction was higher in the IGT combined with hypertensive group(74% ) compared with the hypertensive group( 39% )( x2 = 6. 5, P < 0. 05 ). The rate of diastolic dysfunction was higher in the IGT group( 34% ) compared with the normal group( 10% ) (x2 = 5.2,P <0. 05). The rate of Left Ventricular Hypertrophy (LVH)in the IGT combined with hypertensive group (24%) was higher than the other three groups (Hypertension group 7%, IGT group 0, Normal group 0) (x2 =4.561,P <0.05), and there was no significance between the rest three groups (P >0.05).Stepwise multiple regression showed age and 2 Hours' Postprandial Blood Glucose were independent risk factors of E/A ratio. Conclusions These results suggested that IGT is a possible contributor to left ventricular hypertrophy and diastolic dysfunction, and is one of the histopathology of left ventricular remodeling.
8.Influence of ultrasound-targeted microbubble destruction on biofilm
Nianan HE ; Huaiwei LU ; Xinmin CHU ; Xiufang SUI ; Zhihong ZHAO ; Wenping WANG
Chinese Journal of Ultrasonography 2013;22(10):905-909
Objective To investigate whether ultrasound (US)-targeted microbubble (MB) destruction (UTMD) can influence the biofilm and bacteria in morphology.Methods Twenty-hour biofilms of Staphylococcus epidermidis RP62A were treated with US or UTMD.The acoustic intensity was 0.5-1.5W/cm2,the duty cycle was 50% and the duration was 10 minutes.After treatment,the absorbance values (A570) of biofilms stained with the crystal violet were measured to assess the biofilm density.The biofilms were observed with macroscopy and light microscopy.The biofilms were examined by confocal laserscanning microscopy (CLSM) and scanning electron microscopy (SEM).Results A thick and compact biofilm was observed in the untreated control group,and there were no obvious micropores in biofilms under macrology and light micrology.Although there were no significant changes under macroscopy in both biofilms treated with US only and UTMD with 0.5 W/cm2 acoustic intensity,interestingly,many micropores could be found under microscopy.The diameters of micropores increased with increasing acoustic intensity,and the micropores in biofilms treated with UTMD were bigger than those treated with US-only in the same condition of acoustic intensity (P <0.05).The largest diameters of micropores were up to 1 mm in biofilms treated with UTMD using 1.5 W/cm2 (P <0.05).The biofilm density (A570 value) decreased with increasing of the acoustic intensity,and the values in UTMD group of 1.5 W/cm2 were the lowest (P < 0.05).Micropores also could be observed under CLSM.There were no obvious dead bacteria in biofilms treated with US and UTMD compared with untreated control group (P >0.05).Under SEM,the shape of bacteria in biofilms treated with US and UTMD became irregular,and many rounded projection could be observed in the surface of the bacteria treated with UTMD.Conclusions US and UTMD can produce micropores in biofilms,which might help to promote antibiotic activity against biofilms
9.Expression of Survivin in colorectal cancer tissue and its relationship with clinicopathological features
Jun XUE ; Xueliang WU ; Fei GUO ; Likun WANG ; Pengjuan ZHANG ; Ming QU ; Xiufang ZHAO
Chinese Journal of Postgraduates of Medicine 2015;38(2):83-86
Objective To explore the expression of Survivin in colorectal cancer tissue and its relationship with clinicopathological features.Methods In situ hybridization was used to examine the expression of Survivin mRNA,and immunohistochemical method was used to detect the expression of Survivin protein in 60 cases of colorectal cancer and 30 cases of colorectal normal mucos tissue.The relationship of the expression of Survivin protein with clinicopathological features of colorectal cancer was analyzed.Results The positive expression rate of Survivin protein in colorectal normal mucos tissue (10.00%,3/30) was lower than that in colorectal cancer tissue (73.33%,44/60),and there was significant difference (P < 0.05).The positive expression rate of Survivin mRNA in colorectal normal mucos tissue (6.67%,2/30) was lower than that in colorectal cancer tissue (63.33%,38/60),and there was significant difference (P < 0.05).The expression of Survivin mRNA was positively correlated with depth of invasion,lymph node metastasis,liver metastasis,peritoneal micrometastasis and TNM stage in patients with colorectal cancer (P < 0.01),but had no correlation with gender,age,tumor size,location,degree of differentiation and histological type (P > 0.05).Conclusions The specific high expression of Survivin protein has a high correlation with the occurrence,development,infiltration of colorectal cancer.Survivin may be used as a marker for prognosis of patients with colorectal cancer.
10.Adipose derived mesenchymal stem cell transplantation for Alzheimer's disease
Ying NIU ; Kai GONG ; Qiang AO ; Nanming ZHAO ; Xiufang ZHANG ; Yandao GONG
Chinese Journal of Tissue Engineering Research 2009;13(27):5389-5392
Alzheimer's disease is an irreversible neurodegenerative disease characterized by progressive neuronal loss. To date, there has been no effective medicine or therapy for neurodegenerative disease. With development of stem cell technique and theory, neural stem cell transplantation has been found to be prospective in Alzheimer's disease treatment. However, it was challenged by the deficiency of autologous neural stem cell, which can bypass immunological barrier. Compared with neural stem cells, mesenchymal stem cells exhibit extensive resources, such as liver, bone marrow and adipose, and multiple differentiations into bone, muscle or adipose. Considering the easy access, the minor trauma to the patients, and the neuron differentiation potential of adipose derived mesenchymal stem cells (A-MSC), we hypothesize that A-MSC graft is a potential and innovative strategy for the treatment of Alzheimer's disease.